Click here to switch to the map view.

The map label for this gene is mtbC1 [H]

Identifier: 150401028

GI number: 150401028

Start: 612001

End: 612657

Strand: Direct

Name: mtbC1 [H]

Synonym: Maeo_0597

Alternate gene names: 150401028

Gene position: 612001-612657 (Clockwise)

Preceding gene: 150401027

Following gene: 150401029

Centisome position: 38.99

GC content: 34.09

Gene sequence:

>657_bases
ATGAGTGCTGAAATTTTAAATAAACTTGCAGAATCTGTTGTAAATCTAGATGAAAATGGAGCCTTGAATGCAGCGCATGA
TGCATTAAAAACTGGAATTCCTGCACAGGTGGCCATAATTGAAGGGTTGTGTGAAGGCATGAAATTAGTCGGTAAGAAAT
ATGAAGAAAAAGAGTATTTCGTTCCAGAAGTATTAATGGCCTCAAATGCCATGAATAAAGCCGTAGATGTCTTAAAACCA
CATGTAATAATAGATGAAGACGATATTCCTGCTAAGATTGTAATGGGCGTAATTCAAGGAGATATTCATGAAATAGGAAA
GAATATTGTAGCAATGATGACAGAAGCAGGAGGTTTTGAAATTCATGATTTAGGTAGAAATGTACAGTGTGAAAAATTCG
TAGATTATGCCATAGAAAATAACGCAGATATAATCATGATGTCTACAATGATGACAACCACAATGTACAATATGAAAAAA
GTTATTGGTATTCTGGAAGAAAAAGGTGTTAGGGATAAATTTATAGTAATGATAGGTGGAGGTCCGGTATCTTCTGCTTT
TGCAAAAGAAATAGGTGCAGATGCATATACTGAAAATGCTAACGAAGCGTTAAATGTTGCCAAAGAACTCTATAAAAAAC
GAAATAATGGACAATAA

Upstream 100 bases:

>100_bases
CATTAAAATATGTAAAAAGAAAAATATATATAAAATAAAATAACAATATTACCAAAGATATAATTATATAGTACATTAAA
AAATCTAATAGGGCGATAGA

Downstream 100 bases:

>100_bases
ATAGGTGAAAAAAAATGACTCCAAAAGAAAGACTTTTAGGGGCAATTACTGGAAGCGGTATTGATAGAATACCTGCGGCC
CCACTTATTGGAAACTTTGC

Product: cobalamin B12-binding domain-containing protein

Products: L-Methionine; 5,6,7,8-Tetrahydrofolate [C]

Alternate protein names: NA

Number of amino acids: Translated: 218; Mature: 217

Protein sequence:

>218_residues
MSAEILNKLAESVVNLDENGALNAAHDALKTGIPAQVAIIEGLCEGMKLVGKKYEEKEYFVPEVLMASNAMNKAVDVLKP
HVIIDEDDIPAKIVMGVIQGDIHEIGKNIVAMMTEAGGFEIHDLGRNVQCEKFVDYAIENNADIIMMSTMMTTTMYNMKK
VIGILEEKGVRDKFIVMIGGGPVSSAFAKEIGADAYTENANEALNVAKELYKKRNNGQ

Sequences:

>Translated_218_residues
MSAEILNKLAESVVNLDENGALNAAHDALKTGIPAQVAIIEGLCEGMKLVGKKYEEKEYFVPEVLMASNAMNKAVDVLKP
HVIIDEDDIPAKIVMGVIQGDIHEIGKNIVAMMTEAGGFEIHDLGRNVQCEKFVDYAIENNADIIMMSTMMTTTMYNMKK
VIGILEEKGVRDKFIVMIGGGPVSSAFAKEIGADAYTENANEALNVAKELYKKRNNGQ
>Mature_217_residues
SAEILNKLAESVVNLDENGALNAAHDALKTGIPAQVAIIEGLCEGMKLVGKKYEEKEYFVPEVLMASNAMNKAVDVLKPH
VIIDEDDIPAKIVMGVIQGDIHEIGKNIVAMMTEAGGFEIHDLGRNVQCEKFVDYAIENNADIIMMSTMMTTTMYNMKKV
IGILEEKGVRDKFIVMIGGGPVSSAFAKEIGADAYTENANEALNVAKELYKKRNNGQ

Specific function: Acts as a methyl group carrier between mtbB and mtbA [H]

COG id: COG5012

COG function: function code R; Predicted cobalamin binding protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 B12-binding N-terminal domain [H]

Homologues:

Organism=Homo sapiens, GI169790923, Length=191, Percent_Identity=30.3664921465969, Blast_Score=85, Evalue=4e-17,
Organism=Escherichia coli, GI1790450, Length=183, Percent_Identity=32.2404371584699, Blast_Score=89, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17535405, Length=165, Percent_Identity=31.5151515151515, Blast_Score=77, Evalue=6e-15,

Paralogues:

None

Copy number: 513 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 1320 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006158
- InterPro:   IPR003759
- InterPro:   IPR012741 [H]

Pfam domain/function: PF02310 B12-binding; PF02607 B12-binding_2 [H]

EC number: 2.1.1.13 [C]

Molecular weight: Translated: 23768; Mature: 23637

Theoretical pI: Translated: 4.61; Mature: 4.61

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
6.4 %Met     (Translated Protein)
7.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
6.0 %Met     (Mature Protein)
6.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAEILNKLAESVVNLDENGALNAAHDALKTGIPAQVAIIEGLCEGMKLVGKKYEEKEYF
CCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCHHCCCHHH
VPEVLMASNAMNKAVDVLKPHVIIDEDDIPAKIVMGVIQGDIHEIGKNIVAMMTEAGGFE
HHHHHHHHHHHHHHHHHHCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEE
IHDLGRNVQCEKFVDYAIENNADIIMMSTMMTTTMYNMKKVIGILEEKGVRDKFIVMIGG
EECCCCCCCHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECC
GPVSSAFAKEIGADAYTENANEALNVAKELYKKRNNGQ
CCCHHHHHHHHCCCHHHCCHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
SAEILNKLAESVVNLDENGALNAAHDALKTGIPAQVAIIEGLCEGMKLVGKKYEEKEYF
CHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCHHCCCHHH
VPEVLMASNAMNKAVDVLKPHVIIDEDDIPAKIVMGVIQGDIHEIGKNIVAMMTEAGGFE
HHHHHHHHHHHHHHHHHHCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEE
IHDLGRNVQCEKFVDYAIENNADIIMMSTMMTTTMYNMKKVIGILEEKGVRDKFIVMIGG
EECCCCCCCHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECC
GPVSSAFAKEIGADAYTENANEALNVAKELYKKRNNGQ
CCCHHHHHHHHCCCHHHCCHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: ATP; Cobinamide; S-adenosyl-L-methionine [C]

Metal ions: Mg2+ [C]

Kcat value (1/min): 500-780 [C]

Specific activity: 3.8

Km value (mM): 0.0016 {S-adenosyl-L-methionine}} 0.00065 {S-adenosyl-L-methionine}} 0.06 {methyltetrahydrofolate}} 0.03 {methyltetrahydrofolate}} 0.016 {homocysteine}} 0.035 {S-methyltetrahydrofolate}} [C]

Substrates: 5-Methyltetrahydrofolate; L-Homocysteine [C]

Specific reaction: 5-Methyltetrahydrofolate + L-Homocysteine --> L-Methionine + 5,6,7,8-Tetrahydrofolate [C]

General reaction: Methyl group transfer [C]

Inhibitor: 5, 6-Dimethyl benzimidazole; alpha-Ribazole 3'-phosphate; Be2+; Cobinamide; Propyl iodide; Sr2+ [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12125824 [H]