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The map label for this gene is murE [H]

Identifier: 152976276

GI number: 152976276

Start: 2664219

End: 2665694

Strand: Reverse

Name: murE [H]

Synonym: Bcer98_2564

Alternate gene names: 152976276

Gene position: 2665694-2664219 (Counterclockwise)

Preceding gene: 152976277

Following gene: 152976275

Centisome position: 65.22

GC content: 37.74

Gene sequence:

>1476_bases
ATGAAGTTGCACACACTTGTATCATGTTTGCATGATTTTCCAGTTGTTCCAAAAGAAAATCCAGAAATTACATCTATTGA
AGCAGATTCACGTAAGGTAACAAATGGAAGCTTATTTGTATGTATGAAAGGATATACGGTTGATAGCCATGATTTTGCTG
AGCAAGCAGCCGCGCAAGGTGCGGCTGCTATTGTTGCGGAAAGACCGATTGATGTTGACGTTCCAGTTGTACTTGTGAAA
GATACTTTTCGTTCATTAGCTGTGTTAGCGGACTATTTTTATGAGCAGCCAACACACAAATTGCACCTCATTGGTATTAC
AGGTACAAATGGAAAGACAACAACATCGCACATTATAGATGAAATTATGCGAGCACACGGTCATAAAACAGGACTAATTG
GAACAATTAATATGAAAATTGGCGATGAAACATTTGCAGTGAAAAATACAACACCTGATGCATTGACGCTTCAACAAGCA
TTTCACCGTATGGTGGAAGAAAATGTGAATAGTGCAGTTATGGAAGTTTCTTCACATGCATTAGATCGTGGTCGTGTACA
TGGTTGTGATTATGATGTAGCAGTATTTACAAATTTAACACAAGATCATTTAGATTATCACAAGACGATGGAAGAGTATA
AGCATGCTAAAGGATTACTTTTTGCTCAGCTTGGTAATAGCTATCATCATAATCGCGAAAAGTATGCCGTATTAAATAAT
GATGATGCAGCAACAGAAGCGTATATAAGAAGTACAGCAGCTACGGTTGTTACGTATGGAATTGATACCCATAGTGATGT
AATGGCGAAGGATATCGTTATGACAAGCGGCGGTACGACATTTACTCTTGTTACACCGCACGAAAGTAAAAAAATTACGA
TGAAATTAATTGGGAAGTTTAATGTATATAATGCTTTAGCCGCTACAGCAGCAGGACTTGTATCTGGTGTATCTCTGCAA
ACAATCATAGATGTTTTAGAACATTTAACTGGTGTTGCAGGCCGATTTGAAGTTGTAGATGGTGGGCAGGATTATACAGT
AATTGTTGATTATGCGCATACGCCTGATAGTTTAGAAAATGTATTAACAACGGCGAAGCAATTTGCAAAAGGTAATGTAT
ATTGCATCGTTGGCTGCGGTGGTGATCGTGATAGAACGAAAAGACCGATTATGGCAGGCGTTGCGACAGAATATGCAACC
CATGCCATCTTAACTTCAGATAATCCGAGAAGTGAAGATCCACAAGCTATTTTGGATGATATGTTAACTGGTGTAAAAGG
GAATAATTATGAAGTAATTGTTGATAGGAAGAAAGCAATTTTTCATGCTATTTCTGAAGCGCAAGCGGAAGATGTTATTA
TTATTGCTGGAAAGGGTCATGAAACGTATCAAATTATTGGAAAAGAAGTACATCATTTTGACGATCGTGAAGTTGCTAAA
GAAGCAATCACAGAACGTTTAAACAACAGAGAGTAA

Upstream 100 bases:

>100_bases
TTGTTAGACCATGGGGTGTTACCTCCCGGTTTAAATGAGAAGTGGGAGGTAACTTATTTATAGAAAAGATAAATAAACAA
TGTAGAGAGGGTTTAGTGAA

Downstream 100 bases:

>100_bases
CAACATGAGAGGGGGACTACATGTGCTTGAACAAGGTTTATTAGTAACGGCTGGGGTAGCGTTCTTAATTTCAGTTGCCC
TTTCGCCATTGTTTATTCCG

Product: UDP-N-acetylmuramoylalanyl-D-glutamate--2, 6-diaminopimelate ligase

Products: NA

Alternate protein names: Meso-A2pm-adding enzyme; Meso-diaminopimelate-adding enzyme; UDP-MurNAc-L-Ala-D-Glu:meso-diaminopimelate ligase; UDP-MurNAc-tripeptide synthetase; UDP-N-acetylmuramyl-tripeptide synthetase [H]

Number of amino acids: Translated: 491; Mature: 491

Protein sequence:

>491_residues
MKLHTLVSCLHDFPVVPKENPEITSIEADSRKVTNGSLFVCMKGYTVDSHDFAEQAAAQGAAAIVAERPIDVDVPVVLVK
DTFRSLAVLADYFYEQPTHKLHLIGITGTNGKTTTSHIIDEIMRAHGHKTGLIGTINMKIGDETFAVKNTTPDALTLQQA
FHRMVEENVNSAVMEVSSHALDRGRVHGCDYDVAVFTNLTQDHLDYHKTMEEYKHAKGLLFAQLGNSYHHNREKYAVLNN
DDAATEAYIRSTAATVVTYGIDTHSDVMAKDIVMTSGGTTFTLVTPHESKKITMKLIGKFNVYNALAATAAGLVSGVSLQ
TIIDVLEHLTGVAGRFEVVDGGQDYTVIVDYAHTPDSLENVLTTAKQFAKGNVYCIVGCGGDRDRTKRPIMAGVATEYAT
HAILTSDNPRSEDPQAILDDMLTGVKGNNYEVIVDRKKAIFHAISEAQAEDVIIIAGKGHETYQIIGKEVHHFDDREVAK
EAITERLNNRE

Sequences:

>Translated_491_residues
MKLHTLVSCLHDFPVVPKENPEITSIEADSRKVTNGSLFVCMKGYTVDSHDFAEQAAAQGAAAIVAERPIDVDVPVVLVK
DTFRSLAVLADYFYEQPTHKLHLIGITGTNGKTTTSHIIDEIMRAHGHKTGLIGTINMKIGDETFAVKNTTPDALTLQQA
FHRMVEENVNSAVMEVSSHALDRGRVHGCDYDVAVFTNLTQDHLDYHKTMEEYKHAKGLLFAQLGNSYHHNREKYAVLNN
DDAATEAYIRSTAATVVTYGIDTHSDVMAKDIVMTSGGTTFTLVTPHESKKITMKLIGKFNVYNALAATAAGLVSGVSLQ
TIIDVLEHLTGVAGRFEVVDGGQDYTVIVDYAHTPDSLENVLTTAKQFAKGNVYCIVGCGGDRDRTKRPIMAGVATEYAT
HAILTSDNPRSEDPQAILDDMLTGVKGNNYEVIVDRKKAIFHAISEAQAEDVIIIAGKGHETYQIIGKEVHHFDDREVAK
EAITERLNNRE
>Mature_491_residues
MKLHTLVSCLHDFPVVPKENPEITSIEADSRKVTNGSLFVCMKGYTVDSHDFAEQAAAQGAAAIVAERPIDVDVPVVLVK
DTFRSLAVLADYFYEQPTHKLHLIGITGTNGKTTTSHIIDEIMRAHGHKTGLIGTINMKIGDETFAVKNTTPDALTLQQA
FHRMVEENVNSAVMEVSSHALDRGRVHGCDYDVAVFTNLTQDHLDYHKTMEEYKHAKGLLFAQLGNSYHHNREKYAVLNN
DDAATEAYIRSTAATVVTYGIDTHSDVMAKDIVMTSGGTTFTLVTPHESKKITMKLIGKFNVYNALAATAAGLVSGVSLQ
TIIDVLEHLTGVAGRFEVVDGGQDYTVIVDYAHTPDSLENVLTTAKQFAKGNVYCIVGCGGDRDRTKRPIMAGVATEYAT
HAILTSDNPRSEDPQAILDDMLTGVKGNNYEVIVDRKKAIFHAISEAQAEDVIIIAGKGHETYQIIGKEVHHFDDREVAK
EAITERLNNRE

Specific function: Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan [H]

COG id: COG0769

COG function: function code M; UDP-N-acetylmuramyl tripeptide synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family. MurE subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786273, Length=481, Percent_Identity=35.5509355509356, Blast_Score=270, Evalue=1e-73,
Organism=Escherichia coli, GI1786274, Length=328, Percent_Identity=29.5731707317073, Blast_Score=108, Evalue=9e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005761 [H]

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.13 [H]

Molecular weight: Translated: 53808; Mature: 53808

Theoretical pI: Translated: 5.97; Mature: 5.97

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLHTLVSCLHDFPVVPKENPEITSIEADSRKVTNGSLFVCMKGYTVDSHDFAEQAAAQG
CCHHHHHHHHHHCCCCCCCCCCEEEEECCCCEECCCEEEEEEECCEECCHHHHHHHHHCC
AAAIVAERPIDVDVPVVLVKDTFRSLAVLADYFYEQPTHKLHLIGITGTNGKTTTSHIID
CEEEEECCCCCCCCCEEEEHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCHHHHHHH
EIMRAHGHKTGLIGTINMKIGDETFAVKNTTPDALTLQQAFHRMVEENVNSAVMEVSSHA
HHHHHCCCCCCEEEEEEEEECCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
LDRGRVHGCDYDVAVFTNLTQDHLDYHKTMEEYKHAKGLLFAQLGNSYHHNREKYAVLNN
HHCCCCCCCCCCHHEEECCCHHHHHHHHHHHHHHHHCCEEEEHHCCCCCCCCCEEEEECC
DDAATEAYIRSTAATVVTYGIDTHSDVMAKDIVMTSGGTTFTLVTPHESKKITMKLIGKF
CCHHHHHHHHHHHEEEEEECCCCCCHHHHHHEEEECCCCEEEEECCCCCCEEEEEEEECC
NVYNALAATAAGLVSGVSLQTIIDVLEHLTGVAGRFEVVDGGQDYTVIVDYAHTPDSLEN
CHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCEEEEEECCCCEEEEEEECCCCHHHHH
VLTTAKQFAKGNVYCIVGCGGDRDRTKRPIMAGVATEYATHAILTSDNPRSEDPQAILDD
HHHHHHHHHCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHEEEEECCCCCCCCHHHHHHH
MLTGVKGNNYEVIVDRKKAIFHAISEAQAEDVIIIAGKGHETYQIIGKEVHHFDDREVAK
HHHCCCCCCEEEEEECHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHCCCHHHHHH
EAITERLNNRE
HHHHHHHCCCC
>Mature Secondary Structure
MKLHTLVSCLHDFPVVPKENPEITSIEADSRKVTNGSLFVCMKGYTVDSHDFAEQAAAQG
CCHHHHHHHHHHCCCCCCCCCCEEEEECCCCEECCCEEEEEEECCEECCHHHHHHHHHCC
AAAIVAERPIDVDVPVVLVKDTFRSLAVLADYFYEQPTHKLHLIGITGTNGKTTTSHIID
CEEEEECCCCCCCCCEEEEHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCHHHHHHH
EIMRAHGHKTGLIGTINMKIGDETFAVKNTTPDALTLQQAFHRMVEENVNSAVMEVSSHA
HHHHHCCCCCCEEEEEEEEECCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
LDRGRVHGCDYDVAVFTNLTQDHLDYHKTMEEYKHAKGLLFAQLGNSYHHNREKYAVLNN
HHCCCCCCCCCCHHEEECCCHHHHHHHHHHHHHHHHCCEEEEHHCCCCCCCCCEEEEECC
DDAATEAYIRSTAATVVTYGIDTHSDVMAKDIVMTSGGTTFTLVTPHESKKITMKLIGKF
CCHHHHHHHHHHHEEEEEECCCCCCHHHHHHEEEECCCCEEEEECCCCCCEEEEEEEECC
NVYNALAATAAGLVSGVSLQTIIDVLEHLTGVAGRFEVVDGGQDYTVIVDYAHTPDSLEN
CHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCEEEEEECCCCEEEEEEECCCCHHHHH
VLTTAKQFAKGNVYCIVGCGGDRDRTKRPIMAGVATEYATHAILTSDNPRSEDPQAILDD
HHHHHHHHHCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHEEEEECCCCCCCCHHHHHHH
MLTGVKGNNYEVIVDRKKAIFHAISEAQAEDVIIIAGKGHETYQIIGKEVHHFDDREVAK
HHHCCCCCCEEEEEECHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHCCCHHHHHH
EAITERLNNRE
HHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12721629 [H]