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The map label for this gene is ilvD [H]

Identifier: 150398509

GI number: 150398509

Start: 3516894

End: 3518618

Strand: Reverse

Name: ilvD [H]

Synonym: Smed_3320

Alternate gene names: 150398509

Gene position: 3518618-3516894 (Counterclockwise)

Preceding gene: 150398512

Following gene: 150398508

Centisome position: 93.04

GC content: 63.65

Gene sequence:

>1725_bases
ATGAGCAGACGCAAGAATCCGGAGCAATTTCGCAGCTTTCGCTGGTTCGGCGTCAAGGACCTTCGCTCCTTCGGCCATCG
GTCCCGACTCTATCAGATGGGCTATGACCATGCGGAACTCGCAAACAAGCCCGTCATTGCCATCATCAATACCTGGAGCG
ACATCAATCCATGCCACGCGCATCTGAGAGCCCGAGCCGAGGAAGTCAAGCGCGGCGTGTGGCAGGCGGGCGGCTTCCCG
ATCGAGCTGCCGGCCATGTCGCTGGCCGAGACCTATGTGAAGCCCACGACAATGCTCTACCGCAACTTCCTTGCAATGGA
GGTGGAAGAGCTCATCCGTTCGCATCCCGTCGACGGTGTGGTGCTGCTTGCCGGCTGCGACAAGACCACGCCGGGAACGA
TCATGGGAGCGATCCAGGTCGACCTGCCGACGATCTTCGTGCCTGCAGGGCCGATGCTGCGCGGCAATTATCGCGGCCGG
ATGCTGGGCTCCGGATCGGACGTCTGGAAATACTGGGCGGAAAAGGAAGCCGGCCGCGTCACCGACAATGAATGGAGCGA
AATGGAGCGCGGTATCGCCCGCTCCTTCGGCACCTGCATGACCATGGGCACCGCCTCGACCATGACGGCGCTCGCCGACA
CGCTGGGGCTCTCGCTGCCCGGCGCCTCGGCTATTCCCGCCGCCGATGCCGGCCACGCGCGCATGGCCGCGCTTTCCGGC
GCGCGCATCGTCGAAATGGTCTTCGAGGACCTGAAGCCGTCCGGCATCCTAACAGCGAAAGCATTCGAAAACGCGCTCGC
CGTGCACATGGCAATGGCCGGCTCGACCAATGCGATGATCCACCTGATCGCGATCGCGCGACGCTGCGGTGTTGCGCTGA
CGCTCGACGATTTCGACCGCGTTTCGGCCGAGGTCCCGGTCCTCTGCAACATCCGGCCGACCGGCGAGTACCTGATGGAG
GATTTCTACTATGCCGGCGGTCTGATCGCGCTTTGGAAACAATTGAAGCCGCTCCTGCATACCGCGGAACGCAATGTGAT
CGGCACGATCGGCGGTGCTCTCGACGAGGCCGAAGTCCATCTGCCCGAGGTGATCCGTCCACTCGACAGGCCGGTTGCCG
CGCGCGGCGGCACGGCTATCCTCAAGGGAAATCTTGCGCCGCAAGGCTGCGTCATGAAACCCGCGGCGGCCGACCCTCAC
CTACTGGAACACCGCGGGCCGGCACTCGTCTTCGACAATTACGAAACGATGATGAAAGCGGTGAACGACGAGGATCTCGA
CGTCACCGCCGACACGGTCATGATTCTGCGCAATGCGGGCCCGGTCGGCGGACCGGGCATGCCGGAATGGGGCATGCTGC
CGATCCCGAAAAAACTGCTGAAAGAGGGCGTACGCGACATGGTGCGCATTTCCGACGCCCGCATGAGCGGCACCAGCTAC
GGTGCCTGTGTCCTTCACGTTGCGCCCGAAGCCTATGTCGGCGGACCGCTGGCGGCGGTGCGCAACGGCGATATCATCGC
CCTCGACGTCGCCCGCCGCACGATCACCCTCGAAGTCGACGCGGCGGAGATCGAGCGGCGGCTCGCCGAGTGGCGCCCGC
CGGAGCGGGATTACTCGCGCGGCTACCTGAAAATGCATGCGGAGAATATCCAGCAGGCGCCGGAAGGCTGCGACTTCACC
TTCCTGCAATCGCCGCATAAACTGCCCGAGCCGGAGATTCATTGA

Upstream 100 bases:

>100_bases
AGTTTCAATGTTACGTCGTTCAAGGGTCTGCCTTCACCTGTCATTTTTCTGTACAGGCGCAATCTAGCCGACCCGCCTGG
AAATGCAAAGGAGGCCGCCA

Downstream 100 bases:

>100_bases
ACGGATACGGACCGGGCAAGCCGGCAGGAGACGTGCCTCTTCGCGGACAGGTCGAAACGAATGATGACGCGACTGCTTAT
AACCGGAGCCGCAGGCAGCC

Product: dihydroxy-acid dehydratase

Products: NA

Alternate protein names: DAD [H]

Number of amino acids: Translated: 574; Mature: 573

Protein sequence:

>574_residues
MSRRKNPEQFRSFRWFGVKDLRSFGHRSRLYQMGYDHAELANKPVIAIINTWSDINPCHAHLRARAEEVKRGVWQAGGFP
IELPAMSLAETYVKPTTMLYRNFLAMEVEELIRSHPVDGVVLLAGCDKTTPGTIMGAIQVDLPTIFVPAGPMLRGNYRGR
MLGSGSDVWKYWAEKEAGRVTDNEWSEMERGIARSFGTCMTMGTASTMTALADTLGLSLPGASAIPAADAGHARMAALSG
ARIVEMVFEDLKPSGILTAKAFENALAVHMAMAGSTNAMIHLIAIARRCGVALTLDDFDRVSAEVPVLCNIRPTGEYLME
DFYYAGGLIALWKQLKPLLHTAERNVIGTIGGALDEAEVHLPEVIRPLDRPVAARGGTAILKGNLAPQGCVMKPAAADPH
LLEHRGPALVFDNYETMMKAVNDEDLDVTADTVMILRNAGPVGGPGMPEWGMLPIPKKLLKEGVRDMVRISDARMSGTSY
GACVLHVAPEAYVGGPLAAVRNGDIIALDVARRTITLEVDAAEIERRLAEWRPPERDYSRGYLKMHAENIQQAPEGCDFT
FLQSPHKLPEPEIH

Sequences:

>Translated_574_residues
MSRRKNPEQFRSFRWFGVKDLRSFGHRSRLYQMGYDHAELANKPVIAIINTWSDINPCHAHLRARAEEVKRGVWQAGGFP
IELPAMSLAETYVKPTTMLYRNFLAMEVEELIRSHPVDGVVLLAGCDKTTPGTIMGAIQVDLPTIFVPAGPMLRGNYRGR
MLGSGSDVWKYWAEKEAGRVTDNEWSEMERGIARSFGTCMTMGTASTMTALADTLGLSLPGASAIPAADAGHARMAALSG
ARIVEMVFEDLKPSGILTAKAFENALAVHMAMAGSTNAMIHLIAIARRCGVALTLDDFDRVSAEVPVLCNIRPTGEYLME
DFYYAGGLIALWKQLKPLLHTAERNVIGTIGGALDEAEVHLPEVIRPLDRPVAARGGTAILKGNLAPQGCVMKPAAADPH
LLEHRGPALVFDNYETMMKAVNDEDLDVTADTVMILRNAGPVGGPGMPEWGMLPIPKKLLKEGVRDMVRISDARMSGTSY
GACVLHVAPEAYVGGPLAAVRNGDIIALDVARRTITLEVDAAEIERRLAEWRPPERDYSRGYLKMHAENIQQAPEGCDFT
FLQSPHKLPEPEIH
>Mature_573_residues
SRRKNPEQFRSFRWFGVKDLRSFGHRSRLYQMGYDHAELANKPVIAIINTWSDINPCHAHLRARAEEVKRGVWQAGGFPI
ELPAMSLAETYVKPTTMLYRNFLAMEVEELIRSHPVDGVVLLAGCDKTTPGTIMGAIQVDLPTIFVPAGPMLRGNYRGRM
LGSGSDVWKYWAEKEAGRVTDNEWSEMERGIARSFGTCMTMGTASTMTALADTLGLSLPGASAIPAADAGHARMAALSGA
RIVEMVFEDLKPSGILTAKAFENALAVHMAMAGSTNAMIHLIAIARRCGVALTLDDFDRVSAEVPVLCNIRPTGEYLMED
FYYAGGLIALWKQLKPLLHTAERNVIGTIGGALDEAEVHLPEVIRPLDRPVAARGGTAILKGNLAPQGCVMKPAAADPHL
LEHRGPALVFDNYETMMKAVNDEDLDVTADTVMILRNAGPVGGPGMPEWGMLPIPKKLLKEGVRDMVRISDARMSGTSYG
ACVLHVAPEAYVGGPLAAVRNGDIIALDVARRTITLEVDAAEIERRLAEWRPPERDYSRGYLKMHAENIQQAPEGCDFTF
LQSPHKLPEPEIH

Specific function: Valine and isoleucine biosynthesis; fourth step. [C]

COG id: COG0129

COG function: function code EG; Dihydroxyacid dehydratase/phosphogluconate dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ilvD/edd family [H]

Homologues:

Organism=Escherichia coli, GI48994964, Length=578, Percent_Identity=31.4878892733564, Blast_Score=270, Evalue=2e-73,
Organism=Escherichia coli, GI1788157, Length=557, Percent_Identity=30.3411131059246, Blast_Score=182, Evalue=4e-47,
Organism=Escherichia coli, GI2367371, Length=464, Percent_Identity=30.6034482758621, Blast_Score=173, Evalue=3e-44,
Organism=Escherichia coli, GI1786464, Length=544, Percent_Identity=30.5147058823529, Blast_Score=172, Evalue=6e-44,
Organism=Saccharomyces cerevisiae, GI6322476, Length=547, Percent_Identity=30.164533820841, Blast_Score=251, Evalue=3e-67,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015928
- InterPro:   IPR004404
- InterPro:   IPR000581
- InterPro:   IPR020558 [H]

Pfam domain/function: PF00920 ILVD_EDD [H]

EC number: =4.2.1.9 [H]

Molecular weight: Translated: 62783; Mature: 62652

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS00886 ILVD_EDD_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
4.7 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRRKNPEQFRSFRWFGVKDLRSFGHRSRLYQMGYDHAELANKPVIAIINTWSDINPCHA
CCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCHHHHCCCCEEEEEECCCCCCHHHH
HLRARAEEVKRGVWQAGGFPIELPAMSLAETYVKPTTMLYRNFLAMEVEELIRSHPVDGV
HHHHHHHHHHHHHHHCCCCEEECCHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHCCCCCE
VLLAGCDKTTPGTIMGAIQVDLPTIFVPAGPMLRGNYRGRMLGSGSDVWKYWAEKEAGRV
EEEECCCCCCCCCEEEEEEEECCEEEECCCCEEECCCCCCEECCCCHHHHHHHHHCCCCC
TDNEWSEMERGIARSFGTCMTMGTASTMTALADTLGLSLPGASAIPAADAGHARMAALSG
CCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHCC
ARIVEMVFEDLKPSGILTAKAFENALAVHMAMAGSTNAMIHLIAIARRCGVALTLDDFDR
HHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCEEEEECCHHH
VSAEVPVLCNIRPTGEYLMEDFYYAGGLIALWKQLKPLLHTAERNVIGTIGGALDEAEVH
CCCCCCEEEECCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCEEHHCCCCCCHHCC
LPEVIRPLDRPVAARGGTAILKGNLAPQGCVMKPAAADPHLLEHRGPALVFDNYETMMKA
HHHHHHHHCCCHHCCCCCEEEECCCCCCCCEECCCCCCCHHHHCCCCEEEECCHHHHHHH
VNDEDLDVTADTVMILRNAGPVGGPGMPEWGMLPIPKKLLKEGVRDMVRISDARMSGTSY
CCCCCCCEEHHEEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCC
GACVLHVAPEAYVGGPLAAVRNGDIIALDVARRTITLEVDAAEIERRLAEWRPPERDYSR
CEEEEEECCCHHCCCCCCEECCCCEEEEEECCEEEEEEECHHHHHHHHHHCCCCCCCCCC
GYLKMHAENIQQAPEGCDFTFLQSPHKLPEPEIH
CCEEEEHHHHHCCCCCCCEEEECCCCCCCCCCCC
>Mature Secondary Structure 
SRRKNPEQFRSFRWFGVKDLRSFGHRSRLYQMGYDHAELANKPVIAIINTWSDINPCHA
CCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCHHHHCCCCEEEEEECCCCCCHHHH
HLRARAEEVKRGVWQAGGFPIELPAMSLAETYVKPTTMLYRNFLAMEVEELIRSHPVDGV
HHHHHHHHHHHHHHHCCCCEEECCHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHCCCCCE
VLLAGCDKTTPGTIMGAIQVDLPTIFVPAGPMLRGNYRGRMLGSGSDVWKYWAEKEAGRV
EEEECCCCCCCCCEEEEEEEECCEEEECCCCEEECCCCCCEECCCCHHHHHHHHHCCCCC
TDNEWSEMERGIARSFGTCMTMGTASTMTALADTLGLSLPGASAIPAADAGHARMAALSG
CCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHCC
ARIVEMVFEDLKPSGILTAKAFENALAVHMAMAGSTNAMIHLIAIARRCGVALTLDDFDR
HHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCEEEEECCHHH
VSAEVPVLCNIRPTGEYLMEDFYYAGGLIALWKQLKPLLHTAERNVIGTIGGALDEAEVH
CCCCCCEEEECCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCEEHHCCCCCCHHCC
LPEVIRPLDRPVAARGGTAILKGNLAPQGCVMKPAAADPHLLEHRGPALVFDNYETMMKA
HHHHHHHHCCCHHCCCCCEEEECCCCCCCCEECCCCCCCHHHHCCCCEEEECCHHHHHHH
VNDEDLDVTADTVMILRNAGPVGGPGMPEWGMLPIPKKLLKEGVRDMVRISDARMSGTSY
CCCCCCCEEHHEEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCC
GACVLHVAPEAYVGGPLAAVRNGDIIALDVARRTITLEVDAAEIERRLAEWRPPERDYSR
CEEEEEECCCHHCCCCCCEECCCCEEEEEECCEEEEEEECHHHHHHHHHHCCCCCCCCCC
GYLKMHAENIQQAPEGCDFTFLQSPHKLPEPEIH
CCEEEEHHHHHCCCCCCCEEEECCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA