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The map label for this gene is cysH [C]

Identifier: 150401765

GI number: 150401765

Start: 1403136

End: 1404461

Strand: Reverse

Name: cysH [C]

Synonym: Maeo_1343

Alternate gene names: 150401765

Gene position: 1404461-1403136 (Counterclockwise)

Preceding gene: 150401766

Following gene: 150401764

Centisome position: 89.48

GC content: 28.96

Gene sequence:

>1326_bases
ATGTATAGTGATTACAATAATAATGATAACAATATGGATAAAACAATGTTCTCAAAATTTAAAGAGGATACAAAATTTGC
ATCAAAATATGAAATTGATATATTAAATAAATTAACAAATAAAAACTTCAATTATGATGATTTAATATTATTGGAGAAAT
TGGCAGGAGATGATTATAGAAAAAGAGTTTATTTGGACGAAAATGTTCAAATAGGAACTATGGAGTTCGATTTATTAGAT
TTAGAATGGAAATTTACACCTTCGCCATATTATTATTATCTTGAAAGTCCAAAATTAAAATTGGTGCCAACAAAAAGAAG
ATTAAAAGGAAAATATTTAAAAGAAGAATATATTGAAAATATTGATGAATATAAAACCATTGTAGAAAATACGGACTATT
TTGTTGGTATTGATATGGGGAAATTTTTGGGAGTGGGAATAAAAAGAGATAACAGAATTAAAGTAAAAGACCTCCAAAAG
AAAAATGATGAAATAAGAATTAAAAAAATAGAAAACTACCTACAAGATAACAAAGAAAGAATAAATAAACTTGTTCAACA
TTCAAAAGATATTCTTGTAGAATATATTCAAAAATACGAAAATAAAAATTATGTAATAAATGTATCTTTTAGTGGTGGAA
AGGATAGTGCCGTATCAACTTTATTAGCTACCGAAATTATGGAGGATATGGATGTATTATTTATAGATACAGGGCTTGAA
TATCCAGAAACCAACCAATATGTAAAAGATTTCGCAAAAAAATATGATTTAAATTTACATACAATAGATGGAGATAATTT
CTGGGATGAAGTGGATGAAGAAGGCATACCAACAAAAGACGATAGATGGTGCAATAGCACATGTAAATTGATACCATTGG
AAGAATTCTTTGAGGAACATTACCCAAACAAAAAAATATTGACAATAGACGGCTCCCGAAAATTAGAAAGTTTTGCACGG
GCAAATTTAGAATATACAAGACAGAGCGGATTTATTGATGCCCAAACCAATTTATTCCCAATTCTTGATTGGAATTCTAT
TGACATTTGGAGCTACATATTTTTAAATGATGTATTGTATAATCCAATGTATGAAGAAGGATTTGAAAGAATTGGTTGTT
ATATGTGTCCAGCGGCACTTAATAGTGAATTTTTAAGAGTAAAGGAGCTCCACCCAAAATTATGGGATAAGTGGGCATGC
CAGTTAAGAAAAGAAGACTATGACGAAGAAGAAATATTAAGGGGATTTTGGAGATGGGACGAACTCCCCCCTAAAATGAG
AGAACTTAGAAGAAATATAGAAAAAAGGTGTGTGACTAATGAATAG

Upstream 100 bases:

>100_bases
ATGCAAATCTTTTTACCCAAATAAAAAACAAAAACCTTTCGGTTTTATGACCTACATCTTGATAGGGCGATAAAATTAGA
GTTAATTAAAGTGATAAAAA

Downstream 100 bases:

>100_bases
AAAAATTACAATAGTTGGAGCTGGTTCAGGAGCTCCCGATTTAATAACTGTAAGAGGAGCAAAAGCTATTGAAAATGCAG
ACATAATTATTTATGCTGGT

Product: hypothetical protein

Products: H+; Adenosine 3',5'-bisphosphate; Sulfite; Oxidized thioredoxin [C]

Alternate protein names: NA

Number of amino acids: Translated: 441; Mature: 441

Protein sequence:

>441_residues
MYSDYNNNDNNMDKTMFSKFKEDTKFASKYEIDILNKLTNKNFNYDDLILLEKLAGDDYRKRVYLDENVQIGTMEFDLLD
LEWKFTPSPYYYYLESPKLKLVPTKRRLKGKYLKEEYIENIDEYKTIVENTDYFVGIDMGKFLGVGIKRDNRIKVKDLQK
KNDEIRIKKIENYLQDNKERINKLVQHSKDILVEYIQKYENKNYVINVSFSGGKDSAVSTLLATEIMEDMDVLFIDTGLE
YPETNQYVKDFAKKYDLNLHTIDGDNFWDEVDEEGIPTKDDRWCNSTCKLIPLEEFFEEHYPNKKILTIDGSRKLESFAR
ANLEYTRQSGFIDAQTNLFPILDWNSIDIWSYIFLNDVLYNPMYEEGFERIGCYMCPAALNSEFLRVKELHPKLWDKWAC
QLRKEDYDEEEILRGFWRWDELPPKMRELRRNIEKRCVTNE

Sequences:

>Translated_441_residues
MYSDYNNNDNNMDKTMFSKFKEDTKFASKYEIDILNKLTNKNFNYDDLILLEKLAGDDYRKRVYLDENVQIGTMEFDLLD
LEWKFTPSPYYYYLESPKLKLVPTKRRLKGKYLKEEYIENIDEYKTIVENTDYFVGIDMGKFLGVGIKRDNRIKVKDLQK
KNDEIRIKKIENYLQDNKERINKLVQHSKDILVEYIQKYENKNYVINVSFSGGKDSAVSTLLATEIMEDMDVLFIDTGLE
YPETNQYVKDFAKKYDLNLHTIDGDNFWDEVDEEGIPTKDDRWCNSTCKLIPLEEFFEEHYPNKKILTIDGSRKLESFAR
ANLEYTRQSGFIDAQTNLFPILDWNSIDIWSYIFLNDVLYNPMYEEGFERIGCYMCPAALNSEFLRVKELHPKLWDKWAC
QLRKEDYDEEEILRGFWRWDELPPKMRELRRNIEKRCVTNE
>Mature_441_residues
MYSDYNNNDNNMDKTMFSKFKEDTKFASKYEIDILNKLTNKNFNYDDLILLEKLAGDDYRKRVYLDENVQIGTMEFDLLD
LEWKFTPSPYYYYLESPKLKLVPTKRRLKGKYLKEEYIENIDEYKTIVENTDYFVGIDMGKFLGVGIKRDNRIKVKDLQK
KNDEIRIKKIENYLQDNKERINKLVQHSKDILVEYIQKYENKNYVINVSFSGGKDSAVSTLLATEIMEDMDVLFIDTGLE
YPETNQYVKDFAKKYDLNLHTIDGDNFWDEVDEEGIPTKDDRWCNSTCKLIPLEEFFEEHYPNKKILTIDGSRKLESFAR
ANLEYTRQSGFIDAQTNLFPILDWNSIDIWSYIFLNDVLYNPMYEEGFERIGCYMCPAALNSEFLRVKELHPKLWDKWAC
QLRKEDYDEEEILRGFWRWDELPPKMRELRRNIEKRCVTNE

Specific function: Reduction Of Activated Sulfate Into Sulfite. [C]

COG id: COG0175

COG function: function code EH; 3'-phosphoadenosine 5'-phosphosulfate sulfotransferase (PAPS reductase)/FAD synthetase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the PAPS reductase family [H]

Homologues:

Organism=Escherichia coli, GI1789121, Length=177, Percent_Identity=28.2485875706215, Blast_Score=63, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002500
- InterPro:   IPR014729 [H]

Pfam domain/function: PF01507 PAPS_reduct [H]

EC number: 1.8.4.8 [C]

Molecular weight: Translated: 52854; Mature: 52854

Theoretical pI: Translated: 4.71; Mature: 4.71

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYSDYNNNDNNMDKTMFSKFKEDTKFASKYEIDILNKLTNKNFNYDDLILLEKLAGDDYR
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCHH
KRVYLDENVQIGTMEFDLLDLEWKFTPSPYYYYLESPKLKLVPTKRRLKGKYLKEEYIEN
EEEEECCCEEEEEEEEEEEEEEEEECCCCEEEEEECCCEEEECCHHHHHHHHHHHHHHHH
IDEYKTIVENTDYFVGIDMGKFLGVGIKRDNRIKVKDLQKKNDEIRIKKIENYLQDNKER
HHHHHHHHCCCCEEEEECHHHHHCCCCCCCCEEEHHHHHCCCCCEEHHHHHHHHHCCHHH
INKLVQHSKDILVEYIQKYENKNYVINVSFSGGKDSAVSTLLATEIMEDMDVLFIDTGLE
HHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHHHCCCEEEEECCCC
YPETNQYVKDFAKKYDLNLHTIDGDNFWDEVDEEGIPTKDDRWCNSTCKLIPLEEFFEEH
CCCCHHHHHHHHHHHCCEEEEECCCCHHHHHCCCCCCCCCCCCCCCCCCEEEHHHHHHHH
YPNKKILTIDGSRKLESFARANLEYTRQSGFIDAQTNLFPILDWNSIDIWSYIFLNDVLY
CCCCEEEEECCCHHHHHHHHCCCHHHHCCCCEECCCCEEEEEECCCCHHHHHHHHHHHHC
NPMYEEGFERIGCYMCPAALNSEFLRVKELHPKLWDKWACQLRKEDYDEEEILRGFWRWD
CCHHHHHHHHHCCEECCHHHCHHHHHHHHHCHHHHHHHHHHHHHCCCCHHHHHHHHHHCC
ELPPKMRELRRNIEKRCVTNE
CCCHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MYSDYNNNDNNMDKTMFSKFKEDTKFASKYEIDILNKLTNKNFNYDDLILLEKLAGDDYR
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCHH
KRVYLDENVQIGTMEFDLLDLEWKFTPSPYYYYLESPKLKLVPTKRRLKGKYLKEEYIEN
EEEEECCCEEEEEEEEEEEEEEEEECCCCEEEEEECCCEEEECCHHHHHHHHHHHHHHHH
IDEYKTIVENTDYFVGIDMGKFLGVGIKRDNRIKVKDLQKKNDEIRIKKIENYLQDNKER
HHHHHHHHCCCCEEEEECHHHHHCCCCCCCCEEEHHHHHCCCCCEEHHHHHHHHHCCHHH
INKLVQHSKDILVEYIQKYENKNYVINVSFSGGKDSAVSTLLATEIMEDMDVLFIDTGLE
HHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHHHCCCEEEEECCCC
YPETNQYVKDFAKKYDLNLHTIDGDNFWDEVDEEGIPTKDDRWCNSTCKLIPLEEFFEEH
CCCCHHHHHHHHHHHCCEEEEECCCCHHHHHCCCCCCCCCCCCCCCCCCEEEHHHHHHHH
YPNKKILTIDGSRKLESFARANLEYTRQSGFIDAQTNLFPILDWNSIDIWSYIFLNDVLY
CCCCEEEEECCCHHHHHHHHCCCHHHHCCCCEECCCCEEEEEECCCCHHHHHHHHHHHHC
NPMYEEGFERIGCYMCPAALNSEFLRVKELHPKLWDKWACQLRKEDYDEEEILRGFWRWD
CCHHHHHHHHHCCEECCHHHCHHHHHHHHHCHHHHHHHHHHHHHCCCCHHHHHHHHHHCC
ELPPKMRELRRNIEKRCVTNE
CCCHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): 5700 [C]

Specific activity: 99

Km value (mM): 0.01 {5-phosphoadenosine} [C]

Substrates: 3'-Phosphoadenylyl sulfate; Reduced thioredoxin [C]

Specific reaction: 3'-Phosphoadenylyl sulfate + Reduced thioredoxin --> (2) H+ + Adenosine 3',5'-bisphosphate + Sulfite + Oxidized thioredoxin [C]

General reaction: Oxidation; Reduction [C]

Inhibitor: Adenosine3', 5'-bisphosphate; N-Bromosuccinimide; p-Chloromercuribenzoate [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]