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The map label for this gene is dphB

Identifier: 150400867

GI number: 150400867

Start: 454675

End: 455448

Strand: Reverse

Name: dphB

Synonym: Maeo_0435

Alternate gene names: 150400867

Gene position: 455448-454675 (Counterclockwise)

Preceding gene: 150400869

Following gene: 150400866

Centisome position: 29.02

GC content: 30.49

Gene sequence:

>774_bases
ATGATTATATTGGCAGGATTAGGATTATATGATGAAAAAGACACAACACTAAAAACAGTTGAATTTGCTAAAAAGGCAGA
TAAAATATATGCAGAATTTTATACTGCTATTTTAACGGGCACCACGATAGAAAAAATAGAGGAAACACTTGGAAAAGAAA
TAACAATATTGGATAGGGAAAAAGTTGAGTTAGAAACAGAAAAGTTAATTAATGAAAGTAAAGATAAAGATATAATGTTT
CTTACAGCAGGAGACCCAATGGTGGCAACAACTCATATTGATATTGCAATTGAAGCTAAAAAGAAAGGAATTGAAGTAAT
TATATTAAATGCACCATCTATATATTCGGCAATTGGTATAACCGGATTACAATTATATAAATTTGGAAAAACCACTTCCA
TAGTATTTCCAGAGCCAAATTATTTCCCTGAAACCCCCTACGATGTTATAAAAGAAAACTCAAAAATGGGATATCATACG
CTATGTCTTTTGGATATTCAGGCACATAACACACGATTTATGACTGCAAACGAAGGACTTGAAGTATTATTGAAAATTGA
AGAAAAAAGAAATGAAAATATATTAAATAAAGATACAAAGGTTGTAGTTGTTGCAAGAGCGGGAAGTTTAAAACCTAAAA
TGGTATATGGAAAAATAGGGGATTTATTGGAATATGATTTCGGAGCTCCATTACATTGCATAATATTCACTGGAAATTTG
CACTTTATGGAGGAGGATGCTTTAAAATATTTATGCGAAAATATATCTGAATAG

Upstream 100 bases:

>100_bases
GATATTTGGAGAAATTATGTTTTCTCTTTACACATAACATTATGAAAAATGTTCGTCAATTTACTATAACAATTGAATTA
ATAAAAATTTAGGTGATATT

Downstream 100 bases:

>100_bases
ACGAACACTTTTCGTTAATTTAGGTATAAATATATTAAATATATATGCTCTGTATGTTAATCCTTACCCGTGAATCAGAG
TATTTTTTTGTTATTTCTGT

Product: diphthine synthase

Products: NA

Alternate protein names: Diphthamide biosynthesis methyltransferase

Number of amino acids: Translated: 257; Mature: 257

Protein sequence:

>257_residues
MIILAGLGLYDEKDTTLKTVEFAKKADKIYAEFYTAILTGTTIEKIEETLGKEITILDREKVELETEKLINESKDKDIMF
LTAGDPMVATTHIDIAIEAKKKGIEVIILNAPSIYSAIGITGLQLYKFGKTTSIVFPEPNYFPETPYDVIKENSKMGYHT
LCLLDIQAHNTRFMTANEGLEVLLKIEEKRNENILNKDTKVVVVARAGSLKPKMVYGKIGDLLEYDFGAPLHCIIFTGNL
HFMEEDALKYLCENISE

Sequences:

>Translated_257_residues
MIILAGLGLYDEKDTTLKTVEFAKKADKIYAEFYTAILTGTTIEKIEETLGKEITILDREKVELETEKLINESKDKDIMF
LTAGDPMVATTHIDIAIEAKKKGIEVIILNAPSIYSAIGITGLQLYKFGKTTSIVFPEPNYFPETPYDVIKENSKMGYHT
LCLLDIQAHNTRFMTANEGLEVLLKIEEKRNENILNKDTKVVVVARAGSLKPKMVYGKIGDLLEYDFGAPLHCIIFTGNL
HFMEEDALKYLCENISE
>Mature_257_residues
MIILAGLGLYDEKDTTLKTVEFAKKADKIYAEFYTAILTGTTIEKIEETLGKEITILDREKVELETEKLINESKDKDIMF
LTAGDPMVATTHIDIAIEAKKKGIEVIILNAPSIYSAIGITGLQLYKFGKTTSIVFPEPNYFPETPYDVIKENSKMGYHT
LCLLDIQAHNTRFMTANEGLEVLLKIEEKRNENILNKDTKVVVVARAGSLKPKMVYGKIGDLLEYDFGAPLHCIIFTGNL
HFMEEDALKYLCENISE

Specific function: Required for the methylation step in diphthamide biosynthesis

COG id: COG1798

COG function: function code J; Diphthamide biosynthesis methyltransferase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the diphthine synthase family

Homologues:

Organism=Homo sapiens, GI117190324, Length=278, Percent_Identity=37.7697841726619, Blast_Score=184, Evalue=6e-47,
Organism=Homo sapiens, GI117190326, Length=279, Percent_Identity=37.2759856630824, Blast_Score=182, Evalue=2e-46,
Organism=Homo sapiens, GI117190262, Length=279, Percent_Identity=37.2759856630824, Blast_Score=182, Evalue=2e-46,
Organism=Caenorhabditis elegans, GI17531369, Length=266, Percent_Identity=37.593984962406, Blast_Score=169, Evalue=1e-42,
Organism=Saccharomyces cerevisiae, GI6323201, Length=254, Percent_Identity=39.7637795275591, Blast_Score=191, Evalue=1e-49,
Organism=Drosophila melanogaster, GI28572120, Length=275, Percent_Identity=35.2727272727273, Blast_Score=164, Evalue=4e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DPHB_META3 (A6UU49)

Other databases:

- EMBL:   CP000743
- RefSeq:   YP_001324633.1
- ProteinModelPortal:   A6UU49
- SMR:   A6UU49
- STRING:   A6UU49
- GeneID:   5327287
- GenomeReviews:   CP000743_GR
- KEGG:   mae:Maeo_0435
- eggNOG:   arNOG04257
- HOGENOM:   HBG460511
- OMA:   YAEFYTA
- ProtClustDB:   PRK04160
- BioCyc:   MAEO419665:MAEO_0435-MONOMER
- HAMAP:   MF_01084
- InterPro:   IPR000878
- InterPro:   IPR014777
- InterPro:   IPR014776
- InterPro:   IPR004551
- Gene3D:   G3DSA:3.40.1010.10
- Gene3D:   G3DSA:3.30.950.10
- PANTHER:   PTHR10882
- PIRSF:   PIRSF036432
- TIGRFAMs:   TIGR00522

Pfam domain/function: PF00590 TP_methylase; SSF53790 Cor/por_Metransf

EC number: =2.1.1.98

Molecular weight: Translated: 28910; Mature: 28910

Theoretical pI: Translated: 4.80; Mature: 4.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIILAGLGLYDEKDTTLKTVEFAKKADKIYAEFYTAILTGTTIEKIEETLGKEITILDRE
CEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCEEEEEECC
KVELETEKLINESKDKDIMFLTAGDPMVATTHIDIAIEAKKKGIEVIILNAPSIYSAIGI
HHHHHHHHHHCCCCCCCEEEEECCCCEEEEEEEEEEEEECCCCEEEEEEECCCHHHHHCC
TGLQLYKFGKTTSIVFPEPNYFPETPYDVIKENSKMGYHTLCLLDIQAHNTRFMTANEGL
CEEEEEECCCEEEEEECCCCCCCCCCHHHHHCCCCCCEEEEEEEEEEECCEEEEECCCCE
EVLLKIEEKRNENILNKDTKVVVVARAGSLKPKMVYGKIGDLLEYDFGAPLHCIIFTGNL
EEEEEEEHHCCCCCCCCCCEEEEEEECCCCCCEEEECCHHHHHHHCCCCCEEEEEEECCE
HFMEEDALKYLCENISE
EEEHHHHHHHHHHHHCC
>Mature Secondary Structure
MIILAGLGLYDEKDTTLKTVEFAKKADKIYAEFYTAILTGTTIEKIEETLGKEITILDRE
CEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCEEEEEECC
KVELETEKLINESKDKDIMFLTAGDPMVATTHIDIAIEAKKKGIEVIILNAPSIYSAIGI
HHHHHHHHHHCCCCCCCEEEEECCCCEEEEEEEEEEEEECCCCEEEEEEECCCHHHHHCC
TGLQLYKFGKTTSIVFPEPNYFPETPYDVIKENSKMGYHTLCLLDIQAHNTRFMTANEGL
CEEEEEECCCEEEEEECCCCCCCCCCHHHHHCCCCCCEEEEEEEEEEECCEEEEECCCCE
EVLLKIEEKRNENILNKDTKVVVVARAGSLKPKMVYGKIGDLLEYDFGAPLHCIIFTGNL
EEEEEEEHHCCCCCCCCCCEEEEEEECCCCCCEEEECCHHHHHHHCCCCCEEEEEEECCE
HFMEEDALKYLCENISE
EEEHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA