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The map label for this gene is cynR [H]

Identifier: 152970237

GI number: 152970237

Start: 1864684

End: 1865568

Strand: Direct

Name: cynR [H]

Synonym: KPN_01685

Alternate gene names: 152970237

Gene position: 1864684-1865568 (Clockwise)

Preceding gene: 152970236

Following gene: 152970239

Centisome position: 35.08

GC content: 66.21

Gene sequence:

>885_bases
ATGCGACTGGATCTGGCCGATCTGCGGCTATTTATCGCCATCGTGGATACCGGCAGTATCACCGGCGGAGCGGCGAATGC
CCATCTGGCCCTGGCGTCCGCCAGTGAGAGGCTGCGCAAGATGGAGGCCGAGATCGGCGTGCCGCTGTTGCACCGTCATG
CCCGCGGGGTGACGATGACAGAGGCCGGGGAGGTACTGGCGCGCCATGCCCGCCCGCTTCTTGCGCAGCAGCAGCGTCTG
CGCCAGGCGATGCACGCCTTCGCCTGCGGTCAGCGGGGGACGCTGCGCCTGTTCGCCAACACCTCGGCGATGTCGGCCTT
TCTGCCGGGGAAGCTCGCCGCCTGGATGGCAGCGCACCCGGCGGTGCAGATCGAAACCGAAGAACGCACCAGCGCGGATA
TCGTCAGCTGTATCCTGGCGGGCGTGGCGCAGGCCGGAGTGGTGTCCGATGCGGTCGACCCCGGGCCGCTGCGCCTCGAT
CCCGTCGCTGACGACCCGCTGGTGCTGATCGTGCCGCCAGCTCGCCCTCTGGCCGAAAAAGAGGAAGTGGCGTTTGCGGC
GGTGCTCAACGAGCCGTTGGTCGCCCTCTATCAGACCAGTGCGCTACAGCAGCATATCGAACAGCACGCAGCTGAACTCG
GCCAGGCGCTAAACGTTCGGGTCAGGATGAGCCATTTTGCAGGGCTTTGCGAGATGGTGGCGCACGGCGCCGGCGGGGCG
ATCCTGCCGCGCGTTATTGCCGAGCGTTACCGGCAGCGCTATTCGTTTGCCGTCATCGGGTTACAGGACCGCTGGGCGCA
GCGCCGTTTGTGCCTGTGCTATCAGGATGACGCCAGCCTGTCGCCAGCGATGCGCCGCTTACTGGAATGGCTTCGTCAGC
CTTGA

Upstream 100 bases:

>100_bases
TTTCAGGGTAGAAAGGGGCATTGAAACAGCTGGTCGTGAGGGCCACAATGCGGTATGTTGGCCATAGACTACGGAAAAAC
CGAAGGGAAAGGAGGGCCTG

Downstream 100 bases:

>100_bases
GAACATCAGTGACAGTGTCACTTTTCATGCCCGTTATTGCTACTGACATCCGTGCCAGAGATGCGATAGTAGAGGTCCGC
TTTTATTAAGACGCGAATCA

Product: transcriptional regulator LysR

Products: NA

Alternate protein names: Cyn operon transcriptional activator [H]

Number of amino acids: Translated: 294; Mature: 294

Protein sequence:

>294_residues
MRLDLADLRLFIAIVDTGSITGGAANAHLALASASERLRKMEAEIGVPLLHRHARGVTMTEAGEVLARHARPLLAQQQRL
RQAMHAFACGQRGTLRLFANTSAMSAFLPGKLAAWMAAHPAVQIETEERTSADIVSCILAGVAQAGVVSDAVDPGPLRLD
PVADDPLVLIVPPARPLAEKEEVAFAAVLNEPLVALYQTSALQQHIEQHAAELGQALNVRVRMSHFAGLCEMVAHGAGGA
ILPRVIAERYRQRYSFAVIGLQDRWAQRRLCLCYQDDASLSPAMRRLLEWLRQP

Sequences:

>Translated_294_residues
MRLDLADLRLFIAIVDTGSITGGAANAHLALASASERLRKMEAEIGVPLLHRHARGVTMTEAGEVLARHARPLLAQQQRL
RQAMHAFACGQRGTLRLFANTSAMSAFLPGKLAAWMAAHPAVQIETEERTSADIVSCILAGVAQAGVVSDAVDPGPLRLD
PVADDPLVLIVPPARPLAEKEEVAFAAVLNEPLVALYQTSALQQHIEQHAAELGQALNVRVRMSHFAGLCEMVAHGAGGA
ILPRVIAERYRQRYSFAVIGLQDRWAQRRLCLCYQDDASLSPAMRRLLEWLRQP
>Mature_294_residues
MRLDLADLRLFIAIVDTGSITGGAANAHLALASASERLRKMEAEIGVPLLHRHARGVTMTEAGEVLARHARPLLAQQQRL
RQAMHAFACGQRGTLRLFANTSAMSAFLPGKLAAWMAAHPAVQIETEERTSADIVSCILAGVAQAGVVSDAVDPGPLRLD
PVADDPLVLIVPPARPLAEKEEVAFAAVLNEPLVALYQTSALQQHIEQHAAELGQALNVRVRMSHFAGLCEMVAHGAGGA
ILPRVIAERYRQRYSFAVIGLQDRWAQRRLCLCYQDDASLSPAMRRLLEWLRQP

Specific function: Positively regulates the cynTSX operon, and negatively regulates its own transcription. Binds specifically to the cynR- cynTSX intergenic region [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI157672245, Length=243, Percent_Identity=25.9259259259259, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI145693105, Length=288, Percent_Identity=23.6111111111111, Blast_Score=95, Evalue=5e-21,
Organism=Escherichia coli, GI1787806, Length=281, Percent_Identity=23.1316725978648, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1790399, Length=290, Percent_Identity=22.4137931034483, Blast_Score=74, Evalue=9e-15,
Organism=Escherichia coli, GI1790208, Length=282, Percent_Identity=26.5957446808511, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI1788481, Length=290, Percent_Identity=21.7241379310345, Blast_Score=71, Evalue=7e-14,
Organism=Escherichia coli, GI1787879, Length=202, Percent_Identity=25.2475247524752, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1788748, Length=246, Percent_Identity=25.609756097561, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI1789639, Length=148, Percent_Identity=27.027027027027, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1788297, Length=131, Percent_Identity=33.587786259542, Blast_Score=66, Evalue=2e-12,
Organism=Escherichia coli, GI1787128, Length=166, Percent_Identity=25.9036144578313, Blast_Score=65, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 31959; Mature: 31959

Theoretical pI: Translated: 7.96; Mature: 7.96

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLDLADLRLFIAIVDTGSITGGAANAHLALASASERLRKMEAEIGVPLLHRHARGVTMT
CCCCHHHHEEEEEEEECCCCCCCCCCHHEEHHHHHHHHHHHHHHCCCHHHHHHCCCCCHH
EAGEVLARHARPLLAQQQRLRQAMHAFACGQRGTLRLFANTSAMSAFLPGKLAAWMAAHP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCHHHHHHCCHHHHHHHHHCC
AVQIETEERTSADIVSCILAGVAQAGVVSDAVDPGPLRLDPVADDPLVLIVPPARPLAEK
CEEEECCCCHHHHHHHHHHHHHHHHCHHHCCCCCCCEEECCCCCCCEEEEECCCCCCCHH
EEVAFAAVLNEPLVALYQTSALQQHIEQHAAELGQALNVRVRMSHFAGLCEMVAHGAGGA
HHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEHHHHHHHHHHHHHCCCCCH
ILPRVIAERYRQRYSFAVIGLQDRWAQRRLCLCYQDDASLSPAMRRLLEWLRQP
HHHHHHHHHHHHHHCEEEEECHHHHHCCCEEEEECCCCCCCHHHHHHHHHHCCC
>Mature Secondary Structure
MRLDLADLRLFIAIVDTGSITGGAANAHLALASASERLRKMEAEIGVPLLHRHARGVTMT
CCCCHHHHEEEEEEEECCCCCCCCCCHHEEHHHHHHHHHHHHHHCCCHHHHHHCCCCCHH
EAGEVLARHARPLLAQQQRLRQAMHAFACGQRGTLRLFANTSAMSAFLPGKLAAWMAAHP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCHHHHHHCCHHHHHHHHHCC
AVQIETEERTSADIVSCILAGVAQAGVVSDAVDPGPLRLDPVADDPLVLIVPPARPLAEK
CEEEECCCCHHHHHHHHHHHHHHHHCHHHCCCCCCCEEECCCCCCCEEEEECCCCCCCHH
EEVAFAAVLNEPLVALYQTSALQQHIEQHAAELGQALNVRVRMSHFAGLCEMVAHGAGGA
HHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEHHHHHHHHHHHHHCCCCCH
ILPRVIAERYRQRYSFAVIGLQDRWAQRRLCLCYQDDASLSPAMRRLLEWLRQP
HHHHHHHHHHHHHHCEEEEECHHHHHCCCEEEEECCCCCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]