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The map label for this gene is hmpA [H]

Identifier: 152971408

GI number: 152971408

Start: 3161621

End: 3162811

Strand: Direct

Name: hmpA [H]

Synonym: KPN_02877

Alternate gene names: 152971408

Gene position: 3161621-3162811 (Clockwise)

Preceding gene: 152971406

Following gene: 152971414

Centisome position: 59.48

GC content: 57.26

Gene sequence:

>1191_bases
ATGCTTGACGCTCAAACCATCGCCACGGTAAAAGCCACCATTCCCCTGCTGGTAGAGACCGGCCCGAAATTAACCGCCCA
TTTCTACGATCGTATGTTCGCGCATAATCCGGAGCTCAAAGAGATCTTCAACATGAGCAACCAGCGCAACGGCGATCAGC
GTGAAGCGTTATTCAACGCTATTGCCGCCTACGCCAGCAATATCGATAACCTGCCTGCCCTGCTGCCGGCGGTGGAAAAG
ATCGCCCAGAAACACACCAGCTTCCAGATCAAACCGGAGCAGTACAATATTGTTGGCAGCCATCTGCTGGCGACCCTCGA
TGAAATGTTCAGTCCGGGCCAGGAAGTGCTCGATGCCTGGGGTAAAGCCTACGGCGTGCTGGCGAACGTCTTTATCGGCC
GTGAAGCCGAGATCTATCAGCAAAACGCCAGTAAAACCGGCGGCTGGGAAGGGACCCGCGCGTTTCGCATCGTGAAGAAA
ACCCCGCGCAGCCAGCTTATCACCAGCTTTGAGCTCGAACCGGTTGATGGCCAGCCGGTCGCCGATTACCAGCCTGGCCA
GTACCTGGCCATCTGGCTGAAACCGGAAGGCTTCGAATATCAGGAAATCCGCCAGTATTCCCTGACCCGCAAAGCGGACG
GCAAAGGCTACCGCATTGCGGTTAAACGCGAGGAAGGCGGTCAGGTTTCCAGCTGGCTGCATAACCATGCCAGCGAAGGC
GATGTGGTCTATCTGGCCGCCCCGGCGGGAGATTTCTTCCTTAACGTTAAGCCGCAGACGCCAGTCACCCTGCTCTCCGG
CGGCGTCGGGCAAACGCCGATGCTGGCGATGCTGGATGCGCTGGCGAAGAGCGGTCATCAGGGTCAGGTGAACTGGTTCC
ATGCGGCGGAAAATGGCGACGTTCACGCCTTTGCCGATGAAGTGAAGGCGCTCGGCGCGGCGCTGCCGGCGTTTACCTCT
CACGTCTGGTATCGCACCCCGACTGAGGCCGACCGTCAGGCTGGACGTTTTGATAGCGAAGGTTTGATGGATTTAGCAGC
AGTGGCGGACAATATTCGCGACCCGCAGATGCAGTATTATCTCTGCGGCCCGGTAGCGTTTATGCAGTTTGCAGCGAAGC
AGCTGGTCGAATTAGGGGTCAACAAAGACAACATTCATTACGAATGCTTCGGCCCGCATAAGGTGCTGTAA

Upstream 100 bases:

>100_bases
GGATCGGGGGAATGTGTTAAAGAACCATTTACAACGCAGGGTTATTTCTTTAAGATGCATTTAAAATGCATCATTAAGAT
AACACATAAGGAAGCACGCT

Downstream 100 bases:

>100_bases
GCCCCGACGACGCGGGGATCTGTAGGCCGGGTAAGCGCAGCGCCACCCGGCAATACTGCAGCGAGCGGCGAGCGTTAAAT
CGCCGCGTCGTCTTCTTCGC

Product: nitric oxide dioxygenase

Products: NA

Alternate protein names: Flavohemoglobin; Hemoglobin-like protein; Nitric oxide dioxygenase; NO oxygenase; NOD [H]

Number of amino acids: Translated: 396; Mature: 396

Protein sequence:

>396_residues
MLDAQTIATVKATIPLLVETGPKLTAHFYDRMFAHNPELKEIFNMSNQRNGDQREALFNAIAAYASNIDNLPALLPAVEK
IAQKHTSFQIKPEQYNIVGSHLLATLDEMFSPGQEVLDAWGKAYGVLANVFIGREAEIYQQNASKTGGWEGTRAFRIVKK
TPRSQLITSFELEPVDGQPVADYQPGQYLAIWLKPEGFEYQEIRQYSLTRKADGKGYRIAVKREEGGQVSSWLHNHASEG
DVVYLAAPAGDFFLNVKPQTPVTLLSGGVGQTPMLAMLDALAKSGHQGQVNWFHAAENGDVHAFADEVKALGAALPAFTS
HVWYRTPTEADRQAGRFDSEGLMDLAAVADNIRDPQMQYYLCGPVAFMQFAAKQLVELGVNKDNIHYECFGPHKVL

Sequences:

>Translated_396_residues
MLDAQTIATVKATIPLLVETGPKLTAHFYDRMFAHNPELKEIFNMSNQRNGDQREALFNAIAAYASNIDNLPALLPAVEK
IAQKHTSFQIKPEQYNIVGSHLLATLDEMFSPGQEVLDAWGKAYGVLANVFIGREAEIYQQNASKTGGWEGTRAFRIVKK
TPRSQLITSFELEPVDGQPVADYQPGQYLAIWLKPEGFEYQEIRQYSLTRKADGKGYRIAVKREEGGQVSSWLHNHASEG
DVVYLAAPAGDFFLNVKPQTPVTLLSGGVGQTPMLAMLDALAKSGHQGQVNWFHAAENGDVHAFADEVKALGAALPAFTS
HVWYRTPTEADRQAGRFDSEGLMDLAAVADNIRDPQMQYYLCGPVAFMQFAAKQLVELGVNKDNIHYECFGPHKVL
>Mature_396_residues
MLDAQTIATVKATIPLLVETGPKLTAHFYDRMFAHNPELKEIFNMSNQRNGDQREALFNAIAAYASNIDNLPALLPAVEK
IAQKHTSFQIKPEQYNIVGSHLLATLDEMFSPGQEVLDAWGKAYGVLANVFIGREAEIYQQNASKTGGWEGTRAFRIVKK
TPRSQLITSFELEPVDGQPVADYQPGQYLAIWLKPEGFEYQEIRQYSLTRKADGKGYRIAVKREEGGQVSSWLHNHASEG
DVVYLAAPAGDFFLNVKPQTPVTLLSGGVGQTPMLAMLDALAKSGHQGQVNWFHAAENGDVHAFADEVKALGAALPAFTS
HVWYRTPTEADRQAGRFDSEGLMDLAAVADNIRDPQMQYYLCGPVAFMQFAAKQLVELGVNKDNIHYECFGPHKVL

Specific function: Is involved in NO detoxification in an aerobic process, termed nitric oxide dioxygenase (NOD) reaction that utilizes O(2) and NAD(P)H to convert NO to nitrate, which protects the bacterium from various noxious nitrogen compounds. Therefore, plays a centra

COG id: COG1018

COG function: function code C; Flavodoxin reductases (ferredoxin-NADPH reductases) family 1

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FAD-binding FR-type domain [H]

Homologues:

Organism=Escherichia coli, GI1788903, Length=396, Percent_Identity=84.3434343434343, Blast_Score=704, Evalue=0.0,
Organism=Escherichia coli, GI1787658, Length=239, Percent_Identity=26.3598326359833, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1788104, Length=234, Percent_Identity=24.7863247863248, Blast_Score=64, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6321673, Length=411, Percent_Identity=34.7931873479319, Blast_Score=213, Evalue=4e-56,

Paralogues:

None

Copy number: 100 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017927
- InterPro:   IPR012292
- InterPro:   IPR009050
- InterPro:   IPR000971
- InterPro:   IPR008333
- InterPro:   IPR001433
- InterPro:   IPR001221
- InterPro:   IPR017938 [H]

Pfam domain/function: PF00970 FAD_binding_6; PF00042 Globin; PF00175 NAD_binding_1 [H]

EC number: =1.14.12.17 [H]

Molecular weight: Translated: 43815; Mature: 43815

Theoretical pI: Translated: 5.93; Mature: 5.93

Prosite motif: PS01033 GLOBIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLDAQTIATVKATIPLLVETGPKLTAHFYDRMFAHNPELKEIFNMSNQRNGDQREALFNA
CCCCHHHHHHHHEEEEEEECCCCHHHHHHHHHHHCCCCHHHHHCCCCCCCCHHHHHHHHH
IAAYASNIDNLPALLPAVEKIAQKHTSFQIKPEQYNIVGSHLLATLDEMFSPGQEVLDAW
HHHHHHCCCCCHHHHHHHHHHHHHHCCEEECCHHHHHHHHHHHHHHHHHHCCHHHHHHHH
GKAYGVLANVFIGREAEIYQQNASKTGGWEGTRAFRIVKKTPRSQLITSFELEPVDGQPV
HHHHHHHHHHHCCCCHHHHHHCCCCCCCCCCHHEEEEEHHCCHHHHHEEEEECCCCCCCC
ADYQPGQYLAIWLKPEGFEYQEIRQYSLTRKADGKGYRIAVKREEGGQVSSWLHNHASEG
CCCCCCCEEEEEECCCCCCHHHHHHHHHHHCCCCCCEEEEEEECCCCHHHHHHHHCCCCC
DVVYLAAPAGDFFLNVKPQTPVTLLSGGVGQTPMLAMLDALAKSGHQGQVNWFHAAENGD
CEEEEEECCCCEEEEECCCCCEEEECCCCCCCHHHHHHHHHHHCCCCCCEEEEEECCCCC
VHAFADEVKALGAALPAFTSHVWYRTPTEADRQAGRFDSEGLMDLAAVADNIRDPQMQYY
CHHHHHHHHHHHHHHHHHHHCCEECCCCHHHHHCCCCCCCHHHHHHHHHHCCCCCCEEEE
LCGPVAFMQFAAKQLVELGVNKDNIHYECFGPHKVL
EECHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCC
>Mature Secondary Structure
MLDAQTIATVKATIPLLVETGPKLTAHFYDRMFAHNPELKEIFNMSNQRNGDQREALFNA
CCCCHHHHHHHHEEEEEEECCCCHHHHHHHHHHHCCCCHHHHHCCCCCCCCHHHHHHHHH
IAAYASNIDNLPALLPAVEKIAQKHTSFQIKPEQYNIVGSHLLATLDEMFSPGQEVLDAW
HHHHHHCCCCCHHHHHHHHHHHHHHCCEEECCHHHHHHHHHHHHHHHHHHCCHHHHHHHH
GKAYGVLANVFIGREAEIYQQNASKTGGWEGTRAFRIVKKTPRSQLITSFELEPVDGQPV
HHHHHHHHHHHCCCCHHHHHHCCCCCCCCCCHHEEEEEHHCCHHHHHEEEEECCCCCCCC
ADYQPGQYLAIWLKPEGFEYQEIRQYSLTRKADGKGYRIAVKREEGGQVSSWLHNHASEG
CCCCCCCEEEEEECCCCCCHHHHHHHHHHHCCCCCCEEEEEEECCCCHHHHHHHHCCCCC
DVVYLAAPAGDFFLNVKPQTPVTLLSGGVGQTPMLAMLDALAKSGHQGQVNWFHAAENGD
CEEEEEECCCCEEEEECCCCCEEEECCCCCCCHHHHHHHHHHHCCCCCCEEEEEECCCCC
VHAFADEVKALGAALPAFTSHVWYRTPTEADRQAGRFDSEGLMDLAAVADNIRDPQMQYY
CHHHHHHHHHHHHHHHHHHHCCEECCCCHHHHHCCCCCCCHHHHHHHHHHCCCCCCEEEE
LCGPVAFMQFAAKQLVELGVNKDNIHYECFGPHKVL
EECHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]