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The map label for this gene is sspA [H]

Identifier: 150398691

GI number: 150398691

Start: 3710966

End: 3711655

Strand: Reverse

Name: sspA [H]

Synonym: Smed_3505

Alternate gene names: 150398691

Gene position: 3711655-3710966 (Counterclockwise)

Preceding gene: 150398693

Following gene: 150398690

Centisome position: 98.14

GC content: 62.46

Gene sequence:

>690_bases
ATGATGACCGCGCAGCTCACCCTGATCAGCCACCATCTTTGCCCCTATGTGCAACGCGCCGCGATCGCGCTCCGCGAAAA
AGACGTGCCCTTCGAACGCGTCGATATCGATCTCGCCAAGAAGCCCGACTGGTTCCTGAAGATCTCGCCGCTTGGGAAGG
TGCCGCTTCTGCGTATTCCACGGGATGGCGGCGAAGCGATCCTCTTCGAGAGTACCGTCATTTGCGAATATCTGGAGGAG
ACGCAGGCCGGGCCGAAGCTGCACCCCTCCGATCCGCTGGCGCGGGCGCGCCATCGCGGCTGGATGGAGTTCGGCTCCGC
AATCCTCTCCGACCTCTGGGTTTATGAGACCACGCGGGACGGCGGCACGCTTGAAGAAAAGCGCAGAATGCTCGAGATGA
AATTTGCCACCGTCGAGGCGGAACTCGACAGCGGCCCCTATTTTTCCGGCGGCGCCTTCAGCCTCGTGGACGCAGTCTTC
GCGCCGATCTTCCGCTATTTCGACGTGTTCGACAGGATTTCCGATCACCGCATCTTCGACGGCCTGCCGCGCGTCACCGC
CTGGCGCGAGGTGCTGGCCGAAAGGCCGAGCGTGAAAGCAGCGGTCGCCGAGGATTACCCGCAAAGGCTGACAGCCTTCC
TCGAAAAGCACGAGGCGGCGTTGCTCAAGACCGAAAGGGCAGCGGCCTGA

Upstream 100 bases:

>100_bases
TGTCCGCCCTTTTCGCGTTCATATATGCAATTACATCTATATTGACGCTTTTGGCAAGCCGCGTTATATGCAGATGCATC
TAAATAGAACCAGGAGACGA

Downstream 100 bases:

>100_bases
AGGCGCTTCTGTTTTCGGCGGAACCGGTCTAAAACCGCATAATATCCGCAAGCAGACGACCAGGAGCCGCCATGAACGCC
CCCCTCCAATCCGTGAAAAA

Product: glutathione S-transferase domain-containing protein

Products: HX; R-S-glutathione

Alternate protein names: NA

Number of amino acids: Translated: 229; Mature: 229

Protein sequence:

>229_residues
MMTAQLTLISHHLCPYVQRAAIALREKDVPFERVDIDLAKKPDWFLKISPLGKVPLLRIPRDGGEAILFESTVICEYLEE
TQAGPKLHPSDPLARARHRGWMEFGSAILSDLWVYETTRDGGTLEEKRRMLEMKFATVEAELDSGPYFSGGAFSLVDAVF
APIFRYFDVFDRISDHRIFDGLPRVTAWREVLAERPSVKAAVAEDYPQRLTAFLEKHEAALLKTERAAA

Sequences:

>Translated_229_residues
MMTAQLTLISHHLCPYVQRAAIALREKDVPFERVDIDLAKKPDWFLKISPLGKVPLLRIPRDGGEAILFESTVICEYLEE
TQAGPKLHPSDPLARARHRGWMEFGSAILSDLWVYETTRDGGTLEEKRRMLEMKFATVEAELDSGPYFSGGAFSLVDAVF
APIFRYFDVFDRISDHRIFDGLPRVTAWREVLAERPSVKAAVAEDYPQRLTAFLEKHEAALLKTERAAA
>Mature_229_residues
MMTAQLTLISHHLCPYVQRAAIALREKDVPFERVDIDLAKKPDWFLKISPLGKVPLLRIPRDGGEAILFESTVICEYLEE
TQAGPKLHPSDPLARARHRGWMEFGSAILSDLWVYETTRDGGTLEEKRRMLEMKFATVEAELDSGPYFSGGAFSLVDAVF
APIFRYFDVFDRISDHRIFDGLPRVTAWREVLAERPSVKAAVAEDYPQRLTAFLEKHEAALLKTERAAA

Specific function: Forms an equimolar complex with the RNA polymerase holoenzyme (RNAP) but not with the core enzyme [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GST N-terminal domain [H]

Homologues:

Organism=Homo sapiens, GI4758484, Length=224, Percent_Identity=26.7857142857143, Blast_Score=97, Evalue=1e-20,
Organism=Homo sapiens, GI300360534, Length=215, Percent_Identity=27.4418604651163, Blast_Score=96, Evalue=2e-20,
Organism=Homo sapiens, GI300360532, Length=219, Percent_Identity=25.1141552511416, Blast_Score=91, Evalue=9e-19,
Organism=Homo sapiens, GI38016131, Length=204, Percent_Identity=26.9607843137255, Blast_Score=90, Evalue=2e-18,
Organism=Homo sapiens, GI300360569, Length=197, Percent_Identity=27.4111675126904, Blast_Score=89, Evalue=3e-18,
Organism=Homo sapiens, GI300360567, Length=199, Percent_Identity=25.6281407035176, Blast_Score=83, Evalue=2e-16,
Organism=Homo sapiens, GI300360571, Length=192, Percent_Identity=26.0416666666667, Blast_Score=83, Evalue=2e-16,
Organism=Escherichia coli, GI87081789, Length=211, Percent_Identity=27.9620853080569, Blast_Score=67, Evalue=7e-13,
Organism=Escherichia coli, GI1789624, Length=162, Percent_Identity=31.4814814814815, Blast_Score=62, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI193207701, Length=203, Percent_Identity=28.5714285714286, Blast_Score=84, Evalue=6e-17,
Organism=Caenorhabditis elegans, GI25144449, Length=203, Percent_Identity=26.1083743842365, Blast_Score=79, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI115532656, Length=209, Percent_Identity=30.622009569378, Blast_Score=77, Evalue=9e-15,
Organism=Caenorhabditis elegans, GI115532652, Length=209, Percent_Identity=30.622009569378, Blast_Score=77, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI115532654, Length=205, Percent_Identity=27.3170731707317, Blast_Score=69, Evalue=1e-12,
Organism=Drosophila melanogaster, GI21355779, Length=220, Percent_Identity=32.7272727272727, Blast_Score=102, Evalue=3e-22,
Organism=Drosophila melanogaster, GI21355763, Length=202, Percent_Identity=33.1683168316832, Blast_Score=100, Evalue=1e-21,
Organism=Drosophila melanogaster, GI21355775, Length=204, Percent_Identity=31.8627450980392, Blast_Score=94, Evalue=1e-19,
Organism=Drosophila melanogaster, GI21355765, Length=198, Percent_Identity=24.7474747474747, Blast_Score=82, Evalue=4e-16,
Organism=Drosophila melanogaster, GI24660979, Length=193, Percent_Identity=26.4248704663212, Blast_Score=81, Evalue=6e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010987
- InterPro:   IPR004045
- InterPro:   IPR017933
- InterPro:   IPR004046
- InterPro:   IPR012336
- InterPro:   IPR012335 [H]

Pfam domain/function: PF00043 GST_C; PF02798 GST_N [H]

EC number: 2.5.1.18

Molecular weight: Translated: 25963; Mature: 25963

Theoretical pI: Translated: 5.98; Mature: 5.98

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMTAQLTLISHHLCPYVQRAAIALREKDVPFERVDIDLAKKPDWFLKISPLGKVPLLRIP
CCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCEECCCCCCEEEEECCCCCCCEEEEC
RDGGEAILFESTVICEYLEETQAGPKLHPSDPLARARHRGWMEFGSAILSDLWVYETTRD
CCCCCEEEEHHHHHHHHHHHHCCCCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHEEECCC
GGTLEEKRRMLEMKFATVEAELDSGPYFSGGAFSLVDAVFAPIFRYFDVFDRISDHRIFD
CCCHHHHHHHHHHHHHHHEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHC
GLPRVTAWREVLAERPSVKAAVAEDYPQRLTAFLEKHEAALLKTERAAA
CCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MMTAQLTLISHHLCPYVQRAAIALREKDVPFERVDIDLAKKPDWFLKISPLGKVPLLRIP
CCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCEECCCCCCEEEEECCCCCCCEEEEC
RDGGEAILFESTVICEYLEETQAGPKLHPSDPLARARHRGWMEFGSAILSDLWVYETTRD
CCCCCEEEEHHHHHHHHHHHHCCCCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHEEECCC
GGTLEEKRRMLEMKFATVEAELDSGPYFSGGAFSLVDAVFAPIFRYFDVFDRISDHRIFD
CCCHHHHHHHHHHHHHHHEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHC
GLPRVTAWREVLAERPSVKAAVAEDYPQRLTAFLEKHEAALLKTERAAA
CCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: RX; glutathione

Specific reaction: RX + glutathione = HX + R-S-glutathione

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]