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The map label for this gene is hyuB [H]

Identifier: 150398496

GI number: 150398496

Start: 3496870

End: 3498555

Strand: Reverse

Name: hyuB [H]

Synonym: Smed_3306

Alternate gene names: 150398496

Gene position: 3498555-3496870 (Counterclockwise)

Preceding gene: 150398497

Following gene: 150398495

Centisome position: 92.51

GC content: 62.51

Gene sequence:

>1686_bases
ATGTTCGGCCGCATCACCGATGCCAAGGCCGACCCGATCACCACCGAGGTCATTCGCCACGCGCTGAATTCGGCCGCCAA
CCAGATGAAGCGCGCTTTGATCCGCACCTCATTTTCGCCGATCATCTACGAAGTGCTCGATTTCGCCGCGGCCATCTATG
ACAAGGAAGTCCGGCTTCTGTCGCAGGCGCCGTCGCTGCCGATGTTCATGGGGACCCTGTCCTTCTGCATCATCGAGGCC
GTCAAGGCCAATGGCGGGCCGGAAACCCTCAAGCCCGGCGATGCTCTGATCTATAACTGGCCATTCGGAACCGGCTCACA
TCCGCAGGACGTGGTCATCATCATGCCGGTCTTCTTTCGGGATGAGGAGCTGATCGGCTATACAGCGATGAAGGGACATT
GGCTGGATATCGGCGCCAAGGATCCCTATTGCACCGACACGACGGATGTTTTCCAGGAGGGCGTGATCTTCCCGGGCGTC
AAGATCTACAAGGAAAATGTTCTCAACGACGACATTTTCCGGATGATCATGGCCAATACGCGGGTTCCCCAAATGGTGCG
CGGAGACCTCGACGCGCAGGTCACGGGCTGCCGGGTGGGCGTCAAATCGCTGATCGAGGTGATCGAGCGGTTTGGGCTCG
AAACCTACCGGGCCGCGGTCGACGACATGTTCGACCATGGCGAACGTATCGTCCGCTCTTATTTCGAGAAGATCCCCGAC
GGCCGCTATGTCGGCAAGGGCGAAATGGACAATAACGGCGTCACCACGGACCGTATTCCCTTCGAGGTCGTGCTTGAAGT
CAGGGGTTCGGACGTCATCCTCGACTTTTCCGGCGTGCCCGACGAGCAGGCCGGGCCGACAAACTCGCCGCTGCCCACCA
CCATTTCGGCCAGCCGGGTGGCGATCACCATGCTGGCCGGCTATGGCGAAGCGCCGCATGAGGGTCACTTTCGGCCGCTC
AAGGTCATCACCCGCCCCGGCTCGATGTTCGACCCGCTGCCGCCGGCACCGTCCTTCATCTATGGATGGCCGGGCCTGCA
GGCGATCGAAGTCATCTATAACGCCGTCGCGCACGCCATGCCCAAGGCCGTGCCCGCCCAATCCGGCGGTTGCATCTGCT
CTGTCGTGGCCTGGGGAAGCCGGGAGGAAAGCGGCGAGCCCTGGGCGGACGGCACGCCGCTGCCGACGGGACAGGGGGCC
TGGGACGGCGGTGATGGAGGCATCATGCTGCATATCGCCGAATCCGCCACCCGCTTCACCCCGGCCGAAGTCTGGGAGCA
CCGCAATCCCTGGATCATCGAGCAGCTGGCGCTGGCCGAGGATTCCGTCGGACCGGGCAAGTGGCGCGGCGGACCTGGCC
TCAATCTCGATATCCGCATGACAGAGGACACGCTGATCACCACCGTCTTCGACCGCACGCTCAATGCCCCCTGGGGCCTG
TTGGGCGGCAAGGAGGCACGGCCGAACAATTGTTTCGCCAAAATGCCGGATGGCGAAGTTCATTCCATCGCCAAATCGAC
GCATTTCCACATGCCCAAGGATTCGGTGCTGCAAACCCGCACCGGCGGCGGCGGCGGCTATGGCGACCCCGCCGAGCGGC
CGGTCGAGATGGTCAAGCGCGACCTGGCCGATGGCTACATCACCGAGGATTATGCGCGAAAGCACTATCCGCACGCGTTT
TCGTGA

Upstream 100 bases:

>100_bases
GTCCAGTGGACCATCTCGAGCGGCAAAGCCGGCGAAATCATTTTGGAACGGACGCACTGAAATGGCTAGAAAACATGAAT
CCGTCACCCATAATGCCGGC

Downstream 100 bases:

>100_bases
CGTGATCCAGGCAGGGCTGTCGGTCGGAGGCCGGCGAAACGGGGGCGGGGACCCGACCCCCGCCCCGGATCTGAGAGACC
GCCATCATGGGAACGCGCGG

Product: 5-oxoprolinase

Products: ADP; phosphate; N-carbamoylsarcosine

Alternate protein names: ORF3 [H]

Number of amino acids: Translated: 561; Mature: 561

Protein sequence:

>561_residues
MFGRITDAKADPITTEVIRHALNSAANQMKRALIRTSFSPIIYEVLDFAAAIYDKEVRLLSQAPSLPMFMGTLSFCIIEA
VKANGGPETLKPGDALIYNWPFGTGSHPQDVVIIMPVFFRDEELIGYTAMKGHWLDIGAKDPYCTDTTDVFQEGVIFPGV
KIYKENVLNDDIFRMIMANTRVPQMVRGDLDAQVTGCRVGVKSLIEVIERFGLETYRAAVDDMFDHGERIVRSYFEKIPD
GRYVGKGEMDNNGVTTDRIPFEVVLEVRGSDVILDFSGVPDEQAGPTNSPLPTTISASRVAITMLAGYGEAPHEGHFRPL
KVITRPGSMFDPLPPAPSFIYGWPGLQAIEVIYNAVAHAMPKAVPAQSGGCICSVVAWGSREESGEPWADGTPLPTGQGA
WDGGDGGIMLHIAESATRFTPAEVWEHRNPWIIEQLALAEDSVGPGKWRGGPGLNLDIRMTEDTLITTVFDRTLNAPWGL
LGGKEARPNNCFAKMPDGEVHSIAKSTHFHMPKDSVLQTRTGGGGGYGDPAERPVEMVKRDLADGYITEDYARKHYPHAF
S

Sequences:

>Translated_561_residues
MFGRITDAKADPITTEVIRHALNSAANQMKRALIRTSFSPIIYEVLDFAAAIYDKEVRLLSQAPSLPMFMGTLSFCIIEA
VKANGGPETLKPGDALIYNWPFGTGSHPQDVVIIMPVFFRDEELIGYTAMKGHWLDIGAKDPYCTDTTDVFQEGVIFPGV
KIYKENVLNDDIFRMIMANTRVPQMVRGDLDAQVTGCRVGVKSLIEVIERFGLETYRAAVDDMFDHGERIVRSYFEKIPD
GRYVGKGEMDNNGVTTDRIPFEVVLEVRGSDVILDFSGVPDEQAGPTNSPLPTTISASRVAITMLAGYGEAPHEGHFRPL
KVITRPGSMFDPLPPAPSFIYGWPGLQAIEVIYNAVAHAMPKAVPAQSGGCICSVVAWGSREESGEPWADGTPLPTGQGA
WDGGDGGIMLHIAESATRFTPAEVWEHRNPWIIEQLALAEDSVGPGKWRGGPGLNLDIRMTEDTLITTVFDRTLNAPWGL
LGGKEARPNNCFAKMPDGEVHSIAKSTHFHMPKDSVLQTRTGGGGGYGDPAERPVEMVKRDLADGYITEDYARKHYPHAF
S
>Mature_561_residues
MFGRITDAKADPITTEVIRHALNSAANQMKRALIRTSFSPIIYEVLDFAAAIYDKEVRLLSQAPSLPMFMGTLSFCIIEA
VKANGGPETLKPGDALIYNWPFGTGSHPQDVVIIMPVFFRDEELIGYTAMKGHWLDIGAKDPYCTDTTDVFQEGVIFPGV
KIYKENVLNDDIFRMIMANTRVPQMVRGDLDAQVTGCRVGVKSLIEVIERFGLETYRAAVDDMFDHGERIVRSYFEKIPD
GRYVGKGEMDNNGVTTDRIPFEVVLEVRGSDVILDFSGVPDEQAGPTNSPLPTTISASRVAITMLAGYGEAPHEGHFRPL
KVITRPGSMFDPLPPAPSFIYGWPGLQAIEVIYNAVAHAMPKAVPAQSGGCICSVVAWGSREESGEPWADGTPLPTGQGA
WDGGDGGIMLHIAESATRFTPAEVWEHRNPWIIEQLALAEDSVGPGKWRGGPGLNLDIRMTEDTLITTVFDRTLNAPWGL
LGGKEARPNNCFAKMPDGEVHSIAKSTHFHMPKDSVLQTRTGGGGGYGDPAERPVEMVKRDLADGYITEDYARKHYPHAF
S

Specific function: HyuA and hyuB convert D- and L-substituted hydantoins to corresponding N-carbamyl-amino acids [H]

COG id: COG0146

COG function: function code EQ; N-methylhydantoinase B/acetone carboxylase, alpha subunit

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the oxoprolinase family [H]

Homologues:

Organism=Homo sapiens, GI48314820, Length=545, Percent_Identity=29.3577981651376, Blast_Score=167, Evalue=2e-41,
Organism=Caenorhabditis elegans, GI133901900, Length=544, Percent_Identity=27.0220588235294, Blast_Score=175, Evalue=5e-44,
Organism=Caenorhabditis elegans, GI133901902, Length=509, Percent_Identity=26.3261296660118, Blast_Score=155, Evalue=5e-38,
Organism=Saccharomyces cerevisiae, GI6322634, Length=552, Percent_Identity=25.3623188405797, Blast_Score=167, Evalue=5e-42,
Organism=Drosophila melanogaster, GI45550492, Length=548, Percent_Identity=27.3722627737226, Blast_Score=182, Evalue=5e-46,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003692 [H]

Pfam domain/function: PF02538 Hydantoinase_B [H]

EC number: 3.5.2.14

Molecular weight: Translated: 61444; Mature: 61444

Theoretical pI: Translated: 4.96; Mature: 4.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFGRITDAKADPITTEVIRHALNSAANQMKRALIRTSFSPIIYEVLDFAAAIYDKEVRLL
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHH
SQAPSLPMFMGTLSFCIIEAVKANGGPETLKPGDALIYNWPFGTGSHPQDVVIIMPVFFR
HCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCCCCCCCCEEEEEEEEEC
DEELIGYTAMKGHWLDIGAKDPYCTDTTDVFQEGVIFPGVKIYKENVLNDDIFRMIMANT
CCHHEEEEECCCEEEEECCCCCCCCCHHHHHHCCCCCCCHHHHHHHCCCHHHHHHHHHCC
RVPQMVRGDLDAQVTGCRVGVKSLIEVIERFGLETYRAAVDDMFDHGERIVRSYFEKIPD
CCCHHHHCCCCCEEHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHCCC
GRYVGKGEMDNNGVTTDRIPFEVVLEVRGSDVILDFSGVPDEQAGPTNSPLPTTISASRV
CCEECCCCCCCCCCCCCCCCEEEEEEECCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCE
AITMLAGYGEAPHEGHFRPLKVITRPGSMFDPLPPAPSFIYGWPGLQAIEVIYNAVAHAM
EEEEEECCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCEEECCCCHHHHHHHHHHHHHHH
PKAVPAQSGGCICSVVAWGSREESGEPWADGTPLPTGQGAWDGGDGGIMLHIAESATRFT
CCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCC
PAEVWEHRNPWIIEQLALAEDSVGPGKWRGGPGLNLDIRMTEDTLITTVFDRTLNAPWGL
HHHHHHCCCCHHHHHHHHHCCCCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHCCCCCCC
LGGKEARPNNCFAKMPDGEVHSIAKSTHFHMPKDSVLQTRTGGGGGYGDPAERPVEMVKR
CCCCCCCCCCCEEECCCCHHHHHHHHHCCCCCCHHHHEECCCCCCCCCCHHHHHHHHHHH
DLADGYITEDYARKHYPHAFS
HHCCCCCCHHHHHHCCCCCCC
>Mature Secondary Structure
MFGRITDAKADPITTEVIRHALNSAANQMKRALIRTSFSPIIYEVLDFAAAIYDKEVRLL
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHH
SQAPSLPMFMGTLSFCIIEAVKANGGPETLKPGDALIYNWPFGTGSHPQDVVIIMPVFFR
HCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCCCCCCCCEEEEEEEEEC
DEELIGYTAMKGHWLDIGAKDPYCTDTTDVFQEGVIFPGVKIYKENVLNDDIFRMIMANT
CCHHEEEEECCCEEEEECCCCCCCCCHHHHHHCCCCCCCHHHHHHHCCCHHHHHHHHHCC
RVPQMVRGDLDAQVTGCRVGVKSLIEVIERFGLETYRAAVDDMFDHGERIVRSYFEKIPD
CCCHHHHCCCCCEEHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHCCC
GRYVGKGEMDNNGVTTDRIPFEVVLEVRGSDVILDFSGVPDEQAGPTNSPLPTTISASRV
CCEECCCCCCCCCCCCCCCCEEEEEEECCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCE
AITMLAGYGEAPHEGHFRPLKVITRPGSMFDPLPPAPSFIYGWPGLQAIEVIYNAVAHAM
EEEEEECCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCEEECCCCHHHHHHHHHHHHHHH
PKAVPAQSGGCICSVVAWGSREESGEPWADGTPLPTGQGAWDGGDGGIMLHIAESATRFT
CCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCC
PAEVWEHRNPWIIEQLALAEDSVGPGKWRGGPGLNLDIRMTEDTLITTVFDRTLNAPWGL
HHHHHHCCCCHHHHHHHHHCCCCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHCCCCCCC
LGGKEARPNNCFAKMPDGEVHSIAKSTHFHMPKDSVLQTRTGGGGGYGDPAERPVEMVKR
CCCCCCCCCCCEEECCCCHHHHHHHHHCCCCCCHHHHEECCCCCCCCCCHHHHHHHHHHH
DLADGYITEDYARKHYPHAFS
HHCCCCCCHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; N-methylimidazolidine-2,4-dione; H2O

Specific reaction: ATP + N-methylimidazolidine-2,4-dione + 2 H2O = ADP + phosphate + N-carbamoylsarcosine

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1732229 [H]