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The map label for this gene is purH [H]

Identifier: 152974118

GI number: 152974118

Start: 318322

End: 319857

Strand: Direct

Name: purH [H]

Synonym: Bcer98_0277

Alternate gene names: 152974118

Gene position: 318322-319857 (Clockwise)

Preceding gene: 152974117

Following gene: 152974119

Centisome position: 7.79

GC content: 38.93

Gene sequence:

>1536_bases
ATGAAAAAGCGTGCATTAGTAAGTGTTTCTAATAAAACAGGAGTAGTAGAATTCGTGAAAGGTTTGCTTGAACAAGGAAT
TGAAGTGATTTCAACAGGTGGCACGAAAAAATTATTAGAGGAAAATGGCTTACAAGTAATGGGGATTTCTGAAGTAACAG
GTTTCCCAGAGATTATGGATGGTCGTGTCAAAACATTACATCCCAATATTCATGGTGGATTACTTGCAGTGCGTGATAAT
GAAGCGCATGTAGCAGAAATGAGCGAATTAGGCATTCAGCTGATTGATTTTGTCGTTGTAAATTTATACCCATTTAAAGA
GACGATTGCTAAGCCTGATGTAACATTTGCTGATGCGATTGAAAATATTGATATCGGTGGTCCAACAATGATTCGTTCAG
CTGCAAAAAATCATAAATTTGTATCAGTAATTGTAGATCCAGCAGACTATGACGTTGTATTAGCTGAATTAAAAGAAAAA
GGTGAGGTTACAGACGAAACAAAGCGTAAATTAGCAGCGAAAGTATTCCGTCATACAGCGGCATATGATGCATTAATCTC
AAACTATTTGACTGAACAAATGGGAGAAGAAAGCCCAGAAATATTAACAGTAACATTCGAGAAAAAGCAAGATTTACGTT
ACGGAGAAAATCCGCATCAAAAAGCAACATTCTATAAAGCACCATTTGCGGTGGCTTCTTCTGTTGCATATGCAGAGCAA
TTGCACGGAAAAGAACTATCTTATAACAACATCAATGACGCAGATGCAGCGCTTAGCATTGTGAAAGAATTTACAGAACC
AGCGGTAGTCGCCGTAAAACATATGAATCCATGCGGAGTTGGTGTTGGTACGGATATCCATGAAGCGTATACACGTGCTT
ATGAGGCGGATCCAGTATCAATCTTCGGCGGCATTATTGCAGCGAACCGTGAAATTGATAAACGTGTGGCAGAGAAATTA
CATGAAATCTTCTTAGAAATTATTATTGCACCTTCATTTTCGAAAGAGGCTTTAGAAGTATTGCAAAGTAAGAAAAACTT
ACGTCTGTTAACGGTAAATATTGAGAAGACAACAAGTGCAAGTAAAAAACTAACTTCTGTTCAAGGTGGACTTCTTGTTC
AAGAAGAAGATACGTTAGCGCTAAATGAAGAGACAATCATAATTCCTACAAAACGTGAACCAACAGAGCAAGAATGGAAC
GACTTAAAATTAGCTTGGAAAGTTGTAAAACATGTAAAATCAAATGCAATTGTACTCGCAAAAGATAATATGACAATCGG
TGTTGGTGCTGGACAAATGAATCGTGTTGGTTCTGCAAAAATTGCAATCTCGCAAGCAGGTAGCAAAGCGCAAGGTAGCG
CCTTAGCATCCGATGCGTTCTTCCCAATGCCAGACACAGTAGAAGAGGCCGCAAAAGCGGGGATTACAGCAATCATTCAA
CCAGGCGGGTCAATCCGTGACGAAGATTCGATTAAAAAAGCGGATGAATATGGGATTACGATGGTGTTCACGGGCGTACG
TCATTTCAAACATTAA

Upstream 100 bases:

>100_bases
AAGAAAATTCAACAAGTGGAACATCGTTTATATGTAAATACGGTAAATAAAATCATTCAATCTATGAAAGAACGAACTGT
GAAATAGGGGTGTAAAAGAA

Downstream 100 bases:

>100_bases
TAGGCATATAAAAGTACATGAAATTTTATCGGCGCAAATTGAAATATATCGACCGTTTCTCATGATATATCGGCGTTTCG
ACATGAGATATCGACATATC

Product: bifunctional phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase

Products: NA

Alternate protein names: Phosphoribosylaminoimidazolecarboxamide formyltransferase; AICAR transformylase; IMP cyclohydrolase; ATIC; IMP synthase; Inosinicase [H]

Number of amino acids: Translated: 511; Mature: 511

Protein sequence:

>511_residues
MKKRALVSVSNKTGVVEFVKGLLEQGIEVISTGGTKKLLEENGLQVMGISEVTGFPEIMDGRVKTLHPNIHGGLLAVRDN
EAHVAEMSELGIQLIDFVVVNLYPFKETIAKPDVTFADAIENIDIGGPTMIRSAAKNHKFVSVIVDPADYDVVLAELKEK
GEVTDETKRKLAAKVFRHTAAYDALISNYLTEQMGEESPEILTVTFEKKQDLRYGENPHQKATFYKAPFAVASSVAYAEQ
LHGKELSYNNINDADAALSIVKEFTEPAVVAVKHMNPCGVGVGTDIHEAYTRAYEADPVSIFGGIIAANREIDKRVAEKL
HEIFLEIIIAPSFSKEALEVLQSKKNLRLLTVNIEKTTSASKKLTSVQGGLLVQEEDTLALNEETIIIPTKREPTEQEWN
DLKLAWKVVKHVKSNAIVLAKDNMTIGVGAGQMNRVGSAKIAISQAGSKAQGSALASDAFFPMPDTVEEAAKAGITAIIQ
PGGSIRDEDSIKKADEYGITMVFTGVRHFKH

Sequences:

>Translated_511_residues
MKKRALVSVSNKTGVVEFVKGLLEQGIEVISTGGTKKLLEENGLQVMGISEVTGFPEIMDGRVKTLHPNIHGGLLAVRDN
EAHVAEMSELGIQLIDFVVVNLYPFKETIAKPDVTFADAIENIDIGGPTMIRSAAKNHKFVSVIVDPADYDVVLAELKEK
GEVTDETKRKLAAKVFRHTAAYDALISNYLTEQMGEESPEILTVTFEKKQDLRYGENPHQKATFYKAPFAVASSVAYAEQ
LHGKELSYNNINDADAALSIVKEFTEPAVVAVKHMNPCGVGVGTDIHEAYTRAYEADPVSIFGGIIAANREIDKRVAEKL
HEIFLEIIIAPSFSKEALEVLQSKKNLRLLTVNIEKTTSASKKLTSVQGGLLVQEEDTLALNEETIIIPTKREPTEQEWN
DLKLAWKVVKHVKSNAIVLAKDNMTIGVGAGQMNRVGSAKIAISQAGSKAQGSALASDAFFPMPDTVEEAAKAGITAIIQ
PGGSIRDEDSIKKADEYGITMVFTGVRHFKH
>Mature_511_residues
MKKRALVSVSNKTGVVEFVKGLLEQGIEVISTGGTKKLLEENGLQVMGISEVTGFPEIMDGRVKTLHPNIHGGLLAVRDN
EAHVAEMSELGIQLIDFVVVNLYPFKETIAKPDVTFADAIENIDIGGPTMIRSAAKNHKFVSVIVDPADYDVVLAELKEK
GEVTDETKRKLAAKVFRHTAAYDALISNYLTEQMGEESPEILTVTFEKKQDLRYGENPHQKATFYKAPFAVASSVAYAEQ
LHGKELSYNNINDADAALSIVKEFTEPAVVAVKHMNPCGVGVGTDIHEAYTRAYEADPVSIFGGIIAANREIDKRVAEKL
HEIFLEIIIAPSFSKEALEVLQSKKNLRLLTVNIEKTTSASKKLTSVQGGLLVQEEDTLALNEETIIIPTKREPTEQEWN
DLKLAWKVVKHVKSNAIVLAKDNMTIGVGAGQMNRVGSAKIAISQAGSKAQGSALASDAFFPMPDTVEEAAKAGITAIIQ
PGGSIRDEDSIKKADEYGITMVFTGVRHFKH

Specific function: De novo purine biosynthesis; ninth step. De novo purine biosynthesis; tenth step. [C]

COG id: COG0138

COG function: function code F; AICAR transformylase/IMP cyclohydrolase PurH (only IMP cyclohydrolase domain in Aful)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purH family [H]

Homologues:

Organism=Homo sapiens, GI20127454, Length=476, Percent_Identity=36.344537815126, Blast_Score=273, Evalue=2e-73,
Organism=Escherichia coli, GI1790439, Length=524, Percent_Identity=52.8625954198473, Blast_Score=543, Evalue=1e-156,
Organism=Caenorhabditis elegans, GI71985564, Length=475, Percent_Identity=34.7368421052632, Blast_Score=265, Evalue=5e-71,
Organism=Caenorhabditis elegans, GI71985574, Length=303, Percent_Identity=27.0627062706271, Blast_Score=95, Evalue=9e-20,
Organism=Saccharomyces cerevisiae, GI6323768, Length=605, Percent_Identity=31.404958677686, Blast_Score=282, Evalue=7e-77,
Organism=Saccharomyces cerevisiae, GI6323056, Length=470, Percent_Identity=35.531914893617, Blast_Score=280, Evalue=3e-76,
Organism=Drosophila melanogaster, GI24649832, Length=472, Percent_Identity=37.2881355932203, Blast_Score=288, Evalue=6e-78,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 640 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002695
- InterPro:   IPR013982
- InterPro:   IPR016193
- InterPro:   IPR011607 [H]

Pfam domain/function: PF01808 AICARFT_IMPCHas; PF02142 MGS [H]

EC number: =2.1.2.3; =3.5.4.10 [H]

Molecular weight: Translated: 55681; Mature: 55681

Theoretical pI: Translated: 5.66; Mature: 5.66

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKRALVSVSNKTGVVEFVKGLLEQGIEVISTGGTKKLLEENGLQVMGISEVTGFPEIMD
CCCCEEEEECCCCCHHHHHHHHHHCCHHEEECCCHHHHHHHCCCEEEEEHHHCCCHHHHC
GRVKTLHPNIHGGLLAVRDNEAHVAEMSELGIQLIDFVVVNLYPFKETIAKPDVTFADAI
CCEEEECCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCHHHHHH
ENIDIGGPTMIRSAAKNHKFVSVIVDPADYDVVLAELKEKGEVTDETKRKLAAKVFRHTA
HCCCCCCHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
AYDALISNYLTEQMGEESPEILTVTFEKKQDLRYGENPHQKATFYKAPFAVASSVAYAEQ
HHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCCCEEEECCHHHHHHHHHHHH
LHGKELSYNNINDADAALSIVKEFTEPAVVAVKHMNPCGVGVGTDIHEAYTRAYEADPVS
HCCCCCCCCCCCHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCHHHHHHHHHCCCCCHH
IFGGIIAANREIDKRVAEKLHEIFLEIIIAPSFSKEALEVLQSKKNLRLLTVNIEKTTSA
HHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCEEEEEEEECCCCCH
SKKLTSVQGGLLVQEEDTLALNEETIIIPTKREPTEQEWNDLKLAWKVVKHVKSNAIVLA
HHHHHHHCCCEEEECCCCEEECCCEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCEEEEE
KDNMTIGVGAGQMNRVGSAKIAISQAGSKAQGSALASDAFFPMPDTVEEAAKAGITAIIQ
ECCEEEEECCCCCCCCCCEEEEEECCCCCCCCCHHCCCCCCCCCHHHHHHHHCCCEEEEC
PGGSIRDEDSIKKADEYGITMVFTGVRHFKH
CCCCCCCCHHHHHHHHCCEEEEEECHHHHCC
>Mature Secondary Structure
MKKRALVSVSNKTGVVEFVKGLLEQGIEVISTGGTKKLLEENGLQVMGISEVTGFPEIMD
CCCCEEEEECCCCCHHHHHHHHHHCCHHEEECCCHHHHHHHCCCEEEEEHHHCCCHHHHC
GRVKTLHPNIHGGLLAVRDNEAHVAEMSELGIQLIDFVVVNLYPFKETIAKPDVTFADAI
CCEEEECCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCHHHHHH
ENIDIGGPTMIRSAAKNHKFVSVIVDPADYDVVLAELKEKGEVTDETKRKLAAKVFRHTA
HCCCCCCHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
AYDALISNYLTEQMGEESPEILTVTFEKKQDLRYGENPHQKATFYKAPFAVASSVAYAEQ
HHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCCCEEEECCHHHHHHHHHHHH
LHGKELSYNNINDADAALSIVKEFTEPAVVAVKHMNPCGVGVGTDIHEAYTRAYEADPVS
HCCCCCCCCCCCHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCHHHHHHHHHCCCCCHH
IFGGIIAANREIDKRVAEKLHEIFLEIIIAPSFSKEALEVLQSKKNLRLLTVNIEKTTSA
HHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCEEEEEEEECCCCCH
SKKLTSVQGGLLVQEEDTLALNEETIIIPTKREPTEQEWNDLKLAWKVVKHVKSNAIVLA
HHHHHHHCCCEEEECCCCEEECCCEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCEEEEE
KDNMTIGVGAGQMNRVGSAKIAISQAGSKAQGSALASDAFFPMPDTVEEAAKAGITAIIQ
ECCEEEEECCCCCCCCCCEEEEEECCCCCCCCCHHCCCCCCCCCHHHHHHHHCCCEEEEC
PGGSIRDEDSIKKADEYGITMVFTGVRHFKH
CCCCCCCCHHHHHHHHCCEEEEEECHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA