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The map label for this gene is pyrB

Identifier: 150401761

GI number: 150401761

Start: 1400556

End: 1401500

Strand: Reverse

Name: pyrB

Synonym: Maeo_1339

Alternate gene names: 150401761

Gene position: 1401500-1400556 (Counterclockwise)

Preceding gene: 150401764

Following gene: 150401760

Centisome position: 89.3

GC content: 32.49

Gene sequence:

>945_bases
ATGGAGCACTTTATATCTATAAGAGACATCGGGAAAAAAGAAATATTAAATATATTGGAAGAAGCTGAAAAAATGGAGGA
GCTCCTAAATTCAGAAAAACATTCTAATATAATGAATGGAAAAATACTTGCCACATTATTTTACGAACCATCAACTAGAA
CTAGATTATCCTTTGAAACTGCCATGAAAAGATTGGGGGGAACCGTAGTGGGATTTACAGACATAGCAAATACCTCAGTT
ATGAAAGGGGAGACCTTGCCCGATACAATAAAGGTAATTAACGGCTATGCCGATTTAATTGTTATGAGACATCCCTCCGA
TGGAGCTCCAAGGTTGGCATGTGAATACTCAAATATACCCATAATTAATGCAGGAGATGGAAGCAATCAACACCCCTCCC
AAACAATGTTGGATCTATACACAATTAAACGAGAAATTGGACATATAGATAATATAAAAATAGCTTTTATCGGGGATTTA
AAGTATGGGCGGACTGTTCATTCATTATGTCATGCTTTATCATTTTTTGAGAACATTGAAATTGTATTTATAGCCCCAAA
AGAGCTCAAAATTCCAAAAGAAATAACCGATGATTTAGACAATAAAAATAAACTATACAACAACAATATAAAATATTCGG
AAACAGGCGATATTGATTTAACTGGTATGGATGTAGTTTATATGACAAGAATACAGAAAGAACGGTTTCCAGATTTAAGC
GAATACCAGAAAGTAAAAGGAACATACAAATTAACCAGAGAACATGTGGAAGATAAAGATTTAATTATAATGCACCCACT
TCCACGAGTAGATGAAATTGATATTTCTGTTGATGAGCTCCCTCAAGCAAAATATTTTAAACAGTCATTTTATGGTGTGC
CGGTAAGAATGGCTATTTTAAAAATATTAAATGAAAAAAATAAAAATAAAAAATCTGAAATATAA

Upstream 100 bases:

>100_bases
TATATAATCTTTTGACAAACAGGAACAATTTTTTGAACATACTAAATATCGTTAATATAATGACAGTTAAAATATATAAA
TCATTATTATTGGTGATTAA

Downstream 100 bases:

>100_bases
TATAATGCCATAGTTAAGTATATATATTATTATTAATATTTTATAACTACTCGCATGACTCTCTCAAAATTGGACTTATA
TTAATAATTATTGAAAAACG

Product: aspartate carbamoyltransferase catalytic subunit

Products: NA

Alternate protein names: Aspartate transcarbamylase; ATCase

Number of amino acids: Translated: 314; Mature: 314

Protein sequence:

>314_residues
MEHFISIRDIGKKEILNILEEAEKMEELLNSEKHSNIMNGKILATLFYEPSTRTRLSFETAMKRLGGTVVGFTDIANTSV
MKGETLPDTIKVINGYADLIVMRHPSDGAPRLACEYSNIPIINAGDGSNQHPSQTMLDLYTIKREIGHIDNIKIAFIGDL
KYGRTVHSLCHALSFFENIEIVFIAPKELKIPKEITDDLDNKNKLYNNNIKYSETGDIDLTGMDVVYMTRIQKERFPDLS
EYQKVKGTYKLTREHVEDKDLIIMHPLPRVDEIDISVDELPQAKYFKQSFYGVPVRMAILKILNEKNKNKKSEI

Sequences:

>Translated_314_residues
MEHFISIRDIGKKEILNILEEAEKMEELLNSEKHSNIMNGKILATLFYEPSTRTRLSFETAMKRLGGTVVGFTDIANTSV
MKGETLPDTIKVINGYADLIVMRHPSDGAPRLACEYSNIPIINAGDGSNQHPSQTMLDLYTIKREIGHIDNIKIAFIGDL
KYGRTVHSLCHALSFFENIEIVFIAPKELKIPKEITDDLDNKNKLYNNNIKYSETGDIDLTGMDVVYMTRIQKERFPDLS
EYQKVKGTYKLTREHVEDKDLIIMHPLPRVDEIDISVDELPQAKYFKQSFYGVPVRMAILKILNEKNKNKKSEI
>Mature_314_residues
MEHFISIRDIGKKEILNILEEAEKMEELLNSEKHSNIMNGKILATLFYEPSTRTRLSFETAMKRLGGTVVGFTDIANTSV
MKGETLPDTIKVINGYADLIVMRHPSDGAPRLACEYSNIPIINAGDGSNQHPSQTMLDLYTIKREIGHIDNIKIAFIGDL
KYGRTVHSLCHALSFFENIEIVFIAPKELKIPKEITDDLDNKNKLYNNNIKYSETGDIDLTGMDVVYMTRIQKERFPDLS
EYQKVKGTYKLTREHVEDKDLIIMHPLPRVDEIDISVDELPQAKYFKQSFYGVPVRMAILKILNEKNKNKKSEI

Specific function: Pyrimidine biosynthesis; second step. [C]

COG id: COG0540

COG function: function code F; Aspartate carbamoyltransferase, catalytic chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATCase/OTCase family

Homologues:

Organism=Homo sapiens, GI18105007, Length=304, Percent_Identity=39.8026315789474, Blast_Score=247, Evalue=1e-65,
Organism=Homo sapiens, GI38788445, Length=262, Percent_Identity=27.0992366412214, Blast_Score=71, Evalue=2e-12,
Organism=Escherichia coli, GI2367364, Length=308, Percent_Identity=48.7012987012987, Blast_Score=287, Evalue=8e-79,
Organism=Escherichia coli, GI1790703, Length=278, Percent_Identity=23.3812949640288, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI1786469, Length=228, Percent_Identity=21.9298245614035, Blast_Score=63, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI193204318, Length=317, Percent_Identity=42.2712933753943, Blast_Score=236, Evalue=2e-62,
Organism=Saccharomyces cerevisiae, GI6322331, Length=304, Percent_Identity=41.1184210526316, Blast_Score=233, Evalue=3e-62,
Organism=Saccharomyces cerevisiae, GI6322373, Length=298, Percent_Identity=25.503355704698, Blast_Score=70, Evalue=4e-13,
Organism=Drosophila melanogaster, GI24642586, Length=308, Percent_Identity=40.9090909090909, Blast_Score=243, Evalue=2e-64,

Paralogues:

None

Copy number: 2080 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 14328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): PYRB_META3 (A6UWP3)

Other databases:

- EMBL:   CP000743
- RefSeq:   YP_001325527.1
- ProteinModelPortal:   A6UWP3
- SMR:   A6UWP3
- STRING:   A6UWP3
- GeneID:   5327296
- GenomeReviews:   CP000743_GR
- KEGG:   mae:Maeo_1339
- eggNOG:   arNOG04260
- HOGENOM:   HBG579429
- OMA:   FKQSFYG
- ProtClustDB:   PRK00856
- BioCyc:   MAEO419665:MAEO_1339-MONOMER
- HAMAP:   MF_00001
- InterPro:   IPR006132
- InterPro:   IPR006130
- InterPro:   IPR006131
- InterPro:   IPR002082
- PRINTS:   PR00100
- PRINTS:   PR00101
- TIGRFAMs:   TIGR00670

Pfam domain/function: PF00185 OTCace; PF02729 OTCace_N; SSF53671 Asp/Orn_carbamoyltranf

EC number: =2.1.3.2

Molecular weight: Translated: 35897; Mature: 35897

Theoretical pI: Translated: 6.43; Mature: 6.43

Prosite motif: PS00097 CARBAMOYLTRANSFERASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEHFISIRDIGKKEILNILEEAEKMEELLNSEKHSNIMNGKILATLFYEPSTRTRLSFET
CCCEEEHHHCCHHHHHHHHHHHHHHHHHHCCHHHCCCCCCEEEEEEEECCCCCCEEEHHH
AMKRLGGTVVGFTDIANTSVMKGETLPDTIKVINGYADLIVMRHPSDGAPRLACEYSNIP
HHHHHCCEEEEEHHHCCCCEECCCCCCHHHHHHCCCEEEEEEECCCCCCCEEEEECCCCC
IINAGDGSNQHPSQTMLDLYTIKREIGHIDNIKIAFIGDLKYGRTVHSLCHALSFFENIE
EEECCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCHHHHHHHHHHHHHCCCE
IVFIAPKELKIPKEITDDLDNKNKLYNNNIKYSETGDIDLTGMDVVYMTRIQKERFPDLS
EEEECCCCCCCCHHHHHCCCCCCCEECCCCCCCCCCCEEECCCCHHHHHHHHHHHCCCHH
EYQKVKGTYKLTREHVEDKDLIIMHPLPRVDEIDISVDELPQAKYFKQSFYGVPVRMAIL
HHHHHCCHHHHHHHHCCCCCEEEECCCCCCCCCCCCHHHCCCHHHHHHHHCCCHHHHHHH
KILNEKNKNKKSEI
HHHHHCCCCCCCCC
>Mature Secondary Structure
MEHFISIRDIGKKEILNILEEAEKMEELLNSEKHSNIMNGKILATLFYEPSTRTRLSFET
CCCEEEHHHCCHHHHHHHHHHHHHHHHHHCCHHHCCCCCCEEEEEEEECCCCCCEEEHHH
AMKRLGGTVVGFTDIANTSVMKGETLPDTIKVINGYADLIVMRHPSDGAPRLACEYSNIP
HHHHHCCEEEEEHHHCCCCEECCCCCCHHHHHHCCCEEEEEEECCCCCCCEEEEECCCCC
IINAGDGSNQHPSQTMLDLYTIKREIGHIDNIKIAFIGDLKYGRTVHSLCHALSFFENIE
EEECCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCHHHHHHHHHHHHHCCCE
IVFIAPKELKIPKEITDDLDNKNKLYNNNIKYSETGDIDLTGMDVVYMTRIQKERFPDLS
EEEECCCCCCCCHHHHHCCCCCCCEECCCCCCCCCCCEEECCCCHHHHHHHHHHHCCCHH
EYQKVKGTYKLTREHVEDKDLIIMHPLPRVDEIDISVDELPQAKYFKQSFYGVPVRMAIL
HHHHHCCHHHHHHHHCCCCCEEEECCCCCCCCCCCCHHHCCCHHHHHHHHCCCHHHHHHH
KILNEKNKNKKSEI
HHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA