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The map label for this gene is tcyA [H]

Identifier: 150398330

GI number: 150398330

Start: 3286188

End: 3286961

Strand: Direct

Name: tcyA [H]

Synonym: Smed_3136

Alternate gene names: 150398330

Gene position: 3286188-3286961 (Clockwise)

Preceding gene: 150398326

Following gene: 150398331

Centisome position: 86.89

GC content: 60.72

Gene sequence:

>774_bases
ATGAAAATCCTTCCAACGCTTTTTGCCGCTGCCCTCCTGCAACTCGCCGCCTTCGCGCCGGCCCATGCCGGCTCCAACCT
GGAAGACGTCAAATCCGCAGGCGTTCTGAAGATCGGCACCGAGGGCAGCTATGCTCCCTTTACCTATCATGATGCGAGCG
GCGCTCTCGTCGGCTTCGACGTCGAGATCGGCCAGGAAGTTGCCAAGCGGCTCGGCGTCAAGCCGGAATTCCTCGAAGGC
AAGTGGGATGGCCTGATCGCCGGCCTCGACGCCAACCGTTACGACGCGGTCATCAACCAGGTCGGCATCACCGAGGAACG
CAAGAAGAAATACGACTTCTCCGAGCCCTATATCGCCTCCAAGGCCGTGCTGATCGTCCGCGGTGACGACGAGGAGGTCA
AGGACTTCGCCGATCTGAAGGGCAAGAAATCGGCACAGTCGCTGACCAGCAACTTCGGCAAGCTCGCACAGGCCTCGGGC
GCCGAGCTCGTGGGAACCGACGGTTTCGACCAGTCGATCCAGCTCGTGCTGACGGGCCGCGCCGACGCGACCATCAATGA
CAGCCTCTCCTTCCTCGACTTCAAGAAGCAGAAGCCGGATGCGAACGTGAAGATCGCCGCCGAAAAGCCCGACGCCGATT
ATTCCGGCATCATCATCCGCAAGGGCGAGCCGGAACTGCTCGAGGCAATCAACAAGGCGCTGGCGGAGATCAAGGCCGAC
GGCACCTATCAGAGGGTCTCGCAAAAATATTTCGGCGCCGACGTTTCGAAGTAG

Upstream 100 bases:

>100_bases
ACGGTTCGGAATTGGTCTTTTATGCGCTTGGAAACCGAACGGATTGTGCGAATCTCGCCGCAATCCGGATGACGCCGGAG
AACCAGAACTGGAATCCACG

Downstream 100 bases:

>100_bases
CGCCGCGAATTGCCTGGGGGAACGCGGCGTTCTTCCGGGACAGTTGCAGGGAACGTGGGCTTCACACCCATGCCCATCCT
GCTTATGAATCGCGATCCGT

Product: extracellular solute-binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 257; Mature: 257

Protein sequence:

>257_residues
MKILPTLFAAALLQLAAFAPAHAGSNLEDVKSAGVLKIGTEGSYAPFTYHDASGALVGFDVEIGQEVAKRLGVKPEFLEG
KWDGLIAGLDANRYDAVINQVGITEERKKKYDFSEPYIASKAVLIVRGDDEEVKDFADLKGKKSAQSLTSNFGKLAQASG
AELVGTDGFDQSIQLVLTGRADATINDSLSFLDFKKQKPDANVKIAAEKPDADYSGIIIRKGEPELLEAINKALAEIKAD
GTYQRVSQKYFGADVSK

Sequences:

>Translated_257_residues
MKILPTLFAAALLQLAAFAPAHAGSNLEDVKSAGVLKIGTEGSYAPFTYHDASGALVGFDVEIGQEVAKRLGVKPEFLEG
KWDGLIAGLDANRYDAVINQVGITEERKKKYDFSEPYIASKAVLIVRGDDEEVKDFADLKGKKSAQSLTSNFGKLAQASG
AELVGTDGFDQSIQLVLTGRADATINDSLSFLDFKKQKPDANVKIAAEKPDADYSGIIIRKGEPELLEAINKALAEIKAD
GTYQRVSQKYFGADVSK
>Mature_257_residues
MKILPTLFAAALLQLAAFAPAHAGSNLEDVKSAGVLKIGTEGSYAPFTYHDASGALVGFDVEIGQEVAKRLGVKPEFLEG
KWDGLIAGLDANRYDAVINQVGITEERKKKYDFSEPYIASKAVLIVRGDDEEVKDFADLKGKKSAQSLTSNFGKLAQASG
AELVGTDGFDQSIQLVLTGRADATINDSLSFLDFKKQKPDANVKIAAEKPDADYSGIIIRKGEPELLEAINKALAEIKAD
GTYQRVSQKYFGADVSK

Specific function: Part of the ABC transporter complex tcyABC involved in L-cystine import [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 3 family [H]

Homologues:

Organism=Escherichia coli, GI1788228, Length=234, Percent_Identity=42.3076923076923, Blast_Score=177, Evalue=5e-46,
Organism=Escherichia coli, GI1788648, Length=230, Percent_Identity=34.7826086956522, Blast_Score=122, Evalue=3e-29,
Organism=Escherichia coli, GI1787085, Length=223, Percent_Identity=34.0807174887892, Blast_Score=119, Evalue=2e-28,
Organism=Escherichia coli, GI1788649, Length=233, Percent_Identity=32.618025751073, Blast_Score=114, Evalue=9e-27,
Organism=Escherichia coli, GI1787088, Length=229, Percent_Identity=31.0043668122271, Blast_Score=100, Evalue=7e-23,
Organism=Escherichia coli, GI1787031, Length=221, Percent_Identity=29.8642533936652, Blast_Score=99, Evalue=3e-22,
Organism=Escherichia coli, GI1786876, Length=250, Percent_Identity=26.4, Blast_Score=61, Evalue=8e-11,

Paralogues:

None

Copy number: 1920 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1060 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 80 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001638
- InterPro:   IPR018313 [H]

Pfam domain/function: PF00497 SBP_bac_3 [H]

EC number: NA

Molecular weight: Translated: 27592; Mature: 27592

Theoretical pI: Translated: 4.93; Mature: 4.93

Prosite motif: PS01039 SBP_BACTERIAL_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.4 %Met     (Translated Protein)
0.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
0.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKILPTLFAAALLQLAAFAPAHAGSNLEDVKSAGVLKIGTEGSYAPFTYHDASGALVGFD
CCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHCCCEEEECCCCCCCCEEEECCCCCEEEEE
VEIGQEVAKRLGVKPEFLEGKWDGLIAGLDANRYDAVINQVGITEERKKKYDFSEPYIAS
HHHHHHHHHHHCCCCCHHCCCCCCEEECCCCCHHHHHHHHCCCCHHHHHCCCCCCCCCCC
KAVLIVRGDDEEVKDFADLKGKKSAQSLTSNFGKLAQASGAELVGTDGFDQSIQLVLTGR
CEEEEEECCCHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCEEEECCCCCCEEEEEEECC
ADATINDSLSFLDFKKQKPDANVKIAAEKPDADYSGIIIRKGEPELLEAINKALAEIKAD
CCCCCCCCHHHHHHHCCCCCCCEEEEECCCCCCCCCEEEECCCHHHHHHHHHHHHHHCCC
GTYQRVSQKYFGADVSK
CHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MKILPTLFAAALLQLAAFAPAHAGSNLEDVKSAGVLKIGTEGSYAPFTYHDASGALVGFD
CCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHCCCEEEECCCCCCCCEEEECCCCCEEEEE
VEIGQEVAKRLGVKPEFLEGKWDGLIAGLDANRYDAVINQVGITEERKKKYDFSEPYIAS
HHHHHHHHHHHCCCCCHHCCCCCCEEECCCCCHHHHHHHHCCCCHHHHHCCCCCCCCCCC
KAVLIVRGDDEEVKDFADLKGKKSAQSLTSNFGKLAQASGAELVGTDGFDQSIQLVLTGR
CEEEEEECCCHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCEEEECCCCCCEEEEEEECC
ADATINDSLSFLDFKKQKPDANVKIAAEKPDADYSGIIIRKGEPELLEAINKALAEIKAD
CCCCCCCCHHHHHHHCCCCCCCEEEEECCCCCCCCCEEEECCCHHHHHHHHHHHHHHCCC
GTYQRVSQKYFGADVSK
CHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7551042; 8969502; 9384377 [H]