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The map label for this gene is prpE [C]

Identifier: 150398388

GI number: 150398388

Start: 3368314

End: 3370221

Strand: Reverse

Name: prpE [C]

Synonym: Smed_3196

Alternate gene names: 150398388

Gene position: 3370221-3368314 (Counterclockwise)

Preceding gene: 150398389

Following gene: 150398386

Centisome position: 89.11

GC content: 62.0

Gene sequence:

>1908_bases
ATGGCGAGCCGCTATCGCGAAGTGTACGCCGCGTGGAAAGCTGATCCGCAGGGATTCTGGGCAGATGCCGCATCGGCGAT
CGACTGGATCAAGCAGCCGGAGCGCGTTTTCGACCCGGAGGGCGGCACCTATGGCCACTGGTTTCCGGACGGCATGACCA
ACACCTGTCACAATTGCCTAGACCGGCATGTCGAGGCTGGCCGTGGCGAGCAACCGGCCCTCATATATGACAGTCCCGTC
ACCGGCGTGACCGAGAAGATCTCCTATGCGGACCTGCTAGCCGATGTGAAGGCAATGGCTGCAATCTACCACGAGCTCGG
CGTAGTCAAAGGCGATCGCATCATCATCTACATGCCGATGATCCCGCAGGCCGCTATCGCCATGCTCGCGGCAGCGCGGA
TCGGCGCGGTGCATTCCGTCGTTTTCGGCGGTTTTGCCGCCAATGAGCTGGCCGTCCGCATCGATGACTGCCAGGCGAAA
CTCGTCGTCTCCGCAAGCTGCGGGCTCGAGCCCGGGCGGACCGTGGCCTACAAGCCTCTGCTCGACCAGGCGATTGAAAC
GGCAACCCACAAGCCCGCCCATTGCCTGATCTACCAGCGCGGCATGCTCGATGCCGCCATGGTTCAAGATCGCGACATCG
ACTTCGCCGAGGCGCTTGCGGAGGCCAAGGATAAAGGCGAGGAGGCACCTTGCGCCTCCGTCGCCTCGACGGATCCGCTC
TATGTGCTCTATACTTCCGGAACGACCGGACAGCCGAAAGGCGTTGTGCGTGACAATGGCGGGCATATGGTCGCGCTCAG
ATGGTCGATGGAGCATTTTTTCGGCGTGAATGCCGGCGAGGTGTTCTGGGCCGCTTCCGATATCGGCTGGGTCGTCGGCC
ACTCCTATATCGTCTATGGTCCGCTCCTCAACGGATCCACATCGATCCTCTTCGAAGGAAAGCCGGTCGGCACACCCGAC
CCCGGAACCTATTGGCGCGTCATTTCCGAACACGGCGTCGCCGTCACGTTCACCGCGCCGACGGCACTGCGTGCGATCCG
CAAGGAGGATCCGGAAGCAAATCACGTGCAGCGATACGACCTATCGAAATTTCGGGCGCTCTACCTCGCGGGCGAGCGGG
CCGACCCGGACACGATCCGCTGGGCGGAACGGGCGCTGAAGGTCCCGGTCATCGATCATTGGTGGCAGACGGAAACAGGC
TGGCCCGTCGCCGGCAATCCGCTGGGTCTTGGCCTCCTGCCGGTGAAATACGGTTCGCCGGCGGTTCCCCTTCCGGGTTA
TGACGTACAGGTCGTGGATGACGCGGGCCATCCGGTAGGATCCGGCACGCTCGGCAATGTCGTGATCAAGCTGCCGTTGC
CGCCCGGCTGCCTGCCGACGCTCTGGAATGCCGATCGCCGCTTTCATGCAGCCTATCTCGAAGAATATCCCGGCTTCTAC
AAGACGGCCGATGCCGGCTATGTCGACGACGACGGCTACATCTTCATCATGGCGCGGACCGACGACATCATCAATGTGGC
GGGACATCGGCTGTCGACGGGCGCCATGGAAGAGGTCTGCGCCAGCCATCCGGATGTCGCGGAGTGTGCCGTGATCGGTA
TTGCTGACTCCCTCAAAGGGCAGGTTCCGGCAGGCTTCCTCGTCGTCAACGCCAATGTTTCCCGTGAAACAGCGGAAATA
GAGAAGGAGGTCATCGCTCTCGTGCGCGAGCGCATCGGGCCGGTAGCCGCCTTCAGGACCGCGGTTTGCGTCAAGCGCCT
CCCCAAGACGCGGTCCGGCAAGATCCTGCGCTCCACGATCCAGAAGATCATTGATCGCCAACCCTGGAAAATGCCGGCGA
CGATCGACGATCCAGCAATTCTGGAAGAAATAGCCGATGTGCTGCGGGCGAAGGGGATTGGTGTTTAA

Upstream 100 bases:

>100_bases
TCACCCTTTCGTCGGGGTTCCCGTTTACGTCCGTTCGCCTATCTTTCGAGGCGGAGGAGCCGGGAATTTTGTCCGGAAGA
GCGAGTGGGAGGAACGGGAT

Downstream 100 bases:

>100_bases
AAGGGGATTGATGTTTAAAGTGGATGAAGGCCAACAAAAAACCCGGCTCCTGCGATCCGGGTTCTTTAATCGTCATCGCC
GACGAATCGATCAATAGTCG

Product: AMP-dependent synthetase and ligase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 635; Mature: 634

Protein sequence:

>635_residues
MASRYREVYAAWKADPQGFWADAASAIDWIKQPERVFDPEGGTYGHWFPDGMTNTCHNCLDRHVEAGRGEQPALIYDSPV
TGVTEKISYADLLADVKAMAAIYHELGVVKGDRIIIYMPMIPQAAIAMLAAARIGAVHSVVFGGFAANELAVRIDDCQAK
LVVSASCGLEPGRTVAYKPLLDQAIETATHKPAHCLIYQRGMLDAAMVQDRDIDFAEALAEAKDKGEEAPCASVASTDPL
YVLYTSGTTGQPKGVVRDNGGHMVALRWSMEHFFGVNAGEVFWAASDIGWVVGHSYIVYGPLLNGSTSILFEGKPVGTPD
PGTYWRVISEHGVAVTFTAPTALRAIRKEDPEANHVQRYDLSKFRALYLAGERADPDTIRWAERALKVPVIDHWWQTETG
WPVAGNPLGLGLLPVKYGSPAVPLPGYDVQVVDDAGHPVGSGTLGNVVIKLPLPPGCLPTLWNADRRFHAAYLEEYPGFY
KTADAGYVDDDGYIFIMARTDDIINVAGHRLSTGAMEEVCASHPDVAECAVIGIADSLKGQVPAGFLVVNANVSRETAEI
EKEVIALVRERIGPVAAFRTAVCVKRLPKTRSGKILRSTIQKIIDRQPWKMPATIDDPAILEEIADVLRAKGIGV

Sequences:

>Translated_635_residues
MASRYREVYAAWKADPQGFWADAASAIDWIKQPERVFDPEGGTYGHWFPDGMTNTCHNCLDRHVEAGRGEQPALIYDSPV
TGVTEKISYADLLADVKAMAAIYHELGVVKGDRIIIYMPMIPQAAIAMLAAARIGAVHSVVFGGFAANELAVRIDDCQAK
LVVSASCGLEPGRTVAYKPLLDQAIETATHKPAHCLIYQRGMLDAAMVQDRDIDFAEALAEAKDKGEEAPCASVASTDPL
YVLYTSGTTGQPKGVVRDNGGHMVALRWSMEHFFGVNAGEVFWAASDIGWVVGHSYIVYGPLLNGSTSILFEGKPVGTPD
PGTYWRVISEHGVAVTFTAPTALRAIRKEDPEANHVQRYDLSKFRALYLAGERADPDTIRWAERALKVPVIDHWWQTETG
WPVAGNPLGLGLLPVKYGSPAVPLPGYDVQVVDDAGHPVGSGTLGNVVIKLPLPPGCLPTLWNADRRFHAAYLEEYPGFY
KTADAGYVDDDGYIFIMARTDDIINVAGHRLSTGAMEEVCASHPDVAECAVIGIADSLKGQVPAGFLVVNANVSRETAEI
EKEVIALVRERIGPVAAFRTAVCVKRLPKTRSGKILRSTIQKIIDRQPWKMPATIDDPAILEEIADVLRAKGIGV
>Mature_634_residues
ASRYREVYAAWKADPQGFWADAASAIDWIKQPERVFDPEGGTYGHWFPDGMTNTCHNCLDRHVEAGRGEQPALIYDSPVT
GVTEKISYADLLADVKAMAAIYHELGVVKGDRIIIYMPMIPQAAIAMLAAARIGAVHSVVFGGFAANELAVRIDDCQAKL
VVSASCGLEPGRTVAYKPLLDQAIETATHKPAHCLIYQRGMLDAAMVQDRDIDFAEALAEAKDKGEEAPCASVASTDPLY
VLYTSGTTGQPKGVVRDNGGHMVALRWSMEHFFGVNAGEVFWAASDIGWVVGHSYIVYGPLLNGSTSILFEGKPVGTPDP
GTYWRVISEHGVAVTFTAPTALRAIRKEDPEANHVQRYDLSKFRALYLAGERADPDTIRWAERALKVPVIDHWWQTETGW
PVAGNPLGLGLLPVKYGSPAVPLPGYDVQVVDDAGHPVGSGTLGNVVIKLPLPPGCLPTLWNADRRFHAAYLEEYPGFYK
TADAGYVDDDGYIFIMARTDDIINVAGHRLSTGAMEEVCASHPDVAECAVIGIADSLKGQVPAGFLVVNANVSRETAEIE
KEVIALVRERIGPVAAFRTAVCVKRLPKTRSGKILRSTIQKIIDRQPWKMPATIDDPAILEEIADVLRAKGIGV

Specific function: Catalyzes The Synthesis Of Propionyl-CoA From Propionate And CoA. Also Converts Acetate To Acetyl-CoA But With A Lower Specific Activity (By Similarity). [C]

COG id: COG0365

COG function: function code I; Acyl-coenzyme A synthetases/AMP-(fatty) acid ligases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI13375727, Length=629, Percent_Identity=49.2845786963434, Blast_Score=662, Evalue=0.0,
Organism=Homo sapiens, GI8923896, Length=660, Percent_Identity=35.1515151515151, Blast_Score=371, Evalue=1e-102,
Organism=Homo sapiens, GI253970489, Length=673, Percent_Identity=34.6210995542348, Blast_Score=365, Evalue=1e-101,
Organism=Homo sapiens, GI28416953, Length=630, Percent_Identity=36.8253968253968, Blast_Score=361, Evalue=1e-99,
Organism=Homo sapiens, GI38505220, Length=555, Percent_Identity=28.8288288288288, Blast_Score=191, Evalue=2e-48,
Organism=Homo sapiens, GI42544132, Length=593, Percent_Identity=24.7892074198988, Blast_Score=167, Evalue=3e-41,
Organism=Homo sapiens, GI115511026, Length=553, Percent_Identity=24.9547920433996, Blast_Score=157, Evalue=3e-38,
Organism=Homo sapiens, GI122937307, Length=507, Percent_Identity=24.8520710059172, Blast_Score=153, Evalue=5e-37,
Organism=Homo sapiens, GI58082049, Length=508, Percent_Identity=24.6062992125984, Blast_Score=148, Evalue=2e-35,
Organism=Homo sapiens, GI157311624, Length=511, Percent_Identity=25.0489236790607, Blast_Score=147, Evalue=3e-35,
Organism=Homo sapiens, GI157311622, Length=511, Percent_Identity=25.0489236790607, Blast_Score=147, Evalue=3e-35,
Organism=Homo sapiens, GI31982927, Length=638, Percent_Identity=23.0407523510972, Blast_Score=137, Evalue=3e-32,
Organism=Homo sapiens, GI195947378, Length=452, Percent_Identity=24.5575221238938, Blast_Score=102, Evalue=8e-22,
Organism=Homo sapiens, GI42544134, Length=453, Percent_Identity=21.4128035320088, Blast_Score=84, Evalue=3e-16,
Organism=Homo sapiens, GI187761345, Length=385, Percent_Identity=24.1558441558442, Blast_Score=70, Evalue=8e-12,
Organism=Homo sapiens, GI187761343, Length=385, Percent_Identity=24.1558441558442, Blast_Score=70, Evalue=8e-12,
Organism=Escherichia coli, GI1786529, Length=631, Percent_Identity=45.9587955625991, Blast_Score=512, Evalue=1e-146,
Organism=Escherichia coli, GI1790505, Length=638, Percent_Identity=36.5203761755486, Blast_Score=404, Evalue=1e-114,
Organism=Escherichia coli, GI221142682, Length=538, Percent_Identity=23.7918215613383, Blast_Score=127, Evalue=3e-30,
Organism=Escherichia coli, GI1788107, Length=547, Percent_Identity=23.217550274223, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI145693145, Length=518, Percent_Identity=23.1660231660232, Blast_Score=96, Evalue=8e-21,
Organism=Escherichia coli, GI1786801, Length=559, Percent_Identity=22.0035778175313, Blast_Score=88, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI71983001, Length=621, Percent_Identity=32.5281803542673, Blast_Score=312, Evalue=3e-85,
Organism=Caenorhabditis elegans, GI71982997, Length=621, Percent_Identity=32.5281803542673, Blast_Score=312, Evalue=3e-85,
Organism=Caenorhabditis elegans, GI17557194, Length=541, Percent_Identity=24.2144177449168, Blast_Score=99, Evalue=8e-21,
Organism=Caenorhabditis elegans, GI71985884, Length=384, Percent_Identity=24.7395833333333, Blast_Score=97, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17531443, Length=536, Percent_Identity=22.5746268656716, Blast_Score=97, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17559526, Length=566, Percent_Identity=21.7314487632509, Blast_Score=88, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17538037, Length=178, Percent_Identity=26.9662921348315, Blast_Score=78, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6319264, Length=646, Percent_Identity=36.2229102167183, Blast_Score=407, Evalue=1e-114,
Organism=Saccharomyces cerevisiae, GI6323182, Length=637, Percent_Identity=36.2637362637363, Blast_Score=377, Evalue=1e-105,
Organism=Saccharomyces cerevisiae, GI6319699, Length=370, Percent_Identity=24.5945945945946, Blast_Score=85, Evalue=3e-17,
Organism=Drosophila melanogaster, GI21355225, Length=634, Percent_Identity=43.5331230283912, Blast_Score=572, Evalue=1e-163,
Organism=Drosophila melanogaster, GI24667955, Length=649, Percent_Identity=35.2850539291217, Blast_Score=363, Evalue=1e-100,
Organism=Drosophila melanogaster, GI62472339, Length=558, Percent_Identity=36.9175627240143, Blast_Score=338, Evalue=8e-93,
Organism=Drosophila melanogaster, GI24667959, Length=527, Percent_Identity=36.8121442125237, Blast_Score=314, Evalue=1e-85,
Organism=Drosophila melanogaster, GI24648260, Length=363, Percent_Identity=26.7217630853994, Blast_Score=95, Evalue=1e-19,
Organism=Drosophila melanogaster, GI21356947, Length=377, Percent_Identity=25.7294429708223, Blast_Score=89, Evalue=9e-18,
Organism=Drosophila melanogaster, GI21355181, Length=527, Percent_Identity=23.719165085389, Blast_Score=89, Evalue=1e-17,
Organism=Drosophila melanogaster, GI24648253, Length=529, Percent_Identity=22.4952741020794, Blast_Score=88, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24648255, Length=529, Percent_Identity=22.4952741020794, Blast_Score=88, Evalue=2e-17,
Organism=Drosophila melanogaster, GI18859661, Length=517, Percent_Identity=24.3713733075435, Blast_Score=87, Evalue=3e-17,
Organism=Drosophila melanogaster, GI21356441, Length=375, Percent_Identity=24, Blast_Score=85, Evalue=1e-16,
Organism=Drosophila melanogaster, GI281365686, Length=383, Percent_Identity=22.4543080939948, Blast_Score=82, Evalue=1e-15,
Organism=Drosophila melanogaster, GI21358303, Length=340, Percent_Identity=22.0588235294118, Blast_Score=73, Evalue=5e-13,
Organism=Drosophila melanogaster, GI24653035, Length=371, Percent_Identity=22.911051212938, Blast_Score=72, Evalue=1e-12,
Organism=Drosophila melanogaster, GI20130357, Length=376, Percent_Identity=24.2021276595745, Blast_Score=65, Evalue=1e-10,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: 6.2.1.17 [C]

Molecular weight: Translated: 68894; Mature: 68763

Theoretical pI: Translated: 5.48; Mature: 5.48

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASRYREVYAAWKADPQGFWADAASAIDWIKQPERVFDPEGGTYGHWFPDGMTNTCHNCL
CCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCHHHCCCCCCCCCCCCCCCCHHHHHHHH
DRHVEAGRGEQPALIYDSPVTGVTEKISYADLLADVKAMAAIYHELGVVKGDRIIIYMPM
HHHHHCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCEECCEEEEEECC
IPQAAIAMLAAARIGAVHSVVFGGFAANELAVRIDDCQAKLVVSASCGLEPGRTVAYKPL
CCHHHHHHHHHHHHHHHHHHHHCCEECCCEEEEEECCCEEEEEEECCCCCCCCEEEEHHH
LDQAIETATHKPAHCLIYQRGMLDAAMVQDRDIDFAEALAEAKDKGEEAPCASVASTDPL
HHHHHHHHCCCCCEEEEEECCCCHHHHHCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCE
YVLYTSGTTGQPKGVVRDNGGHMVALRWSMEHFFGVNAGEVFWAASDIGWVVGHSYIVYG
EEEEECCCCCCCCCCEEECCCEEEEEEECHHHHHCCCCCCEEEEECCCCHHHCCCEEEEE
PLLNGSTSILFEGKPVGTPDPGTYWRVISEHGVAVTFTAPTALRAIRKEDPEANHVQRYD
ECCCCCEEEEECCCCCCCCCCCCEEEEEECCCEEEEEECHHHHHHHHHCCCCCCCHHHHH
LSKFRALYLAGERADPDTIRWAERALKVPVIDHWWQTETGWPVAGNPLGLGLLPVKYGSP
HHHHEEEEEECCCCCCHHHHHHHHHHCCCEECCCCCCCCCCCCCCCCCCCEEEEEECCCC
AVPLPGYDVQVVDDAGHPVGSGTLGNVVIKLPLPPGCLPTLWNADRRFHAAYLEEYPGFY
CCCCCCCCEEEEECCCCCCCCCCCCCEEEEECCCCCCCHHHHCCCHHHHHHHHHHCCCCE
KTADAGYVDDDGYIFIMARTDDIINVAGHRLSTGAMEEVCASHPDVAECAVIGIADSLKG
EECCCCEECCCCEEEEEECCCCHHHHCCCCCCCHHHHHHHCCCCCHHHEEEEEEHHHCCC
QVPAGFLVVNANVSRETAEIEKEVIALVRERIGPVAAFRTAVCVKRLPKTRSGKILRSTI
CCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCCCCCHHHHHHH
QKIIDRQPWKMPATIDDPAILEEIADVLRAKGIGV
HHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
ASRYREVYAAWKADPQGFWADAASAIDWIKQPERVFDPEGGTYGHWFPDGMTNTCHNCL
CCHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCHHHCCCCCCCCCCCCCCCCHHHHHHHH
DRHVEAGRGEQPALIYDSPVTGVTEKISYADLLADVKAMAAIYHELGVVKGDRIIIYMPM
HHHHHCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCEECCEEEEEECC
IPQAAIAMLAAARIGAVHSVVFGGFAANELAVRIDDCQAKLVVSASCGLEPGRTVAYKPL
CCHHHHHHHHHHHHHHHHHHHHCCEECCCEEEEEECCCEEEEEEECCCCCCCCEEEEHHH
LDQAIETATHKPAHCLIYQRGMLDAAMVQDRDIDFAEALAEAKDKGEEAPCASVASTDPL
HHHHHHHHCCCCCEEEEEECCCCHHHHHCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCE
YVLYTSGTTGQPKGVVRDNGGHMVALRWSMEHFFGVNAGEVFWAASDIGWVVGHSYIVYG
EEEEECCCCCCCCCCEEECCCEEEEEEECHHHHHCCCCCCEEEEECCCCHHHCCCEEEEE
PLLNGSTSILFEGKPVGTPDPGTYWRVISEHGVAVTFTAPTALRAIRKEDPEANHVQRYD
ECCCCCEEEEECCCCCCCCCCCCEEEEEECCCEEEEEECHHHHHHHHHCCCCCCCHHHHH
LSKFRALYLAGERADPDTIRWAERALKVPVIDHWWQTETGWPVAGNPLGLGLLPVKYGSP
HHHHEEEEEECCCCCCHHHHHHHHHHCCCEECCCCCCCCCCCCCCCCCCCEEEEEECCCC
AVPLPGYDVQVVDDAGHPVGSGTLGNVVIKLPLPPGCLPTLWNADRRFHAAYLEEYPGFY
CCCCCCCCEEEEECCCCCCCCCCCCCEEEEECCCCCCCHHHHCCCHHHHHHHHHHCCCCE
KTADAGYVDDDGYIFIMARTDDIINVAGHRLSTGAMEEVCASHPDVAECAVIGIADSLKG
EECCCCEECCCCEEEEEECCCCHHHHCCCCCCCHHHHHHHCCCCCHHHEEEEEEHHHCCC
QVPAGFLVVNANVSRETAEIEKEVIALVRERIGPVAAFRTAVCVKRLPKTRSGKILRSTI
CCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCCCCCHHHHHHH
QKIIDRQPWKMPATIDDPAILEEIADVLRAKGIGV
HHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043; 1339433 [H]