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The map label for this gene is dacA [H]

Identifier: 152973863

GI number: 152973863

Start: 17190

End: 18509

Strand: Direct

Name: dacA [H]

Synonym: Bcer98_0009

Alternate gene names: 152973863

Gene position: 17190-18509 (Clockwise)

Preceding gene: 152973862

Following gene: 152973864

Centisome position: 0.42

GC content: 35.15

Gene sequence:

>1320_bases
GTGAAAGGTATGTTTTGCAAAAGATTCATTGCAATGGTAACAGTGCTGACATTATTTTTTAGCGCGTTCGTTACATATAG
CAGTGTATCAGCAGAAACAAGTTCTGCTTTAAATATTGAAGCAGGCGCAGCAATTTTAGTAGAAGCAGATTCTGGGAAAA
TTTTATATCAAAAAAATGCAGATGAATTGTTAGCAATTGCAAGTATGACGAAAATGATGAGTGAATATTTAGTTCATAAA
GCGGTGGATACAGGGAAACTTAAATGGGATCAAAAAATAAAAGTTTCTGAATATGCACATAAAATTTCACAAGACCGCTC
ATTATCAAATGTTCCGCTAGAAAATGGTGGTTCTTACACAGTGAAAGAATTGTATGAAGCAATGGCAATTTACTCCGCAA
ACGGCGCAACAATTGCTTTAGCTGAGGCGGTTGCAGGTAAAGAAGCAGACTTCGTAAAAATGATGAATGATCAAGCAAAA
GAGTTTGGATTGAAAAATTATAAATTTGTAAACTCTACAGGTTTAACAAACAAAGATTTAAAAGGACAACATCCTGAAGG
AACAACACCGGATGAGGAAAATAAAATGTCGGCACGAGATGTTGCCATTTTAGCACAGCATTTAATTCAAGATTTTCCAA
AGGTGTTAGATACGGCGAAAATTCCGAAGAAGGTATTCCAAGAAGGTGGAAAATATCCTGTTAAGATGGATAACTGGAAC
TGGATGTTAAAAGGCTTAATTAAACAATATGAAGGTGTAGATGGTTTAAAAACAGGTTCTACTCCAGAAGCTGGTGATTG
CTTTACAGGTACTGCTGAAAGAAATGGAATGCGTTTAATTTCTGTTGTTATTAAAACAAATTCCCATACAGCGCGCTTTG
ATGAAACAAAGAAACTTTTTGATTACGGATTTACCAATTTTGATGTAAAAAAAGTATATCCAAAAGGATCATCAGTAAAG
GGATATGAAACGGTACGAGTTGAAAATGCAAAAGATAAAGATGTTGCAGTTGAGACAAAACAAGCGATTACACTTCCAAT
GCCAAAAGGAAGTAAAGATGTTTACAAAACAGAGTTTAAAGAGGTAGGAAAAGAAAAAGAAGCACCTATTAAAAAAGGAA
CAACATTAGGACAAATGGTTGTATCACCTAAGGATGCGAGTGATCCAGGATTCTTATCTGGGAAATCATTGCATGTTGAT
TTAGTAACAAAATCAGAAGTAGAACAAGCAAACTGGTTTGTACGCTCTATGCGTGGTATAGGATCCTTCTTTAGCGACAT
ATGGAATAGTGCTGTTGATACAGTAAAAGGTTGGTTTTAA

Upstream 100 bases:

>100_bases
ATATATAAATAGACAGAGGTTTGATACCTCTGTCTATTTTTTTTTGATTATGTTAGAATAACGGTTATGTGAGTACAATG
AGATATTGGGGGTAACTGAA

Downstream 100 bases:

>100_bases
AAGCTCCTCATAGTAGGAGCTTTTTTTATTTTTGATTTTTCATACACATTTTATGAAAAAGTAGTGGACAAGTCTCTAAT
AGTTAGTGATAGAATGTAAG

Product: Serine-type D-Ala-D-Ala carboxypeptidase

Products: NA

Alternate protein names: CPase; DD-carboxypeptidase; DD-peptidase; Penicillin-binding protein 5; PBP-5 [H]

Number of amino acids: Translated: 439; Mature: 439

Protein sequence:

>439_residues
MKGMFCKRFIAMVTVLTLFFSAFVTYSSVSAETSSALNIEAGAAILVEADSGKILYQKNADELLAIASMTKMMSEYLVHK
AVDTGKLKWDQKIKVSEYAHKISQDRSLSNVPLENGGSYTVKELYEAMAIYSANGATIALAEAVAGKEADFVKMMNDQAK
EFGLKNYKFVNSTGLTNKDLKGQHPEGTTPDEENKMSARDVAILAQHLIQDFPKVLDTAKIPKKVFQEGGKYPVKMDNWN
WMLKGLIKQYEGVDGLKTGSTPEAGDCFTGTAERNGMRLISVVIKTNSHTARFDETKKLFDYGFTNFDVKKVYPKGSSVK
GYETVRVENAKDKDVAVETKQAITLPMPKGSKDVYKTEFKEVGKEKEAPIKKGTTLGQMVVSPKDASDPGFLSGKSLHVD
LVTKSEVEQANWFVRSMRGIGSFFSDIWNSAVDTVKGWF

Sequences:

>Translated_439_residues
MKGMFCKRFIAMVTVLTLFFSAFVTYSSVSAETSSALNIEAGAAILVEADSGKILYQKNADELLAIASMTKMMSEYLVHK
AVDTGKLKWDQKIKVSEYAHKISQDRSLSNVPLENGGSYTVKELYEAMAIYSANGATIALAEAVAGKEADFVKMMNDQAK
EFGLKNYKFVNSTGLTNKDLKGQHPEGTTPDEENKMSARDVAILAQHLIQDFPKVLDTAKIPKKVFQEGGKYPVKMDNWN
WMLKGLIKQYEGVDGLKTGSTPEAGDCFTGTAERNGMRLISVVIKTNSHTARFDETKKLFDYGFTNFDVKKVYPKGSSVK
GYETVRVENAKDKDVAVETKQAITLPMPKGSKDVYKTEFKEVGKEKEAPIKKGTTLGQMVVSPKDASDPGFLSGKSLHVD
LVTKSEVEQANWFVRSMRGIGSFFSDIWNSAVDTVKGWF
>Mature_439_residues
MKGMFCKRFIAMVTVLTLFFSAFVTYSSVSAETSSALNIEAGAAILVEADSGKILYQKNADELLAIASMTKMMSEYLVHK
AVDTGKLKWDQKIKVSEYAHKISQDRSLSNVPLENGGSYTVKELYEAMAIYSANGATIALAEAVAGKEADFVKMMNDQAK
EFGLKNYKFVNSTGLTNKDLKGQHPEGTTPDEENKMSARDVAILAQHLIQDFPKVLDTAKIPKKVFQEGGKYPVKMDNWN
WMLKGLIKQYEGVDGLKTGSTPEAGDCFTGTAERNGMRLISVVIKTNSHTARFDETKKLFDYGFTNFDVKKVYPKGSSVK
GYETVRVENAKDKDVAVETKQAITLPMPKGSKDVYKTEFKEVGKEKEAPIKKGTTLGQMVVSPKDASDPGFLSGKSLHVD
LVTKSEVEQANWFVRSMRGIGSFFSDIWNSAVDTVKGWF

Specific function: Removes C-terminal D-alanyl residues from sugar-peptide cell wall precursors [H]

COG id: COG1686

COG function: function code M; D-alanyl-D-alanine carboxypeptidase

Gene ontology:

Cell location: Secreted, cell wall [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S11 family [H]

Homologues:

Organism=Escherichia coli, GI1787062, Length=425, Percent_Identity=30.3529411764706, Blast_Score=155, Evalue=5e-39,
Organism=Escherichia coli, GI1786851, Length=421, Percent_Identity=27.0783847980998, Blast_Score=130, Evalue=1e-31,
Organism=Escherichia coli, GI87082022, Length=386, Percent_Identity=28.4974093264249, Blast_Score=129, Evalue=3e-31,
Organism=Escherichia coli, GI87082054, Length=298, Percent_Identity=25.503355704698, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR015956
- InterPro:   IPR018044
- InterPro:   IPR012907
- InterPro:   IPR001967 [H]

Pfam domain/function: PF07943 PBP5_C; PF00768 Peptidase_S11 [H]

EC number: =3.4.16.4 [H]

Molecular weight: Translated: 48503; Mature: 48503

Theoretical pI: Translated: 9.16; Mature: 9.16

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKGMFCKRFIAMVTVLTLFFSAFVTYSSVSAETSSALNIEAGAAILVEADSGKILYQKNA
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCEEEEEECCCCCEEEECCH
DELLAIASMTKMMSEYLVHKAVDTGKLKWDQKIKVSEYAHKISQDRSLSNVPLENGGSYT
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCEE
VKELYEAMAIYSANGATIALAEAVAGKEADFVKMMNDQAKEFGLKNYKFVNSTGLTNKDL
HHHHHHHHHHCCCCCCEEEEHHHHCCCCHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCC
KGQHPEGTTPDEENKMSARDVAILAQHLIQDFPKVLDTAKIPKKVFQEGGKYPVKMDNWN
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCHH
WMLKGLIKQYEGVDGLKTGSTPEAGDCFTGTAERNGMRLISVVIKTNSHTARFDETKKLF
HHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEEECCCCCCHHHHHHHHH
DYGFTNFDVKKVYPKGSSVKGYETVRVENAKDKDVAVETKQAITLPMPKGSKDVYKTEFK
HCCCCCCEEEEECCCCCCCCCEEEEEECCCCCCCCEEECCCEEEECCCCCCCHHHHHHHH
EVGKEKEAPIKKGTTLGQMVVSPKDASDPGFLSGKSLHVDLVTKSEVEQANWFVRSMRGI
HHCCHHCCCCCCCCCHHHEEECCCCCCCCCCCCCCEEEEEEECHHHHHHHHHHHHHHHHH
GSFFSDIWNSAVDTVKGWF
HHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MKGMFCKRFIAMVTVLTLFFSAFVTYSSVSAETSSALNIEAGAAILVEADSGKILYQKNA
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCEEEEEECCCCCEEEECCH
DELLAIASMTKMMSEYLVHKAVDTGKLKWDQKIKVSEYAHKISQDRSLSNVPLENGGSYT
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCEE
VKELYEAMAIYSANGATIALAEAVAGKEADFVKMMNDQAKEFGLKNYKFVNSTGLTNKDL
HHHHHHHHHHCCCCCCEEEEHHHHCCCCHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCC
KGQHPEGTTPDEENKMSARDVAILAQHLIQDFPKVLDTAKIPKKVFQEGGKYPVKMDNWN
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCHH
WMLKGLIKQYEGVDGLKTGSTPEAGDCFTGTAERNGMRLISVVIKTNSHTARFDETKKLF
HHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEEECCCCCCHHHHHHHHH
DYGFTNFDVKKVYPKGSSVKGYETVRVENAKDKDVAVETKQAITLPMPKGSKDVYKTEFK
HCCCCCCEEEEECCCCCCCCCEEEEEECCCCCCCCEEECCCEEEECCCCCCCHHHHHHHH
EVGKEKEAPIKKGTTLGQMVVSPKDASDPGFLSGKSLHVDLVTKSEVEQANWFVRSMRGI
HHCCHHCCCCCCCCCHHHEEECCCCCCCCCCCCCCEEEEEEECHHHHHHHHHHHHHHHHH
GSFFSDIWNSAVDTVKGWF
HHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8370539; 6780559; 111240 [H]