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The map label for this gene is ispG [H]

Identifier: 150398327

GI number: 150398327

Start: 3282410

End: 3283663

Strand: Reverse

Name: ispG [H]

Synonym: Smed_3133

Alternate gene names: 150398327

Gene position: 3283663-3282410 (Counterclockwise)

Preceding gene: 150398328

Following gene: 150398321

Centisome position: 86.83

GC content: 63.64

Gene sequence:

>1254_bases
ATGTCTTCTGCCTTCGATTTCGAACCACAGCCGCGTCGCGCATCGGTCGCCGTCGATGTCGGCGGCGTGATCGTCGGCGG
CGGTGCCCCGGTCGTCGTCCAGTCGATGACCAACACCGACACGGCCGATATCGATGCGACCGTGGCGCAGGTGGCGGCGC
TCCACAAAGCGGGATCGGAGCTGGTGCGCATCACCGTCGACCGCGACGAGAGCGCCGCGGCCGTGCCGAAGATCCGCGAG
CGGCTCCTGCGTCTCGGCTTGGACGTGCCGCTTGTCGGCGATTTTCATTATATCGGCCACAAACTGCTTGCCGATCATCC
GGCCTGCGCAGAGGCGCTTGCAAAATACCGCATCAATCCCGGCAATGTCGGCTTCAAGGACAAGAAGGACAAGCAGTTCG
CCGAAATCGTCGAGATGGCGATCCGCTACGACAAGCCGGTGCGCATCGGGGTGAACTGGGGCTCGCTCGACCAGGAGCTC
CTGACGCGGCTGATGGACGCCAACAAGGCGAATGGCTTTCCATTGACGGCACAGCAGGTGACGCGCGAGACGATCGTGCA
GTCGGCGCTCCTTTCGGCCGAACTTGCCGAGGAGCTCGGCCTGCCTCGAAACAGGATCATTCTTTCCGCCAAGGTTTCCG
GCGTCCAGGATCTGATCGCCGTCTATGCGATGCTCTCCGCGCGTTCCGACCATGCACTTCACCTCGGGCTCACCGAGGCC
GGCATGGGAACAAAGGGTATCGTCGCTTCCTCCGCCTCGCTCGGTATTCTGATGCAGCAGGGGATCGGCGACACGATCCG
CATTTCGCTTACTCCGGAGCCGGGCGGCGACCGCACCCGCGAGGTACAGGTGGCGCAGGAGCTTCTGCAGGTGATGGGTT
TCCGCCAGTTCATCCCGGTCGTGGCGGCCTGTCCGGGGTGCGGCCGCACCACTTCCACGGTCTTTCAGGAACTTGCCCAG
AAGATTCAGGAGGATATCCGCTCGAGCATGCCGGTCTGGCGCGAGAAATATCCGGGCGTCGAGGCGCTGAAGGTCGCGGT
CATGGGCTGCATCGTCAACGGGCCCGGCGAGAGCAAGCATGCCGATATCGGAATTTCGCTTCCGGGAACCGGCGAAATGC
CGGCCGCTCCCGTCTTCATCGACGGTCACAAGGCGATGACGCTCCGCGGGCCGAAGATCGCGGAGGACTTCCAGCTTCTC
GTTGCCGATTACATCGAGAAACGCTTCGGCCACGGCCAGGCGGCGGCGGAGTAA

Upstream 100 bases:

>100_bases
CCGTTGACAAGCGGGCGTTCCTTCGCCACCTGAAAGGTGAGAATTTCGGGCGGTCCGTGCACGTTCCGCTCTGCTGCAAC
GGACCCAAGAAGAGGCCTTC

Downstream 100 bases:

>100_bases
CAGGAGCGGTTCGCCCTTTCCGAAGAGCAGTCGCGCGCGCCGGATTACGCGCGCTCACATTTCGCCGGAACGGGAGCCGA
ACGTCACGACATGATCAAGC

Product: 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase

Products: NA

Alternate protein names: 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate synthase [H]

Number of amino acids: Translated: 417; Mature: 416

Protein sequence:

>417_residues
MSSAFDFEPQPRRASVAVDVGGVIVGGGAPVVVQSMTNTDTADIDATVAQVAALHKAGSELVRITVDRDESAAAVPKIRE
RLLRLGLDVPLVGDFHYIGHKLLADHPACAEALAKYRINPGNVGFKDKKDKQFAEIVEMAIRYDKPVRIGVNWGSLDQEL
LTRLMDANKANGFPLTAQQVTRETIVQSALLSAELAEELGLPRNRIILSAKVSGVQDLIAVYAMLSARSDHALHLGLTEA
GMGTKGIVASSASLGILMQQGIGDTIRISLTPEPGGDRTREVQVAQELLQVMGFRQFIPVVAACPGCGRTTSTVFQELAQ
KIQEDIRSSMPVWREKYPGVEALKVAVMGCIVNGPGESKHADIGISLPGTGEMPAAPVFIDGHKAMTLRGPKIAEDFQLL
VADYIEKRFGHGQAAAE

Sequences:

>Translated_417_residues
MSSAFDFEPQPRRASVAVDVGGVIVGGGAPVVVQSMTNTDTADIDATVAQVAALHKAGSELVRITVDRDESAAAVPKIRE
RLLRLGLDVPLVGDFHYIGHKLLADHPACAEALAKYRINPGNVGFKDKKDKQFAEIVEMAIRYDKPVRIGVNWGSLDQEL
LTRLMDANKANGFPLTAQQVTRETIVQSALLSAELAEELGLPRNRIILSAKVSGVQDLIAVYAMLSARSDHALHLGLTEA
GMGTKGIVASSASLGILMQQGIGDTIRISLTPEPGGDRTREVQVAQELLQVMGFRQFIPVVAACPGCGRTTSTVFQELAQ
KIQEDIRSSMPVWREKYPGVEALKVAVMGCIVNGPGESKHADIGISLPGTGEMPAAPVFIDGHKAMTLRGPKIAEDFQLL
VADYIEKRFGHGQAAAE
>Mature_416_residues
SSAFDFEPQPRRASVAVDVGGVIVGGGAPVVVQSMTNTDTADIDATVAQVAALHKAGSELVRITVDRDESAAAVPKIRER
LLRLGLDVPLVGDFHYIGHKLLADHPACAEALAKYRINPGNVGFKDKKDKQFAEIVEMAIRYDKPVRIGVNWGSLDQELL
TRLMDANKANGFPLTAQQVTRETIVQSALLSAELAEELGLPRNRIILSAKVSGVQDLIAVYAMLSARSDHALHLGLTEAG
MGTKGIVASSASLGILMQQGIGDTIRISLTPEPGGDRTREVQVAQELLQVMGFRQFIPVVAACPGCGRTTSTVFQELAQK
IQEDIRSSMPVWREKYPGVEALKVAVMGCIVNGPGESKHADIGISLPGTGEMPAAPVFIDGHKAMTLRGPKIAEDFQLLV
ADYIEKRFGHGQAAAE

Specific function: Converts 2C-methyl-D-erythritol 2,4-cyclodiphosphate (ME-2,4cPP) into 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate [H]

COG id: COG0821

COG function: function code I; Enzyme involved in the deoxyxylulose pathway of isoprenoid biosynthesis

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispG family [H]

Homologues:

Organism=Escherichia coli, GI1788863, Length=382, Percent_Identity=33.5078534031414, Blast_Score=179, Evalue=4e-46,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016425
- InterPro:   IPR004588 [H]

Pfam domain/function: PF04551 GcpE [H]

EC number: =1.17.7.1 [H]

Molecular weight: Translated: 44600; Mature: 44469

Theoretical pI: Translated: 6.25; Mature: 6.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSAFDFEPQPRRASVAVDVGGVIVGGGAPVVVQSMTNTDTADIDATVAQVAALHKAGSE
CCCCCCCCCCCCCEEEEEEECCEEECCCCCEEEEECCCCCCCHHHHHHHHHHHHHHCCCE
LVRITVDRDESAAAVPKIRERLLRLGLDVPLVGDFHYIGHKLLADHPACAEALAKYRINP
EEEEEECCCCCCHHHHHHHHHHHHHCCCCCEECCHHHHHHHHHCCCHHHHHHHHHHCCCC
GNVGFKDKKDKQFAEIVEMAIRYDKPVRIGVNWGSLDQELLTRLMDANKANGFPLTAQQV
CCCCCCCCCHHHHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHCCCCCCCCCCCHHHH
TRETIVQSALLSAELAEELGLPRNRIILSAKVSGVQDLIAVYAMLSARSDHALHLGLTEA
HHHHHHHHHHHHHHHHHHHCCCHHHEEEEEECCCHHHHHHHHHHHHCCCCCEEEEEEHHC
GMGTKGIVASSASLGILMQQGIGDTIRISLTPEPGGDRTREVQVAQELLQVMGFRQFIPV
CCCCCCEEECCCHHHHHHHCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
VAACPGCGRTTSTVFQELAQKIQEDIRSSMPVWREKYPGVEALKVAVMGCIVNGPGESKH
HHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCHHHHHHHHHHHEECCCCCCCC
ADIGISLPGTGEMPAAPVFIDGHKAMTLRGPKIAEDFQLLVADYIEKRFGHGQAAAE
CEEEEECCCCCCCCCCCEEECCCEEEEECCCCHHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
SSAFDFEPQPRRASVAVDVGGVIVGGGAPVVVQSMTNTDTADIDATVAQVAALHKAGSE
CCCCCCCCCCCCEEEEEEECCEEECCCCCEEEEECCCCCCCHHHHHHHHHHHHHHCCCE
LVRITVDRDESAAAVPKIRERLLRLGLDVPLVGDFHYIGHKLLADHPACAEALAKYRINP
EEEEEECCCCCCHHHHHHHHHHHHHCCCCCEECCHHHHHHHHHCCCHHHHHHHHHHCCCC
GNVGFKDKKDKQFAEIVEMAIRYDKPVRIGVNWGSLDQELLTRLMDANKANGFPLTAQQV
CCCCCCCCCHHHHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHCCCCCCCCCCCHHHH
TRETIVQSALLSAELAEELGLPRNRIILSAKVSGVQDLIAVYAMLSARSDHALHLGLTEA
HHHHHHHHHHHHHHHHHHHCCCHHHEEEEEECCCHHHHHHHHHHHHCCCCCEEEEEEHHC
GMGTKGIVASSASLGILMQQGIGDTIRISLTPEPGGDRTREVQVAQELLQVMGFRQFIPV
CCCCCCEEECCCHHHHHHHCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
VAACPGCGRTTSTVFQELAQKIQEDIRSSMPVWREKYPGVEALKVAVMGCIVNGPGESKH
HHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCHHHHHHHHHHHEECCCCCCCC
ADIGISLPGTGEMPAAPVFIDGHKAMTLRGPKIAEDFQLLVADYIEKRFGHGQAAAE
CEEEEECCCCCCCCCCCEEECCCEEEEECCCCHHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11481430 [H]