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The map label for this gene is purF [H]

Identifier: 152974115

GI number: 152974115

Start: 315167

End: 316582

Strand: Direct

Name: purF [H]

Synonym: Bcer98_0274

Alternate gene names: 152974115

Gene position: 315167-316582 (Clockwise)

Preceding gene: 152974114

Following gene: 152974116

Centisome position: 7.71

GC content: 40.47

Gene sequence:

>1416_bases
ATGCTTGCTGAAATAAAGGGGTTAAATGAAGAATGTGGCATCTTCGGAATTTGGGGGCATGAAAATGCAGCACAAGTTAC
GTACTACGGATTGCATAGTTTACAGCACCGTGGGCAAGAAGGTGCAGGTATTGTGGTGAATAATGGGGAGAAAATTATTG
GTCATAAGGGGTTAGGTTTAGTATCAGAAGTGTTTTCAAGAGGCGAGTTAGAAGGTTTAAATGGAAAATCAGCCATTGGC
CATGTGCGTTATGCAACAGCGGGCGGAAGTGAAGTAGCAAACGTTCAGCCATTACTATTTCGTTTCTCTGATCATAGTAT
GGCATTAGCTCACAATGGGAATTTAATTAATGCCAAAATGCTTCGCCGTGAATTAGAGGCAGAAGGAAGTATCTTTCAAA
CAAGTTCAGATACAGAAGTATTGCTGCATTTAATTAAGCGAAATACGAAAGGGTCATTAATCGAGAGTGTAAAGGATGCG
TTAAATAAAGTAAAAGGTGCATTTGCATATTTGTTACTAACAAGTCATGAAATGATTGTTGCACTAGACCCAAATGGGTT
TCGCCCGCTTTCGATTGGGAAAATGGGGGATGCCTATGTTGTAGCATCTGAAACATGTGCTTTCGATGTAGTTGGTGCGA
CATATATTCGTGATGTTGAACCTGGAGAACTTCTTATCATAAATGATGAAGGGATTCAAGTAGATCGTTTTACAAATGAA
GTAGAGCACGCAATTTGTAGTATGGAATATATTTATTTTGCACGACCAGACTCTAATATTGCCGGCGTCAATGTTCATGC
GGCGCGTAAAAATATGGGGAAACGTTTAGCAATGGAAGCACCTATTGCGGCAGATGTTGTAACAGGGGTACCTGATTCTA
GCATCTCAGCAGCCATTGGTTATGCAGAAGCAACGGGAATTCCATATGAATTAGGATTAATTAAAAATCGTTATGTCGGA
CGTACCTTTATTCAACCATCCCAAGAGCTGCGTGAGCAAGGGGTGAAAATGAAGCTTTCTGCAGTACGCGGTGTAGTTGA
AGGAAAACGTGTTGTCATGATTGATGACTCAATTGTAAGAGGGACAACCAGTAAGCGGATTGTTCGCATGCTTCGTGAAG
CAGGAGCGACAGAAGTGCATGTAAGAATTGCTTCGCCTCCTCTTAAGTATCCATGTTTCTATGGCATTGACATTCAAACG
AGAAAAGAATTAATCGCTGCAAATCACTCTGTAGAGGAAATCTGTCAAATCATCGGTGCAGATTCTCTAACATTTTTAAG
TGAAGATGGATTAGTAGATGCAATTGGACGTCCATATGAAGGGAAATACGGCGGGTTATGCATGGCTTATTTTAATGGAG
ATTATCCGACCGCTCTTTACGATTATGAACAAGAACTTTTAGAAAGTATGAAATAA

Upstream 100 bases:

>100_bases
TCACAAATACAAATGAAGTAACAATTCATAATGAAGAAAATGAAGTACTACTTACAGCAAATGTTGATGAAATGAGAAAG
GCTTGGAAAGGGGCAATCCC

Downstream 100 bases:

>100_bases
AAAAGAAAAGAAGCTTCTACTTCTTTCTATGTAGAAGCTAGCTTCTTTCTGAAAACGGTCGCCCTAGATGGGGAGAAATT
AAAAGACGAGGTGTGAAAAA

Product: amidophosphoribosyltransferase

Products: NA

Alternate protein names: ATase; Glutamine phosphoribosylpyrophosphate amidotransferase; GPATase [H]

Number of amino acids: Translated: 471; Mature: 471

Protein sequence:

>471_residues
MLAEIKGLNEECGIFGIWGHENAAQVTYYGLHSLQHRGQEGAGIVVNNGEKIIGHKGLGLVSEVFSRGELEGLNGKSAIG
HVRYATAGGSEVANVQPLLFRFSDHSMALAHNGNLINAKMLRRELEAEGSIFQTSSDTEVLLHLIKRNTKGSLIESVKDA
LNKVKGAFAYLLLTSHEMIVALDPNGFRPLSIGKMGDAYVVASETCAFDVVGATYIRDVEPGELLIINDEGIQVDRFTNE
VEHAICSMEYIYFARPDSNIAGVNVHAARKNMGKRLAMEAPIAADVVTGVPDSSISAAIGYAEATGIPYELGLIKNRYVG
RTFIQPSQELREQGVKMKLSAVRGVVEGKRVVMIDDSIVRGTTSKRIVRMLREAGATEVHVRIASPPLKYPCFYGIDIQT
RKELIAANHSVEEICQIIGADSLTFLSEDGLVDAIGRPYEGKYGGLCMAYFNGDYPTALYDYEQELLESMK

Sequences:

>Translated_471_residues
MLAEIKGLNEECGIFGIWGHENAAQVTYYGLHSLQHRGQEGAGIVVNNGEKIIGHKGLGLVSEVFSRGELEGLNGKSAIG
HVRYATAGGSEVANVQPLLFRFSDHSMALAHNGNLINAKMLRRELEAEGSIFQTSSDTEVLLHLIKRNTKGSLIESVKDA
LNKVKGAFAYLLLTSHEMIVALDPNGFRPLSIGKMGDAYVVASETCAFDVVGATYIRDVEPGELLIINDEGIQVDRFTNE
VEHAICSMEYIYFARPDSNIAGVNVHAARKNMGKRLAMEAPIAADVVTGVPDSSISAAIGYAEATGIPYELGLIKNRYVG
RTFIQPSQELREQGVKMKLSAVRGVVEGKRVVMIDDSIVRGTTSKRIVRMLREAGATEVHVRIASPPLKYPCFYGIDIQT
RKELIAANHSVEEICQIIGADSLTFLSEDGLVDAIGRPYEGKYGGLCMAYFNGDYPTALYDYEQELLESMK
>Mature_471_residues
MLAEIKGLNEECGIFGIWGHENAAQVTYYGLHSLQHRGQEGAGIVVNNGEKIIGHKGLGLVSEVFSRGELEGLNGKSAIG
HVRYATAGGSEVANVQPLLFRFSDHSMALAHNGNLINAKMLRRELEAEGSIFQTSSDTEVLLHLIKRNTKGSLIESVKDA
LNKVKGAFAYLLLTSHEMIVALDPNGFRPLSIGKMGDAYVVASETCAFDVVGATYIRDVEPGELLIINDEGIQVDRFTNE
VEHAICSMEYIYFARPDSNIAGVNVHAARKNMGKRLAMEAPIAADVVTGVPDSSISAAIGYAEATGIPYELGLIKNRYVG
RTFIQPSQELREQGVKMKLSAVRGVVEGKRVVMIDDSIVRGTTSKRIVRMLREAGATEVHVRIASPPLKYPCFYGIDIQT
RKELIAANHSVEEICQIIGADSLTFLSEDGLVDAIGRPYEGKYGGLCMAYFNGDYPTALYDYEQELLESMK

Specific function: De novo purine biosynthesis; first step. [C]

COG id: COG0034

COG function: function code F; Glutamine phosphoribosylpyrophosphate amidotransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI29570798, Length=463, Percent_Identity=40.3887688984881, Blast_Score=348, Evalue=6e-96,
Organism=Escherichia coli, GI1788651, Length=476, Percent_Identity=40.9663865546218, Blast_Score=320, Evalue=1e-88,
Organism=Escherichia coli, GI1790167, Length=239, Percent_Identity=31.7991631799163, Blast_Score=95, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI17554892, Length=481, Percent_Identity=40.956340956341, Blast_Score=345, Evalue=4e-95,
Organism=Saccharomyces cerevisiae, GI6323958, Length=487, Percent_Identity=37.3716632443532, Blast_Score=267, Evalue=2e-72,
Organism=Drosophila melanogaster, GI28573187, Length=506, Percent_Identity=41.304347826087, Blast_Score=356, Evalue=2e-98,
Organism=Drosophila melanogaster, GI24659598, Length=492, Percent_Identity=39.4308943089431, Blast_Score=352, Evalue=3e-97,
Organism=Drosophila melanogaster, GI24659604, Length=463, Percent_Identity=40.3887688984881, Blast_Score=345, Evalue=5e-95,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005854
- InterPro:   IPR000583
- InterPro:   IPR017932
- InterPro:   IPR000836 [H]

Pfam domain/function: PF00310 GATase_2; PF00156 Pribosyltran [H]

EC number: =2.4.2.14 [H]

Molecular weight: Translated: 51348; Mature: 51348

Theoretical pI: Translated: 5.78; Mature: 5.78

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER ; PS00443 GATASE_TYPE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLAEIKGLNEECGIFGIWGHENAAQVTYYGLHSLQHRGQEGAGIVVNNGEKIIGHKGLGL
CCCCCCCCCCCCCEEEEECCCCCCEEEEEEHHHHHHCCCCCCEEEEECCCEEECCCCCHH
VSEVFSRGELEGLNGKSAIGHVRYATAGGSEVANVQPLLFRFSDHSMALAHNGNLINAKM
HHHHHCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCEEEEEECCCCEEEEECCCEEEHHH
LRRELEAEGSIFQTSSDTEVLLHLIKRNTKGSLIESVKDALNKVKGAFAYLLLTSHEMIV
HHHHHHCCCCEEECCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHEEEEEECCCEEE
ALDPNGFRPLSIGKMGDAYVVASETCAFDVVGATYIRDVEPGELLIINDEGIQVDRFTNE
EECCCCCCEEEECCCCCEEEEEECCCEEEEECHHHEECCCCCCEEEECCCCEEEHHHHHH
VEHAICSMEYIYFARPDSNIAGVNVHAARKNMGKRLAMEAPIAADVVTGVPDSSISAAIG
HHHHHHCEEEEEEECCCCCEEEEEEEHHHHCCCCCEEECCCCHHHHHCCCCCCCCHHHCC
YAEATGIPYELGLIKNRYVGRTFIQPSQELREQGVKMKLSAVRGVVEGKRVVMIDDSIVR
HHHHCCCCEEECCCCCCCCCCCCCCCHHHHHHCCCEEEEHHHHHHHCCCEEEEECCCHHC
GTTSKRIVRMLREAGATEVHVRIASPPLKYPCFYGIDIQTRKELIAANHSVEEICQIIGA
CCHHHHHHHHHHHCCCCEEEEEECCCCCCCCEEECCCHHHHHHHHHCCCCHHHHHHHHCC
DSLTFLSEDGLVDAIGRPYEGKYGGLCMAYFNGDYPTALYDYEQELLESMK
CCEEEEECCCCHHHCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MLAEIKGLNEECGIFGIWGHENAAQVTYYGLHSLQHRGQEGAGIVVNNGEKIIGHKGLGL
CCCCCCCCCCCCCEEEEECCCCCCEEEEEEHHHHHHCCCCCCEEEEECCCEEECCCCCHH
VSEVFSRGELEGLNGKSAIGHVRYATAGGSEVANVQPLLFRFSDHSMALAHNGNLINAKM
HHHHHCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCEEEEEECCCCEEEEECCCEEEHHH
LRRELEAEGSIFQTSSDTEVLLHLIKRNTKGSLIESVKDALNKVKGAFAYLLLTSHEMIV
HHHHHHCCCCEEECCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHEEEEEECCCEEE
ALDPNGFRPLSIGKMGDAYVVASETCAFDVVGATYIRDVEPGELLIINDEGIQVDRFTNE
EECCCCCCEEEECCCCCEEEEEECCCEEEEECHHHEECCCCCCEEEECCCCEEEHHHHHH
VEHAICSMEYIYFARPDSNIAGVNVHAARKNMGKRLAMEAPIAADVVTGVPDSSISAAIG
HHHHHHCEEEEEEECCCCCEEEEEEEHHHHCCCCCEEECCCCHHHHHCCCCCCCCHHHCC
YAEATGIPYELGLIKNRYVGRTFIQPSQELREQGVKMKLSAVRGVVEGKRVVMIDDSIVR
HHHHCCCCEEECCCCCCCCCCCCCCCHHHHHHCCCEEEEHHHHHHHCCCEEEEECCCHHC
GTTSKRIVRMLREAGATEVHVRIASPPLKYPCFYGIDIQTRKELIAANHSVEEICQIIGA
CCHHHHHHHHHHHCCCCEEEEEECCCCCCCCEEECCCHHHHHHHHHCCCCHHHHHHHHCC
DSLTFLSEDGLVDAIGRPYEGKYGGLCMAYFNGDYPTALYDYEQELLESMK
CCEEEEECCCCHHHCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 6411717; 3036807; 9384377; 6411716; 6094545; 3090047; 8197456; 9271502 [H]