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The map label for this gene is ilvB [H]

Identifier: 150401112

GI number: 150401112

Start: 700543

End: 702342

Strand: Direct

Name: ilvB [H]

Synonym: Maeo_0682

Alternate gene names: 150401112

Gene position: 700543-702342 (Clockwise)

Preceding gene: 150401106

Following gene: 150401113

Centisome position: 44.63

GC content: 35.22

Gene sequence:

>1800_bases
ATGAACGGAGCAGAGGCTATGATAAAAGCACTTGAAGCTGAAAAAGTCGAAATATTATTTGGTTATCCCGGGGGAGCTCT
ACTACCATTTTACGATGCACTACATCATAGTGATTTAATACATCTTTTAACACGACATGAACAAGCCGCCGCCCATGCCG
CAGACGGATATGCCAGAGCAAGTGGCAAAGTTGGAGTATGTATAGGAACATCTGGACCAGGTGCAACTAATCTTGTTACA
GGAGTGGCTACAGCACATTCTGATTCCTCTCCAATGGTGGCATTGACAGGGCAAGTTCCAACAAAATTAATAGGAAATGA
TGCATTTCAGGAAATAGATGCCCTTGGATTATTTATGCCCATTGTGAAGCATAACTTTCAAATTCAAAAAACTTGTCAAA
TACCAGAGATTTTCAGAAGTGCTTTTGAAATAGCCCAGACTGGAAGACCTGGACCCGTGCATATCGATTTACCAAAAGAT
GTTCAAGAATTAGAATTAGATATTGATAAACACCCAATACCGTCAAAAGTAAAATTAATAGGATATAATCCAACCACCAT
TGGACATCCGCGACAAATAAAAAAAGCAATTAAATTAATCGCTTCTGCTAAAAGACCGATAATTTTAGCAGGTGGTGGAG
TACTATTAAGTGGAGCTAATGAAGAATTATTAAAATTAGTGGAATTATTAAACATACCTGTATGTACAACTTTAATGGGT
AAAGGTTGTATATCTGAAAATCATCCCCTTGCCCTTGGTATGGTTGGTATGCATGGGACAAAACCAGCTAATTATTGTCT
ATCTGAAAGTGATGTTTTAATATCCATTGGTTGTAGGTTCTCCGATAGAATTACAGGAGATATAAAAAGTTTTGCTACAA
ATGCAAAAATTATCCATATTGATATAGACCCCGCCGAAATTGGCAAAAATGTAGCCGTAGATGTGCCAATTGTGGGAGAT
GCTAAATTAATATTAAAAGAATTAATAAAACAGCTGGATTATATCATCAATAAAGATAGTAAGGAAAATAACGATAAAGA
AAATATATCACAATGGATAGAAAATGTTAATTCATTGAAAAAATCCTCAATTCCTGTAATGGATTATGATGATATTCCAA
TAAAACCACAAAAAATAGTAAAAGAACTTATGGCCGTTATTGACGATTTAAATATTAATAAAAATACCATAATTACAACC
GATGTTGGACAAAATCAAATGTGGATGGCCCACTACTTTAAAACCCAAACTCCACGGTCATTCTTATCCTCGGGAGGATT
AGGAACTATGGGATTCGGATTCCCCTCTGCAATTGGGGCAAAGGTTGCAAAACCAGATAGCAAAGTAATTTGTATAACTG
GTGATGGCGGATTTATGATGAACTGTCAAGAGCTCGGCACAATAGCCGAATACAATATCCCTGTTGTAATTTGTATATTT
GACAATAGAACCCTTGGAATGGTTTATCAATGGCAAAATTTATTCTATGGAAAAAGACAATGTAGTGTTAATTTCGGGGG
AGCTCCTGATTTTATAAAACTTGCAGAAAGCTACGGTATAAAAGCTAGAAGAATTGAATCACCTAATGAAATAAATGAAG
CACTTAAAGAAGCCATAAATTGTGATGAACCTTATTTATTAGATTTCGCAATTGACCCCTCATCAGCTCTTTCAATGGTG
CCACCAGGAGCAAAATTAACTAATATAATTGATGCAGTTCAAGAGCATCCAAATGAAAAAATCGTATGTTTCGATGAAAT
AAAGAGAAGATATATGGAAAATAAAAGAAATAGGAAATAA

Upstream 100 bases:

>100_bases
TTATATTATAATTTAATATATTTACTTATAATTATTATTATTATTATTTAACCATTATTAATTATTATTTAATTTATTAT
TTAATTTATTTGGTGATTTT

Downstream 100 bases:

>100_bases
AATAAAAAATATAATCTAAAATTTAATGGATTAATGGAAATGGCAAAACAAAGACGATAAATAAAATAATTTAATAAATT
AAATAGAAATGGGCCAATAG

Product: acetolactate synthase catalytic subunit

Products: NA

Alternate protein names: AHAS; Acetohydroxy-acid synthase large subunit; ALS [H]

Number of amino acids: Translated: 599; Mature: 599

Protein sequence:

>599_residues
MNGAEAMIKALEAEKVEILFGYPGGALLPFYDALHHSDLIHLLTRHEQAAAHAADGYARASGKVGVCIGTSGPGATNLVT
GVATAHSDSSPMVALTGQVPTKLIGNDAFQEIDALGLFMPIVKHNFQIQKTCQIPEIFRSAFEIAQTGRPGPVHIDLPKD
VQELELDIDKHPIPSKVKLIGYNPTTIGHPRQIKKAIKLIASAKRPIILAGGGVLLSGANEELLKLVELLNIPVCTTLMG
KGCISENHPLALGMVGMHGTKPANYCLSESDVLISIGCRFSDRITGDIKSFATNAKIIHIDIDPAEIGKNVAVDVPIVGD
AKLILKELIKQLDYIINKDSKENNDKENISQWIENVNSLKKSSIPVMDYDDIPIKPQKIVKELMAVIDDLNINKNTIITT
DVGQNQMWMAHYFKTQTPRSFLSSGGLGTMGFGFPSAIGAKVAKPDSKVICITGDGGFMMNCQELGTIAEYNIPVVICIF
DNRTLGMVYQWQNLFYGKRQCSVNFGGAPDFIKLAESYGIKARRIESPNEINEALKEAINCDEPYLLDFAIDPSSALSMV
PPGAKLTNIIDAVQEHPNEKIVCFDEIKRRYMENKRNRK

Sequences:

>Translated_599_residues
MNGAEAMIKALEAEKVEILFGYPGGALLPFYDALHHSDLIHLLTRHEQAAAHAADGYARASGKVGVCIGTSGPGATNLVT
GVATAHSDSSPMVALTGQVPTKLIGNDAFQEIDALGLFMPIVKHNFQIQKTCQIPEIFRSAFEIAQTGRPGPVHIDLPKD
VQELELDIDKHPIPSKVKLIGYNPTTIGHPRQIKKAIKLIASAKRPIILAGGGVLLSGANEELLKLVELLNIPVCTTLMG
KGCISENHPLALGMVGMHGTKPANYCLSESDVLISIGCRFSDRITGDIKSFATNAKIIHIDIDPAEIGKNVAVDVPIVGD
AKLILKELIKQLDYIINKDSKENNDKENISQWIENVNSLKKSSIPVMDYDDIPIKPQKIVKELMAVIDDLNINKNTIITT
DVGQNQMWMAHYFKTQTPRSFLSSGGLGTMGFGFPSAIGAKVAKPDSKVICITGDGGFMMNCQELGTIAEYNIPVVICIF
DNRTLGMVYQWQNLFYGKRQCSVNFGGAPDFIKLAESYGIKARRIESPNEINEALKEAINCDEPYLLDFAIDPSSALSMV
PPGAKLTNIIDAVQEHPNEKIVCFDEIKRRYMENKRNRK
>Mature_599_residues
MNGAEAMIKALEAEKVEILFGYPGGALLPFYDALHHSDLIHLLTRHEQAAAHAADGYARASGKVGVCIGTSGPGATNLVT
GVATAHSDSSPMVALTGQVPTKLIGNDAFQEIDALGLFMPIVKHNFQIQKTCQIPEIFRSAFEIAQTGRPGPVHIDLPKD
VQELELDIDKHPIPSKVKLIGYNPTTIGHPRQIKKAIKLIASAKRPIILAGGGVLLSGANEELLKLVELLNIPVCTTLMG
KGCISENHPLALGMVGMHGTKPANYCLSESDVLISIGCRFSDRITGDIKSFATNAKIIHIDIDPAEIGKNVAVDVPIVGD
AKLILKELIKQLDYIINKDSKENNDKENISQWIENVNSLKKSSIPVMDYDDIPIKPQKIVKELMAVIDDLNINKNTIITT
DVGQNQMWMAHYFKTQTPRSFLSSGGLGTMGFGFPSAIGAKVAKPDSKVICITGDGGFMMNCQELGTIAEYNIPVVICIF
DNRTLGMVYQWQNLFYGKRQCSVNFGGAPDFIKLAESYGIKARRIESPNEINEALKEAINCDEPYLLDFAIDPSSALSMV
PPGAKLTNIIDAVQEHPNEKIVCFDEIKRRYMENKRNRK

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI21361361, Length=571, Percent_Identity=26.4448336252189, Blast_Score=189, Evalue=9e-48,
Organism=Homo sapiens, GI93004078, Length=576, Percent_Identity=23.9583333333333, Blast_Score=183, Evalue=4e-46,
Organism=Escherichia coli, GI87081685, Length=562, Percent_Identity=43.5943060498221, Blast_Score=496, Evalue=1e-141,
Organism=Escherichia coli, GI1790104, Length=573, Percent_Identity=41.1867364746946, Blast_Score=438, Evalue=1e-124,
Organism=Escherichia coli, GI1786717, Length=590, Percent_Identity=32.3728813559322, Blast_Score=339, Evalue=2e-94,
Organism=Escherichia coli, GI1787096, Length=571, Percent_Identity=28.5464098073555, Blast_Score=221, Evalue=9e-59,
Organism=Escherichia coli, GI1788716, Length=570, Percent_Identity=26.6666666666667, Blast_Score=191, Evalue=2e-49,
Organism=Caenorhabditis elegans, GI17531299, Length=571, Percent_Identity=27.84588441331, Blast_Score=169, Evalue=3e-42,
Organism=Caenorhabditis elegans, GI17531301, Length=571, Percent_Identity=27.84588441331, Blast_Score=169, Evalue=4e-42,
Organism=Caenorhabditis elegans, GI17542570, Length=526, Percent_Identity=26.9961977186312, Blast_Score=165, Evalue=8e-41,
Organism=Saccharomyces cerevisiae, GI6323755, Length=621, Percent_Identity=40.0966183574879, Blast_Score=426, Evalue=1e-120,
Organism=Saccharomyces cerevisiae, GI6320816, Length=486, Percent_Identity=26.1316872427983, Blast_Score=124, Evalue=6e-29,
Organism=Saccharomyces cerevisiae, GI6320588, Length=568, Percent_Identity=23.7676056338028, Blast_Score=93, Evalue=1e-19,
Organism=Saccharomyces cerevisiae, GI6321524, Length=505, Percent_Identity=21.7821782178218, Blast_Score=91, Evalue=7e-19,
Organism=Saccharomyces cerevisiae, GI6323163, Length=483, Percent_Identity=24.223602484472, Blast_Score=87, Evalue=8e-18,
Organism=Saccharomyces cerevisiae, GI6323073, Length=558, Percent_Identity=21.6845878136201, Blast_Score=86, Evalue=2e-17,
Organism=Drosophila melanogaster, GI19922626, Length=568, Percent_Identity=25.1760563380282, Blast_Score=175, Evalue=1e-43,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012846
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 65396; Mature: 65396

Theoretical pI: Translated: 6.67; Mature: 6.67

Prosite motif: PS00187 TPP_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNGAEAMIKALEAEKVEILFGYPGGALLPFYDALHHSDLIHLLTRHEQAAAHAADGYARA
CCHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHCC
SGKVGVCIGTSGPGATNLVTGVATAHSDSSPMVALTGQVPTKLIGNDAFQEIDALGLFMP
CCCEEEEEECCCCCHHHHHHHEEECCCCCCCEEEEECCCCHHHHCCHHHHHHHHHHHHHH
IVKHNFQIQKTCQIPEIFRSAFEIAQTGRPGPVHIDLPKDVQELELDIDKHPIPSKVKLI
HHHCCCEEEECCCCHHHHHHHHHHHHCCCCCCEEEECCCCHHHHHHCCCCCCCCCCEEEE
GYNPTTIGHPRQIKKAIKLIASAKRPIILAGGGVLLSGANEELLKLVELLNIPVCTTLMG
ECCCCCCCCHHHHHHHHHHHHCCCCCEEEECCCEEEECCCHHHHHHHHHHCCCHHHHHHC
KGCISENHPLALGMVGMHGTKPANYCLSESDVLISIGCRFSDRITGDIKSFATNAKIIHI
CCCCCCCCCEEEEEECCCCCCCHHHCCCCCCEEEEECCCCCCCCCHHHHHHCCCCEEEEE
DIDPAEIGKNVAVDVPIVGDAKLILKELIKQLDYIINKDSKENNDKENISQWIENVNSLK
ECCHHHHCCCEEEEEEECCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHH
KSSIPVMDYDDIPIKPQKIVKELMAVIDDLNINKNTIITTDVGQNQMWMAHYFKTQTPRS
HHCCCEECCCCCCCCHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCEEHHHHHHCCCCHH
FLSSGGLGTMGFGFPSAIGAKVAKPDSKVICITGDGGFMMNCQELGTIAEYNIPVVICIF
HHHCCCCCCCCCCCHHHHCCCCCCCCCCEEEEECCCCCEEEHHHHCCEEECCCCEEEEEE
DNRTLGMVYQWQNLFYGKRQCSVNFGGAPDFIKLAESYGIKARRIESPNEINEALKEAIN
CCCCEEEEEEECHHHCCCEEEEEECCCCCHHHHHHHHCCCEEEECCCHHHHHHHHHHHHC
CDEPYLLDFAIDPSSALSMVPPGAKLTNIIDAVQEHPNEKIVCFDEIKRRYMENKRNRK
CCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MNGAEAMIKALEAEKVEILFGYPGGALLPFYDALHHSDLIHLLTRHEQAAAHAADGYARA
CCHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHCC
SGKVGVCIGTSGPGATNLVTGVATAHSDSSPMVALTGQVPTKLIGNDAFQEIDALGLFMP
CCCEEEEEECCCCCHHHHHHHEEECCCCCCCEEEEECCCCHHHHCCHHHHHHHHHHHHHH
IVKHNFQIQKTCQIPEIFRSAFEIAQTGRPGPVHIDLPKDVQELELDIDKHPIPSKVKLI
HHHCCCEEEECCCCHHHHHHHHHHHHCCCCCCEEEECCCCHHHHHHCCCCCCCCCCEEEE
GYNPTTIGHPRQIKKAIKLIASAKRPIILAGGGVLLSGANEELLKLVELLNIPVCTTLMG
ECCCCCCCCHHHHHHHHHHHHCCCCCEEEECCCEEEECCCHHHHHHHHHHCCCHHHHHHC
KGCISENHPLALGMVGMHGTKPANYCLSESDVLISIGCRFSDRITGDIKSFATNAKIIHI
CCCCCCCCCEEEEEECCCCCCCHHHCCCCCCEEEEECCCCCCCCCHHHHHHCCCCEEEEE
DIDPAEIGKNVAVDVPIVGDAKLILKELIKQLDYIINKDSKENNDKENISQWIENVNSLK
ECCHHHHCCCEEEEEEECCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHH
KSSIPVMDYDDIPIKPQKIVKELMAVIDDLNINKNTIITTDVGQNQMWMAHYFKTQTPRS
HHCCCEECCCCCCCCHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCEEHHHHHHCCCCHH
FLSSGGLGTMGFGFPSAIGAKVAKPDSKVICITGDGGFMMNCQELGTIAEYNIPVVICIF
HHHCCCCCCCCCCCHHHHCCCCCCCCCCEEEEECCCCCEEEHHHHCCEEECCCCEEEEEE
DNRTLGMVYQWQNLFYGKRQCSVNFGGAPDFIKLAESYGIKARRIESPNEINEALKEAIN
CCCCEEEEEEECHHHCCCEEEEEECCCCCHHHHHHHHCCCEEEECCCHHHHHHHHHHHHC
CDEPYLLDFAIDPSSALSMVPPGAKLTNIIDAVQEHPNEKIVCFDEIKRRYMENKRNRK
CCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9099862 [H]