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The map label for this gene is yafJ [C]

Identifier: 150398748

GI number: 150398748

Start: 3772354

End: 3773178

Strand: Direct

Name: yafJ [C]

Synonym: Smed_3565

Alternate gene names: 150398748

Gene position: 3772354-3773178 (Clockwise)

Preceding gene: 150398738

Following gene: 150398750

Centisome position: 99.75

GC content: 64.0

Gene sequence:

>825_bases
ATGTGCCGTTGGGCAGCCTATCGCGGAGAGCCGCTCTATCTCGAAGAACTCGTGACGTCGCCCAAGCATTCCCTGATCGA
ACAGTCCCATTGCGCCGTCCGGGCTAAGACGGCGACCAATGGCGATGGCTTTGGGATTGCCTGGTACGGCGACCGGCCTG
AGCCGGGTCGCTATCGCGATATCTTGCCGGCCTGGTCGGATTGCAATCTGAAGAGCATCGCGAGGCAGATTCGATCCCCG
CTGTTCCTCGCCCATGTTCGCGCGGCGACCGGCGGCGGTACACGCCGCGACAATTGCCATCCCTTCGTCTTCGGCCGCTG
GTCCTTCATGCACAATGGTCAGGTCGGAGATTTCGAGTATTTGCGGCGGCCGATGGAGACCATGCTCGGCAACGAGCTCT
ATCAGGCGCGCACCGGCACGACGGATTCGGAACTGCTTTTCCTGCTCGCCTTGCAGTTCGGCCTCGAGCGCGATCCCCTC
GGGGCCGTCGCCGAGGCGCTCGCCTTCGTCGAGCGGCTTGCCGAACGCCTGGAGCGACCGGCACTCGTGCGCTTCACGGC
GGCTTTTTCCGACGGCCGCGACCTTTACGCGGTTCGATATGCCACCGACTGGAAGGCGCCTACACTCTATGCCGGGCCGA
TGGGGCCCGCCGGTGGATATTGCCTCGTCTCCGAGCCGCTCAACGATGATGACGATGCCTGGCTGGAGATTCCGGACGGC
AGCGCGATCGTGGTCGGCGAAAACGGAGTCGACGTTCGTCTCTTCAGGACCGGCGACGTTCCGCCGCGGCCGGTCCTGGT
CATTCCGGAAGAGGTTGAGGCGTAA

Upstream 100 bases:

>100_bases
AATGTGTTAACGCAATTTTACCATGTTCCGTTGAAACATCGCTGCTGACGGGGGCGCACGAATCATTTTCATATGGCGGG
GCCAAACCAGGGTGATGAGC

Downstream 100 bases:

>100_bases
CCGATCGTTGGTGTCGCGCTACTGGGCGGAGAGCTTCTTCTCGATCAGAGCCGCCAGCTTCTCCGCCACGTCATCCTTGC
CCATTTCTGGCCAGTCCTCC

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 274; Mature: 274

Protein sequence:

>274_residues
MCRWAAYRGEPLYLEELVTSPKHSLIEQSHCAVRAKTATNGDGFGIAWYGDRPEPGRYRDILPAWSDCNLKSIARQIRSP
LFLAHVRAATGGGTRRDNCHPFVFGRWSFMHNGQVGDFEYLRRPMETMLGNELYQARTGTTDSELLFLLALQFGLERDPL
GAVAEALAFVERLAERLERPALVRFTAAFSDGRDLYAVRYATDWKAPTLYAGPMGPAGGYCLVSEPLNDDDDAWLEIPDG
SAIVVGENGVDVRLFRTGDVPPRPVLVIPEEVEA

Sequences:

>Translated_274_residues
MCRWAAYRGEPLYLEELVTSPKHSLIEQSHCAVRAKTATNGDGFGIAWYGDRPEPGRYRDILPAWSDCNLKSIARQIRSP
LFLAHVRAATGGGTRRDNCHPFVFGRWSFMHNGQVGDFEYLRRPMETMLGNELYQARTGTTDSELLFLLALQFGLERDPL
GAVAEALAFVERLAERLERPALVRFTAAFSDGRDLYAVRYATDWKAPTLYAGPMGPAGGYCLVSEPLNDDDDAWLEIPDG
SAIVVGENGVDVRLFRTGDVPPRPVLVIPEEVEA
>Mature_274_residues
MCRWAAYRGEPLYLEELVTSPKHSLIEQSHCAVRAKTATNGDGFGIAWYGDRPEPGRYRDILPAWSDCNLKSIARQIRSP
LFLAHVRAATGGGTRRDNCHPFVFGRWSFMHNGQVGDFEYLRRPMETMLGNELYQARTGTTDSELLFLLALQFGLERDPL
GAVAEALAFVERLAERLERPALVRFTAAFSDGRDLYAVRYATDWKAPTLYAGPMGPAGGYCLVSEPLNDDDDAWLEIPDG
SAIVVGENGVDVRLFRTGDVPPRPVLVIPEEVEA

Specific function: Unknown

COG id: COG0121

COG function: function code R; Predicted glutamine amidotransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Saccharomyces cerevisiae, GI6324138, Length=301, Percent_Identity=29.9003322259136, Blast_Score=119, Evalue=4e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017808
- InterPro:   IPR017932 [H]

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 30478; Mature: 30478

Theoretical pI: Translated: 4.93; Mature: 4.93

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCRWAAYRGEPLYLEELVTSPKHSLIEQSHCAVRAKTATNGDGFGIAWYGDRPEPGRYRD
CCCCCCCCCCCEEHHHHHCCCHHHHHHHHCCEEEEEECCCCCCEEEEEECCCCCCCCHHH
ILPAWSDCNLKSIARQIRSPLFLAHVRAATGGGTRRDNCHPFVFGRWSFMHNGQVGDFEY
CCCCCCCCCHHHHHHHHHCCEEEEEEEHHCCCCCCCCCCCCEEEEEEEEEECCCCCCHHH
LRRPMETMLGNELYQARTGTTDSELLFLLALQFGLERDPLGAVAEALAFVERLAERLERP
HHHHHHHHHCCHHHHHCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCC
ALVRFTAAFSDGRDLYAVRYATDWKAPTLYAGPMGPAGGYCLVSEPLNDDDDAWLEIPDG
EEEEEEEECCCCCEEEEEEEECCCCCCEEEECCCCCCCCEEEEECCCCCCCCCEEECCCC
SAIVVGENGVDVRLFRTGDVPPRPVLVIPEEVEA
CEEEEECCCCEEEEEECCCCCCCCEEEECHHCCC
>Mature Secondary Structure
MCRWAAYRGEPLYLEELVTSPKHSLIEQSHCAVRAKTATNGDGFGIAWYGDRPEPGRYRD
CCCCCCCCCCCEEHHHHHCCCHHHHHHHHCCEEEEEECCCCCCEEEEEECCCCCCCCHHH
ILPAWSDCNLKSIARQIRSPLFLAHVRAATGGGTRRDNCHPFVFGRWSFMHNGQVGDFEY
CCCCCCCCCHHHHHHHHHCCEEEEEEEHHCCCCCCCCCCCCEEEEEEEEEECCCCCCHHH
LRRPMETMLGNELYQARTGTTDSELLFLLALQFGLERDPLGAVAEALAFVERLAERLERP
HHHHHHHHHCCHHHHHCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCC
ALVRFTAAFSDGRDLYAVRYATDWKAPTLYAGPMGPAGGYCLVSEPLNDDDDAWLEIPDG
EEEEEEEECCCCCEEEEEEEECCCCCCEEEECCCCCCCCEEEEECCCCCCCCCEEECCCC
SAIVVGENGVDVRLFRTGDVPPRPVLVIPEEVEA
CEEEEECCCCEEEEEECCCCCCCCEEEECHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]