Click here to switch to the map view.

The map label for this gene is thiP [H]

Identifier: 150398302

GI number: 150398302

Start: 3253909

End: 3255504

Strand: Direct

Name: thiP [H]

Synonym: Smed_3108

Alternate gene names: 150398302

Gene position: 3253909-3255504 (Clockwise)

Preceding gene: 150398301

Following gene: 150398303

Centisome position: 86.04

GC content: 65.35

Gene sequence:

>1596_bases
ATGGCCGCCGGGGGTTTCGCCCTTGGCGGCGTTCTGCTTTTCATCGGCCTCGCAGCCGGGTCGCTGCTCGCCCAATCGAC
CGGCGGCAACGATGCCGGTCCGTTCCTTGACGCCTATATCTGGCGCATAACACGCTTCACACTGCTGCAGGCAGGCCTTT
CCACGCTCTTTTCCGTGCTGCTCGCCATACCGGTGGCCCGCGCACTTGCCCGCCAGGCTTCGTTCCCCGGCCGCATCTGG
ATGTTGCGGCTGCTGGCGCTGCCTCTCGGCCTTCCGGCCCTCGTCGGCGCTCTCGGCCTCATTGAGATCTGGGGACGACA
GGGGCTTTTGAACAGGGTGCTCGCGGCGGCCGGGCTGGAGCAACCGGTCAGCATCTATGGGCTCTCCGGCATTCTCATCG
CCCATGTCTTTTTCAACATGCCGCTCGCCGCCCGTCTGATGCTTGCCGGCATCGAGCGCATTCCGGCCGAGTATTGGCGC
ACCTCCGCCAATCTCGGCATGAGCGCCTTCGCCATCTTCCGCTTCATCGAGTGGCCGGTGATCCGCGGATTGCTTCCCGG
CATCGCCGGACTCGTCTTCATGCTCTGCGCGACCAGCTTCACACTCGTGCTGACGCTTGGTGGCGGTCCGGCCGCCAGCA
CCATCGAAGTGGCCATCTATCAGGCGCTGCGCTTCGATTTCGACCCGCCGCGAGCGATCGCCCTGGCGGCACTTCAGGTC
GCCCTTACAGGTGCGCTGCTTCTCGTCCTGAAATTGATCGCGCCGCCGCCGCCGGAGGGTGAGACGAGCGGCAAGTCGAT
CCGGCGTTCCGACGGTGCAAGCAGTCTTTCCCGGCTTGCCGACCGGGTTTGGCTGGTCCTCGCCGCCGGCCTGGTAGGCC
TGCCGTTTGCAGCCATCGCCTATGCCGGTTCGAAGGCTGATCTGTTCCGGCTTCTGAGCGAGCCGGTGTTCCATCGGGCG
CTCGCGATGAGCGCGACCATCTCGGTACTCTCCGCCGTCATTTCGGTCGCTGTGACGGCGCTGATGATCCGCACCGAAAG
TCTCGCACTGGCGCAGCGGCGCCCCGGAATGACGGCACGTTTTTTCGCCGGCGCTATCGGTGCGAGCACATCCTTTATCC
TGCTGATACCACCCGTCGTGCTCGGCGCCGGGTGGTTCCTGCTGTTGAGGCCTTTCGGCGACGTCGCCCGCTTCGCCCCT
GCCGTCGTCGTCGCCATCAACGCGCTCATGGCGCTTCCCTTCGTTCACCGGGTGCTCGCACCCGCCATGGCGACGCACGC
CGTGCGCACCGGTCGCCTTGCCGCAAGCCTTGGAATCGCCGGCTTTCAACGCCTGCTATGGATCGACTGGCCTGGCCTGC
GCAAACCCCTTTTCGTCGCATTCTCCTTTGCCATGGCCCTGTCCCTGGGGGATCTGGGAGCGGTCGCACTTTTCGGCTCG
CAGGACATGGTAACCCTCCCTTATCTGCTCTATAGCCGGATGGGCAGCTATCGCACCGCCGATGCGGCCGGGCTCGCGCT
GTTCCTAGGCCTCCTCTGCCTGGTCCTGACGGTGCTCGGCACGGCGGGAGAAGAGCCTGAAGGTAAAGGCGCATGA

Upstream 100 bases:

>100_bases
CGCAGGGCCTGGATCGATGAGTGGCTGACGGCGATGAGCAGGAACTGAGGCTGGATTGTCTGTCGCCAACCAAAACAGGA
CGGCCGAAAACAGGATGACA

Downstream 100 bases:

>100_bases
TTTCCACCGCTCTCGCCCTCAGGGGCGTCGAGATAAGCTTTGACGCGACGACGCTCGCCTTCGACTGCACCATTGCCGCG
GGCGGTATCGTCGCGGTCGC

Product: thiamine transporter membrane protein

Products: ADP; phosphate; thiamine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 531; Mature: 530

Protein sequence:

>531_residues
MAAGGFALGGVLLFIGLAAGSLLAQSTGGNDAGPFLDAYIWRITRFTLLQAGLSTLFSVLLAIPVARALARQASFPGRIW
MLRLLALPLGLPALVGALGLIEIWGRQGLLNRVLAAAGLEQPVSIYGLSGILIAHVFFNMPLAARLMLAGIERIPAEYWR
TSANLGMSAFAIFRFIEWPVIRGLLPGIAGLVFMLCATSFTLVLTLGGGPAASTIEVAIYQALRFDFDPPRAIALAALQV
ALTGALLLVLKLIAPPPPEGETSGKSIRRSDGASSLSRLADRVWLVLAAGLVGLPFAAIAYAGSKADLFRLLSEPVFHRA
LAMSATISVLSAVISVAVTALMIRTESLALAQRRPGMTARFFAGAIGASTSFILLIPPVVLGAGWFLLLRPFGDVARFAP
AVVVAINALMALPFVHRVLAPAMATHAVRTGRLAASLGIAGFQRLLWIDWPGLRKPLFVAFSFAMALSLGDLGAVALFGS
QDMVTLPYLLYSRMGSYRTADAAGLALFLGLLCLVLTVLGTAGEEPEGKGA

Sequences:

>Translated_531_residues
MAAGGFALGGVLLFIGLAAGSLLAQSTGGNDAGPFLDAYIWRITRFTLLQAGLSTLFSVLLAIPVARALARQASFPGRIW
MLRLLALPLGLPALVGALGLIEIWGRQGLLNRVLAAAGLEQPVSIYGLSGILIAHVFFNMPLAARLMLAGIERIPAEYWR
TSANLGMSAFAIFRFIEWPVIRGLLPGIAGLVFMLCATSFTLVLTLGGGPAASTIEVAIYQALRFDFDPPRAIALAALQV
ALTGALLLVLKLIAPPPPEGETSGKSIRRSDGASSLSRLADRVWLVLAAGLVGLPFAAIAYAGSKADLFRLLSEPVFHRA
LAMSATISVLSAVISVAVTALMIRTESLALAQRRPGMTARFFAGAIGASTSFILLIPPVVLGAGWFLLLRPFGDVARFAP
AVVVAINALMALPFVHRVLAPAMATHAVRTGRLAASLGIAGFQRLLWIDWPGLRKPLFVAFSFAMALSLGDLGAVALFGS
QDMVTLPYLLYSRMGSYRTADAAGLALFLGLLCLVLTVLGTAGEEPEGKGA
>Mature_530_residues
AAGGFALGGVLLFIGLAAGSLLAQSTGGNDAGPFLDAYIWRITRFTLLQAGLSTLFSVLLAIPVARALARQASFPGRIWM
LRLLALPLGLPALVGALGLIEIWGRQGLLNRVLAAAGLEQPVSIYGLSGILIAHVFFNMPLAARLMLAGIERIPAEYWRT
SANLGMSAFAIFRFIEWPVIRGLLPGIAGLVFMLCATSFTLVLTLGGGPAASTIEVAIYQALRFDFDPPRAIALAALQVA
LTGALLLVLKLIAPPPPEGETSGKSIRRSDGASSLSRLADRVWLVLAAGLVGLPFAAIAYAGSKADLFRLLSEPVFHRAL
AMSATISVLSAVISVAVTALMIRTESLALAQRRPGMTARFFAGAIGASTSFILLIPPVVLGAGWFLLLRPFGDVARFAPA
VVVAINALMALPFVHRVLAPAMATHAVRTGRLAASLGIAGFQRLLWIDWPGLRKPLFVAFSFAMALSLGDLGAVALFGSQ
DMVTLPYLLYSRMGSYRTADAAGLALFLGLLCLVLTVLGTAGEEPEGKGA

Specific function: Part of the ABC transporter complex thiBPQ involved in thiamine import. Probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG1178

COG function: function code P; ABC-type Fe3+ transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 ABC transmembrane type-1 domains [H]

Homologues:

Organism=Escherichia coli, GI1786254, Length=468, Percent_Identity=43.1623931623932, Blast_Score=320, Evalue=1e-88,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515
- InterPro:   IPR005947 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 55707; Mature: 55576

Theoretical pI: Translated: 10.97; Mature: 10.97

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAAGGFALGGVLLFIGLAAGSLLAQSTGGNDAGPFLDAYIWRITRFTLLQAGLSTLFSVL
CCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
LAIPVARALARQASFPGRIWMLRLLALPLGLPALVGALGLIEIWGRQGLLNRVLAAAGLE
HHHHHHHHHHHHCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCHHHHHHHHHHCCC
QPVSIYGLSGILIAHVFFNMPLAARLMLAGIERIPAEYWRTSANLGMSAFAIFRFIEWPV
CCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCHHHHHHHCCCCHHHHHHHHHHHHHH
IRGLLPGIAGLVFMLCATSFTLVLTLGGGPAASTIEVAIYQALRFDFDPPRAIALAALQV
HHHHHHHHHHHHHHHHHHHHHEEEEECCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHH
ALTGALLLVLKLIAPPPPEGETSGKSIRRSDGASSLSRLADRVWLVLAAGLVGLPFAAIA
HHHHHHHHHHHHHCCCCCCCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YAGSKADLFRLLSEPVFHRALAMSATISVLSAVISVAVTALMIRTESLALAQRRPGMTAR
HCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHH
FFAGAIGASTSFILLIPPVVLGAGWFLLLRPFGDVARFAPAVVVAINALMALPFVHRVLA
HHHHHHCCCCCEEEEECHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
PAMATHAVRTGRLAASLGIAGFQRLLWIDWPGLRKPLFVAFSFAMALSLGDLGAVALFGS
HHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCHHHHHHHHHHHHHHHCCCCCEEEECC
QDMVTLPYLLYSRMGSYRTADAAGLALFLGLLCLVLTVLGTAGEEPEGKGA
CCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure 
AAGGFALGGVLLFIGLAAGSLLAQSTGGNDAGPFLDAYIWRITRFTLLQAGLSTLFSVL
CCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
LAIPVARALARQASFPGRIWMLRLLALPLGLPALVGALGLIEIWGRQGLLNRVLAAAGLE
HHHHHHHHHHHHCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCHHHHHHHHHHCCC
QPVSIYGLSGILIAHVFFNMPLAARLMLAGIERIPAEYWRTSANLGMSAFAIFRFIEWPV
CCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCHHHHHHHCCCCHHHHHHHHHHHHHH
IRGLLPGIAGLVFMLCATSFTLVLTLGGGPAASTIEVAIYQALRFDFDPPRAIALAALQV
HHHHHHHHHHHHHHHHHHHHHEEEEECCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHH
ALTGALLLVLKLIAPPPPEGETSGKSIRRSDGASSLSRLADRVWLVLAAGLVGLPFAAIA
HHHHHHHHHHHHHCCCCCCCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YAGSKADLFRLLSEPVFHRALAMSATISVLSAVISVAVTALMIRTESLALAQRRPGMTAR
HCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHH
FFAGAIGASTSFILLIPPVVLGAGWFLLLRPFGDVARFAPAVVVAINALMALPFVHRVLA
HHHHHHCCCCCEEEEECHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
PAMATHAVRTGRLAASLGIAGFQRLLWIDWPGLRKPLFVAFSFAMALSLGDLGAVALFGS
HHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCHHHHHHHHHHHHHHHCCCCCEEEECC
QDMVTLPYLLYSRMGSYRTADAAGLALFLGLLCLVLTVLGTAGEEPEGKGA
CCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; thiamine [Periplasm]; H2O [C]

Specific reaction: ATP + thiamine [Periplasm] + H2O = ADP + phosphate + thiamine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA