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The map label for this gene is truA [H]

Identifier: 150400022

GI number: 150400022

Start: 1305366

End: 1306163

Strand: Reverse

Name: truA [H]

Synonym: Mevan_1280

Alternate gene names: 150400022

Gene position: 1306163-1305366 (Counterclockwise)

Preceding gene: 150400024

Following gene: 150400021

Centisome position: 75.94

GC content: 31.95

Gene sequence:

>798_bases
ATGTATATATTCAAAATTGCGTATGATGGACGTTACAGCTTTCAATCCCAACCCCATAGAAATACGGTATGTGATAAGAT
TACGGATACACTTTTAGAGTGCGGTTATTTAAAAGAAGGTGAAATACCTATCTCATATGGTGGAAGGACTGATTTAGGGG
TATCCGCACTTGGAAACTATATTGTTTACAATATAACTGAAAAACCAGTGCTTTCAAGAATTTACTCGAAATTACATAAA
CACGGAATCTGGATACTGGGTTTTAATGAACTAAAAGAGCTTCCCGATGTAAAATATCGACATTATCGATATATTCTCCC
AAATACGGGTCAAGACATTGAATTAATGAAAAAGGCATCGGAAAAATTAATTGGAACGCATTCTTTCCATAATCTATCAA
AAAGAGATAAAACTAAGAACAGAAGTCCCATAAGAACGATTTATAATATTAAAATTGCTAAAAATGAGTATTTTATAACC
ATTGAAATTTTTGGAAAGAGCTTTTTATGGAACATGGTTCGAAAGATTGTAAGGCTTCTTTCTAATGTTGGATTAAGCAA
ATACAAAGAGTTTGAAAAATTCATTGATGATTTATTGGATAGGGATGTAAGAATGGGAGTTCAACCTGCAAGTGCAGAAG
GTTTAATACTCGTCGATGTTAAAACGAATACTGAATTTAAAATTGATGAATATACGGTTAAAAAATTTGATCAGTATTTT
AAAAAGTCCCTAAACAACCATTCCATGAATATGGGTCTTTCAAAAACCATGCTATCCAAAAATCAGGTGAGTTTATGA

Upstream 100 bases:

>100_bases
TAATTGTGCAACATTAAAAAGTCCTCCAAAAATTTATATATATGAAAACCATACAAAAAAGGACACTTCGAAGCCAAAAT
TCCTAATTAATCGTGATAAA

Downstream 100 bases:

>100_bases
AGTATATTTCCTCAATAAGCGTAGATATCTCTTCAAATGACGTTGAAACTAATCCGGTCGTTAATGAAAAAATTGAGCGA
GAACTTCAAGTAGAATTTTC

Product: tRNA pseudouridine synthase A

Products: NA

Alternate protein names: tRNA pseudouridylate synthase I; tRNA-uridine isomerase I [H]

Number of amino acids: Translated: 265; Mature: 265

Protein sequence:

>265_residues
MYIFKIAYDGRYSFQSQPHRNTVCDKITDTLLECGYLKEGEIPISYGGRTDLGVSALGNYIVYNITEKPVLSRIYSKLHK
HGIWILGFNELKELPDVKYRHYRYILPNTGQDIELMKKASEKLIGTHSFHNLSKRDKTKNRSPIRTIYNIKIAKNEYFIT
IEIFGKSFLWNMVRKIVRLLSNVGLSKYKEFEKFIDDLLDRDVRMGVQPASAEGLILVDVKTNTEFKIDEYTVKKFDQYF
KKSLNNHSMNMGLSKTMLSKNQVSL

Sequences:

>Translated_265_residues
MYIFKIAYDGRYSFQSQPHRNTVCDKITDTLLECGYLKEGEIPISYGGRTDLGVSALGNYIVYNITEKPVLSRIYSKLHK
HGIWILGFNELKELPDVKYRHYRYILPNTGQDIELMKKASEKLIGTHSFHNLSKRDKTKNRSPIRTIYNIKIAKNEYFIT
IEIFGKSFLWNMVRKIVRLLSNVGLSKYKEFEKFIDDLLDRDVRMGVQPASAEGLILVDVKTNTEFKIDEYTVKKFDQYF
KKSLNNHSMNMGLSKTMLSKNQVSL
>Mature_265_residues
MYIFKIAYDGRYSFQSQPHRNTVCDKITDTLLECGYLKEGEIPISYGGRTDLGVSALGNYIVYNITEKPVLSRIYSKLHK
HGIWILGFNELKELPDVKYRHYRYILPNTGQDIELMKKASEKLIGTHSFHNLSKRDKTKNRSPIRTIYNIKIAKNEYFIT
IEIFGKSFLWNMVRKIVRLLSNVGLSKYKEFEKFIDDLLDRDVRMGVQPASAEGLILVDVKTNTEFKIDEYTVKKFDQYF
KKSLNNHSMNMGLSKTMLSKNQVSL

Specific function: Formation of pseudouridine at positions 38, 39 and 40 in the anticodon stem and loop of transfer RNAs [H]

COG id: COG0101

COG function: function code J; Pseudouridylate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA pseudouridine synthase truA family [H]

Homologues:

Organism=Escherichia coli, GI1788657, Length=258, Percent_Identity=27.1317829457364, Blast_Score=76, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI71985046, Length=294, Percent_Identity=24.4897959183673, Blast_Score=66, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR001406
- InterPro:   IPR020097
- InterPro:   IPR020095
- InterPro:   IPR020094 [H]

Pfam domain/function: PF01416 PseudoU_synth_1 [H]

EC number: =5.4.99.12 [H]

Molecular weight: Translated: 30877; Mature: 30877

Theoretical pI: Translated: 9.94; Mature: 9.94

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYIFKIAYDGRYSFQSQPHRNTVCDKITDTLLECGYLKEGEIPISYGGRTDLGVSALGNY
CEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCHHHHCCE
IVYNITEKPVLSRIYSKLHKHGIWILGFNELKELPDVKYRHYRYILPNTGQDIELMKKAS
EEEECCCHHHHHHHHHHHHHCCEEEEEHHHHHCCCCCCEEEEEEECCCCCCHHHHHHHHH
EKLIGTHSFHNLSKRDKTKNRSPIRTIYNIKIAKNEYFITIEIFGKSFLWNMVRKIVRLL
HHHHCCHHHHCCHHHHCCCCCCCCEEEEEEEEECCCEEEEEEECCHHHHHHHHHHHHHHH
SNVGLSKYKEFEKFIDDLLDRDVRMGVQPASAEGLILVDVKTNTEFKIDEYTVKKFDQYF
HHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCCEEEHHHHHHHHHHHH
KKSLNNHSMNMGLSKTMLSKNQVSL
HHHCCCCCCCCCHHHHHHCCCCCCC
>Mature Secondary Structure
MYIFKIAYDGRYSFQSQPHRNTVCDKITDTLLECGYLKEGEIPISYGGRTDLGVSALGNY
CEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCHHHHCCE
IVYNITEKPVLSRIYSKLHKHGIWILGFNELKELPDVKYRHYRYILPNTGQDIELMKKAS
EEEECCCHHHHHHHHHHHHHCCEEEEEHHHHHCCCCCCEEEEEEECCCCCCHHHHHHHHH
EKLIGTHSFHNLSKRDKTKNRSPIRTIYNIKIAKNEYFITIEIFGKSFLWNMVRKIVRLL
HHHHCCHHHHCCHHHHCCCCCCCCEEEEEEEEECCCEEEEEEECCHHHHHHHHHHHHHHH
SNVGLSKYKEFEKFIDDLLDRDVRMGVQPASAEGLILVDVKTNTEFKIDEYTVKKFDQYF
HHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCCEEEHHHHHHHHHHHH
KKSLNNHSMNMGLSKTMLSKNQVSL
HHHCCCCCCCCCHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA