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The map label for this gene is thrA [C]

Identifier: 150401106

GI number: 150401106

Start: 693129

End: 694535

Strand: Direct

Name: thrA [C]

Synonym: Maeo_0676

Alternate gene names: 150401106

Gene position: 693129-694535 (Clockwise)

Preceding gene: 150401104

Following gene: 150401112

Centisome position: 44.16

GC content: 34.61

Gene sequence:

>1407_bases
ATGATTACAGTTATGAAATTCGGCGGAACCTCAGTAGGAGACGGACAGAGAATAAGAAATGTTGCAAAAATAATAGTGGA
TAAATCAAATAAAGAAAATACTGATATTGTCGTAGTTACCTCGGCAATGTCTCAAATAACAAACGCACTTGTAAATATGT
CAAAACAGGCCCTTAATGTTAGGGATATTGCTAAAATAAACAATTTTGTTGAAGAATTAAAGGAGAAACATGCAATTGCA
ATAGACGAAGCAGTGAAAAACGACGACATAAGAACAGAAGTAAGGCATGTAATAAACAGCTCCATTGATGAGTTGGGAAA
AGTATTGGTTGGTGTTTCATATTTGGGTGAATTAACCCCGAAATCAAAAGATTATATTCTTTCATTTGGAGAAAGATTAT
CTGCACCAATATTAAGCGGAGCAGTACGAGATTTGGGAAAAAATTCATTATTTTTATTAGGTGGTGAAGCTGGACTTATT
ACTGATGAAAATTATGGCTGTGCAAGACCCATAAAAATAAAAATTAAAGAAAAAGTAAATCCACTATTGGAAGAAAATAT
AATCCCAATAATTACAGGATTTGTAGCAGGGACAGAAAACGGAGACATTACTACATTTGGAAGGGGCGGAAGCGATTATT
CGGCTGCTCTTGTTGGTGCTGGTTTAGATGCCGATACCGTAGAGATATGGACTGATGTGAGCGGCATATTAACATCAGAC
CCAAGAATTGTTAAAAATGTTAAAAGAATACCAAAAATGTCATATATTGAAGCTATGGAGTTAGCATATTTTGGTGCAAA
GGTATTACATCCCCGAACAGTTGAGCCATTAATGGAAAAAAACATACCCCTTAGGATTAAAAATACATTTGAGCCAGAAA
ATGAGGGAACATTTATTACAAATTGCAAAGAATTAAGCAATAGTGTAATGAAAGCCGTATCTGCAATAAGAGATGTATTT
TTAATAAATATCTTTGGAGCTGGTATGGTAGGAGTTAGTGGAACAGCTGCAAGAATATTTTCAGCACTTGGAAGGGCCGA
TGCAAATGTATTATTAATTACACAGGGTTCTTCTGAAACTAATGTTTCTGTGGTAATATATGGTGATGAAGTTGATGCCA
ACAATTGTATGAAGGAGCTCCGGAAAGAATTTAAAAATAGCAATCTTGTAAAGGACATTAGTATAGATGAAAATGTTGCT
GTAATTTCTGCGGTTGGTGTAGGTATGAAAGGCAGCAAAGGTATTGCAGGAAAATTATTTGGAGCTGTGGCAGAAAGTGG
GGCAAATATAAAAATGATAGCCCAGGGTTCTTCCGAAGTAAATATATCATTTGTAATTGGAGAAGAAGAGCTTGAAAATT
GTTTAAGAATATTGCATGGTAGATTTATAGAAAATGCAGAAAATTAA

Upstream 100 bases:

>100_bases
TATTTTCCTTATTTATTATTTTTTTATATGGTATGTTAATTCATTGCATATACTAAATTTATTAGATTATATCTAATTAA
TACTAATTCGGTGTATTATT

Downstream 100 bases:

>100_bases
AAAACGAGGGAGCTCCCACAACGAAAAACCATGGAGCTCCCAAATACATATTAAAAATTTTATTTTTAATTCTTTTTTAA
AATATTAAATATAAAAATAG

Product: aspartate kinase

Products: NA

Alternate protein names: Aspartate kinase [H]

Number of amino acids: Translated: 468; Mature: 468

Protein sequence:

>468_residues
MITVMKFGGTSVGDGQRIRNVAKIIVDKSNKENTDIVVVTSAMSQITNALVNMSKQALNVRDIAKINNFVEELKEKHAIA
IDEAVKNDDIRTEVRHVINSSIDELGKVLVGVSYLGELTPKSKDYILSFGERLSAPILSGAVRDLGKNSLFLLGGEAGLI
TDENYGCARPIKIKIKEKVNPLLEENIIPIITGFVAGTENGDITTFGRGGSDYSAALVGAGLDADTVEIWTDVSGILTSD
PRIVKNVKRIPKMSYIEAMELAYFGAKVLHPRTVEPLMEKNIPLRIKNTFEPENEGTFITNCKELSNSVMKAVSAIRDVF
LINIFGAGMVGVSGTAARIFSALGRADANVLLITQGSSETNVSVVIYGDEVDANNCMKELRKEFKNSNLVKDISIDENVA
VISAVGVGMKGSKGIAGKLFGAVAESGANIKMIAQGSSEVNISFVIGEEELENCLRILHGRFIENAEN

Sequences:

>Translated_468_residues
MITVMKFGGTSVGDGQRIRNVAKIIVDKSNKENTDIVVVTSAMSQITNALVNMSKQALNVRDIAKINNFVEELKEKHAIA
IDEAVKNDDIRTEVRHVINSSIDELGKVLVGVSYLGELTPKSKDYILSFGERLSAPILSGAVRDLGKNSLFLLGGEAGLI
TDENYGCARPIKIKIKEKVNPLLEENIIPIITGFVAGTENGDITTFGRGGSDYSAALVGAGLDADTVEIWTDVSGILTSD
PRIVKNVKRIPKMSYIEAMELAYFGAKVLHPRTVEPLMEKNIPLRIKNTFEPENEGTFITNCKELSNSVMKAVSAIRDVF
LINIFGAGMVGVSGTAARIFSALGRADANVLLITQGSSETNVSVVIYGDEVDANNCMKELRKEFKNSNLVKDISIDENVA
VISAVGVGMKGSKGIAGKLFGAVAESGANIKMIAQGSSEVNISFVIGEEELENCLRILHGRFIENAEN
>Mature_468_residues
MITVMKFGGTSVGDGQRIRNVAKIIVDKSNKENTDIVVVTSAMSQITNALVNMSKQALNVRDIAKINNFVEELKEKHAIA
IDEAVKNDDIRTEVRHVINSSIDELGKVLVGVSYLGELTPKSKDYILSFGERLSAPILSGAVRDLGKNSLFLLGGEAGLI
TDENYGCARPIKIKIKEKVNPLLEENIIPIITGFVAGTENGDITTFGRGGSDYSAALVGAGLDADTVEIWTDVSGILTSD
PRIVKNVKRIPKMSYIEAMELAYFGAKVLHPRTVEPLMEKNIPLRIKNTFEPENEGTFITNCKELSNSVMKAVSAIRDVF
LINIFGAGMVGVSGTAARIFSALGRADANVLLITQGSSETNVSVVIYGDEVDANNCMKELRKEFKNSNLVKDISIDENVA
VISAVGVGMKGSKGIAGKLFGAVAESGANIKMIAQGSSEVNISFVIGEEELENCLRILHGRFIENAEN

Specific function: Catalyzes 2 nonconsecutive reactions in the common biosynthetic pathway leading from Asp to diaminopimelate and Lys, to Met, and to Thr and Ile. [C]

COG id: COG0527

COG function: function code E; Aspartokinases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 ACT domains [H]

Homologues:

Organism=Escherichia coli, GI1786183, Length=464, Percent_Identity=35.5603448275862, Blast_Score=266, Evalue=2e-72,
Organism=Escherichia coli, GI1790455, Length=476, Percent_Identity=28.781512605042, Blast_Score=168, Evalue=5e-43,
Organism=Escherichia coli, GI1790376, Length=466, Percent_Identity=24.2489270386266, Blast_Score=107, Evalue=1e-24,
Organism=Saccharomyces cerevisiae, GI6320893, Length=501, Percent_Identity=29.5409181636727, Blast_Score=205, Evalue=1e-53,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002912
- InterPro:   IPR001048
- InterPro:   IPR005260
- InterPro:   IPR001341
- InterPro:   IPR018042 [H]

Pfam domain/function: PF00696 AA_kinase; PF01842 ACT [H]

EC number: =2.7.2.4 [H]

Molecular weight: Translated: 50413; Mature: 50413

Theoretical pI: Translated: 5.16; Mature: 5.16

Prosite motif: PS00324 ASPARTOKINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MITVMKFGGTSVGDGQRIRNVAKIIVDKSNKENTDIVVVTSAMSQITNALVNMSKQALNV
CEEEEEECCCCCCCCHHHHHHHHHHHCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHCCH
RDIAKINNFVEELKEKHAIAIDEAVKNDDIRTEVRHVINSSIDELGKVLVGVSYLGELTP
HHHHHHHHHHHHHHHHCCEEHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
KSKDYILSFGERLSAPILSGAVRDLGKNSLFLLGGEAGLITDENYGCARPIKIKIKEKVN
CCCHHHHHHHHHHCCHHHHHHHHHCCCCCEEEEECCCCEEECCCCCCCCCEEEEEHHHCC
PLLEENIIPIITGFVAGTENGDITTFGRGGSDYSAALVGAGLDADTVEIWTDVSGILTSD
HHHHCCCHHEEEEEEECCCCCCEEEECCCCCCCCCEEEECCCCCCHHEEECCCCHHCCCC
PRIVKNVKRIPKMSYIEAMELAYFGAKVLHPRTVEPLMEKNIPLRIKNTFEPENEGTFIT
CHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCEEEECCCCCCCCCCEEE
NCKELSNSVMKAVSAIRDVFLINIFGAGMVGVSGTAARIFSALGRADANVLLITQGSSET
CHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCCEEEEECCCCCC
NVSVVIYGDEVDANNCMKELRKEFKNSNLVKDISIDENVAVISAVGVGMKGSKGIAGKLF
EEEEEEECCCCCHHHHHHHHHHHHCCCCCEEECCCCCCEEEEEEECCCCCCCCCCHHHHH
GAVAESGANIKMIAQGSSEVNISFVIGEEELENCLRILHGRFIENAEN
HHHHCCCCCEEEEECCCCCEEEEEEECHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MITVMKFGGTSVGDGQRIRNVAKIIVDKSNKENTDIVVVTSAMSQITNALVNMSKQALNV
CEEEEEECCCCCCCCHHHHHHHHHHHCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHCCH
RDIAKINNFVEELKEKHAIAIDEAVKNDDIRTEVRHVINSSIDELGKVLVGVSYLGELTP
HHHHHHHHHHHHHHHHCCEEHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
KSKDYILSFGERLSAPILSGAVRDLGKNSLFLLGGEAGLITDENYGCARPIKIKIKEKVN
CCCHHHHHHHHHHCCHHHHHHHHHCCCCCEEEEECCCCEEECCCCCCCCCEEEEEHHHCC
PLLEENIIPIITGFVAGTENGDITTFGRGGSDYSAALVGAGLDADTVEIWTDVSGILTSD
HHHHCCCHHEEEEEEECCCCCCEEEECCCCCCCCCEEEECCCCCCHHEEECCCCHHCCCC
PRIVKNVKRIPKMSYIEAMELAYFGAKVLHPRTVEPLMEKNIPLRIKNTFEPENEGTFIT
CHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCEEEECCCCCCCCCCEEE
NCKELSNSVMKAVSAIRDVFLINIFGAGMVGVSGTAARIFSALGRADANVLLITQGSSET
CHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCCEEEEECCCCCC
NVSVVIYGDEVDANNCMKELRKEFKNSNLVKDISIDENVAVISAVGVGMKGSKGIAGKLF
EEEEEEECCCCCHHHHHHHHHHHHCCCCCEEECCCCCCEEEEEEECCCCCCCCCCHHHHH
GAVAESGANIKMIAQGSSEVNISFVIGEEELENCLRILHGRFIENAEN
HHHHCCCCCEEEEECCCCCEEEEEEECHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]