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The map label for this gene is cbiF [H]

Identifier: 150401764

GI number: 150401764

Start: 1402379

End: 1403143

Strand: Reverse

Name: cbiF [H]

Synonym: Maeo_1342

Alternate gene names: 150401764

Gene position: 1403143-1402379 (Counterclockwise)

Preceding gene: 150401765

Following gene: 150401761

Centisome position: 89.4

GC content: 34.64

Gene sequence:

>765_bases
ATGAATAGAAAAATTACAATAGTTGGAGCTGGTTCAGGAGCTCCCGATTTAATAACTGTAAGAGGAGCAAAAGCTATTGA
AAATGCAGACATAATTATTTATGCTGGTTCTTTGGTAAATAAAGAAGTTTTAAACTACAATAAAAAAAATGCCAAAATCT
ACAACAGTGCTACAATGCATCTTGGGGAAGTAATTGGCGTAATAGTAGATGGAGTAAATAATGGTTTAAATGTTGTTAGG
GTGCATACTGGAGACCCCTCAATTTATGGGGCAATTAAAGAACAAATGGACGAATTAAAAAAATATGATATTGGCGTAGA
TATTGTTCCCGGTGTATCTTCCCTATTTGGTGCTATGAGCTCATTACAGGCAGAATTAACACTTCCAGATGTTTCCCAAA
CCATCATAATAACTCGACCAGAAGGTAGAACCCCAAAACCACAATCCGAAAAAATAAGCGATTTGGCAAAACATAAGGCC
ACAATGGCTATTTATTTGGGCGTTGGCATGATTGATAAGGTTGTAAATGAGCTATTGGAAGGTTATGAGTTGGATACTCC
TGTTGCCGTAGTTTATCATGCAACATGGGAAGATGAAAAAATAATCAGAGGAACATTAAAAAACATAGCTAAAAAGGTAA
AAGAGGAAGGAATTACAAAAACAGCACTTATAATTGTAGGTGAGGTTTTAGACCCTAAACGGTATGAATACTCTAAGTTA
TATGATAAAACTTTTGAAACCGAATATAGAAAAGCAGAAAAATAA

Upstream 100 bases:

>100_bases
TATGACGAAGAAGAAATATTAAGGGGATTTTGGAGATGGGACGAACTCCCCCCTAAAATGAGAGAACTTAGAAGAAATAT
AGAAAAAAGGTGTGTGACTA

Downstream 100 bases:

>100_bases
ATAATATATTTTATTTTATATTTTGTCCAATATTATTCTTAGATCTGTTTTCTTTATGATATCTTCTTCATTTTCAATCA
AATTATTTAATTTATTTTTT

Product: precorrin-4 C11-methyltransferase

Products: S-adenosyl-L-homocysteine; Precorrin 5

Alternate protein names: Cobalt-precorrin-3 methylase [H]

Number of amino acids: Translated: 254; Mature: 254

Protein sequence:

>254_residues
MNRKITIVGAGSGAPDLITVRGAKAIENADIIIYAGSLVNKEVLNYNKKNAKIYNSATMHLGEVIGVIVDGVNNGLNVVR
VHTGDPSIYGAIKEQMDELKKYDIGVDIVPGVSSLFGAMSSLQAELTLPDVSQTIIITRPEGRTPKPQSEKISDLAKHKA
TMAIYLGVGMIDKVVNELLEGYELDTPVAVVYHATWEDEKIIRGTLKNIAKKVKEEGITKTALIIVGEVLDPKRYEYSKL
YDKTFETEYRKAEK

Sequences:

>Translated_254_residues
MNRKITIVGAGSGAPDLITVRGAKAIENADIIIYAGSLVNKEVLNYNKKNAKIYNSATMHLGEVIGVIVDGVNNGLNVVR
VHTGDPSIYGAIKEQMDELKKYDIGVDIVPGVSSLFGAMSSLQAELTLPDVSQTIIITRPEGRTPKPQSEKISDLAKHKA
TMAIYLGVGMIDKVVNELLEGYELDTPVAVVYHATWEDEKIIRGTLKNIAKKVKEEGITKTALIIVGEVLDPKRYEYSKL
YDKTFETEYRKAEK
>Mature_254_residues
MNRKITIVGAGSGAPDLITVRGAKAIENADIIIYAGSLVNKEVLNYNKKNAKIYNSATMHLGEVIGVIVDGVNNGLNVVR
VHTGDPSIYGAIKEQMDELKKYDIGVDIVPGVSSLFGAMSSLQAELTLPDVSQTIIITRPEGRTPKPQSEKISDLAKHKA
TMAIYLGVGMIDKVVNELLEGYELDTPVAVVYHATWEDEKIIRGTLKNIAKKVKEEGITKTALIIVGEVLDPKRYEYSKL
YDKTFETEYRKAEK

Specific function: Catalyzes the methylation of C-11 in cobalt-precorrin-4 to form cobalt-precorrin-5 [H]

COG id: COG2875

COG function: function code H; Precorrin-4 methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the precorrin methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1789768, Length=235, Percent_Identity=27.6595744680851, Blast_Score=90, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6322922, Length=235, Percent_Identity=25.531914893617, Blast_Score=82, Evalue=7e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000878
- InterPro:   IPR014777
- InterPro:   IPR014776
- InterPro:   IPR006362
- InterPro:   IPR003043 [H]

Pfam domain/function: PF00590 TP_methylase [H]

EC number: 2.1.1.133

Molecular weight: Translated: 27879; Mature: 27879

Theoretical pI: Translated: 6.98; Mature: 6.98

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRKITIVGAGSGAPDLITVRGAKAIENADIIIYAGSLVNKEVLNYNKKNAKIYNSATMH
CCCEEEEEECCCCCCCEEEECCCCCCCCCCEEEEECCHHHHHHHCCCCCCCEEECHHHHH
LGEVIGVIVDGVNNGLNVVRVHTGDPSIYGAIKEQMDELKKYDIGVDIVPGVSSLFGAMS
HHHHHHHHHHCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHCCCCEECCCHHHHHHHHH
SLQAELTLPDVSQTIIITRPEGRTPKPQSEKISDLAKHKATMAIYLGVGMIDKVVNELLE
HHEEEEECCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHEEEEEECHHHHHHHHHHHHC
GYELDTPVAVVYHATWEDEKIIRGTLKNIAKKVKEEGITKTALIIVGEVLDPKRYEYSKL
CCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCHHHHHH
YDKTFETEYRKAEK
HHHHHHHHHHHCCC
>Mature Secondary Structure
MNRKITIVGAGSGAPDLITVRGAKAIENADIIIYAGSLVNKEVLNYNKKNAKIYNSATMH
CCCEEEEEECCCCCCCEEEECCCCCCCCCCEEEEECCHHHHHHHCCCCCCCEEECHHHHH
LGEVIGVIVDGVNNGLNVVRVHTGDPSIYGAIKEQMDELKKYDIGVDIVPGVSSLFGAMS
HHHHHHHHHHCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHCCCCEECCCHHHHHHHHH
SLQAELTLPDVSQTIIITRPEGRTPKPQSEKISDLAKHKATMAIYLGVGMIDKVVNELLE
HHEEEEECCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHEEEEEECHHHHHHHHHHHHC
GYELDTPVAVVYHATWEDEKIIRGTLKNIAKKVKEEGITKTALIIVGEVLDPKRYEYSKL
CCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCHHHHHH
YDKTFETEYRKAEK
HHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: S-adenosyl-L-methionine; Precorrin 4

Specific reaction: S-adenosyl-L-methionine + precorrin-4 = S-adenosyl-L-homocysteine + precorrin-5

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]