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The map label for this gene is tbpA [H]

Identifier: 150398301

GI number: 150398301

Start: 3252828

End: 3253856

Strand: Direct

Name: tbpA [H]

Synonym: Smed_3107

Alternate gene names: 150398301

Gene position: 3252828-3253856 (Clockwise)

Preceding gene: 150398297

Following gene: 150398302

Centisome position: 86.01

GC content: 61.22

Gene sequence:

>1029_bases
ATGTCCATTTCACTCCACGGCAGAACACTTGCCGGGTTCATGATTGCAGCGGCCACCGTCACGGGCGTTTCCGCAAGTGC
TTTCGCCGCGGAAAAGACGCTGACGGTCTACACTTACGAAAGCTTCATCACAGAATGGGGGCCGGGCGCGAAGGTCTCCG
AAGCCTTCGAGAAGGTCTGCGACTGCAAGGTCGATTATGTGGGCGTCGCCGACGGGGTCGAACTGCTGACGCGGCTGAAG
CTCGAAGGTGAGGGATCCAAAGCCGACGTCGTGCTCGGTCTCGACACCAATCTCGTTGCCGAGGCCAAGGCGACCGGCTT
CTTCGTTCCGCACGGCGTCGATACCACTTCCGTCGATGTTCCTGGTGGTTTCACCGACGACACCTTCATCCCCTATGACT
ACGGCCATTTCGCCGTGGTGTACGATACCGAGATGCTGAAGAGCCCGCCGAAGAGCCTCAGGGATCTGGTGGAAGGCGAT
CCAACGCAGAAGATCGTGATCGAGGACCCGCGCACTTCCACCCCCGGCCTCGGCCTGCTGCTTTGGGTGAAATCGGTCTA
TGGCGATCGGGCCGGCGAGGCCTGGGCAAAGCTCAAGGCGCGTGTGCTGACGGTCACGCCGGGCTGGTCGGAGGCCTATG
GCCTCTTCACCAAGGGTGAGGCGCCGATGGTTCTGTCCTACACCACCTCGCCCGCATATCACATGGTCGCGGAAGATACC
GAGCGCTATCAGGCGGCCCCGTTCACCGAGGGCCACTACATCCAGATCGAAGTCGCCGCATTGACGAAGAACGCGAAGGA
CCCGGAGCTCGCCCGGAAGTTTCTGGACTTCATGATCGGTCCGGAATTCCAGTCGATCATCCCGACGACCAATTGGATGA
TGCCGGTGACGGCCACAAAGGAACCGCTGCCGGAGGCCTTCGGAAAGCTCGTCGAACCCCGGAAGACCTTTCTCATCCCC
TCCGAGGAGGTTGCGGCCAACCGCAGGGCCTGGATCGATGAGTGGCTGACGGCGATGAGCAGGAACTGA

Upstream 100 bases:

>100_bases
CCGCGGAACCTGATCCGGCTCATACCGGCGGAGGGATTAGAAGCGATCGGACCGATACGCGCTTTTCCCGTGCAGCAACG
ACAAAGGGAGAGGTGCCACA

Downstream 100 bases:

>100_bases
GGCTGGATTGTCTGTCGCCAACCAAAACAGGACGGCCGAAAACAGGATGACAATGGCCGCCGGGGGTTTCGCCCTTGGCG
GCGTTCTGCTTTTCATCGGC

Product: thiamine transporter substrate binding subunit

Products: ADP; phosphate; thiamine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 342; Mature: 341

Protein sequence:

>342_residues
MSISLHGRTLAGFMIAAATVTGVSASAFAAEKTLTVYTYESFITEWGPGAKVSEAFEKVCDCKVDYVGVADGVELLTRLK
LEGEGSKADVVLGLDTNLVAEAKATGFFVPHGVDTTSVDVPGGFTDDTFIPYDYGHFAVVYDTEMLKSPPKSLRDLVEGD
PTQKIVIEDPRTSTPGLGLLLWVKSVYGDRAGEAWAKLKARVLTVTPGWSEAYGLFTKGEAPMVLSYTTSPAYHMVAEDT
ERYQAAPFTEGHYIQIEVAALTKNAKDPELARKFLDFMIGPEFQSIIPTTNWMMPVTATKEPLPEAFGKLVEPRKTFLIP
SEEVAANRRAWIDEWLTAMSRN

Sequences:

>Translated_342_residues
MSISLHGRTLAGFMIAAATVTGVSASAFAAEKTLTVYTYESFITEWGPGAKVSEAFEKVCDCKVDYVGVADGVELLTRLK
LEGEGSKADVVLGLDTNLVAEAKATGFFVPHGVDTTSVDVPGGFTDDTFIPYDYGHFAVVYDTEMLKSPPKSLRDLVEGD
PTQKIVIEDPRTSTPGLGLLLWVKSVYGDRAGEAWAKLKARVLTVTPGWSEAYGLFTKGEAPMVLSYTTSPAYHMVAEDT
ERYQAAPFTEGHYIQIEVAALTKNAKDPELARKFLDFMIGPEFQSIIPTTNWMMPVTATKEPLPEAFGKLVEPRKTFLIP
SEEVAANRRAWIDEWLTAMSRN
>Mature_341_residues
SISLHGRTLAGFMIAAATVTGVSASAFAAEKTLTVYTYESFITEWGPGAKVSEAFEKVCDCKVDYVGVADGVELLTRLKL
EGEGSKADVVLGLDTNLVAEAKATGFFVPHGVDTTSVDVPGGFTDDTFIPYDYGHFAVVYDTEMLKSPPKSLRDLVEGDP
TQKIVIEDPRTSTPGLGLLLWVKSVYGDRAGEAWAKLKARVLTVTPGWSEAYGLFTKGEAPMVLSYTTSPAYHMVAEDTE
RYQAAPFTEGHYIQIEVAALTKNAKDPELARKFLDFMIGPEFQSIIPTTNWMMPVTATKEPLPEAFGKLVEPRKTFLIPS
EEVAANRRAWIDEWLTAMSRN

Specific function: Part of the ABC transporter complex thiBPQ involved in thiamine import [H]

COG id: COG4143

COG function: function code H; ABC-type thiamine transport system, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 1 family [H]

Homologues:

Organism=Escherichia coli, GI1786255, Length=312, Percent_Identity=57.3717948717949, Blast_Score=380, Evalue=1e-107,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059
- InterPro:   IPR005948
- InterPro:   IPR005967 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 37399; Mature: 37268

Theoretical pI: Translated: 4.64; Mature: 4.64

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSISLHGRTLAGFMIAAATVTGVSASAFAAEKTLTVYTYESFITEWGPGAKVSEAFEKVC
CEEEECCCHHHHHHHHHHHHHCCCHHHHHHCCEEEEEEEHHHHHHCCCCCHHHHHHHHHH
DCKVDYVGVADGVELLTRLKLEGEGSKADVVLGLDTNLVAEAKATGFFVPHGVDTTSVDV
CCCCEEEECHHHHHHHHHHEECCCCCCEEEEEECCCCEEEHHCCCEEEECCCCCCCEEEC
PGGFTDDTFIPYDYGHFAVVYDTEMLKSPPKSLRDLVEGDPTQKIVIEDPRTSTPGLGLL
CCCCCCCCCCCCCCCCEEEEEEHHHHHCCCHHHHHHHCCCCCCEEEEECCCCCCCCCHHH
LWVKSVYGDRAGEAWAKLKARVLTVTPGWSEAYGLFTKGEAPMVLSYTTSPAYHMVAEDT
HHHHHHHCCCCHHHHHEEEEEEEEECCCCCHHCCEEECCCCCEEEEEECCCCEEEECCCH
ERYQAAPFTEGHYIQIEVAALTKNAKDPELARKFLDFMIGPEFQSIIPTTNWMMPVTATK
HHHCCCCCCCCCEEEEEEEEECCCCCCHHHHHHHHHHHCCCCHHHHCCCCCEEEEECCCC
EPLPEAFGKLVEPRKTFLIPSEEVAANRRAWIDEWLTAMSRN
CHHHHHHHHHCCCCCEEECCCHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SISLHGRTLAGFMIAAATVTGVSASAFAAEKTLTVYTYESFITEWGPGAKVSEAFEKVC
EEEECCCHHHHHHHHHHHHHCCCHHHHHHCCEEEEEEEHHHHHHCCCCCHHHHHHHHHH
DCKVDYVGVADGVELLTRLKLEGEGSKADVVLGLDTNLVAEAKATGFFVPHGVDTTSVDV
CCCCEEEECHHHHHHHHHHEECCCCCCEEEEEECCCCEEEHHCCCEEEECCCCCCCEEEC
PGGFTDDTFIPYDYGHFAVVYDTEMLKSPPKSLRDLVEGDPTQKIVIEDPRTSTPGLGLL
CCCCCCCCCCCCCCCCEEEEEEHHHHHCCCHHHHHHHCCCCCCEEEEECCCCCCCCCHHH
LWVKSVYGDRAGEAWAKLKARVLTVTPGWSEAYGLFTKGEAPMVLSYTTSPAYHMVAEDT
HHHHHHHCCCCHHHHHEEEEEEEEECCCCCHHCCEEECCCCCEEEEEECCCCEEEECCCH
ERYQAAPFTEGHYIQIEVAALTKNAKDPELARKFLDFMIGPEFQSIIPTTNWMMPVTATK
HHHCCCCCCCCCEEEEEEEEECCCCCCHHHHHHHHHHHCCCCHHHHCCCCCEEEEECCCC
EPLPEAFGKLVEPRKTFLIPSEEVAANRRAWIDEWLTAMSRN
CHHHHHHHHHCCCCCEEECCCHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; thiamine [Periplasm]; H2O [C]

Specific reaction: ATP + thiamine [Periplasm] + H2O = ADP + phosphate + thiamine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA