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The map label for this gene is ywtE [H]

Identifier: 152976473

GI number: 152976473

Start: 2845339

End: 2846124

Strand: Reverse

Name: ywtE [H]

Synonym: Bcer98_2762

Alternate gene names: 152976473

Gene position: 2846124-2845339 (Counterclockwise)

Preceding gene: 152976474

Following gene: 152976471

Centisome position: 69.64

GC content: 34.48

Gene sequence:

>786_bases
ATGATTAAATTATTTGTAAGTGATTTAGATGATACACTCGTTTATAATGTGAGCCATATGCACGAAGCGGATAAAAAAGC
GATTCAGTGGTTAGCGGATAAAGGAACAAATATTTGTTTTGCTTCAGGTCGTTTTACACATCGAATTCACGAGGTAGTAG
GTAGCTTTGAATTTCCATATTACATAACCGGTCTCAATGGTGCTATCATGTTGTTACCAGATGGACAAATATTTCATGAA
TCAAAATTTGATAATAAAGTTGTCCAGGAAATATATCAATATATTCATGAAAAACAACTAGCTGATATTGTCTGTGCAAA
AGAGAACAGATATACAAAAAAGAAAAATGAACATCATCACAATTTTGAGAAGGATATGGGAGTCCAGATTACTGAAATTG
AGACGCTTGAGGATGAATTTGGTAAAACAGTTTATCCAGCAAAATTATTTATATATGGAGAAGAAAAAAAGATTGAAAGG
TTAGACCGAGAGCTGAGAGAGAAATTTCATAAGGTTGCTGAAGTTGTGATTTCTGGAAAGTGTTATGTTGATATTATGCC
GACAGGAGTCAGTAAAGGGAATGCTTTGAAACGTTTAATCAATCATTTAGGGATTGAAGAGAATGAAGTTGCTTGTATCG
GAGACTCCTTTAACGATATTTCGATGTTCGCCGTAACGCCACATTCATTCACACTTCATCATGCCCATCCGTATGTGAAA
GAGAAAGCGAGTTATATTGTACGTTCAGTAGAAGAAGCTATTATGAAATTACCATTGCTTGCATAA

Upstream 100 bases:

>100_bases
CTCTGCTTATTTTTCATGATAAATTTATTGTTTTTTAACAAAAAGTCAACATTTTTTTCGTACACTTAGAGTAGATATTG
TGTATGAAAGGGGAAGAGGC

Downstream 100 bases:

>100_bases
GAGAAGAAGCTCGTCATTTGACGAGCTTCTTATTATTTAAATAATGATTTTATAAAATCGATGATACTAGAGAAAAAGTC
TTTTACTTTATCTAGAAAGC

Product: Cof-like hydrolase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 261; Mature: 261

Protein sequence:

>261_residues
MIKLFVSDLDDTLVYNVSHMHEADKKAIQWLADKGTNICFASGRFTHRIHEVVGSFEFPYYITGLNGAIMLLPDGQIFHE
SKFDNKVVQEIYQYIHEKQLADIVCAKENRYTKKKNEHHHNFEKDMGVQITEIETLEDEFGKTVYPAKLFIYGEEKKIER
LDRELREKFHKVAEVVISGKCYVDIMPTGVSKGNALKRLINHLGIEENEVACIGDSFNDISMFAVTPHSFTLHHAHPYVK
EKASYIVRSVEEAIMKLPLLA

Sequences:

>Translated_261_residues
MIKLFVSDLDDTLVYNVSHMHEADKKAIQWLADKGTNICFASGRFTHRIHEVVGSFEFPYYITGLNGAIMLLPDGQIFHE
SKFDNKVVQEIYQYIHEKQLADIVCAKENRYTKKKNEHHHNFEKDMGVQITEIETLEDEFGKTVYPAKLFIYGEEKKIER
LDRELREKFHKVAEVVISGKCYVDIMPTGVSKGNALKRLINHLGIEENEVACIGDSFNDISMFAVTPHSFTLHHAHPYVK
EKASYIVRSVEEAIMKLPLLA
>Mature_261_residues
MIKLFVSDLDDTLVYNVSHMHEADKKAIQWLADKGTNICFASGRFTHRIHEVVGSFEFPYYITGLNGAIMLLPDGQIFHE
SKFDNKVVQEIYQYIHEKQLADIVCAKENRYTKKKNEHHHNFEKDMGVQITEIETLEDEFGKTVYPAKLFIYGEEKKIER
LDRELREKFHKVAEVVISGKCYVDIMPTGVSKGNALKRLINHLGIEENEVACIGDSFNDISMFAVTPHSFTLHHAHPYVK
EKASYIVRSVEEAIMKLPLLA

Specific function: Unknown

COG id: COG0561

COG function: function code R; Predicted hydrolases of the HAD superfamily

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HAD-like hydrolase superfamily. Cof family [H]

Homologues:

Organism=Escherichia coli, GI87081741, Length=246, Percent_Identity=20.7317073170732, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI2367265, Length=267, Percent_Identity=27.3408239700375, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI48994981, Length=246, Percent_Identity=22.3577235772358, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1787043, Length=249, Percent_Identity=22.4899598393574, Blast_Score=61, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005834
- InterPro:   IPR023214
- InterPro:   IPR006379
- InterPro:   IPR000150 [H]

Pfam domain/function: PF00702 Hydrolase [H]

EC number: NA

Molecular weight: Translated: 30032; Mature: 30032

Theoretical pI: Translated: 6.57; Mature: 6.57

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKLFVSDLDDTLVYNVSHMHEADKKAIQWLADKGTNICFASGRFTHRIHEVVGSFEFPY
CEEEEEECCCCHHEEEHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHCCCCCCE
YITGLNGAIMLLPDGQIFHESKFDNKVVQEIYQYIHEKQLADIVCAKENRYTKKKNEHHH
EEEECCCEEEEEECCCEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
NFEKDMGVQITEIETLEDEFGKTVYPAKLFIYGEEKKIERLDRELREKFHKVAEVVISGK
CCHHHHCCEEEEHHHHHHHHCCEECCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHCCC
CYVDIMPTGVSKGNALKRLINHLGIEENEVACIGDSFNDISMFAVTPHSFTLHHAHPYVK
EEEEEEECCCCCCHHHHHHHHHCCCCCCCEEEECCCCCCEEEEEECCCCEEEECCCCHHH
EKASYIVRSVEEAIMKLPLLA
HHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MIKLFVSDLDDTLVYNVSHMHEADKKAIQWLADKGTNICFASGRFTHRIHEVVGSFEFPY
CEEEEEECCCCHHEEEHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHCCCCCCE
YITGLNGAIMLLPDGQIFHESKFDNKVVQEIYQYIHEKQLADIVCAKENRYTKKKNEHHH
EEEECCCEEEEEECCCEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
NFEKDMGVQITEIETLEDEFGKTVYPAKLFIYGEEKKIERLDRELREKFHKVAEVVISGK
CCHHHHCCEEEEHHHHHHHHCCEECCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHCCC
CYVDIMPTGVSKGNALKRLINHLGIEENEVACIGDSFNDISMFAVTPHSFTLHHAHPYVK
EEEEEEECCCCCCHHHHHHHHHCCCCCCCEEEECCCCCCEEEEEECCCCEEEECCCCHHH
EKASYIVRSVEEAIMKLPLLA
HHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9353933; 9384377 [H]