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The map label for this gene is nagK

Identifier: 153949530

GI number: 153949530

Start: 1861362

End: 1862276

Strand: Direct

Name: nagK

Synonym: YpsIP31758_1603

Alternate gene names: 153949530

Gene position: 1861362-1862276 (Clockwise)

Preceding gene: 153949780

Following gene: 153947974

Centisome position: 39.41

GC content: 50.6

Gene sequence:

>915_bases
ATGTATTACGGTTTTGATATGGGCGGGACCAAAATAGAGCTAGGCGTTTTTGATGAAAACCTGCAGCGAATATGGCATAA
GCGAGTGCCTACGCCACGTGAGGATTACCCGCAGTTACTGCAAATATTGCGGGATTTAACCGAGGAAGCCGATACTTATT
GCGGTGTTCAAGGCAGTGTGGGTATCGGTATTCCAGGGCTACCCAATGCGGATGATGGCACGGTATTTACTGCGAATGTA
CCGTCTGCGATGGGGCAACCATTGCAGGCTGATCTTTCCCGCTTGATCCAGCGTGAAGTCCGTATTGATAACGATGCCAA
CTGTTTTGCTCTCTCCGAAGCCTGGGACCCTGAGTTTCGCACTTACCCAACGGTGCTGGGGCTTATTCTCGGCACCGGTG
TTGGTGGTGGGTTAATTGTTAATGGCAGTATTGTCAGTGGGCGTAACCACATCACTGGCGAGTTCGGTCATTTCCGTTTA
CCAGTAGATGCACTGGATATTCTTGGTGCCGATATTCCCCACGTTCCCTGTGGTTGTGGCCATCGCGGTTGTATAGAGAA
TTATATTTCTGGGCGCGGTTTCGAGTGGATGTACAGTCATTTTTATCAACATACCTTACCTGCCACCGACATTATTGCCC
ATTATGCTGCGGGTGAGCCAAAGGCGGTGGCACATGTTGAGCGCTTTATGGATGTGCTGGCGGTCTGTTTGGGCAATCTT
TTGACTATGCTCGATCCCCATTTGGTCGTGGTGGGCGGGGGGTTGTCTAATTTCGAAAAAATCTATCAGGAGCTGCCAAA
GCGCCTGCCTGCGCATTTATTGCGAGTGGCCCGGTTACCGCGTATCGAAAAAGCGCGTTATGGTGATTCCGGGGGCGTTC
GTGGTGCAGCGTTTCTGCATTTGGCAGAAAAATAA

Upstream 100 bases:

>100_bases
GGTGCTCAGCTTGATTGCCAGTTGGTATCCGGCACGTCGCGCCAGTCGTATTGATCCGGCACGGGTCCTCAGCGGGCAGT
AATTGAAGAAAGGCAGGCTT

Downstream 100 bases:

>100_bases
TCGTCACGATTAATAACAAGACAGATAAAGAAAAAGAGGCAAAAGGTTATGCGCATTCGCCATAGGCTGTGTCGGTTTCG
TAAGAGTAAGCATGTGCGGC

Product: N-acetyl-D-glucosamine kinase

Products: NA

Alternate protein names: GlcNAc kinase

Number of amino acids: Translated: 304; Mature: 304

Protein sequence:

>304_residues
MYYGFDMGGTKIELGVFDENLQRIWHKRVPTPREDYPQLLQILRDLTEEADTYCGVQGSVGIGIPGLPNADDGTVFTANV
PSAMGQPLQADLSRLIQREVRIDNDANCFALSEAWDPEFRTYPTVLGLILGTGVGGGLIVNGSIVSGRNHITGEFGHFRL
PVDALDILGADIPHVPCGCGHRGCIENYISGRGFEWMYSHFYQHTLPATDIIAHYAAGEPKAVAHVERFMDVLAVCLGNL
LTMLDPHLVVVGGGLSNFEKIYQELPKRLPAHLLRVARLPRIEKARYGDSGGVRGAAFLHLAEK

Sequences:

>Translated_304_residues
MYYGFDMGGTKIELGVFDENLQRIWHKRVPTPREDYPQLLQILRDLTEEADTYCGVQGSVGIGIPGLPNADDGTVFTANV
PSAMGQPLQADLSRLIQREVRIDNDANCFALSEAWDPEFRTYPTVLGLILGTGVGGGLIVNGSIVSGRNHITGEFGHFRL
PVDALDILGADIPHVPCGCGHRGCIENYISGRGFEWMYSHFYQHTLPATDIIAHYAAGEPKAVAHVERFMDVLAVCLGNL
LTMLDPHLVVVGGGLSNFEKIYQELPKRLPAHLLRVARLPRIEKARYGDSGGVRGAAFLHLAEK
>Mature_304_residues
MYYGFDMGGTKIELGVFDENLQRIWHKRVPTPREDYPQLLQILRDLTEEADTYCGVQGSVGIGIPGLPNADDGTVFTANV
PSAMGQPLQADLSRLIQREVRIDNDANCFALSEAWDPEFRTYPTVLGLILGTGVGGGLIVNGSIVSGRNHITGEFGHFRL
PVDALDILGADIPHVPCGCGHRGCIENYISGRGFEWMYSHFYQHTLPATDIIAHYAAGEPKAVAHVERFMDVLAVCLGNL
LTMLDPHLVVVGGGLSNFEKIYQELPKRLPAHLLRVARLPRIEKARYGDSGGVRGAAFLHLAEK

Specific function: Catalyzes the phosphorylation of N-acetyl-D-glucosamine (GlcNAc) derived from cell-wall degradation, yielding GlcNAc-6-P

COG id: COG1940

COG function: function code KG; Transcriptional regulator/sugar kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ROK (nagC/xylR) family. NagK subfamily

Homologues:

Organism=Escherichia coli, GI1787363, Length=303, Percent_Identity=64.3564356435644, Blast_Score=410, Evalue=1e-116,
Organism=Escherichia coli, GI87081733, Length=299, Percent_Identity=39.7993311036789, Blast_Score=211, Evalue=5e-56,
Organism=Escherichia coli, GI1790522, Length=305, Percent_Identity=26.2295081967213, Blast_Score=70, Evalue=2e-13,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NAGK_YERP3 (A7FH52)

Other databases:

- EMBL:   CP000720
- RefSeq:   YP_001400580.1
- ProteinModelPortal:   A7FH52
- SMR:   A7FH52
- STRING:   A7FH52
- GeneID:   5386543
- GenomeReviews:   CP000720_GR
- KEGG:   ypi:YpsIP31758_1603
- NMPDR:   fig|349747.3.peg.932
- eggNOG:   COG1940
- HOGENOM:   HBG730260
- OMA:   GMEDADD
- ProtClustDB:   PRK13310
- BioCyc:   YPSE349747:YPSIP31758_1603-MONOMER
- HAMAP:   MF_01271
- InterPro:   IPR000600

Pfam domain/function: PF00480 ROK

EC number: =2.7.1.59

Molecular weight: Translated: 33303; Mature: 33303

Theoretical pI: Translated: 6.07; Mature: 6.07

Prosite motif: PS01125 ROK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYYGFDMGGTKIELGVFDENLQRIWHKRVPTPREDYPQLLQILRDLTEEADTYCGVQGSV
CCEEECCCCCEEEEEECHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCC
GIGIPGLPNADDGTVFTANVPSAMGQPLQADLSRLIQREVRIDNDANCFALSEAWDPEFR
CCCCCCCCCCCCCEEEEECCCHHHCCCHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCHH
TYPTVLGLILGTGVGGGLIVNGSIVSGRNHITGEFGHFRLPVDALDILGADIPHVPCGCG
HHHHHHHHHHHCCCCCCEEEECEEECCCCCCCCCCCCEECCHHHHHHHCCCCCCCCCCCC
HRGCIENYISGRGFEWMYSHFYQHTLPATDIIAHYAAGEPKAVAHVERFMDVLAVCLGNL
CHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHH
LTMLDPHLVVVGGGLSNFEKIYQELPKRLPAHLLRVARLPRIEKARYGDSGGVRGAAFLH
HHHHCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCHHEEEE
LAEK
ECCC
>Mature Secondary Structure
MYYGFDMGGTKIELGVFDENLQRIWHKRVPTPREDYPQLLQILRDLTEEADTYCGVQGSV
CCEEECCCCCEEEEEECHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCC
GIGIPGLPNADDGTVFTANVPSAMGQPLQADLSRLIQREVRIDNDANCFALSEAWDPEFR
CCCCCCCCCCCCCEEEEECCCHHHCCCHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCHH
TYPTVLGLILGTGVGGGLIVNGSIVSGRNHITGEFGHFRLPVDALDILGADIPHVPCGCG
HHHHHHHHHHHCCCCCCEEEECEEECCCCCCCCCCCCEECCHHHHHHHCCCCCCCCCCCC
HRGCIENYISGRGFEWMYSHFYQHTLPATDIIAHYAAGEPKAVAHVERFMDVLAVCLGNL
CHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHH
LTMLDPHLVVVGGGLSNFEKIYQELPKRLPAHLLRVARLPRIEKARYGDSGGVRGAAFLH
HHHHCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCHHEEEE
LAEK
ECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA