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The map label for this gene is tesA [C]

Identifier: 150398267

GI number: 150398267

Start: 3220815

End: 3221456

Strand: Reverse

Name: tesA [C]

Synonym: Smed_3073

Alternate gene names: 150398267

Gene position: 3221456-3220815 (Counterclockwise)

Preceding gene: 150398272

Following gene: 150398266

Centisome position: 85.18

GC content: 59.81

Gene sequence:

>642_bases
ATGGCATTAAAAGACTTTCTGCCCTTTTTATTCGGCCTGACAATGACAATCGTGTTATCGGCGGCGGCTCGTGCCGAGCC
CGTAAGCCTCGTCGGCTTCGGCGACAGCCTCATGGCCGGCTATCAGCTTCCCCCTGAAGATGCTTTTCCCACGCGTCTCG
AAAAGGCCCTGAAAGGCAAGGGAATCGATGTCACGATCGCCAATGCCGGCGTTTCAGGGGACACGACCTCCGGCGGCCTC
GCCCGCATAGACTGGTCGGTCCCGGAGGGAACCGACGGAGTGATCCTGGAGCTCGGGGCCAATGATGCGTTACGCGGCAT
CCCGCCGGAAGAGACGCGCAAAAATCTCGAAGCAATGATCGTGCGGCTCAAGAAGCGCGGCATCGCAGTCCTGCTTGCTG
GGATGATGGCGCCGCCGAACATGGGGCCGGATTATGGGGAGCGCTTCAACGCCATCTATCCCGAGCTCGCCAAGGAGCAT
GACACCGTTTTCTACCCCTTCTTTCTCGATGGCGTCGTCACCGAGGCCGGTCTGAAGCTAGAAGACGGAATGCATCCGAA
CGGCGAGGGTATCGCCGTAATGGTCGATCGCTTCCTGCCGACGGCCGAGACCTTCATCCGCACACTTCAGGAAGGAGAGT
GA

Upstream 100 bases:

>100_bases
TTTTTCAACTCGATGATGGTTTTTGCCACGCGAAAGAGCCCTATATGAAAATCTGATACGGATCACGAAATGAATTGCAC
GCCGATTTAGGAACGAACCT

Downstream 100 bases:

>100_bases
CGCCAGCGCTTAGCGACATCCACAGTTAAGATAAAGTTAACGTTCCTACGGAACAAATAATGGTTGCGTGCAGGTGCGAT
GCGTGATTCGCTTCAAGCAT

Product: GDSL family lipase

Products: NA

Alternate protein names: Aryl-ester hydrolase [H]

Number of amino acids: Translated: 213; Mature: 212

Protein sequence:

>213_residues
MALKDFLPFLFGLTMTIVLSAAARAEPVSLVGFGDSLMAGYQLPPEDAFPTRLEKALKGKGIDVTIANAGVSGDTTSGGL
ARIDWSVPEGTDGVILELGANDALRGIPPEETRKNLEAMIVRLKKRGIAVLLAGMMAPPNMGPDYGERFNAIYPELAKEH
DTVFYPFFLDGVVTEAGLKLEDGMHPNGEGIAVMVDRFLPTAETFIRTLQEGE

Sequences:

>Translated_213_residues
MALKDFLPFLFGLTMTIVLSAAARAEPVSLVGFGDSLMAGYQLPPEDAFPTRLEKALKGKGIDVTIANAGVSGDTTSGGL
ARIDWSVPEGTDGVILELGANDALRGIPPEETRKNLEAMIVRLKKRGIAVLLAGMMAPPNMGPDYGERFNAIYPELAKEH
DTVFYPFFLDGVVTEAGLKLEDGMHPNGEGIAVMVDRFLPTAETFIRTLQEGE
>Mature_212_residues
ALKDFLPFLFGLTMTIVLSAAARAEPVSLVGFGDSLMAGYQLPPEDAFPTRLEKALKGKGIDVTIANAGVSGDTTSGGLA
RIDWSVPEGTDGVILELGANDALRGIPPEETRKNLEAMIVRLKKRGIAVLLAGMMAPPNMGPDYGERFNAIYPELAKEHD
TVFYPFFLDGVVTEAGLKLEDGMHPNGEGIAVMVDRFLPTAETFIRTLQEGE

Specific function: Favors the hydrolysis of several arylesters [H]

COG id: COG2755

COG function: function code E; Lysophospholipase L1 and related esterases

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 'GDSL' lipolytic enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1786702, Length=191, Percent_Identity=36.6492146596859, Blast_Score=120, Evalue=7e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013830
- InterPro:   IPR013831
- InterPro:   IPR001087
- InterPro:   IPR008265 [H]

Pfam domain/function: PF00657 Lipase_GDSL [H]

EC number: =3.1.1.2 [H]

Molecular weight: Translated: 22854; Mature: 22722

Theoretical pI: Translated: 4.39; Mature: 4.39

Prosite motif: PS01098 LIPASE_GDSL_SER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALKDFLPFLFGLTMTIVLSAAARAEPVSLVGFGDSLMAGYQLPPEDAFPTRLEKALKGK
CCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCHHHHCCCCCCCCCCCHHHHHHHHCCC
GIDVTIANAGVSGDTTSGGLARIDWSVPEGTDGVILELGANDALRGIPPEETRKNLEAMI
CCEEEEECCCCCCCCCCCCEEEEEECCCCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHH
VRLKKRGIAVLLAGMMAPPNMGPDYGERFNAIYPELAKEHDTVFYPFFLDGVVTEAGLKL
HHHHHCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHCCCEE
EDGMHPNGEGIAVMVDRFLPTAETFIRTLQEGE
CCCCCCCCCEEEEEEEHHCCCHHHHHHHHHCCC
>Mature Secondary Structure 
ALKDFLPFLFGLTMTIVLSAAARAEPVSLVGFGDSLMAGYQLPPEDAFPTRLEKALKGK
CHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCHHHHCCCCCCCCCCCHHHHHHHHCCC
GIDVTIANAGVSGDTTSGGLARIDWSVPEGTDGVILELGANDALRGIPPEETRKNLEAMI
CCEEEEECCCCCCCCCCCCEEEEEECCCCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHH
VRLKKRGIAVLLAGMMAPPNMGPDYGERFNAIYPELAKEHDTVFYPFFLDGVVTEAGLKL
HHHHHCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHCCCEE
EDGMHPNGEGIAVMVDRFLPTAETFIRTLQEGE
CCCCCCCCCEEEEEEEHHCCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8141782 [H]