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The map label for this gene is prfC [H]

Identifier: 150398738

GI number: 150398738

Start: 3759850

End: 3761433

Strand: Direct

Name: prfC [H]

Synonym: Smed_3555

Alternate gene names: 150398738

Gene position: 3759850-3761433 (Clockwise)

Preceding gene: 150398736

Following gene: 150398748

Centisome position: 99.42

GC content: 63.19

Gene sequence:

>1584_bases
ATGGCCGAAAGCATTGCCGAGGCGGTCTCCCGCCGCCGCACATTTGCGATCATTTCGCACCCGGACGCCGGTAAGACGAC
GCTCACTGAGAAGCTTCTGCTGTTCGGTGGCGCGATCCAGCTTGCCGGCGAGGTCAAGGCCAAGAAGGATCGCATCCAAA
CCCGTTCGGACTGGATGAAGATCGAGCGTGAGCGCGGCATTTCCGTCGTTACCTCGGTGATGACTTTCGAATACGACGAT
ACCGTATTCAACCTCCTCGATACCCCCGGCCACGAGGACTTCGCCGATGACACCTATCGCACGCTGACGGCGGTCGACGC
GGCTGTCATGGTGATCGACGCGGCAAAGGGTATCGAGCCGCGCACCCTCAAGCTTTTCGAGGTCTGCCGCCTGCGCGACA
TCCCGATTATCACCTTCGTCAACAAGATGGACCGGGAGAGCCGCGATCCCTTCGAGATCCTCGACGAGGTCGAGCAGAAG
CTGGCGCTTGACTGTGCCCCGGTCACCTGGCCGATCGGCCGCTCCAAGACCTTCTGTGGCACTTATCATCTTGCCACCGA
CGAGGTGCGGGGCGCCGACACCCAGGAGCGCCTGACCAAGGTCAACGGTCCGGAAATGGTTTCGCACCGCCTGCCCGAGA
ACGAACGCGACACCTTCATCGAGGAAACGTCGCTGGCGATCGAGGCCTGCAAGCCCTTCGACCGCAAAGCCTTCCTCGAG
GGCCATCTGACGCCCGTCTTCTTCGGTTCCGCCCTTCGGAATTTCGGCGTCCGCGATCTGATCAATGCGCTTGCCGAATT
CGCGCCGCCGCCGCGGGCACAGGTTGCCGATACCCGCACCGTCGAGGCGACGGACGACAAGATGACCGCCTTCGTCTTCA
AGATCCAGGCGAACATGGACCCGAATCATCGCGACCGCATCGCCTTCGTGCGCGTCTGCTCGGGCAAGCTCGAGCGCGGC
ATGAAGGCACGGCTTTCGCGTACCGGCAAGCAGATGGGCCTGACGGCGCCGCAATTCTTCTTCGCCTCCCAGCGCCAGCT
CGCCGACACGGCGTTCGCCGGCGACGTCGTCGGTATTCCGAACCACGGCACGCTCAGGATCGGCGATACGCTGACCGAGG
GCGAACCGCTCGTCTTTCAGGGCGTTCCGAACTTCGCGCCGGAAATCCTCCGCCGCGTCCGTCTGGAAGACGCGATGAAG
GCGAAGAAGCTAAAGGAGGCGCTGCAGCAGATGGCGGAAGAGGGCGTCGTGCAGCTCTTTTCCCCGGATGACGGGGCGCC
GGCGATCGTCGGCGTCGTTGGCGCGCTGCAGCTCGACGTCCTGAAGGAACGGCTGCAGGCGGAATACAGCCTGCCGGTCT
CGTTCGAAATGTCCCGCTTCTCGATCTGCCGCTGGATCTCGGCCGAAAGCGCCGCCGATCTCGAAAAGTTCATTGCGGCC
CATCGCGGCGACATCGCGCGCGATCTCGACGGCGACCCGGTCTATATGGCGCAGGACGGATTCTCGCTGCGTTACGAATC
GGAACGCTATCCGGCGATCAGGATGGTGGCGATCAAGGAGTATCACGTCGCCAAGGCGGCGTGA

Upstream 100 bases:

>100_bases
ATGCTCATTCCTTCATAAGCACGGCCCGGCTTGCCGCAGTCAATTGCATATTGGCCCCCGAGCCTGTAAAGGACGCCGCA
ACTCACAGGATACTCAGATC

Downstream 100 bases:

>100_bases
CAGGTTCCGGGCATCCTTTCAAGGCTTGCCTGGAAGCGCTCGCGCTCCGGGAAGTGCCAGGGTTAAAACGGCGATAAGTA
CGCAGCCGATGCCGAGCACC

Product: peptide chain release factor 3

Products: NA

Alternate protein names: RF-3 [H]

Number of amino acids: Translated: 527; Mature: 526

Protein sequence:

>527_residues
MAESIAEAVSRRRTFAIISHPDAGKTTLTEKLLLFGGAIQLAGEVKAKKDRIQTRSDWMKIERERGISVVTSVMTFEYDD
TVFNLLDTPGHEDFADDTYRTLTAVDAAVMVIDAAKGIEPRTLKLFEVCRLRDIPIITFVNKMDRESRDPFEILDEVEQK
LALDCAPVTWPIGRSKTFCGTYHLATDEVRGADTQERLTKVNGPEMVSHRLPENERDTFIEETSLAIEACKPFDRKAFLE
GHLTPVFFGSALRNFGVRDLINALAEFAPPPRAQVADTRTVEATDDKMTAFVFKIQANMDPNHRDRIAFVRVCSGKLERG
MKARLSRTGKQMGLTAPQFFFASQRQLADTAFAGDVVGIPNHGTLRIGDTLTEGEPLVFQGVPNFAPEILRRVRLEDAMK
AKKLKEALQQMAEEGVVQLFSPDDGAPAIVGVVGALQLDVLKERLQAEYSLPVSFEMSRFSICRWISAESAADLEKFIAA
HRGDIARDLDGDPVYMAQDGFSLRYESERYPAIRMVAIKEYHVAKAA

Sequences:

>Translated_527_residues
MAESIAEAVSRRRTFAIISHPDAGKTTLTEKLLLFGGAIQLAGEVKAKKDRIQTRSDWMKIERERGISVVTSVMTFEYDD
TVFNLLDTPGHEDFADDTYRTLTAVDAAVMVIDAAKGIEPRTLKLFEVCRLRDIPIITFVNKMDRESRDPFEILDEVEQK
LALDCAPVTWPIGRSKTFCGTYHLATDEVRGADTQERLTKVNGPEMVSHRLPENERDTFIEETSLAIEACKPFDRKAFLE
GHLTPVFFGSALRNFGVRDLINALAEFAPPPRAQVADTRTVEATDDKMTAFVFKIQANMDPNHRDRIAFVRVCSGKLERG
MKARLSRTGKQMGLTAPQFFFASQRQLADTAFAGDVVGIPNHGTLRIGDTLTEGEPLVFQGVPNFAPEILRRVRLEDAMK
AKKLKEALQQMAEEGVVQLFSPDDGAPAIVGVVGALQLDVLKERLQAEYSLPVSFEMSRFSICRWISAESAADLEKFIAA
HRGDIARDLDGDPVYMAQDGFSLRYESERYPAIRMVAIKEYHVAKAA
>Mature_526_residues
AESIAEAVSRRRTFAIISHPDAGKTTLTEKLLLFGGAIQLAGEVKAKKDRIQTRSDWMKIERERGISVVTSVMTFEYDDT
VFNLLDTPGHEDFADDTYRTLTAVDAAVMVIDAAKGIEPRTLKLFEVCRLRDIPIITFVNKMDRESRDPFEILDEVEQKL
ALDCAPVTWPIGRSKTFCGTYHLATDEVRGADTQERLTKVNGPEMVSHRLPENERDTFIEETSLAIEACKPFDRKAFLEG
HLTPVFFGSALRNFGVRDLINALAEFAPPPRAQVADTRTVEATDDKMTAFVFKIQANMDPNHRDRIAFVRVCSGKLERGM
KARLSRTGKQMGLTAPQFFFASQRQLADTAFAGDVVGIPNHGTLRIGDTLTEGEPLVFQGVPNFAPEILRRVRLEDAMKA
KKLKEALQQMAEEGVVQLFSPDDGAPAIVGVVGALQLDVLKERLQAEYSLPVSFEMSRFSICRWISAESAADLEKFIAAH
RGDIARDLDGDPVYMAQDGFSLRYESERYPAIRMVAIKEYHVAKAA

Specific function: Increases the formation of ribosomal termination complexes and stimulates activities of RF-1 and RF-2. It binds guanine nucleotides and has strong preference for UGA stop codons. It may interact directly with the ribosome. The stimulation of RF- 1 and RF-

COG id: COG4108

COG function: function code J; Peptide chain release factor RF-3

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. PrfC subfamily [H]

Homologues:

Organism=Homo sapiens, GI18390331, Length=477, Percent_Identity=26.8343815513627, Blast_Score=168, Evalue=1e-41,
Organism=Homo sapiens, GI19923640, Length=504, Percent_Identity=26.3888888888889, Blast_Score=143, Evalue=4e-34,
Organism=Homo sapiens, GI25306283, Length=397, Percent_Identity=28.2115869017632, Blast_Score=133, Evalue=4e-31,
Organism=Homo sapiens, GI25306287, Length=294, Percent_Identity=31.2925170068027, Blast_Score=124, Evalue=2e-28,
Organism=Homo sapiens, GI157426893, Length=172, Percent_Identity=30.8139534883721, Blast_Score=96, Evalue=7e-20,
Organism=Homo sapiens, GI94966754, Length=139, Percent_Identity=30.9352517985612, Blast_Score=78, Evalue=2e-14,
Organism=Escherichia coli, GI1790835, Length=526, Percent_Identity=51.3307984790874, Blast_Score=543, Evalue=1e-155,
Organism=Escherichia coli, GI1789738, Length=488, Percent_Identity=26.0245901639344, Blast_Score=144, Evalue=2e-35,
Organism=Escherichia coli, GI1788922, Length=157, Percent_Identity=33.1210191082803, Blast_Score=96, Evalue=4e-21,
Organism=Escherichia coli, GI48994988, Length=149, Percent_Identity=32.2147651006711, Blast_Score=88, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17533571, Length=485, Percent_Identity=26.1855670103093, Blast_Score=169, Evalue=3e-42,
Organism=Caenorhabditis elegans, GI17556745, Length=473, Percent_Identity=24.1014799154334, Blast_Score=126, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI17557151, Length=132, Percent_Identity=33.3333333333333, Blast_Score=86, Evalue=4e-17,
Organism=Saccharomyces cerevisiae, GI6323098, Length=476, Percent_Identity=26.2605042016807, Blast_Score=169, Evalue=1e-42,
Organism=Saccharomyces cerevisiae, GI6322359, Length=531, Percent_Identity=25.2354048964218, Blast_Score=136, Evalue=1e-32,
Organism=Saccharomyces cerevisiae, GI6323320, Length=157, Percent_Identity=28.6624203821656, Blast_Score=81, Evalue=4e-16,
Organism=Saccharomyces cerevisiae, GI6324707, Length=158, Percent_Identity=27.8481012658228, Blast_Score=69, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6320593, Length=158, Percent_Identity=27.8481012658228, Blast_Score=69, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24582462, Length=483, Percent_Identity=25.4658385093168, Blast_Score=164, Evalue=1e-40,
Organism=Drosophila melanogaster, GI221458488, Length=538, Percent_Identity=26.3940520446097, Blast_Score=145, Evalue=7e-35,
Organism=Drosophila melanogaster, GI78706572, Length=160, Percent_Identity=31.875, Blast_Score=99, Evalue=6e-21,
Organism=Drosophila melanogaster, GI24585711, Length=153, Percent_Identity=31.3725490196078, Blast_Score=67, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24585713, Length=153, Percent_Identity=31.3725490196078, Blast_Score=67, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24585709, Length=153, Percent_Identity=31.3725490196078, Blast_Score=67, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009022
- InterPro:   IPR004548
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR004161
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2 [H]

EC number: NA

Molecular weight: Translated: 58814; Mature: 58683

Theoretical pI: Translated: 5.46; Mature: 5.46

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAESIAEAVSRRRTFAIISHPDAGKTTLTEKLLLFGGAIQLAGEVKAKKDRIQTRSDWMK
CCHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHCCHHEEHHHHHHHHHHHHHHHHHHH
IERERGISVVTSVMTFEYDDTVFNLLDTPGHEDFADDTYRTLTAVDAAVMVIDAAKGIEP
HHHHCCHHHHHHHHHCCCCCHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHEEECCCCCCC
RTLKLFEVCRLRDIPIITFVNKMDRESRDPFEILDEVEQKLALDCAPVTWPIGRSKTFCG
HHHHHHHHHHHCCCCCEEHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCEECCCCCCCEEE
TYHLATDEVRGADTQERLTKVNGPEMVSHRLPENERDTFIEETSLAIEACKPFDRKAFLE
EEEEECCCCCCCCHHHHHHHCCCHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHH
GHLTPVFFGSALRNFGVRDLINALAEFAPPPRAQVADTRTVEATDDKMTAFVFKIQANMD
CCCCHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCEEECCCCCEEEEEEEEEECCC
PNHRDRIAFVRVCSGKLERGMKARLSRTGKQMGLTAPQFFFASQRQLADTAFAGDVVGIP
CCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHCCCCCHHHHHHHHHHHHHHHHCCCEEECC
NHGTLRIGDTLTEGEPLVFQGVPNFAPEILRRVRLEDAMKAKKLKEALQQMAEEGVVQLF
CCCEEEECCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEE
SPDDGAPAIVGVVGALQLDVLKERLQAEYSLPVSFEMSRFSICRWISAESAADLEKFIAA
CCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEECHHHHHHHHHHCCCCHHHHHHHHHH
HRGDIARDLDGDPVYMAQDGFSLRYESERYPAIRMVAIKEYHVAKAA
HCCCCHHCCCCCEEEEECCCCEEEECCCCCCEEEEEEEHHHHHHCCC
>Mature Secondary Structure 
AESIAEAVSRRRTFAIISHPDAGKTTLTEKLLLFGGAIQLAGEVKAKKDRIQTRSDWMK
CHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHCCHHEEHHHHHHHHHHHHHHHHHHH
IERERGISVVTSVMTFEYDDTVFNLLDTPGHEDFADDTYRTLTAVDAAVMVIDAAKGIEP
HHHHCCHHHHHHHHHCCCCCHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHEEECCCCCCC
RTLKLFEVCRLRDIPIITFVNKMDRESRDPFEILDEVEQKLALDCAPVTWPIGRSKTFCG
HHHHHHHHHHHCCCCCEEHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCEECCCCCCCEEE
TYHLATDEVRGADTQERLTKVNGPEMVSHRLPENERDTFIEETSLAIEACKPFDRKAFLE
EEEEECCCCCCCCHHHHHHHCCCHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHH
GHLTPVFFGSALRNFGVRDLINALAEFAPPPRAQVADTRTVEATDDKMTAFVFKIQANMD
CCCCHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCEEECCCCCEEEEEEEEEECCC
PNHRDRIAFVRVCSGKLERGMKARLSRTGKQMGLTAPQFFFASQRQLADTAFAGDVVGIP
CCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHCCCCCHHHHHHHHHHHHHHHHCCCEEECC
NHGTLRIGDTLTEGEPLVFQGVPNFAPEILRRVRLEDAMKAKKLKEALQQMAEEGVVQLF
CCCEEEECCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEE
SPDDGAPAIVGVVGALQLDVLKERLQAEYSLPVSFEMSRFSICRWISAESAADLEKFIAA
CCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEECHHHHHHHHHHCCCCHHHHHHHHHH
HRGDIARDLDGDPVYMAQDGFSLRYESERYPAIRMVAIKEYHVAKAA
HCCCCHHCCCCCEEEEECCCCEEEECCCCCCEEEEEEEHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA