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The map label for this gene is yfjR [H]

Identifier: 152970239

GI number: 152970239

Start: 1865722

End: 1866555

Strand: Direct

Name: yfjR [H]

Synonym: KPN_01687

Alternate gene names: 152970239

Gene position: 1865722-1866555 (Clockwise)

Preceding gene: 152970237

Following gene: 152970254

Centisome position: 35.1

GC content: 63.67

Gene sequence:

>834_bases
ATGGGACACGCTTTTGCCAGTAACCTGCTGAAAAACGGCTTTACCGTTGCCGGCTGGAACCGTTCGCCAGCCCGCGGCGA
AGATTTACAGGCGCATGGGTTATCTCTGCACGCCACGCCGCAGCAGGCGGTGGCCGATGCCGAGGTGATCATCAGTATGC
TCGCCGACGGTGAGGCCACCCTCGAGGTACTGGCGCAAATCGCGCCCGCCTGTCAGCCGCAGGCGATCTATTGCCAGATG
GGCACCATTGGCCTGCCGGAGACCCGGCAGGCCATCGCTATGTTGCGGGAACTTCAGCCAGCGATGACCTATATCGATGC
CCCGGTGTCGGGGACCAAAGCCCCCGCGGAGAAGGCACAGATCCTGGTGCTCGCCAGCGGAGATAGGGAAAAGGGCGCCG
CTGCCGAACCGGTGTTTGCGGCTATCTCCCGCGGGACGCAGTGGTTTGGCGAGGCGGGCAACAGCCAGAAAATGAAGCTG
GTCCTCAATGCCTGGCTGATTTCGATGATGCAGGGCATCGCGGAGAGCGCCCAGCTGGCGAAGACGCTGGGCTTCACCCC
CGACCAGCTGTGGAGCGCCCTGGAAGGCGGCCCGCTGGCGGCGCCCTATGTCAAAGTGAAACTGGACGCCATCGCCAGCG
AGCAGTATACCCCGCAGATGCAGCTGGCGCATGCCCTGAAAGATGCCCGGCTGGCGCTTTCCCTGGCCGAGCCGCACACC
ATGCCGGGGCTGGAAAACATCGCCGAACTGTGGCAGCAGGCGGCGGACGCGGGATATGCCGGAGAGGATTTGTCTGCGGT
ATATCAGTGGCTGTCGCCGTCGTCAAAAGCGTAG

Upstream 100 bases:

>100_bases
GCCAGAGATGCGATAGTAGAGGTCCGCTTTTATTAAGACGCGAATCAGGACAGACAAGATGAGTACATTACCAAAGGTCG
CGGTGCTAGGACTCGGCGCC

Downstream 100 bases:

>100_bases
TTTCCTACCCACGCCTGGAAAGAAGTGGCCATGGTGGCCACTTATCCTGGCGGATGGGGCATCAATTCGAATCTACCGTA
CGTTAGCTTAAGCTATCAAT

Product: putative dehydrogenase

Products: 2-methyl-3-oxopropanoate; NADH; H+

Alternate protein names: NA

Number of amino acids: Translated: 277; Mature: 276

Protein sequence:

>277_residues
MGHAFASNLLKNGFTVAGWNRSPARGEDLQAHGLSLHATPQQAVADAEVIISMLADGEATLEVLAQIAPACQPQAIYCQM
GTIGLPETRQAIAMLRELQPAMTYIDAPVSGTKAPAEKAQILVLASGDREKGAAAEPVFAAISRGTQWFGEAGNSQKMKL
VLNAWLISMMQGIAESAQLAKTLGFTPDQLWSALEGGPLAAPYVKVKLDAIASEQYTPQMQLAHALKDARLALSLAEPHT
MPGLENIAELWQQAADAGYAGEDLSAVYQWLSPSSKA

Sequences:

>Translated_277_residues
MGHAFASNLLKNGFTVAGWNRSPARGEDLQAHGLSLHATPQQAVADAEVIISMLADGEATLEVLAQIAPACQPQAIYCQM
GTIGLPETRQAIAMLRELQPAMTYIDAPVSGTKAPAEKAQILVLASGDREKGAAAEPVFAAISRGTQWFGEAGNSQKMKL
VLNAWLISMMQGIAESAQLAKTLGFTPDQLWSALEGGPLAAPYVKVKLDAIASEQYTPQMQLAHALKDARLALSLAEPHT
MPGLENIAELWQQAADAGYAGEDLSAVYQWLSPSSKA
>Mature_276_residues
GHAFASNLLKNGFTVAGWNRSPARGEDLQAHGLSLHATPQQAVADAEVIISMLADGEATLEVLAQIAPACQPQAIYCQMG
TIGLPETRQAIAMLRELQPAMTYIDAPVSGTKAPAEKAQILVLASGDREKGAAAEPVFAAISRGTQWFGEAGNSQKMKLV
LNAWLISMMQGIAESAQLAKTLGFTPDQLWSALEGGPLAAPYVKVKLDAIASEQYTPQMQLAHALKDARLALSLAEPHTM
PGLENIAELWQQAADAGYAGEDLSAVYQWLSPSSKA

Specific function: Unknown

COG id: COG2084

COG function: function code I; 3-hydroxyisobutyrate dehydrogenase and related beta-hydroxyacid dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 3-hydroxyisobutyrate dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI40556376, Length=277, Percent_Identity=28.5198555956679, Blast_Score=114, Evalue=7e-26,
Organism=Homo sapiens, GI23308751, Length=288, Percent_Identity=25.3472222222222, Blast_Score=67, Evalue=2e-11,
Organism=Escherichia coli, GI1790315, Length=281, Percent_Identity=28.1138790035587, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1786719, Length=277, Percent_Identity=25.2707581227437, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI145693186, Length=283, Percent_Identity=24.0282685512367, Blast_Score=66, Evalue=3e-12,
Organism=Drosophila melanogaster, GI28574115, Length=274, Percent_Identity=25.9124087591241, Blast_Score=103, Evalue=2e-22,
Organism=Drosophila melanogaster, GI24655230, Length=288, Percent_Identity=24.6527777777778, Blast_Score=64, Evalue=7e-11,
Organism=Drosophila melanogaster, GI19922568, Length=288, Percent_Identity=24.6527777777778, Blast_Score=64, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015815
- InterPro:   IPR008927
- InterPro:   IPR006115
- InterPro:   IPR013328
- InterPro:   IPR016040 [H]

Pfam domain/function: PF03446 NAD_binding_2 [H]

EC number: 1.1.1.31

Molecular weight: Translated: 29348; Mature: 29217

Theoretical pI: Translated: 4.79; Mature: 4.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGHAFASNLLKNGFTVAGWNRSPARGEDLQAHGLSLHATPQQAVADAEVIISMLADGEAT
CCHHHHHHHHHCCCEEECCCCCCCCCCCCHHCCEEEECCHHHHHHHHHHHHHHHCCCHHH
LEVLAQIAPACQPQAIYCQMGTIGLPETRQAIAMLRELQPAMTYIDAPVSGTKAPAEKAQ
HHHHHHHHCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCE
ILVLASGDREKGAAAEPVFAAISRGTQWFGEAGNSQKMKLVLNAWLISMMQGIAESAQLA
EEEEECCCCCCCCCCCHHHHHHHHCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
KTLGFTPDQLWSALEGGPLAAPYVKVKLDAIASEQYTPQMQLAHALKDARLALSLAEPHT
HHHCCCHHHHHHHHCCCCCCCCEEEEEHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCC
MPGLENIAELWQQAADAGYAGEDLSAVYQWLSPSSKA
CCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
GHAFASNLLKNGFTVAGWNRSPARGEDLQAHGLSLHATPQQAVADAEVIISMLADGEAT
CHHHHHHHHHCCCEEECCCCCCCCCCCCHHCCEEEECCHHHHHHHHHHHHHHHCCCHHH
LEVLAQIAPACQPQAIYCQMGTIGLPETRQAIAMLRELQPAMTYIDAPVSGTKAPAEKAQ
HHHHHHHHCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCE
ILVLASGDREKGAAAEPVFAAISRGTQWFGEAGNSQKMKLVLNAWLISMMQGIAESAQLA
EEEEECCCCCCCCCCCHHHHHHHHCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
KTLGFTPDQLWSALEGGPLAAPYVKVKLDAIASEQYTPQMQLAHALKDARLALSLAEPHT
HHHCCCHHHHHHHHCCCCCCCCEEEEEHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCC
MPGLENIAELWQQAADAGYAGEDLSAVYQWLSPSSKA
CCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 3-hydroxy-2-methylpropanoate; NAD+

Specific reaction: 3-hydroxy-2-methylpropanoate + NAD+ = 2-methyl-3-oxopropanoate + NADH + H+

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969503; 9384377 [H]