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The map label for this gene is mtbA [H]

Identifier: 150401029

GI number: 150401029

Start: 612672

End: 613679

Strand: Direct

Name: mtbA [H]

Synonym: Maeo_0598

Alternate gene names: 150401029

Gene position: 612672-613679 (Clockwise)

Preceding gene: 150401028

Following gene: 150401030

Centisome position: 39.04

GC content: 36.01

Gene sequence:

>1008_bases
ATGACTCCAAAAGAAAGACTTTTAGGGGCAATTACTGGAAGCGGTATTGATAGAATACCTGCGGCCCCACTTATTGGAAA
CTTTGCTGGAAGAATGGCAGGGTATACCGTAAATGAAATTGCAAAAGATGGAACTAAATTGGCTAAATCCTTAGAATACA
CATACAACATGATTAAACCAGATTTGGTAACAATATTAACGGATTTAAGTATAGAAGCTGAGGCCATGGGTTGTGAATTG
GAGTATTTTGATAATGATGTCCCAGTAGTTAAAAGGCATGTATCACTCGATGAGATATCCATTCCGAATCCTGAAAAAGA
CGGAAGAATGCCCGAATTTTTAAAATGTATTGAGCATCTTGTGGAAAAAATTGGAAATGAAGTATTTATTGGAATGTGTG
TCCCAGGACCATTTACAATAACGGGAAATGTTCACGGAGCAGAGAGTATGATGATGGATTTGGTAATGAATCCAGATGTT
GTTAAAGAGGAAATAAAACTGGCAACAGAAACCTCAAAATTATTTATAAATGCATGCATGGATAGGGGAGCGATACCTTT
AATTTGTGATCCAATAGCATCTGGTTCATTAATAAGCCCGAAATTTTATGAGGAGTTTGCACTAAATCCCTCCAAAGAGA
TTGTAGATGAAATACACAAACGTGGATTACCAGCAGTATTGCATATTTGTGGGGACTCAAATCCAATACATGACCTTATG
GCTGAAACCGGGGCAGATTTTTTAAGTGTTGATGCCAATGTTAATTTAAAAGAAATGAAAGAGAAAGTAGGTAAAAAAGT
AGGTATAATTGGAAATATAGATCCTGGAAGCACCATATACCTTGGAAGTAAGGAAGAAGTCATTAATGAATGCAAAAAGG
CAATAGAATACGCATCAGACAATCCAAAAGGATTTATTCTTGCATCGGGTTGTGAAATACCATTAGAATCTAAGAAAGAA
AATGTAAAGGCAATAGTTGAAGCATCAGAAGTCTATGGAAAAATCTAG

Upstream 100 bases:

>100_bases
TAGGTGCAGATGCATATACTGAAAATGCTAACGAAGCGTTAAATGTTGCCAAAGAACTCTATAAAAAACGAAATAATGGA
CAATAAATAGGTGAAAAAAA

Downstream 100 bases:

>100_bases
TAAAAATCCGATAAATATTAAGATGTTATTTAACAATACCAACAATTTTAACCATATTTTTTTAAAGATGGATTGGGTGT
TTTTATGACGCCAAAAGAAA

Product: uroporphyrinogen decarboxylase

Products: CO2; Coproporphyrinogen III [C]

Alternate protein names: MT2-A; Methylcobamide:CoM methyltransferase II isozyme A [H]

Number of amino acids: Translated: 335; Mature: 334

Protein sequence:

>335_residues
MTPKERLLGAITGSGIDRIPAAPLIGNFAGRMAGYTVNEIAKDGTKLAKSLEYTYNMIKPDLVTILTDLSIEAEAMGCEL
EYFDNDVPVVKRHVSLDEISIPNPEKDGRMPEFLKCIEHLVEKIGNEVFIGMCVPGPFTITGNVHGAESMMMDLVMNPDV
VKEEIKLATETSKLFINACMDRGAIPLICDPIASGSLISPKFYEEFALNPSKEIVDEIHKRGLPAVLHICGDSNPIHDLM
AETGADFLSVDANVNLKEMKEKVGKKVGIIGNIDPGSTIYLGSKEEVINECKKAIEYASDNPKGFILASGCEIPLESKKE
NVKAIVEASEVYGKI

Sequences:

>Translated_335_residues
MTPKERLLGAITGSGIDRIPAAPLIGNFAGRMAGYTVNEIAKDGTKLAKSLEYTYNMIKPDLVTILTDLSIEAEAMGCEL
EYFDNDVPVVKRHVSLDEISIPNPEKDGRMPEFLKCIEHLVEKIGNEVFIGMCVPGPFTITGNVHGAESMMMDLVMNPDV
VKEEIKLATETSKLFINACMDRGAIPLICDPIASGSLISPKFYEEFALNPSKEIVDEIHKRGLPAVLHICGDSNPIHDLM
AETGADFLSVDANVNLKEMKEKVGKKVGIIGNIDPGSTIYLGSKEEVINECKKAIEYASDNPKGFILASGCEIPLESKKE
NVKAIVEASEVYGKI
>Mature_334_residues
TPKERLLGAITGSGIDRIPAAPLIGNFAGRMAGYTVNEIAKDGTKLAKSLEYTYNMIKPDLVTILTDLSIEAEAMGCELE
YFDNDVPVVKRHVSLDEISIPNPEKDGRMPEFLKCIEHLVEKIGNEVFIGMCVPGPFTITGNVHGAESMMMDLVMNPDVV
KEEIKLATETSKLFINACMDRGAIPLICDPIASGSLISPKFYEEFALNPSKEIVDEIHKRGLPAVLHICGDSNPIHDLMA
ETGADFLSVDANVNLKEMKEKVGKKVGIIGNIDPGSTIYLGSKEEVINECKKAIEYASDNPKGFILASGCEIPLESKKEN
VKAIVEASEVYGKI

Specific function: Catalyzes the transfer of the methyl group from the methylated corrinoid cofactor of mtmC, mtbC and mttC to coenzyme M [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the uroporphyrinogen decarboxylase family. MtbA/mtaA subfamily [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006360
- InterPro:   IPR000257 [H]

Pfam domain/function: PF01208 URO-D [H]

EC number: 4.1.1.37 [C]

Molecular weight: Translated: 36446; Mature: 36314

Theoretical pI: Translated: 4.61; Mature: 4.61

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
6.3 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPKERLLGAITGSGIDRIPAAPLIGNFAGRMAGYTVNEIAKDGTKLAKSLEYTYNMIKP
CCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCH
DLVTILTDLSIEAEAMGCELEYFDNDVPVVKRHVSLDEISIPNPEKDGRMPEFLKCIEHL
HHHHHHHCCCEEHHHHCCEEEECCCCCHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHH
VEKIGNEVFIGMCVPGPFTITGNVHGAESMMMDLVMNPDVVKEEIKLATETSKLFINACM
HHHCCCEEEEEEECCCCEEEECCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
DRGAIPLICDPIASGSLISPKFYEEFALNPSKEIVDEIHKRGLPAVLHICGDSNPIHDLM
CCCCCCEEECCCCCCCCCCCHHHHHHCCCCHHHHHHHHHHCCCCEEEEEECCCCHHHHHH
AETGADFLSVDANVNLKEMKEKVGKKVGIIGNIDPGSTIYLGSKEEVINECKKAIEYASD
HHCCCCEEEECCCCCHHHHHHHHCCCCCEEECCCCCCEEEECCHHHHHHHHHHHHHHCCC
NPKGFILASGCEIPLESKKENVKAIVEASEVYGKI
CCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TPKERLLGAITGSGIDRIPAAPLIGNFAGRMAGYTVNEIAKDGTKLAKSLEYTYNMIKP
CCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCH
DLVTILTDLSIEAEAMGCELEYFDNDVPVVKRHVSLDEISIPNPEKDGRMPEFLKCIEHL
HHHHHHHCCCEEHHHHCCEEEECCCCCHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHH
VEKIGNEVFIGMCVPGPFTITGNVHGAESMMMDLVMNPDVVKEEIKLATETSKLFINACM
HHHCCCEEEEEEECCCCEEEECCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
DRGAIPLICDPIASGSLISPKFYEEFALNPSKEIVDEIHKRGLPAVLHICGDSNPIHDLM
CCCCCCEEECCCCCCCCCCCHHHHHHCCCCHHHHHHHHHHCCCCEEEEEECCCCHHHHHH
AETGADFLSVDANVNLKEMKEKVGKKVGIIGNIDPGSTIYLGSKEEVINECKKAIEYASD
HHCCCCEEEECCCCCHHHHHHHHCCCCCEEECCCCCCEEEECCHHHHHHHHHHHHHHCCC
NPKGFILASGCEIPLESKKENVKAIVEASEVYGKI
CCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: Reducing agents [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.00085 {7-COOH-porphyrinogen} 0.00035 {7-COOH-porphyrinogen} 0.00231 {7-COOH,} 0.00533 {uroporphyrinogen} 0.00256 {uroporphyrinogen} 0.00133 {uroporphyrinogen} 0.001 {uroporphyrinogen} 0.0008 {uroporphyrinogen} 0.0004 {uroporphyrino

Substrates: H+; Uroporphyrinogen III [C]

Specific reaction: (4) H+ + Uroporphyrinogen III --> (4) CO2 + Coproporphyrinogen III [C]

General reaction: Decarboxylation [C]

Inhibitor: 5, 5'-Dithiobis (2-nitrobenzoate); 7-COOH-porphyrinogenI; 7-COOH-porphyrinogen III; Butanedione; Diethyl dicarbonate; Fe2+; Iodoacetamide; p-Hydroxy mercuribenzoate; Phenyl glyoxal; Pt2+; Sn2+; UroporphyrinogenI uroporphyrinogen III; Uroporphyrinogen III;

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11932238 [H]