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The map label for this gene is rsmB [H]

Identifier: 150398383

GI number: 150398383

Start: 3362382

End: 3363794

Strand: Reverse

Name: rsmB [H]

Synonym: Smed_3191

Alternate gene names: 150398383

Gene position: 3363794-3362382 (Counterclockwise)

Preceding gene: 150398384

Following gene: 150398382

Centisome position: 88.94

GC content: 64.69

Gene sequence:

>1413_bases
ATGCCCGAAGACAGCAAGAACGATTCTCATCCGAAACGAATCCGAGAACACAGGCGCCCCGGATTGGGAGGGCCCAAGGC
CGGCGCGCTTGCCCCGGCCAAACCGGGCTTGAAGAGCCGCCAGGCAGCGGCGAAGATCCTTGCGGCGGTGGTCGACCGGA
AAACGCCGCTCGACGGGATGCTGGACCCGGAGCGGGGCAACCCGGTCTATCGTGAGTTGAACGATGCCGACCGCGCGCTG
GTGCGCGCCATTCTGAACTCCGCACTGCGCCATCTGCCACGCATCCGCGCGGCGATCGATTCCCTGCTGCAGACACCATT
GCCTGAGGGTGCGCGGGCACTCGAACATGTCCTGACCGTTGCTGCCGCACAAATTCTCTATCTTGATGTTCCGGATCATT
CCGCCGTCGACCTCGCAGTGGAACAGGCCCAGTCCGATCCGCGAAATCGCCGTTTCGCCAGCCTTGTCAATGCGGTGCTG
CGGCGGCTTTCGCGTGAAAAGGAGGCGATTCTCGAACGTCTGCAACAGGTGCCGGCGATGCCGGGCTGGTTCTTCGACAG
GCTCGTCACCTGTTACGGACGCGATCAAGCGGAGCGCATCTCTGACGCGCAGCTCGTACCCGCCGCCATCGATGTCACCG
TTAAATCCGATGCAGCTTCCTGGGCCGACCGGCTCAACGGCACCGTGTTGCCGACCGGTTCCGTTCGACTTCAGGATTTT
GACGGCTCCATTCCGTCTCTGCCCGGTTTTTCGGAGGGCGGATGGTGGGTCCAGGATGCGGCTGCCTCCATTCCGGCGCG
CCTCTTCGGAGATATTTCCGGCAGAAAGGTGGTCGACCTTTGTGCCGCGCCCGGCGGCAAGACGGCGCAACTCGTTCTCG
CAGGGGCAAAGGTGATCGCGCTGGATCAGTCTTCCAGCCGCCTCCGGCGACTCAAGGCCAACCTTGAGCGGCTTGGATTA
GAGAGCGCGACGAAGGAGGCCGACATGGCCGATTTCCGGCCGGAAGAACTCTTCGACGCCGCCCTTCTCGACGCCCCCTG
CTCGTCGACCGGCACCACCCGGCGGCATCCCGATGTGCTCTGGACAAAAGGCCCTGATGACGTCGAGAAGCTTGCCGGGC
TGCAAGAGCGGCTGCTGCGGCATGCACTCACCCTGGTGAAGCCCGGAGGGCTTCTCGTCTTTTCCAACTGTTCGCTCGAC
CCGCGCGAGGGCGAGGAGGTCGTGGCGCGCGTCGTTGCCGACGGCGGATGCGAGCGGGTGCCGATCGACGCGGCCGCCTG
GCCTGGGCTCGAACAGGCGATTACCGCCCTCGGAGAATTCCGGACGACACCGGCGATGCTGTCCCTTGGACCGCCTTTCG
CCAGCGGTCTGGATGGTTTCTATGCGGCCGTGTTGCGCCGGGCCGACGCCTGA

Upstream 100 bases:

>100_bases
CACCCGTTCGGCCTGATAATGCGGTGCGAAAATCGCGCTCTGTCCCGAGAACCGGCTGGGGTCGCGGTGGTTCCGAACCG
CCGAAAGGCCCCTGGTCCTG

Downstream 100 bases:

>100_bases
TGCTTGTCGACAGGTTTGATCGTGACAATTGACGCATTGAGGCGGTCCGCCTATCAATGACTTCAGGATTGATAAATATT
TAATGATCTTTCCCGCACGA

Product: Fmu (Sun) domain-containing protein

Products: NA

Alternate protein names: 16S rRNA m5C967 methyltransferase; rRNA (cytosine-C(5)-)-methyltransferase rsmB [H]

Number of amino acids: Translated: 470; Mature: 469

Protein sequence:

>470_residues
MPEDSKNDSHPKRIREHRRPGLGGPKAGALAPAKPGLKSRQAAAKILAAVVDRKTPLDGMLDPERGNPVYRELNDADRAL
VRAILNSALRHLPRIRAAIDSLLQTPLPEGARALEHVLTVAAAQILYLDVPDHSAVDLAVEQAQSDPRNRRFASLVNAVL
RRLSREKEAILERLQQVPAMPGWFFDRLVTCYGRDQAERISDAQLVPAAIDVTVKSDAASWADRLNGTVLPTGSVRLQDF
DGSIPSLPGFSEGGWWVQDAAASIPARLFGDISGRKVVDLCAAPGGKTAQLVLAGAKVIALDQSSSRLRRLKANLERLGL
ESATKEADMADFRPEELFDAALLDAPCSSTGTTRRHPDVLWTKGPDDVEKLAGLQERLLRHALTLVKPGGLLVFSNCSLD
PREGEEVVARVVADGGCERVPIDAAAWPGLEQAITALGEFRTTPAMLSLGPPFASGLDGFYAAVLRRADA

Sequences:

>Translated_470_residues
MPEDSKNDSHPKRIREHRRPGLGGPKAGALAPAKPGLKSRQAAAKILAAVVDRKTPLDGMLDPERGNPVYRELNDADRAL
VRAILNSALRHLPRIRAAIDSLLQTPLPEGARALEHVLTVAAAQILYLDVPDHSAVDLAVEQAQSDPRNRRFASLVNAVL
RRLSREKEAILERLQQVPAMPGWFFDRLVTCYGRDQAERISDAQLVPAAIDVTVKSDAASWADRLNGTVLPTGSVRLQDF
DGSIPSLPGFSEGGWWVQDAAASIPARLFGDISGRKVVDLCAAPGGKTAQLVLAGAKVIALDQSSSRLRRLKANLERLGL
ESATKEADMADFRPEELFDAALLDAPCSSTGTTRRHPDVLWTKGPDDVEKLAGLQERLLRHALTLVKPGGLLVFSNCSLD
PREGEEVVARVVADGGCERVPIDAAAWPGLEQAITALGEFRTTPAMLSLGPPFASGLDGFYAAVLRRADA
>Mature_469_residues
PEDSKNDSHPKRIREHRRPGLGGPKAGALAPAKPGLKSRQAAAKILAAVVDRKTPLDGMLDPERGNPVYRELNDADRALV
RAILNSALRHLPRIRAAIDSLLQTPLPEGARALEHVLTVAAAQILYLDVPDHSAVDLAVEQAQSDPRNRRFASLVNAVLR
RLSREKEAILERLQQVPAMPGWFFDRLVTCYGRDQAERISDAQLVPAAIDVTVKSDAASWADRLNGTVLPTGSVRLQDFD
GSIPSLPGFSEGGWWVQDAAASIPARLFGDISGRKVVDLCAAPGGKTAQLVLAGAKVIALDQSSSRLRRLKANLERLGLE
SATKEADMADFRPEELFDAALLDAPCSSTGTTRRHPDVLWTKGPDDVEKLAGLQERLLRHALTLVKPGGLLVFSNCSLDP
REGEEVVARVVADGGCERVPIDAAAWPGLEQAITALGEFRTTPAMLSLGPPFASGLDGFYAAVLRRADA

Specific function: Specifically methylates the cytosine at position 967 (m5C967) of 16S rRNA [H]

COG id: COG0144

COG function: function code J; tRNA and rRNA cytosine-C5-methylases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmB/NOP family [H]

Homologues:

Organism=Homo sapiens, GI76150625, Length=245, Percent_Identity=31.8367346938775, Blast_Score=104, Evalue=2e-22,
Organism=Homo sapiens, GI76150623, Length=245, Percent_Identity=31.8367346938775, Blast_Score=104, Evalue=2e-22,
Organism=Homo sapiens, GI32698918, Length=181, Percent_Identity=35.3591160220994, Blast_Score=91, Evalue=3e-18,
Organism=Homo sapiens, GI23199998, Length=237, Percent_Identity=33.3333333333333, Blast_Score=79, Evalue=1e-14,
Organism=Homo sapiens, GI270288818, Length=237, Percent_Identity=33.3333333333333, Blast_Score=79, Evalue=1e-14,
Organism=Homo sapiens, GI270288816, Length=238, Percent_Identity=33.6134453781513, Blast_Score=79, Evalue=1e-14,
Organism=Homo sapiens, GI8922322, Length=237, Percent_Identity=33.3333333333333, Blast_Score=79, Evalue=1e-14,
Organism=Homo sapiens, GI11545785, Length=188, Percent_Identity=31.9148936170213, Blast_Score=78, Evalue=2e-14,
Organism=Homo sapiens, GI301336155, Length=177, Percent_Identity=33.8983050847458, Blast_Score=74, Evalue=3e-13,
Organism=Homo sapiens, GI39995082, Length=177, Percent_Identity=33.8983050847458, Blast_Score=74, Evalue=3e-13,
Organism=Homo sapiens, GI40316918, Length=213, Percent_Identity=32.8638497652582, Blast_Score=68, Evalue=2e-11,
Organism=Escherichia coli, GI2367212, Length=383, Percent_Identity=32.6370757180157, Blast_Score=176, Evalue=2e-45,
Organism=Escherichia coli, GI87081985, Length=260, Percent_Identity=31.9230769230769, Blast_Score=109, Evalue=5e-25,
Organism=Caenorhabditis elegans, GI17536757, Length=243, Percent_Identity=30.0411522633745, Blast_Score=103, Evalue=3e-22,
Organism=Caenorhabditis elegans, GI71998419, Length=247, Percent_Identity=28.7449392712551, Blast_Score=80, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI115534021, Length=247, Percent_Identity=28.7449392712551, Blast_Score=79, Evalue=4e-15,
Organism=Caenorhabditis elegans, GI25143471, Length=176, Percent_Identity=33.5227272727273, Blast_Score=74, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6324268, Length=257, Percent_Identity=29.9610894941634, Blast_Score=92, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6319447, Length=185, Percent_Identity=34.5945945945946, Blast_Score=77, Evalue=8e-15,
Organism=Drosophila melanogaster, GI22024126, Length=244, Percent_Identity=29.9180327868852, Blast_Score=107, Evalue=1e-23,
Organism=Drosophila melanogaster, GI21355201, Length=193, Percent_Identity=32.6424870466321, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24668781, Length=195, Percent_Identity=32.8205128205128, Blast_Score=82, Evalue=1e-15,
Organism=Drosophila melanogaster, GI21356513, Length=195, Percent_Identity=32.8205128205128, Blast_Score=81, Evalue=2e-15,
Organism=Drosophila melanogaster, GI19920494, Length=190, Percent_Identity=27.8947368421053, Blast_Score=70, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001678
- InterPro:   IPR018314
- InterPro:   IPR006027
- InterPro:   IPR004573 [H]

Pfam domain/function: PF01189 Nol1_Nop2_Fmu; PF01029 NusB [H]

EC number: =2.1.1.176 [H]

Molecular weight: Translated: 50596; Mature: 50465

Theoretical pI: Translated: 7.17; Mature: 7.17

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPEDSKNDSHPKRIREHRRPGLGGPKAGALAPAKPGLKSRQAAAKILAAVVDRKTPLDGM
CCCCCCCCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCC
LDPERGNPVYRELNDADRALVRAILNSALRHLPRIRAAIDSLLQTPLPEGARALEHVLTV
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
AAAQILYLDVPDHSAVDLAVEQAQSDPRNRRFASLVNAVLRRLSREKEAILERLQQVPAM
HHHHHEEEECCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
PGWFFDRLVTCYGRDQAERISDAQLVPAAIDVTVKSDAASWADRLNGTVLPTGSVRLQDF
CCHHHHHHHHHHCCCHHHHHCCCCCCCEEEEEEECCHHHHHHHHCCCCEECCCCEEEEEC
DGSIPSLPGFSEGGWWVQDAAASIPARLFGDISGRKVVDLCAAPGGKTAQLVLAGAKVIA
CCCCCCCCCCCCCCCCHHHHHHHCCHHHHHCCCCCHHHHHHCCCCCCCEEEEEECCEEEE
LDQSSSRLRRLKANLERLGLESATKEADMADFRPEELFDAALLDAPCSSTGTTRRHPDVL
ECCCHHHHHHHHHHHHHHCCHHHHCCCCHHCCCHHHHHHHHHHCCCCCCCCCCCCCCCEE
WTKGPDDVEKLAGLQERLLRHALTLVKPGGLLVFSNCSLDPREGEEVVARVVADGGCERV
ECCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCHHHHHHHHHCCCCCCC
PIDAAAWPGLEQAITALGEFRTTPAMLSLGPPFASGLDGFYAAVLRRADA
CCCHHHCCCHHHHHHHHHHHCCCCHHEECCCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
PEDSKNDSHPKRIREHRRPGLGGPKAGALAPAKPGLKSRQAAAKILAAVVDRKTPLDGM
CCCCCCCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCC
LDPERGNPVYRELNDADRALVRAILNSALRHLPRIRAAIDSLLQTPLPEGARALEHVLTV
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
AAAQILYLDVPDHSAVDLAVEQAQSDPRNRRFASLVNAVLRRLSREKEAILERLQQVPAM
HHHHHEEEECCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
PGWFFDRLVTCYGRDQAERISDAQLVPAAIDVTVKSDAASWADRLNGTVLPTGSVRLQDF
CCHHHHHHHHHHCCCHHHHHCCCCCCCEEEEEEECCHHHHHHHHCCCCEECCCCEEEEEC
DGSIPSLPGFSEGGWWVQDAAASIPARLFGDISGRKVVDLCAAPGGKTAQLVLAGAKVIA
CCCCCCCCCCCCCCCCHHHHHHHCCHHHHHCCCCCHHHHHHCCCCCCCEEEEEECCEEEE
LDQSSSRLRRLKANLERLGLESATKEADMADFRPEELFDAALLDAPCSSTGTTRRHPDVL
ECCCHHHHHHHHHHHHHHCCHHHHCCCCHHCCCHHHHHHHHHHCCCCCCCCCCCCCCCEE
WTKGPDDVEKLAGLQERLLRHALTLVKPGGLLVFSNCSLDPREGEEVVARVVADGGCERV
ECCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCHHHHHHHHHCCCCCCC
PIDAAAWPGLEQAITALGEFRTTPAMLSLGPPFASGLDGFYAAVLRRADA
CCCHHHCCCHHHHHHHHHHHCCCCHHEECCCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA