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The map label for this gene is purH [H]

Identifier: 150398381

GI number: 150398381

Start: 3358668

End: 3360278

Strand: Reverse

Name: purH [H]

Synonym: Smed_3189

Alternate gene names: 150398381

Gene position: 3360278-3358668 (Counterclockwise)

Preceding gene: 150398382

Following gene: 150398378

Centisome position: 88.85

GC content: 64.06

Gene sequence:

>1611_bases
ATGGCTGTCGCCTCCAAGAAAATTCCCGCCCCGGACGAAGTCCGGATCAAAACCGCCCTCCTTTCGGTCTCCGACAAATC
GGGCATCGTCGAACTCGCCCGCCACCTCAATGACAGGGGCGTGCGGCTGGTATCGACCGGCGGGACGCACAAGGCGCTTG
CCGATGCGGGCCTTCCCGTCAGCGACGTTTCGGAGCTGACGGGGTTTCCGGAGATCATGGACGGTCGCGTGAAAACCCTT
CATCCCGGCGTTCACGGCGGTCTGCTGGCTATTCGCGACGATGCGGAGCATGCCGGCGCGATGAGCGCACACGGTATTAC
AGCGATCGATCTCGCCGTCATCAATCTCTACCCCTTCGAGGAGGTTCGCGCCAAGGGCGGCGACTATCCGACCACGGTCG
AGAATATCGACATTGGCGGTCCCGCAATGATCCGGGCGTCGGCCAAGAACCACGCCTATGTGACCGTTGTGACCGACCCG
GCGGATTATCCGCTGCTGCTGGAGGAGATCGCGGGCGGCACGACGCGCTATGCCTTCCGCCAGAAAATGGCCGCCAAGGC
CTATGCCCGCACAGCGGCCTATGATGCGGCAATCTCCAACTGGTTCGCCGAGGTACTCGACACGCCCATGCCGCGCCACC
GCGTCATCGGCGGGGTGCTCAAGGAAGAGATGCGCTACGGCGAAAACCCGCATCAGAAGGCCGGTTTCTATGTGACCGGT
GACAAGCGGCCGGGGGTCGCCACTGCAGCGCTTCTCCAAGGCAAGCAGCTCTCCTACAACAACATCAACGACACCGACGC
CGCCTTCGAGCTGGTGGCGGAATTCCTGCCGGAAAAGGCGCCTGCCTGCGCCATTATCAAGCATGCCAACCCCTGCGGCG
TTGCGACCGCGCCATCGCTCGCGGAAGCATATCGCCGGGCGCTTGCCTGTGATTCGACCTCCGCTTTCGGCGGCATTATC
GCGCTTAATCAGGAACTCGACGCGGCGACCGCCGAAGAGATCGTGAAGCTCTTCACCGAAGTGATCATCGCCCCGTCGGT
CAGCGACGAGGCGAAAGCGATCATCGCCCGGAAGCCCAATCTGCGGCTGCTTGCGACCGGCGGCCTGCCGGATCCGCGCA
CGCCCGGTCTGACGGCAAAGACGGTGGCCGGGGGCCTTCTTGTCCAGACGCGCGACGACGGCATGATCGAAGACATCGAA
CTGAAGGTGGTCACGAAGCGCACGCCGACGGCGCAGGAGCTCGAAGACATGAAATTTGCCTTCAAGGTGGCCAAGCACGT
CAAGTCGAATGCCGTCGTCTACGCGAAAGGCGGTCAGACGGCGGGTATCGGCGCCGGACAGATGAGCCGGGTCGATTCCG
CGAGAATTGCTGCCATCAAGGCGGAAGAGGCGGCGAAGGCGCTCGGTCTCGCCGAGCCTCTGACACGCGGCTCCGCGGTT
GCCTCGGAAGCCTTCCTGCCGTTCGCTGACGGCCTTCTGTCCGCGATCGCTGCGGGGGCCACCGCAGTGATCCAGCCGGG
CGGCTCCATGCGCGACGAGGAGGTGATCGCAGCGGCCGACGAGCACAATGTCGCGATGGTCTTCACCGGGATGCGGCATT
TCCGGCACTGA

Upstream 100 bases:

>100_bases
CCGAAGGTTTCTTCCCGGATGAAGCTATGCTAACGGGCAGCCAGCCCAACCGCCGGAACCTCCTCCTCACGCGTCCGGCG
CTGCCAAAGGAGCAAGGTCG

Downstream 100 bases:

>100_bases
GCGGCGCCTCTGATCCTGCTGCAGGCGAAATGCCACAACCTCGGTTGTGGCCCCCTCACGTCGGGCGATGAGCCGGATAG
GGCGTCCGCAGGAGAAGGAC

Product: bifunctional phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase

Products: NA

Alternate protein names: Phosphoribosylaminoimidazolecarboxamide formyltransferase; AICAR transformylase; IMP cyclohydrolase; ATIC; IMP synthase; Inosinicase [H]

Number of amino acids: Translated: 536; Mature: 535

Protein sequence:

>536_residues
MAVASKKIPAPDEVRIKTALLSVSDKSGIVELARHLNDRGVRLVSTGGTHKALADAGLPVSDVSELTGFPEIMDGRVKTL
HPGVHGGLLAIRDDAEHAGAMSAHGITAIDLAVINLYPFEEVRAKGGDYPTTVENIDIGGPAMIRASAKNHAYVTVVTDP
ADYPLLLEEIAGGTTRYAFRQKMAAKAYARTAAYDAAISNWFAEVLDTPMPRHRVIGGVLKEEMRYGENPHQKAGFYVTG
DKRPGVATAALLQGKQLSYNNINDTDAAFELVAEFLPEKAPACAIIKHANPCGVATAPSLAEAYRRALACDSTSAFGGII
ALNQELDAATAEEIVKLFTEVIIAPSVSDEAKAIIARKPNLRLLATGGLPDPRTPGLTAKTVAGGLLVQTRDDGMIEDIE
LKVVTKRTPTAQELEDMKFAFKVAKHVKSNAVVYAKGGQTAGIGAGQMSRVDSARIAAIKAEEAAKALGLAEPLTRGSAV
ASEAFLPFADGLLSAIAAGATAVIQPGGSMRDEEVIAAADEHNVAMVFTGMRHFRH

Sequences:

>Translated_536_residues
MAVASKKIPAPDEVRIKTALLSVSDKSGIVELARHLNDRGVRLVSTGGTHKALADAGLPVSDVSELTGFPEIMDGRVKTL
HPGVHGGLLAIRDDAEHAGAMSAHGITAIDLAVINLYPFEEVRAKGGDYPTTVENIDIGGPAMIRASAKNHAYVTVVTDP
ADYPLLLEEIAGGTTRYAFRQKMAAKAYARTAAYDAAISNWFAEVLDTPMPRHRVIGGVLKEEMRYGENPHQKAGFYVTG
DKRPGVATAALLQGKQLSYNNINDTDAAFELVAEFLPEKAPACAIIKHANPCGVATAPSLAEAYRRALACDSTSAFGGII
ALNQELDAATAEEIVKLFTEVIIAPSVSDEAKAIIARKPNLRLLATGGLPDPRTPGLTAKTVAGGLLVQTRDDGMIEDIE
LKVVTKRTPTAQELEDMKFAFKVAKHVKSNAVVYAKGGQTAGIGAGQMSRVDSARIAAIKAEEAAKALGLAEPLTRGSAV
ASEAFLPFADGLLSAIAAGATAVIQPGGSMRDEEVIAAADEHNVAMVFTGMRHFRH
>Mature_535_residues
AVASKKIPAPDEVRIKTALLSVSDKSGIVELARHLNDRGVRLVSTGGTHKALADAGLPVSDVSELTGFPEIMDGRVKTLH
PGVHGGLLAIRDDAEHAGAMSAHGITAIDLAVINLYPFEEVRAKGGDYPTTVENIDIGGPAMIRASAKNHAYVTVVTDPA
DYPLLLEEIAGGTTRYAFRQKMAAKAYARTAAYDAAISNWFAEVLDTPMPRHRVIGGVLKEEMRYGENPHQKAGFYVTGD
KRPGVATAALLQGKQLSYNNINDTDAAFELVAEFLPEKAPACAIIKHANPCGVATAPSLAEAYRRALACDSTSAFGGIIA
LNQELDAATAEEIVKLFTEVIIAPSVSDEAKAIIARKPNLRLLATGGLPDPRTPGLTAKTVAGGLLVQTRDDGMIEDIEL
KVVTKRTPTAQELEDMKFAFKVAKHVKSNAVVYAKGGQTAGIGAGQMSRVDSARIAAIKAEEAAKALGLAEPLTRGSAVA
SEAFLPFADGLLSAIAAGATAVIQPGGSMRDEEVIAAADEHNVAMVFTGMRHFRH

Specific function: De novo purine biosynthesis; ninth step. De novo purine biosynthesis; tenth step. [C]

COG id: COG0138

COG function: function code F; AICAR transformylase/IMP cyclohydrolase PurH (only IMP cyclohydrolase domain in Aful)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purH family [H]

Homologues:

Organism=Homo sapiens, GI20127454, Length=604, Percent_Identity=35.4304635761589, Blast_Score=282, Evalue=6e-76,
Organism=Escherichia coli, GI1790439, Length=538, Percent_Identity=55.3903345724907, Blast_Score=535, Evalue=1e-153,
Organism=Caenorhabditis elegans, GI71985564, Length=603, Percent_Identity=34.9917081260365, Blast_Score=289, Evalue=3e-78,
Organism=Caenorhabditis elegans, GI71985574, Length=304, Percent_Identity=31.9078947368421, Blast_Score=101, Evalue=9e-22,
Organism=Saccharomyces cerevisiae, GI6323768, Length=602, Percent_Identity=34.0531561461794, Blast_Score=286, Evalue=4e-78,
Organism=Saccharomyces cerevisiae, GI6323056, Length=600, Percent_Identity=33, Blast_Score=284, Evalue=2e-77,
Organism=Drosophila melanogaster, GI24649832, Length=601, Percent_Identity=35.6073211314476, Blast_Score=287, Evalue=1e-77,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 640 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002695
- InterPro:   IPR013982
- InterPro:   IPR016193
- InterPro:   IPR011607 [H]

Pfam domain/function: PF01808 AICARFT_IMPCHas; PF02142 MGS [H]

EC number: =2.1.2.3; =3.5.4.10 [H]

Molecular weight: Translated: 56481; Mature: 56350

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVASKKIPAPDEVRIKTALLSVSDKSGIVELARHLNDRGVRLVSTGGTHKALADAGLPV
CCCCCCCCCCCCCEEEEEEEEECCCCCCHHHHHHHCCCCCEEEEECCCCHHHHHHCCCCH
SDVSELTGFPEIMDGRVKTLHPGVHGGLLAIRDDAEHAGAMSAHGITAIDLAVINLYPFE
HHHHHHCCCHHHHCCCCEEECCCCCCCEEEEECCCHHCCCCCCCCCEEEEEEEEEECCHH
EVRAKGGDYPTTVENIDIGGPAMIRASAKNHAYVTVVTDPADYPLLLEEIAGGTTRYAFR
HHHHCCCCCCCCCCEECCCCCEEEEECCCCCEEEEEEECCCCCCHHHHHHCCCHHHHHHH
QKMAAKAYARTAAYDAAISNWFAEVLDTPMPRHRVIGGVLKEEMRYGENPHQKAGFYVTG
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCCEEEEC
DKRPGVATAALLQGKQLSYNNINDTDAAFELVAEFLPEKAPACAIIKHANPCGVATAPSL
CCCCCHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCHHH
AEAYRRALACDSTSAFGGIIALNQELDAATAEEIVKLFTEVIIAPSVSDEAKAIIARKPN
HHHHHHHHHCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCCHHEEEEECCC
LRLLATGGLPDPRTPGLTAKTVAGGLLVQTRDDGMIEDIELKVVTKRTPTAQELEDMKFA
EEEEEECCCCCCCCCCCCHHHHCCCEEEEECCCCCEEEEEEEEEECCCCCHHHHHHHHHH
FKVAKHVKSNAVVYAKGGQTAGIGAGQMSRVDSARIAAIKAEEAAKALGLAEPLTRGSAV
HHHHHHHCCCEEEEECCCCCCCCCCCHHHHCCCHHEEEEEHHHHHHHHCCHHHHHCCCHH
ASEAFLPFADGLLSAIAAGATAVIQPGGSMRDEEVIAAADEHNVAMVFTGMRHFRH
HHHCCCHHHHHHHHHHHCCCEEEECCCCCCCCCCEEEECCCCCEEEEEECHHHHCC
>Mature Secondary Structure 
AVASKKIPAPDEVRIKTALLSVSDKSGIVELARHLNDRGVRLVSTGGTHKALADAGLPV
CCCCCCCCCCCCEEEEEEEEECCCCCCHHHHHHHCCCCCEEEEECCCCHHHHHHCCCCH
SDVSELTGFPEIMDGRVKTLHPGVHGGLLAIRDDAEHAGAMSAHGITAIDLAVINLYPFE
HHHHHHCCCHHHHCCCCEEECCCCCCCEEEEECCCHHCCCCCCCCCEEEEEEEEEECCHH
EVRAKGGDYPTTVENIDIGGPAMIRASAKNHAYVTVVTDPADYPLLLEEIAGGTTRYAFR
HHHHCCCCCCCCCCEECCCCCEEEEECCCCCEEEEEEECCCCCCHHHHHHCCCHHHHHHH
QKMAAKAYARTAAYDAAISNWFAEVLDTPMPRHRVIGGVLKEEMRYGENPHQKAGFYVTG
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCCEEEEC
DKRPGVATAALLQGKQLSYNNINDTDAAFELVAEFLPEKAPACAIIKHANPCGVATAPSL
CCCCCHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCHHH
AEAYRRALACDSTSAFGGIIALNQELDAATAEEIVKLFTEVIIAPSVSDEAKAIIARKPN
HHHHHHHHHCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCCHHEEEEECCC
LRLLATGGLPDPRTPGLTAKTVAGGLLVQTRDDGMIEDIELKVVTKRTPTAQELEDMKFA
EEEEEECCCCCCCCCCCCHHHHCCCEEEEECCCCCEEEEEEEEEECCCCCHHHHHHHHHH
FKVAKHVKSNAVVYAKGGQTAGIGAGQMSRVDSARIAAIKAEEAAKALGLAEPLTRGSAV
HHHHHHHCCCEEEEECCCCCCCCCCCHHHHCCCHHEEEEEHHHHHHHHCCHHHHHCCCHH
ASEAFLPFADGLLSAIAAGATAVIQPGGSMRDEEVIAAADEHNVAMVFTGMRHFRH
HHHCCCHHHHHHHHHHHCCCEEEECCCCCCCCCCEEEECCCCCEEEEEECHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11481430 [H]