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The map label for this gene is hisF [H]

Identifier: 150398451

GI number: 150398451

Start: 3439495

End: 3440271

Strand: Reverse

Name: hisF [H]

Synonym: Smed_3259

Alternate gene names: 150398451

Gene position: 3440271-3439495 (Counterclockwise)

Preceding gene: 150398452

Following gene: 150398450

Centisome position: 90.97

GC content: 63.58

Gene sequence:

>777_bases
ATGACGCTGAAAGCCCGTGTGATCCCCTGCCTCGACGTCAAGGACGGCCGTGTCGTCAAAGGCGTCAATTTCGTCGATCT
CATCGATGCCGGCGATCCGGTGGAGGCGGCACGCGCCTATGACGCGGCCGGCGCTGACGAACTCTGTTTTCTCGACATCA
CCGCCTCCTCCGACAATCGCGAAACGATCTTCGACGTTATCGCCCGCACCGCCGAACAGTGCTTCATGCCGCTGACCGTC
GGGGGCGGCGTGCGGCAGGTCTCCGATATCCGCAAGCTCCTGCTCGCCGGCGCCGACAAGGTGTCCATCAACACGGCTGC
GGTGAAGAACCCGGACTTCGTGGCGGAAGCCGCCGACAAGTTCGGCGACCAGTGCATCGTCGTCGCCATCGACGCCAAGA
AAGTCTCGGATCCAGGTGAAACCGACCGGTGGGAGATCTTCACCCATGGCGGGCGGCAGCCTACAGGCATGGATGCAGTC
GAATTCGCACGGAAGGTCGTCGATCTCGGCGCCGGCGAGATCCTCCTGACGTCGATGGATCGCGACGGCACCAAAAGCGG
CTACGACATCAGCCTGACGAGGACGATTGCTGACGCGGTGCGTGCGCCGGTCATCGCGTCGGGGGGCGTGGGTACGCTCG
ATCATCTGGTCGAAGGTATCCGCGACGGCCACGCGACCGCCGTGCTCGCGGCATCCATTTTTCACTTCGGAACATACAGC
ATCGGTGAGGCGAAGCGCCACATGGCCGAGCACGGCGTCGCCATGCGTCTCGATTGA

Upstream 100 bases:

>100_bases
AACTCGAAGGGGCAATCTCCGGCCGGGCGCTTTATGACGGTCGAATCGATCCGAAGGAAGCGCTCGATCTGATCCGCGAA
GCCAGGAAGGGGATGATCCG

Downstream 100 bases:

>100_bases
AGCGGCCACGAGAAGGGATAGACCATGACAGAGTTCACCCTCTCCGATCTGGAGAAGATCGTGGCGACCCGCGCCAGGGC
GGCTCCGGAGGAGTCCTGGA

Product: imidazole glycerol phosphate synthase subunit HisF

Products: NA

Alternate protein names: IGP synthase cyclase subunit; IGP synthase subunit hisF; ImGP synthase subunit hisF; IGPS subunit hisF [H]

Number of amino acids: Translated: 258; Mature: 257

Protein sequence:

>258_residues
MTLKARVIPCLDVKDGRVVKGVNFVDLIDAGDPVEAARAYDAAGADELCFLDITASSDNRETIFDVIARTAEQCFMPLTV
GGGVRQVSDIRKLLLAGADKVSINTAAVKNPDFVAEAADKFGDQCIVVAIDAKKVSDPGETDRWEIFTHGGRQPTGMDAV
EFARKVVDLGAGEILLTSMDRDGTKSGYDISLTRTIADAVRAPVIASGGVGTLDHLVEGIRDGHATAVLAASIFHFGTYS
IGEAKRHMAEHGVAMRLD

Sequences:

>Translated_258_residues
MTLKARVIPCLDVKDGRVVKGVNFVDLIDAGDPVEAARAYDAAGADELCFLDITASSDNRETIFDVIARTAEQCFMPLTV
GGGVRQVSDIRKLLLAGADKVSINTAAVKNPDFVAEAADKFGDQCIVVAIDAKKVSDPGETDRWEIFTHGGRQPTGMDAV
EFARKVVDLGAGEILLTSMDRDGTKSGYDISLTRTIADAVRAPVIASGGVGTLDHLVEGIRDGHATAVLAASIFHFGTYS
IGEAKRHMAEHGVAMRLD
>Mature_257_residues
TLKARVIPCLDVKDGRVVKGVNFVDLIDAGDPVEAARAYDAAGADELCFLDITASSDNRETIFDVIARTAEQCFMPLTVG
GGVRQVSDIRKLLLAGADKVSINTAAVKNPDFVAEAADKFGDQCIVVAIDAKKVSDPGETDRWEIFTHGGRQPTGMDAVE
FARKVVDLGAGEILLTSMDRDGTKSGYDISLTRTIADAVRAPVIASGGVGTLDHLVEGIRDGHATAVLAASIFHFGTYSI
GEAKRHMAEHGVAMRLD

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisF subunit catalyzes the cyclization activity that produces IGP and AICAR from PRFAR using the ammonia provided by the hisH subunit [H]

COG id: COG0107

COG function: function code E; Imidazoleglycerol-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hisA/hisF family [H]

Homologues:

Organism=Escherichia coli, GI1788336, Length=259, Percent_Identity=41.6988416988417, Blast_Score=203, Evalue=8e-54,
Organism=Escherichia coli, GI87082028, Length=244, Percent_Identity=29.0983606557377, Blast_Score=93, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6319725, Length=315, Percent_Identity=32.6984126984127, Blast_Score=152, Evalue=6e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006062
- InterPro:   IPR004651
- InterPro:   IPR011060 [H]

Pfam domain/function: PF00977 His_biosynth [H]

EC number: 4.1.3.-

Molecular weight: Translated: 27374; Mature: 27243

Theoretical pI: Translated: 4.80; Mature: 4.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLKARVIPCLDVKDGRVVKGVNFVDLIDAGDPVEAARAYDAAGADELCFLDITASSDNR
CCCCCEEEEEEECCCCCEEECCCEEEEECCCCCHHHHHHHCCCCCCCEEEEEEECCCCCH
ETIFDVIARTAEQCFMPLTVGGGVRQVSDIRKLLLAGADKVSINTAAVKNPDFVAEAADK
HHHHHHHHHHHHHHEEEEEECCCHHHHHHHHHHHHCCCCCEEEEEEECCCCHHHHHHHHH
FGDQCIVVAIDAKKVSDPGETDRWEIFTHGGRQPTGMDAVEFARKVVDLGAGEILLTSMD
CCCEEEEEEEECEECCCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHCCCCEEEEEECC
RDGTKSGYDISLTRTIADAVRAPVIASGGVGTLDHLVEGIRDGHATAVLAASIFHFGTYS
CCCCCCCCEEEHHHHHHHHHHCCEEECCCCCHHHHHHHHHCCCCHHHHHHHHHHHHCCCC
IGEAKRHMAEHGVAMRLD
HHHHHHHHHHCCEEEECC
>Mature Secondary Structure 
TLKARVIPCLDVKDGRVVKGVNFVDLIDAGDPVEAARAYDAAGADELCFLDITASSDNR
CCCCEEEEEEECCCCCEEECCCEEEEECCCCCHHHHHHHCCCCCCCEEEEEEECCCCCH
ETIFDVIARTAEQCFMPLTVGGGVRQVSDIRKLLLAGADKVSINTAAVKNPDFVAEAADK
HHHHHHHHHHHHHHEEEEEECCCHHHHHHHHHHHHCCCCCEEEEEEECCCCHHHHHHHHH
FGDQCIVVAIDAKKVSDPGETDRWEIFTHGGRQPTGMDAVEFARKVVDLGAGEILLTSMD
CCCEEEEEEEECEECCCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHCCCCEEEEEECC
RDGTKSGYDISLTRTIADAVRAPVIASGGVGTLDHLVEGIRDGHATAVLAASIFHFGTYS
CCCCCCCCEEEHHHHHHHHHHCCEEECCCCCHHHHHHHHHCCCCHHHHHHHHHHHHCCCC
IGEAKRHMAEHGVAMRLD
HHHHHHHHHHCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Lyases; Carbon-Nitrogen Lyases; Amidine-Lyases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA