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The map label for this gene is prf1 [H]

Identifier: 150400869

GI number: 150400869

Start: 456217

End: 457476

Strand: Reverse

Name: prf1 [H]

Synonym: Maeo_0437

Alternate gene names: 150400869

Gene position: 457476-456217 (Counterclockwise)

Preceding gene: 150400870

Following gene: 150400867

Centisome position: 29.15

GC content: 33.41

Gene sequence:

>1260_bases
ATGAGTAATTCCTCAACAGAGACATATTTATTCAAAAAAGCTCTAAAAGAATTAAAAGATAAAAAAGGAAAAGGGACAGA
ATTAATTAGTTTGTATATTCCGAAAGGAAAGAGAATTTCAGATGTAAGTCAGCATTTAAGGGAAGAAATATCCCAATCAG
CTAACATCAAAAGTAAAACCACTAAAAAAAATGTCCAGTCGGCAATTGAAGTAATATTGCAAAGATTAAAATTATTAAAA
GAACCACTTGAAAAAGGAGTAATTATATTTGCAGGAATGATTCCAAGAGGTGGTCCAGGAACAGAAAAAATGGAGACCTA
TGTTTTAGAACCGCCTGAACCTGTGAAAACATATATTTATAGATGCGATTCTCAATTTTATACCGAACCATTGGAAGACT
TTATGGCAGATAAAGATATTTATGGAGTAATTCTTGTAGATAGAAATGAAGCCACAATCGGAATAGTAAAAGGAAACAGC
ATATCCATTGTTAAAAGATTAACAAGTGGAGTTCCAGGTAAGTTTAAAGCAGGAGGGCAGTCAGCTCGAAGGTTGGAGCG
ATTAATTGATGATGCTGCACACCATTTTATGGTTAGAATTGGAGAATATTCCAATGAGATATTCCTTCCATTATTGGAGG
AGAAAAAATTAAGCGGATTATTGTTGGGCGGTCCTGGAAATACAAAAAATGAATTTGCAGAAAAAGGATATTTACACCAC
CAGTTAAAGCAAAAAATAATTGAAACTTTTGATTTATGTTATACGGAAGAAAGTGGTGTCAGGGAGCTCCTTGATAAAGC
TTCAACTTTATTGAGGGATTTAGATTTAATGAAGGAAAAAGAGGTAGTTCAGAGGTTCTTTAAGGAGCTTGTAAAAGAGG
ACGGAGGACTTGCCGTATATGGTGAAAAAGAGGTAATGAAATATCTTGAAATGGGGGCAGTTGATACTCTTATTATCACA
GAAGGCATCAATACTTCGCGAATTACTATAAAATGTAATAATTGTGAATATGTTGAAGAAAAAACCGTAAAAAATGAGGA
CATATTGAAATTAGAAGAAATATTAAAAACAAAAACCTGTCCCCGCTGTGAGGGGGCACTATATATTGACAGCGAAGAAG
ATATCATTGAATTCTATGCTAGATTATGTGATGAATCAAATTCAACCTTAAAAATAATATCTACTGATACAGAGGAAGGA
CATCAAATTTTAAAGGCTTTTAAGGGCGTTTGTGCCATTGTAAGATATAGGGTAGATTAA

Upstream 100 bases:

>100_bases
TTTTATAATAATATATTTTTATATTTTTATTAATATTTGGAGCTCCAAATATATCATATATTTTCAATTTTAAGAATAAT
AATATATTACAGGTGATTAA

Downstream 100 bases:

>100_bases
ATATACAAAATCCTAAAAAGATTTTGTATAAAATCCTAAAAAGATTTTGTTTAATAATAAGCCGGATAAGGAACAGTTAT
TGAAGATTAACTATATATTA

Product: peptide chain release factor 1

Products: GDP; phosphate

Alternate protein names: Translation termination factor aRF1 [H]

Number of amino acids: Translated: 419; Mature: 418

Protein sequence:

>419_residues
MSNSSTETYLFKKALKELKDKKGKGTELISLYIPKGKRISDVSQHLREEISQSANIKSKTTKKNVQSAIEVILQRLKLLK
EPLEKGVIIFAGMIPRGGPGTEKMETYVLEPPEPVKTYIYRCDSQFYTEPLEDFMADKDIYGVILVDRNEATIGIVKGNS
ISIVKRLTSGVPGKFKAGGQSARRLERLIDDAAHHFMVRIGEYSNEIFLPLLEEKKLSGLLLGGPGNTKNEFAEKGYLHH
QLKQKIIETFDLCYTEESGVRELLDKASTLLRDLDLMKEKEVVQRFFKELVKEDGGLAVYGEKEVMKYLEMGAVDTLIIT
EGINTSRITIKCNNCEYVEEKTVKNEDILKLEEILKTKTCPRCEGALYIDSEEDIIEFYARLCDESNSTLKIISTDTEEG
HQILKAFKGVCAIVRYRVD

Sequences:

>Translated_419_residues
MSNSSTETYLFKKALKELKDKKGKGTELISLYIPKGKRISDVSQHLREEISQSANIKSKTTKKNVQSAIEVILQRLKLLK
EPLEKGVIIFAGMIPRGGPGTEKMETYVLEPPEPVKTYIYRCDSQFYTEPLEDFMADKDIYGVILVDRNEATIGIVKGNS
ISIVKRLTSGVPGKFKAGGQSARRLERLIDDAAHHFMVRIGEYSNEIFLPLLEEKKLSGLLLGGPGNTKNEFAEKGYLHH
QLKQKIIETFDLCYTEESGVRELLDKASTLLRDLDLMKEKEVVQRFFKELVKEDGGLAVYGEKEVMKYLEMGAVDTLIIT
EGINTSRITIKCNNCEYVEEKTVKNEDILKLEEILKTKTCPRCEGALYIDSEEDIIEFYARLCDESNSTLKIISTDTEEG
HQILKAFKGVCAIVRYRVD
>Mature_418_residues
SNSSTETYLFKKALKELKDKKGKGTELISLYIPKGKRISDVSQHLREEISQSANIKSKTTKKNVQSAIEVILQRLKLLKE
PLEKGVIIFAGMIPRGGPGTEKMETYVLEPPEPVKTYIYRCDSQFYTEPLEDFMADKDIYGVILVDRNEATIGIVKGNSI
SIVKRLTSGVPGKFKAGGQSARRLERLIDDAAHHFMVRIGEYSNEIFLPLLEEKKLSGLLLGGPGNTKNEFAEKGYLHHQ
LKQKIIETFDLCYTEESGVRELLDKASTLLRDLDLMKEKEVVQRFFKELVKEDGGLAVYGEKEVMKYLEMGAVDTLIITE
GINTSRITIKCNNCEYVEEKTVKNEDILKLEEILKTKTCPRCEGALYIDSEEDIIEFYARLCDESNSTLKIISTDTEEGH
QILKAFKGVCAIVRYRVD

Specific function: Directs the termination of nascent peptide synthesis (translation) in response to the termination codons UAA, UAG and UGA [H]

COG id: COG1503

COG function: function code J; Peptide chain release factor 1 (eRF1)

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the eukaryotic release factor 1 family [H]

Homologues:

Organism=Homo sapiens, GI4759034, Length=423, Percent_Identity=30.9692671394799, Blast_Score=218, Evalue=6e-57,
Organism=Caenorhabditis elegans, GI72000368, Length=425, Percent_Identity=29.4117647058824, Blast_Score=207, Evalue=6e-54,
Organism=Saccharomyces cerevisiae, GI6319619, Length=417, Percent_Identity=29.4964028776978, Blast_Score=194, Evalue=2e-50,
Organism=Drosophila melanogaster, GI28574632, Length=419, Percent_Identity=29.5942720763723, Blast_Score=214, Evalue=1e-55,
Organism=Drosophila melanogaster, GI21356433, Length=419, Percent_Identity=29.5942720763723, Blast_Score=214, Evalue=1e-55,
Organism=Drosophila melanogaster, GI24667392, Length=419, Percent_Identity=29.5942720763723, Blast_Score=214, Evalue=1e-55,
Organism=Drosophila melanogaster, GI24667388, Length=419, Percent_Identity=29.5942720763723, Blast_Score=214, Evalue=1e-55,
Organism=Drosophila melanogaster, GI24667380, Length=419, Percent_Identity=29.5942720763723, Blast_Score=214, Evalue=1e-55,
Organism=Drosophila melanogaster, GI24667384, Length=419, Percent_Identity=29.5942720763723, Blast_Score=214, Evalue=1e-55,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005140
- InterPro:   IPR005141
- InterPro:   IPR005142
- InterPro:   IPR020918
- InterPro:   IPR004403 [H]

Pfam domain/function: PF03463 eRF1_1; PF03464 eRF1_2; PF03465 eRF1_3 [H]

EC number: 3.6.5.3

Molecular weight: Translated: 47431; Mature: 47300

Theoretical pI: Translated: 6.30; Mature: 6.30

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNSSTETYLFKKALKELKDKKGKGTELISLYIPKGKRISDVSQHLREEISQSANIKSKT
CCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHCCCCHHH
TKKNVQSAIEVILQRLKLLKEPLEKGVIIFAGMIPRGGPGTEKMETYVLEPPEPVKTYIY
HHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCCHHHEEECCCCCHHHHHHH
RCDSQFYTEPLEDFMADKDIYGVILVDRNEATIGIVKGNSISIVKRLTSGVPGKFKAGGQ
HCCCHHHHHHHHHHHCCCCEEEEEEEECCCCEEEEEECCCHHHHHHHHCCCCCCCCCCCH
SARRLERLIDDAAHHFMVRIGEYSNEIFLPLLEEKKLSGLLLGGPGNTKNEFAEKGYLHH
HHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEHHCCCCCEEEEECCCCCHHHHHHHHHHHH
QLKQKIIETFDLCYTEESGVRELLDKASTLLRDLDLMKEKEVVQRFFKELVKEDGGLAVY
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEE
GEKEVMKYLEMGAVDTLIITEGINTSRITIKCNNCEYVEEKTVKNEDILKLEEILKTKTC
CHHHHHHHHHHCCCCEEEEECCCCCEEEEEEECCCCHHHHHCCCCCHHHHHHHHHHHCCC
PRCEGALYIDSEEDIIEFYARLCDESNSTLKIISTDTEEGHQILKAFKGVCAIVRYRVD
CCCCCEEEECCCHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SNSSTETYLFKKALKELKDKKGKGTELISLYIPKGKRISDVSQHLREEISQSANIKSKT
CCCCHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHCCCCHHH
TKKNVQSAIEVILQRLKLLKEPLEKGVIIFAGMIPRGGPGTEKMETYVLEPPEPVKTYIY
HHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCCHHHEEECCCCCHHHHHHH
RCDSQFYTEPLEDFMADKDIYGVILVDRNEATIGIVKGNSISIVKRLTSGVPGKFKAGGQ
HCCCHHHHHHHHHHHCCCCEEEEEEEECCCCEEEEEECCCHHHHHHHHCCCCCCCCCCCH
SARRLERLIDDAAHHFMVRIGEYSNEIFLPLLEEKKLSGLLLGGPGNTKNEFAEKGYLHH
HHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEHHCCCCCEEEEECCCCCHHHHHHHHHHHH
QLKQKIIETFDLCYTEESGVRELLDKASTLLRDLDLMKEKEVVQRFFKELVKEDGGLAVY
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEE
GEKEVMKYLEMGAVDTLIITEGINTSRITIKCNNCEYVEEKTVKNEDILKLEEILKTKTC
CHHHHHHHHHHCCCCEEEEECCCCCEEEEEEECCCCHHHHHCCCCCHHHHHHHHHHHCCC
PRCEGALYIDSEEDIIEFYARLCDESNSTLKIISTDTEEGHQILKAFKGVCAIVRYRVD
CCCCCEEEECCCHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: GTP; H2O

Specific reaction: GTP + H2O = GDP + phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA