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The map label for this gene is cobB [H]

Identifier: 153947974

GI number: 153947974

Start: 1862325

End: 1863161

Strand: Direct

Name: cobB [H]

Synonym: YpsIP31758_1604

Alternate gene names: 153947974

Gene position: 1862325-1863161 (Clockwise)

Preceding gene: 153949530

Following gene: 153948676

Centisome position: 39.43

GC content: 48.03

Gene sequence:

>837_bases
ATGCGCATTCGCCATAGGCTGTGTCGGTTTCGTAAGAGTAAGCATGTGCGGCATCAACGTTTTCGCTCCCGTATTTTTCA
TCGGGATAGTGCAGTAGCAAAAGAGATGAAGAAACCCTTTGTCGTTGTGCTTACTGGTGCTGGGATTTCTGCTGAGTCGG
GTATCCGTACTTTCCGTGCGGACGATGGTTTATGGGAAGATCACCGGGTAGAAGATGTGGCGACACCTGAAGGGTATCGG
CGTGATCCTGAGCTCGTCCAACGTTTTTATAATGAACGTCGCCGTCAGTTGCAGCAGCCAGACATAGCGCCAAATGCTGC
GCATTTCGCTCTGGCTGATTTAGAAGCCGTGCTGGGTGATAACCTGGTATTGATCACTCAAAATATTGATAACTTGCATG
AGAGAGCCGGCAGTAAGCGCGTTATTCATATGCATGGTGAATTGCTAAAAGTTCGCTGTACTCAATCTGGTCAGGTACTG
GATTGGCAAGGCGATCTCAGTGCGGATGAACGTTGTCATTGTTGCCAATTTCCCTCGCCATTACGGCCGCATATTGTTTG
GTTTGGCGAAATGCCGATGGGTATGGATGACATTTATCAGGCGCTGTCTGAGGCTGATTTCTTTATTTCAATTGGCACTT
CAGGCCATGTTTATCCTGCTGCGGGGTTTGTTCATGAATCTCATCTACATGGTGCCCATACAGTAGAACTGAATCTTGAA
CCTAGTCAGGTAGAAAGCCAGTTTGATGAGAAGCACTATGGCCTCGCCAGTAAAGTGGTACCAGAATATATTCGCGAGTT
TCTGACCACATGCGGTGAGAATCGTCAGGGGGATTGA

Upstream 100 bases:

>100_bases
TGATTCCGGGGGCGTTCGTGGTGCAGCGTTTCTGCATTTGGCAGAAAAATAATCGTCACGATTAATAACAAGACAGATAA
AGAAAAAGAGGCAAAAGGTT

Downstream 100 bases:

>100_bases
TTTCCCCGTTGGGTGCCTCAACTCATTCAGATAACTTACGGATATACCCAAATTGATTAGTGTTGCAGGTCGTCAGTCAA
TCAACACGTCCCGGCGAACT

Product: NAD-dependent deacetylase

Products: NA

Alternate protein names: Regulatory protein SIR2 homolog [H]

Number of amino acids: Translated: 278; Mature: 278

Protein sequence:

>278_residues
MRIRHRLCRFRKSKHVRHQRFRSRIFHRDSAVAKEMKKPFVVVLTGAGISAESGIRTFRADDGLWEDHRVEDVATPEGYR
RDPELVQRFYNERRRQLQQPDIAPNAAHFALADLEAVLGDNLVLITQNIDNLHERAGSKRVIHMHGELLKVRCTQSGQVL
DWQGDLSADERCHCCQFPSPLRPHIVWFGEMPMGMDDIYQALSEADFFISIGTSGHVYPAAGFVHESHLHGAHTVELNLE
PSQVESQFDEKHYGLASKVVPEYIREFLTTCGENRQGD

Sequences:

>Translated_278_residues
MRIRHRLCRFRKSKHVRHQRFRSRIFHRDSAVAKEMKKPFVVVLTGAGISAESGIRTFRADDGLWEDHRVEDVATPEGYR
RDPELVQRFYNERRRQLQQPDIAPNAAHFALADLEAVLGDNLVLITQNIDNLHERAGSKRVIHMHGELLKVRCTQSGQVL
DWQGDLSADERCHCCQFPSPLRPHIVWFGEMPMGMDDIYQALSEADFFISIGTSGHVYPAAGFVHESHLHGAHTVELNLE
PSQVESQFDEKHYGLASKVVPEYIREFLTTCGENRQGD
>Mature_278_residues
MRIRHRLCRFRKSKHVRHQRFRSRIFHRDSAVAKEMKKPFVVVLTGAGISAESGIRTFRADDGLWEDHRVEDVATPEGYR
RDPELVQRFYNERRRQLQQPDIAPNAAHFALADLEAVLGDNLVLITQNIDNLHERAGSKRVIHMHGELLKVRCTQSGQVL
DWQGDLSADERCHCCQFPSPLRPHIVWFGEMPMGMDDIYQALSEADFFISIGTSGHVYPAAGFVHESHLHGAHTVELNLE
PSQVESQFDEKHYGLASKVVPEYIREFLTTCGENRQGD

Specific function: Modulates the activities of several enzymes which are inactive in their acetylated form [H]

COG id: COG0846

COG function: function code K; NAD-dependent protein deacetylases, SIR2 family

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 deacetylase sirtuin-type domain [H]

Homologues:

Organism=Homo sapiens, GI300795542, Length=247, Percent_Identity=35.6275303643725, Blast_Score=153, Evalue=1e-37,
Organism=Homo sapiens, GI6912664, Length=304, Percent_Identity=32.8947368421053, Blast_Score=148, Evalue=5e-36,
Organism=Homo sapiens, GI13787215, Length=236, Percent_Identity=36.4406779661017, Blast_Score=145, Evalue=3e-35,
Organism=Homo sapiens, GI6912662, Length=240, Percent_Identity=30.4166666666667, Blast_Score=85, Evalue=8e-17,
Organism=Homo sapiens, GI7657575, Length=229, Percent_Identity=32.7510917030568, Blast_Score=83, Evalue=3e-16,
Organism=Escherichia coli, GI308199517, Length=233, Percent_Identity=77.2532188841202, Blast_Score=394, Evalue=1e-111,
Organism=Caenorhabditis elegans, GI17541892, Length=203, Percent_Identity=33.4975369458128, Blast_Score=86, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI71990482, Length=246, Percent_Identity=29.2682926829268, Blast_Score=84, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17567771, Length=245, Percent_Identity=26.9387755102041, Blast_Score=80, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI71990487, Length=250, Percent_Identity=29.2, Blast_Score=79, Evalue=3e-15,
Organism=Drosophila melanogaster, GI28571445, Length=122, Percent_Identity=37.7049180327869, Blast_Score=64, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003000 [H]

Pfam domain/function: PF02146 SIR2 [H]

EC number: 3.5.1.- [C]

Molecular weight: Translated: 31861; Mature: 31861

Theoretical pI: Translated: 6.99; Mature: 6.99

Prosite motif: PS50305 SIRTUIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIRHRLCRFRKSKHVRHQRFRSRIFHRDSAVAKEMKKPFVVVLTGAGISAESGIRTFRA
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCEEEEC
DDGLWEDHRVEDVATPEGYRRDPELVQRFYNERRRQLQQPDIAPNAAHFALADLEAVLGD
CCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCC
NLVLITQNIDNLHERAGSKRVIHMHGELLKVRCTQSGQVLDWQGDLSADERCHCCQFPSP
CEEEEECCHHHHHHHCCCCEEEEECCCEEEEEECCCCCEEEECCCCCCCCCCCCCCCCCC
LRPHIVWFGEMPMGMDDIYQALSEADFFISIGTSGHVYPAAGFVHESHLHGAHTVELNLE
CCCCEEEEECCCCCHHHHHHHHHCCCEEEEECCCCCEECCCCCEECCCCCCEEEEEEECC
PSQVESQFDEKHYGLASKVVPEYIREFLTTCGENRQGD
HHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MRIRHRLCRFRKSKHVRHQRFRSRIFHRDSAVAKEMKKPFVVVLTGAGISAESGIRTFRA
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCEEEEC
DDGLWEDHRVEDVATPEGYRRDPELVQRFYNERRRQLQQPDIAPNAAHFALADLEAVLGD
CCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCC
NLVLITQNIDNLHERAGSKRVIHMHGELLKVRCTQSGQVLDWQGDLSADERCHCCQFPSP
CEEEEECCHHHHHHHCCCCEEEEECCCEEEEEECCCCCEEEECCCCCCCCCCCCCCCCCC
LRPHIVWFGEMPMGMDDIYQALSEADFFISIGTSGHVYPAAGFVHESHLHGAHTVELNLE
CCCCEEEEECCCCCHHHHHHHHHCCCEEEEECCCCCEECCCCCEECCCCCCEEEEEEECC
PSQVESQFDEKHYGLASKVVPEYIREFLTTCGENRQGD
HHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11586360; 12142430 [H]