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The map label for this gene is cobD

Identifier: 150398992

GI number: 150398992

Start: 281063

End: 281995

Strand: Reverse

Name: cobD

Synonym: Mevan_0238

Alternate gene names: 150398992

Gene position: 281995-281063 (Counterclockwise)

Preceding gene: 150398993

Following gene: 150398983

Centisome position: 16.39

GC content: 30.23

Gene sequence:

>933_bases
ATGATAAATCCAATTTATTTAATTATTGCAGACCTTTTAGATAGATACATTGGAGAGCCTCCTGAAAAACTCCATCCTGT
TGTTTTTATCGGGAATTTCGTTAAGTTTTTAGAAAAAGTGTTTCCATCAACGCATTCAGTTGAAAAGCTTAAAGATTTAG
TTTTTGGATTTATTACGGTATTTTTAACGGTTTTTACCGTATTTTTAATATTTTTTACGCTGGAATTAATTTTAAATTTA
ATTTCAAATTATTATATTAAATTATTTTCCTATTCTTTAATTCTCTCATTTTCTATTGGCCATAAATCACTTTTAGAATT
TTCAAAAGCCCCAATAAAGTACATAATGTCTGGGGATATTAAAAGCGCTAGAAAGTCAGTTCAGCATATCGTAAGTAGGG
ATACATCAACTTTAGATGAAAAGCACGTAATTTCAGCAGCAGTTGAAAGCTCTTCAGAAAATATTACGGACAGTATAATT
GCACCATTAATTTATGCTGCAATTTTTGGGCTTTCGGGTGCATTTATATACCGTGCAGTAAACACAATGGATGCAATGCT
AGGTTATAAAAACGAAAAATATCGATATTACGGAAAAACTGCCGCCTATTTAGATGATATTTTAAATTTTATCCCTTCAA
GAATTGCCGGAATTTTATTAATAATTTCTGCACCCTTTTACGGTGGAAAAATTGTTCCCGCCATCTATGGATATCTAAAA
GAAGGTTTTAAAACACCATCTCCAAATTCGGGATACACAATGGCAGTAATTGCAAACTCGCTTTCAATGGAACTTGAAAA
AATAGGTTGTTATAAACTTGGAAAAGGAGAAATTACTGTTTTAAAGGCAGTAAATTCACTAAAAGCAGTTGATTATTCGG
TATTACTATTTTTAGTAATTTACATGGTCTTATACTTTAATCTAATATATTAA

Upstream 100 bases:

>100_bases
AAATAGACTTAAAAGTTCTTGAAAAGTATTGTAAAATTAAAAAAGAAAAAACGAAAAGCTTTTTAAATGCCCTTGGAAAA
CTTTAAGAGACTTTGAAAAA

Downstream 100 bases:

>100_bases
TATAATACTCAATCGAATAATCTAAATTGTTTTAGAAATTTAAAAAAGTTTAATACGCATTTAAAAAAGTTTAAATTATC
ATTAAAAAAATACTTTTTAA

Product: cobalamin biosynthesis protein

Products: ADP; phosphate; Adenosyl cobinamide phosphate; Adenosyl cobinamide

Alternate protein names: NA

Number of amino acids: Translated: 310; Mature: 310

Protein sequence:

>310_residues
MINPIYLIIADLLDRYIGEPPEKLHPVVFIGNFVKFLEKVFPSTHSVEKLKDLVFGFITVFLTVFTVFLIFFTLELILNL
ISNYYIKLFSYSLILSFSIGHKSLLEFSKAPIKYIMSGDIKSARKSVQHIVSRDTSTLDEKHVISAAVESSSENITDSII
APLIYAAIFGLSGAFIYRAVNTMDAMLGYKNEKYRYYGKTAAYLDDILNFIPSRIAGILLIISAPFYGGKIVPAIYGYLK
EGFKTPSPNSGYTMAVIANSLSMELEKIGCYKLGKGEITVLKAVNSLKAVDYSVLLFLVIYMVLYFNLIY

Sequences:

>Translated_310_residues
MINPIYLIIADLLDRYIGEPPEKLHPVVFIGNFVKFLEKVFPSTHSVEKLKDLVFGFITVFLTVFTVFLIFFTLELILNL
ISNYYIKLFSYSLILSFSIGHKSLLEFSKAPIKYIMSGDIKSARKSVQHIVSRDTSTLDEKHVISAAVESSSENITDSII
APLIYAAIFGLSGAFIYRAVNTMDAMLGYKNEKYRYYGKTAAYLDDILNFIPSRIAGILLIISAPFYGGKIVPAIYGYLK
EGFKTPSPNSGYTMAVIANSLSMELEKIGCYKLGKGEITVLKAVNSLKAVDYSVLLFLVIYMVLYFNLIY
>Mature_310_residues
MINPIYLIIADLLDRYIGEPPEKLHPVVFIGNFVKFLEKVFPSTHSVEKLKDLVFGFITVFLTVFTVFLIFFTLELILNL
ISNYYIKLFSYSLILSFSIGHKSLLEFSKAPIKYIMSGDIKSARKSVQHIVSRDTSTLDEKHVISAAVESSSENITDSII
APLIYAAIFGLSGAFIYRAVNTMDAMLGYKNEKYRYYGKTAAYLDDILNFIPSRIAGILLIISAPFYGGKIVPAIYGYLK
EGFKTPSPNSGYTMAVIANSLSMELEKIGCYKLGKGEITVLKAVNSLKAVDYSVLLFLVIYMVLYFNLIY

Specific function: Converts cobyric acid to cobinamide by the addition of aminopropanol on the F carboxylic group

COG id: COG1270

COG function: function code H; Cobalamin biosynthesis protein CobD/CbiB

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential)

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CobD/CbiB family

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): COBD_METVS (A6UNS5)

Other databases:

- EMBL:   CP000742
- RefSeq:   YP_001322759.1
- STRING:   A6UNS5
- GeneID:   5324961
- GenomeReviews:   CP000742_GR
- KEGG:   mvn:Mevan_0238
- eggNOG:   arNOG05935
- HOGENOM:   HBG647131
- OMA:   MINPIYL
- ProtClustDB:   PRK01209
- HAMAP:   MF_00024
- InterPro:   IPR004485
- TIGRFAMs:   TIGR00380

Pfam domain/function: PF03186 CobD_Cbib

EC number: 6.3.1.10

Molecular weight: Translated: 34783; Mature: 34783

Theoretical pI: Translated: 9.10; Mature: 9.10

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x15d49e90)-; HASH(0x162f1c70)-; HASH(0x1712499c)-; HASH(0x162edb58)-; HASH(0x170f21f4)-;

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MINPIYLIIADLLDRYIGEPPEKLHPVVFIGNFVKFLEKVFPSTHSVEKLKDLVFGFITV
CCCHHHHHHHHHHHHHHCCCHHHHCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
FLTVFTVFLIFFTLELILNLISNYYIKLFSYSLILSFSIGHKSLLEFSKAPIKYIMSGDI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCHHHHHCCCH
KSARKSVQHIVSRDTSTLDEKHVISAAVESSSENITDSIIAPLIYAAIFGLSGAFIYRAV
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHH
NTMDAMLGYKNEKYRYYGKTAAYLDDILNFIPSRIAGILLIISAPFYGGKIVPAIYGYLK
HHHHHHHCCCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHH
EGFKTPSPNSGYTMAVIANSLSMELEKIGCYKLGKGEITVLKAVNSLKAVDYSVLLFLVI
HCCCCCCCCCCEEEEEEHHHHHHHHHHHCCEECCCCCCHHHHHHHHHHHHHHHHHHHHHH
YMVLYFNLIY
HHHHHHHHCH
>Mature Secondary Structure
MINPIYLIIADLLDRYIGEPPEKLHPVVFIGNFVKFLEKVFPSTHSVEKLKDLVFGFITV
CCCHHHHHHHHHHHHHHCCCHHHHCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
FLTVFTVFLIFFTLELILNLISNYYIKLFSYSLILSFSIGHKSLLEFSKAPIKYIMSGDI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCHHHHHCCCH
KSARKSVQHIVSRDTSTLDEKHVISAAVESSSENITDSIIAPLIYAAIFGLSGAFIYRAV
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHH
NTMDAMLGYKNEKYRYYGKTAAYLDDILNFIPSRIAGILLIISAPFYGGKIVPAIYGYLK
HHHHHHHCCCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHH
EGFKTPSPNSGYTMAVIANSLSMELEKIGCYKLGKGEITVLKAVNSLKAVDYSVLLFLVI
HCCCCCCCCCCEEEEEEHHHHHHHHHHHCCEECCCCCCHHHHHHHHHHHHHHHHHHHHHH
YMVLYFNLIY
HHHHHHHHCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; adenosylcobyric acid; (R)-1-aminopropan-2-yl phosphate; (R)-1-aminopropan-2-ol

Specific reaction: (1) ATP + adenosylcobyric acid + (R)-1-aminopropan-2-yl phosphate = ADP + phosphate + adenosylcobinamide phosphate; (2) ATP + adenosylcobyric acid + (R)-1-aminopropan-2-ol = ADP + phosphate + adenosylcobinamide

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA