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The map label for this gene is menH

Identifier: 153947677

GI number: 153947677

Start: 1725827

End: 1726645

Strand: Direct

Name: menH

Synonym: YpsIP31758_1483

Alternate gene names: 153947677

Gene position: 1725827-1726645 (Clockwise)

Preceding gene: 153948006

Following gene: 153947086

Centisome position: 36.54

GC content: 54.46

Gene sequence:

>819_bases
ATGACGACACTGGCTTGTCGAAAACTGGCCCCTCATCCGGAATCGCCCCGCCATCAACACGCCGGGCCTTGGTTGGTTTG
GTTACATGGTTTGTTGGGCAGCAGGCAAGATTGGCTGCCCGTGGCACAACTGTGTGGCGATTATCCGTCATTGTTGATCG
ACCTACCGGGCCATGGACAATCCGTCTCCCTGAGCGCCGATGGTTTTGCCGATATCAGTCGCCAGTTGTCACAAACATTA
CAGGCTAACGGTATTCGCGAATACTGGCTGGCCGGCTACTCTTTAGGCGGTAGGATCGCGATGTATCACGCCTGTTATGG
TCGCCATCATGGCTTGCAGGGGTTATTGGTTGAAGGGGGAAACCTCGGGCTGGAAAACGCTGAACTACGTCAGGCGCGGT
TGCAACAAGACCGCCAATGGGCGCAGCGCTTTCGCCAGGAGCCTCTGCCACAGGTATTGGATGACTGGTATCAGCAGGCG
GTATTTGCCGATTTAGATCCACAACAACGTGAACAACTGGTGTTATTGCGGGCCGATAACCATGGCCCGGCTGTGGCCGA
GATGCTAGAGGCAACGTCACTAGGGCATCAACCGTGGTTGTTACCGGCCCTGCAACGGCTCAACGTCCCTTATACTTATT
TGTGTGGTGATCGTGACCATAAGTTCTTGCAACTGGCACAGCAATATCAATTGCCATTACACACGCTAGCCCGCGCCGGG
CACAATGCCCATCGGGCGAATCCCGGTGCTTTTGCTGCACAGGTGCTGGCATTTTTGTCACAGTCATCATGCTTACCACC
GTCATCATTATCTCGTTAA

Upstream 100 bases:

>100_bases
GGGGAACCACGCTGATTGAAGTCCAGGTGCCACCAAGTCAGGGGGCAGAAACACTGCAACAGTTAGTACAACAGGTGACA
TTAATACCGCAGGTGGCCCC

Downstream 100 bases:

>100_bases
AGGAATGTTTACTATGCTTTATCCGAGCGAAGAACAATTGTACGCCGCCATCGACTGGCTGGATTGCTCCGCCGGATTTG
AAGATATTCGTTATCACAAA

Product: acyl-CoA thioester hydrolase YfbB

Products: NA

Alternate protein names: SHCHC synthase

Number of amino acids: Translated: 272; Mature: 271

Protein sequence:

>272_residues
MTTLACRKLAPHPESPRHQHAGPWLVWLHGLLGSRQDWLPVAQLCGDYPSLLIDLPGHGQSVSLSADGFADISRQLSQTL
QANGIREYWLAGYSLGGRIAMYHACYGRHHGLQGLLVEGGNLGLENAELRQARLQQDRQWAQRFRQEPLPQVLDDWYQQA
VFADLDPQQREQLVLLRADNHGPAVAEMLEATSLGHQPWLLPALQRLNVPYTYLCGDRDHKFLQLAQQYQLPLHTLARAG
HNAHRANPGAFAAQVLAFLSQSSCLPPSSLSR

Sequences:

>Translated_272_residues
MTTLACRKLAPHPESPRHQHAGPWLVWLHGLLGSRQDWLPVAQLCGDYPSLLIDLPGHGQSVSLSADGFADISRQLSQTL
QANGIREYWLAGYSLGGRIAMYHACYGRHHGLQGLLVEGGNLGLENAELRQARLQQDRQWAQRFRQEPLPQVLDDWYQQA
VFADLDPQQREQLVLLRADNHGPAVAEMLEATSLGHQPWLLPALQRLNVPYTYLCGDRDHKFLQLAQQYQLPLHTLARAG
HNAHRANPGAFAAQVLAFLSQSSCLPPSSLSR
>Mature_271_residues
TTLACRKLAPHPESPRHQHAGPWLVWLHGLLGSRQDWLPVAQLCGDYPSLLIDLPGHGQSVSLSADGFADISRQLSQTLQ
ANGIREYWLAGYSLGGRIAMYHACYGRHHGLQGLLVEGGNLGLENAELRQARLQQDRQWAQRFRQEPLPQVLDDWYQQAV
FADLDPQQREQLVLLRADNHGPAVAEMLEATSLGHQPWLLPALQRLNVPYTYLCGDRDHKFLQLAQQYQLPLHTLARAGH
NAHRANPGAFAAQVLAFLSQSSCLPPSSLSR

Specific function: Catalyzes a proton abstraction reaction that results in 2,5-elimination of pyruvate from 2-succinyl-5-enolpyruvyl-6- hydroxy-3-cyclohexene-1-carboxylate (SEPHCHC) and the formation of 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate (SHCHC). Is also

COG id: COG0596

COG function: function code R; Predicted hydrolases or acyltransferases (alpha/beta hydrolase superfamily)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AB hydrolase superfamily. MenH family

Homologues:

Organism=Escherichia coli, GI1788598, Length=250, Percent_Identity=49.6, Blast_Score=235, Evalue=2e-63,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MENH_YERP3 (A7FGT3)

Other databases:

- EMBL:   CP000720
- RefSeq:   YP_001400461.1
- ProteinModelPortal:   A7FGT3
- SMR:   A7FGT3
- STRING:   A7FGT3
- GeneID:   5385115
- GenomeReviews:   CP000720_GR
- KEGG:   ypi:YpsIP31758_1483
- NMPDR:   fig|349747.3.peg.1049
- eggNOG:   COG0596
- HOGENOM:   HBG748003
- OMA:   ESCHPGL
- ProtClustDB:   PRK11126
- BioCyc:   YPSE349747:YPSIP31758_1483-MONOMER
- HAMAP:   MF_01660
- InterPro:   IPR000073
- InterPro:   IPR022485
- TIGRFAMs:   TIGR03695

Pfam domain/function: PF00561 Abhydrolase_1

EC number: =4.2.99.20

Molecular weight: Translated: 30372; Mature: 30241

Theoretical pI: Translated: 7.43; Mature: 7.43

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTLACRKLAPHPESPRHQHAGPWLVWLHGLLGSRQDWLPVAQLCGDYPSLLIDLPGHGQ
CCCCHHHHCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHCCCCCEEEEECCCCCC
SVSLSADGFADISRQLSQTLQANGIREYWLAGYSLGGRIAMYHACYGRHHGLQGLLVEGG
EEEECCCCHHHHHHHHHHHHHHCCCHHHHHHHHCCCCHHHHHHHHHHHCCCCCEEEEECC
NLGLENAELRQARLQQDRQWAQRFRQEPLPQVLDDWYQQAVFADLDPQQREQLVLLRADN
CCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCHHHHCCEEEEEECC
HGPAVAEMLEATSLGHQPWLLPALQRLNVPYTYLCGDRDHKFLQLAQQYQLPLHTLARAG
CCHHHHHHHHHHHCCCCCHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHCCHHHHHHHCC
HNAHRANPGAFAAQVLAFLSQSSCLPPSSLSR
CCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure 
TTLACRKLAPHPESPRHQHAGPWLVWLHGLLGSRQDWLPVAQLCGDYPSLLIDLPGHGQ
CCCHHHHCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHCCCCCEEEEECCCCCC
SVSLSADGFADISRQLSQTLQANGIREYWLAGYSLGGRIAMYHACYGRHHGLQGLLVEGG
EEEECCCCHHHHHHHHHHHHHHCCCHHHHHHHHCCCCHHHHHHHHHHHCCCCCEEEEECC
NLGLENAELRQARLQQDRQWAQRFRQEPLPQVLDDWYQQAVFADLDPQQREQLVLLRADN
CCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCHHHHCCEEEEEECC
HGPAVAEMLEATSLGHQPWLLPALQRLNVPYTYLCGDRDHKFLQLAQQYQLPLHTLARAG
CCHHHHHHHHHHHCCCCCHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHCCHHHHHHHCC
HNAHRANPGAFAAQVLAFLSQSSCLPPSSLSR
CCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA