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The map label for this gene is ytbQ [H]

Identifier: 150398508

GI number: 150398508

Start: 3516048

End: 3516833

Strand: Reverse

Name: ytbQ [H]

Synonym: Smed_3319

Alternate gene names: 150398508

Gene position: 3516833-3516048 (Counterclockwise)

Preceding gene: 150398509

Following gene: 150398506

Centisome position: 92.99

GC content: 62.98

Gene sequence:

>786_bases
ATGATGACGCGACTGCTTATAACCGGAGCCGCAGGCAGCCTCGGCGGCTATGCGCGCGAGGGATTGAAGGACTGCGCACA
GATTTTGCGCCTTTCCGACGTGGCCGAGCTTTCTCCTGCCGGAAAGGGCGAAGAGGTGGTCCGTTGCGATCTTGCCGAGC
GCGAGGCCGTCGACGAACTCGTGCGCGATTGCGATGCGATCCTGCATCTTGGCGCTATTTCGGCAGAAGCCGAGTTCGAC
GCTCTTCTCCAGGCCAATATTCTCGGCACCTACAACCTCTACGAGGCGGCGCGCAGAGCCGGCGTAAGCCGGATCCTTTT
CGCCAGCACCAACCATGTGACCGGTTTTCACCCTGTCGGCGAAAGGCTCGACCACCTGTCGCCGCGGCGTCCCGACAGCC
TTTACGGCGTCAGCAAGTGCTTCGGGGAGGATCTCTCCCGCCTCTATTTCGACAAATACGGACTCGAGACCGCGTGTCTG
AGGATCGGCAGCTGCTTCGCCGAGCCCACGAACCGGCGCATGCTCTCCACCTGGCTGAGCCCCGGTGACTTCCGGACGCT
GATACGCCAGCTTCTCGCCGCTTCGGAAATCGGTCATCTGGTGCTCTACGGCGTCTCCGCGAACCGCGACGTCTGGTGGT
CGAACGCACATGCGGACTTTCTCGGCTGGCGCCCGCAGGACAGCAGCGAAGCCTACCGGCCAGCGATCGAAGCACGCGAA
GAGGAACCCGCGAAAGGCGTTCGCTATCAGGGCGGCCGGCATGCGGCCCTGAAGCTTTCGGATTGA

Upstream 100 bases:

>100_bases
GCAATCGCCGCATAAACTGCCCGAGCCGGAGATTCATTGAACGGATACGGACCGGGCAAGCCGGCAGGAGACGTGCCTCT
TCGCGGACAGGTCGAAACGA

Downstream 100 bases:

>100_bases
GGCTCTCTGACGGCGCCGCCGCTTGCGGGCCGAAGGTGCCTGCAGGCGGAGGCTCTTCTGCTGCCGCAGATCGAAAGGGC
GCCCGTTTCCACCCTCGGCA

Product: NAD-dependent epimerase/dehydratase

Products: dTDP-4-dehydro-6-deoxy-D-glucose; H2O [C]

Alternate protein names: NA

Number of amino acids: Translated: 261; Mature: 261

Protein sequence:

>261_residues
MMTRLLITGAAGSLGGYAREGLKDCAQILRLSDVAELSPAGKGEEVVRCDLAEREAVDELVRDCDAILHLGAISAEAEFD
ALLQANILGTYNLYEAARRAGVSRILFASTNHVTGFHPVGERLDHLSPRRPDSLYGVSKCFGEDLSRLYFDKYGLETACL
RIGSCFAEPTNRRMLSTWLSPGDFRTLIRQLLAASEIGHLVLYGVSANRDVWWSNAHADFLGWRPQDSSEAYRPAIEARE
EEPAKGVRYQGGRHAALKLSD

Sequences:

>Translated_261_residues
MMTRLLITGAAGSLGGYAREGLKDCAQILRLSDVAELSPAGKGEEVVRCDLAEREAVDELVRDCDAILHLGAISAEAEFD
ALLQANILGTYNLYEAARRAGVSRILFASTNHVTGFHPVGERLDHLSPRRPDSLYGVSKCFGEDLSRLYFDKYGLETACL
RIGSCFAEPTNRRMLSTWLSPGDFRTLIRQLLAASEIGHLVLYGVSANRDVWWSNAHADFLGWRPQDSSEAYRPAIEARE
EEPAKGVRYQGGRHAALKLSD
>Mature_261_residues
MMTRLLITGAAGSLGGYAREGLKDCAQILRLSDVAELSPAGKGEEVVRCDLAEREAVDELVRDCDAILHLGAISAEAEFD
ALLQANILGTYNLYEAARRAGVSRILFASTNHVTGFHPVGERLDHLSPRRPDSLYGVSKCFGEDLSRLYFDKYGLETACL
RIGSCFAEPTNRRMLSTWLSPGDFRTLIRQLLAASEIGHLVLYGVSANRDVWWSNAHADFLGWRPQDSSEAYRPAIEARE
EEPAKGVRYQGGRHAALKLSD

Specific function: INVOLVED IN THE SYNTHESIS OF ENTEROBACTERIAL COMMON ANTIGEN (ECA) AND REQUIRED FOR SYNTHESIS OF LIPOPOLYSACCHARIDE O-SIDE CHAINS. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: 4.2.1.46 [C]

Molecular weight: Translated: 28757; Mature: 28757

Theoretical pI: Translated: 5.91; Mature: 5.91

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMTRLLITGAAGSLGGYAREGLKDCAQILRLSDVAELSPAGKGEEVVRCDLAEREAVDEL
CCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECHHHHHHHHHH
VRDCDAILHLGAISAEAEFDALLQANILGTYNLYEAARRAGVSRILFASTNHVTGFHPVG
HHHHHHHHHHHCCCCCHHHHHHHHHHHCCCHHHHHHHHHCCCCEEEEECCCCCCCCCHHH
ERLDHLSPRRPDSLYGVSKCFGEDLSRLYFDKYGLETACLRIGSCFAEPTNRRMLSTWLS
HHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHCCCCCCHHHHHHCC
PGDFRTLIRQLLAASEIGHLVLYGVSANRDVWWSNAHADFLGWRPQDSSEAYRPAIEARE
CCHHHHHHHHHHHHHHCCEEEEEECCCCCCEEECCCCCCCCCCCCCCCCHHHHHHHHHCC
EEPAKGVRYQGGRHAALKLSD
CCCCCCCCCCCCCEEEEEECC
>Mature Secondary Structure
MMTRLLITGAAGSLGGYAREGLKDCAQILRLSDVAELSPAGKGEEVVRCDLAEREAVDEL
CCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECHHHHHHHHHH
VRDCDAILHLGAISAEAEFDALLQANILGTYNLYEAARRAGVSRILFASTNHVTGFHPVG
HHHHHHHHHHHCCCCCHHHHHHHHHHHCCCHHHHHHHHHCCCCEEEEECCCCCCCCCHHH
ERLDHLSPRRPDSLYGVSKCFGEDLSRLYFDKYGLETACLRIGSCFAEPTNRRMLSTWLS
HHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHCCCCCCHHHHHHCC
PGDFRTLIRQLLAASEIGHLVLYGVSANRDVWWSNAHADFLGWRPQDSSEAYRPAIEARE
CCHHHHHHHHHHHHHHCCEEEEEECCCCCCEEECCCCCCCCCCCCCCCCHHHHHHHHHCC
EEPAKGVRYQGGRHAALKLSD
CCCCCCCCCCCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NAD. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.093 {dTDPglucose}} [C]

Substrates: dTDPglucose [C]

Specific reaction: dTDPglucose --> dTDP-4-dehydro-6-deoxy-D-glucose + H2O [C]

General reaction: Elimination (of H2O C-O bond cleavage [C]

Inhibitor: p-Chloromercuribenzoate; TMP [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8763940; 9387221; 9384377 [H]