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The map label for this gene is purN [H]

Identifier: 152974117

GI number: 152974117

Start: 317721

End: 318308

Strand: Direct

Name: purN [H]

Synonym: Bcer98_0276

Alternate gene names: 152974117

Gene position: 317721-318308 (Clockwise)

Preceding gene: 152974116

Following gene: 152974118

Centisome position: 7.77

GC content: 37.93

Gene sequence:

>588_bases
ATGAATAGATTGGCAATCTTTGCCTCTGGAAGCGGATCTAACTTTCAAGCATTCGTTAACGCAGTAGAAGAAAATAGATT
ACATGCTGAAATTAGTTTGCTAGTATGTGATCAACCCGAGGCGCGTGTAATCGGAAGAGCGCATTATCATCATGTTCCAT
GCTTTGCTTTTTCGGCGAAAGCATATGAATCAAAGGAAGCGTTTGAAAAAGAGATATTAAAAAAATTACGAGAATACGAG
ATTGATTTTGTTATTTTAGCGGGATATATGCGTCTTATCGGACCGACATTGCTAGAAGCATATGGGGGAAAAATCATTAA
TATTCATCCATCGTTATTACCGAGCTTTCCAGGAAAAGATGCTATTGGCCAAGCGTTAAAAGCAGGGGTGGGAGTAACTG
GAGTAACGATTCACTATGTAGATGCTGGTATGGATACAGGACCGGTTATTGCACAAGAAGCAGTGCAGGTTTCTGAAAAT
GATACGAGAGATAGTTTGCAAAAGAAAATTCAACAAGTGGAACATCGTTTATATGTAAATACGGTAAATAAAATCATTCA
ATCTATGAAAGAACGAACTGTGAAATAG

Upstream 100 bases:

>100_bases
AAAGCGGTTGTTCGTCTTCTTGAAGAACAAGGAGAAAAAGCGTATATCATTGGACGCACTGTAAAAGGATCAGGTGTTGC
TTTTAATGGAGGAATAGATC

Downstream 100 bases:

>100_bases
GGGTGTAAAAGAAATGAAAAAGCGTGCATTAGTAAGTGTTTCTAATAAAACAGGAGTAGTAGAATTCGTGAAAGGTTTGC
TTGAACAAGGAATTGAAGTG

Product: phosphoribosylglycinamide formyltransferase

Products: NA

Alternate protein names: 5'-phosphoribosylglycinamide transformylase; GAR transformylase; GART [H]

Number of amino acids: Translated: 195; Mature: 195

Protein sequence:

>195_residues
MNRLAIFASGSGSNFQAFVNAVEENRLHAEISLLVCDQPEARVIGRAHYHHVPCFAFSAKAYESKEAFEKEILKKLREYE
IDFVILAGYMRLIGPTLLEAYGGKIINIHPSLLPSFPGKDAIGQALKAGVGVTGVTIHYVDAGMDTGPVIAQEAVQVSEN
DTRDSLQKKIQQVEHRLYVNTVNKIIQSMKERTVK

Sequences:

>Translated_195_residues
MNRLAIFASGSGSNFQAFVNAVEENRLHAEISLLVCDQPEARVIGRAHYHHVPCFAFSAKAYESKEAFEKEILKKLREYE
IDFVILAGYMRLIGPTLLEAYGGKIINIHPSLLPSFPGKDAIGQALKAGVGVTGVTIHYVDAGMDTGPVIAQEAVQVSEN
DTRDSLQKKIQQVEHRLYVNTVNKIIQSMKERTVK
>Mature_195_residues
MNRLAIFASGSGSNFQAFVNAVEENRLHAEISLLVCDQPEARVIGRAHYHHVPCFAFSAKAYESKEAFEKEILKKLREYE
IDFVILAGYMRLIGPTLLEAYGGKIINIHPSLLPSFPGKDAIGQALKAGVGVTGVTIHYVDAGMDTGPVIAQEAVQVSEN
DTRDSLQKKIQQVEHRLYVNTVNKIIQSMKERTVK

Specific function: De novo purine biosynthesis; third step. [C]

COG id: COG0299

COG function: function code F; Folate-dependent phosphoribosylglycinamide formyltransferase PurN

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GART family [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=176, Percent_Identity=37.5, Blast_Score=139, Evalue=2e-33,
Organism=Homo sapiens, GI209869995, Length=176, Percent_Identity=37.5, Blast_Score=139, Evalue=2e-33,
Organism=Homo sapiens, GI209869993, Length=176, Percent_Identity=37.5, Blast_Score=139, Evalue=2e-33,
Organism=Escherichia coli, GI1788846, Length=178, Percent_Identity=34.8314606741573, Blast_Score=130, Evalue=4e-32,
Organism=Escherichia coli, GI1787483, Length=123, Percent_Identity=31.7073170731707, Blast_Score=84, Evalue=5e-18,
Organism=Caenorhabditis elegans, GI17567511, Length=190, Percent_Identity=32.1052631578947, Blast_Score=114, Evalue=2e-26,
Organism=Saccharomyces cerevisiae, GI6320616, Length=212, Percent_Identity=25.4716981132075, Blast_Score=71, Evalue=9e-14,
Organism=Drosophila melanogaster, GI24582400, Length=186, Percent_Identity=34.4086021505376, Blast_Score=140, Evalue=4e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002376
- InterPro:   IPR001555
- InterPro:   IPR004607 [H]

Pfam domain/function: PF00551 Formyl_trans_N [H]

EC number: =2.1.2.2 [H]

Molecular weight: Translated: 21597; Mature: 21597

Theoretical pI: Translated: 7.70; Mature: 7.70

Prosite motif: PS00373 GART

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRLAIFASGSGSNFQAFVNAVEENRLHAEISLLVCDQPEARVIGRAHYHHVPCFAFSAK
CCCEEEEECCCCCCHHHHHHHHHHCCCEEEEEEEEECCCCHHHHHHHHHCCCCHHEECHH
AYESKEAFEKEILKKLREYEIDFVILAGYMRLIGPTLLEAYGGKIINIHPSLLPSFPGKD
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCEEEEECHHHCCCCCCHH
AIGQALKAGVGVTGVTIHYVDAGMDTGPVIAQEAVQVSENDTRDSLQKKIQQVEHRLYVN
HHHHHHHHCCCCCEEEEEEEECCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHH
TVNKIIQSMKERTVK
HHHHHHHHHHHHCCC
>Mature Secondary Structure
MNRLAIFASGSGSNFQAFVNAVEENRLHAEISLLVCDQPEARVIGRAHYHHVPCFAFSAK
CCCEEEEECCCCCCHHHHHHHHHHCCCEEEEEEEEECCCCHHHHHHHHHCCCCHHEECHH
AYESKEAFEKEILKKLREYEIDFVILAGYMRLIGPTLLEAYGGKIINIHPSLLPSFPGKD
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCEEEEECHHHCCCCCCHH
AIGQALKAGVGVTGVTIHYVDAGMDTGPVIAQEAVQVSENDTRDSLQKKIQQVEHRLYVN
HHHHHHHHCCCCCEEEEEEEECCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHH
TVNKIIQSMKERTVK
HHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3036807; 9384377 [H]