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The map label for this gene is bkdA2 [H]

Identifier: 150398025

GI number: 150398025

Start: 2951735

End: 2952748

Strand: Direct

Name: bkdA2 [H]

Synonym: Smed_2827

Alternate gene names: 150398025

Gene position: 2951735-2952748 (Clockwise)

Preceding gene: 150398024

Following gene: 150398026

Centisome position: 78.05

GC content: 64.79

Gene sequence:

>1014_bases
ATGGCCAGAATGACGATGATCGAAGCGGTGCGCAGCGCCATGGACGTGTCGATGGCGCGCGACGAGGACGTCGTCGTCTT
CGGCGAGGACGTCGGCTATTTCGGCGGCGTCTTCCGCTGCACCCAGGGGCTTCAGGCCAAATACGGCAAGACCCGCTGCT
TCGACGCACCGATAAGCGAATCCGGCATCGTCGGCACGGCCATCGGCATGGCCGCCTACGGGCTGAAACCCTGCGTCGAG
ATTCAGTTCGCCGATTACATGTATCCGGCCTACGATCAGCTTACCCAGGAGGCCGCACGCATCCGCTATCGCTCGAACGG
CGACTTCACCTGCCCGATCGTCGTCCGAATGCCGACAGGCGGCGGCATCTTCGGCGGCCAGACCCATAGCCAGAGCCCGG
AGGCGCTCTTCACCCATGTGTGCGGGCTCAAGGTGGTCGTACCGTCGACGCCATATGACGCCAAGGGACTGCTGATCTCG
GCGATCGAGGATCCGGATCCGGTGATGTTCCTCGAGCCGAAGCGCCTCTATAACGGTCCCTTCGACGGCCACCACGAACG
GCCGGTCACGGCATGGTCCAGGCACGAGCTCGGAGAGGTTCCGGACGGGCATTACACGATCCCGATCGGCAAGGCCGAGA
TTCGCCGCAAGGGCTCGGGCGTGACGGTGATCGCCTACGGAACCATGGTGCATGTGGCTCTTGCCGCGACCGAGGAGACC
GGCATCGACGCGGAAGTGATCGACCTGCGAAGTCTCTTGCCGCTCGACCTCGAGACGATCGTGCAATCGGTGAGCAAGAC
GGGGCGCTGCGTGGTTGTGCACGAGGCGACGCTGACGTCCGGCTTCGGCGCAGAACTCGTGGCTCTTGTCCAGGAGCACT
GCTTCTACCACCTCGAAGCGCCGGTCGTGCGCGTCACGGGCTGGGATACCCCCTATCCGCACGCCCAGGAATGGGACTAT
TTCCCAGGCCCGGCACGCGTCGGGCGCGCGCTCGCCGAAGCGATGGAGGGCTGA

Upstream 100 bases:

>100_bases
CATGCCGGCGGCAGGCCGTCGGTGCGGGACATCTTCGAAGGCGTCTATGCCGAGATGCCGCCCCATATCCGCCGCCAGCG
GCAGAAAGCGGGGTACTGAC

Downstream 100 bases:

>100_bases
CGCATGGGCGAATTCACCATCAAGATGCCGGATGTCGGCGAAGGCGTCGCGGAGGCCGAACTCGTCGAATGGCATGTGAA
GCCGGGAGACCCGGTACGCG

Product: transketolase central region

Products: NA

Alternate protein names: Branched-chain alpha-keto acid dehydrogenase E1 component beta chain; BCKDH E1-beta [H]

Number of amino acids: Translated: 337; Mature: 336

Protein sequence:

>337_residues
MARMTMIEAVRSAMDVSMARDEDVVVFGEDVGYFGGVFRCTQGLQAKYGKTRCFDAPISESGIVGTAIGMAAYGLKPCVE
IQFADYMYPAYDQLTQEAARIRYRSNGDFTCPIVVRMPTGGGIFGGQTHSQSPEALFTHVCGLKVVVPSTPYDAKGLLIS
AIEDPDPVMFLEPKRLYNGPFDGHHERPVTAWSRHELGEVPDGHYTIPIGKAEIRRKGSGVTVIAYGTMVHVALAATEET
GIDAEVIDLRSLLPLDLETIVQSVSKTGRCVVVHEATLTSGFGAELVALVQEHCFYHLEAPVVRVTGWDTPYPHAQEWDY
FPGPARVGRALAEAMEG

Sequences:

>Translated_337_residues
MARMTMIEAVRSAMDVSMARDEDVVVFGEDVGYFGGVFRCTQGLQAKYGKTRCFDAPISESGIVGTAIGMAAYGLKPCVE
IQFADYMYPAYDQLTQEAARIRYRSNGDFTCPIVVRMPTGGGIFGGQTHSQSPEALFTHVCGLKVVVPSTPYDAKGLLIS
AIEDPDPVMFLEPKRLYNGPFDGHHERPVTAWSRHELGEVPDGHYTIPIGKAEIRRKGSGVTVIAYGTMVHVALAATEET
GIDAEVIDLRSLLPLDLETIVQSVSKTGRCVVVHEATLTSGFGAELVALVQEHCFYHLEAPVVRVTGWDTPYPHAQEWDY
FPGPARVGRALAEAMEG
>Mature_336_residues
ARMTMIEAVRSAMDVSMARDEDVVVFGEDVGYFGGVFRCTQGLQAKYGKTRCFDAPISESGIVGTAIGMAAYGLKPCVEI
QFADYMYPAYDQLTQEAARIRYRSNGDFTCPIVVRMPTGGGIFGGQTHSQSPEALFTHVCGLKVVVPSTPYDAKGLLISA
IEDPDPVMFLEPKRLYNGPFDGHHERPVTAWSRHELGEVPDGHYTIPIGKAEIRRKGSGVTVIAYGTMVHVALAATEETG
IDAEVIDLRSLLPLDLETIVQSVSKTGRCVVVHEATLTSGFGAELVALVQEHCFYHLEAPVVRVTGWDTPYPHAQEWDYF
PGPARVGRALAEAMEG

Specific function: The branched-chain alpha-keto dehydrogenase complex catalyzes the overall conversion of alpha-keto acids to acyl-CoA and CO(2). It contains multiple copies of three enzymatic components:branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltran

COG id: COG0022

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dehydrogenase (E1) component, eukaryotic type, beta subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Homo sapiens, GI4557353, Length=339, Percent_Identity=48.377581120944, Blast_Score=315, Evalue=5e-86,
Organism=Homo sapiens, GI34101272, Length=339, Percent_Identity=48.377581120944, Blast_Score=315, Evalue=5e-86,
Organism=Homo sapiens, GI156564403, Length=318, Percent_Identity=39.937106918239, Blast_Score=209, Evalue=2e-54,
Organism=Homo sapiens, GI291084858, Length=318, Percent_Identity=38.0503144654088, Blast_Score=190, Evalue=2e-48,
Organism=Caenorhabditis elegans, GI17506935, Length=339, Percent_Identity=48.377581120944, Blast_Score=298, Evalue=2e-81,
Organism=Caenorhabditis elegans, GI17538422, Length=339, Percent_Identity=39.5280235988201, Blast_Score=219, Evalue=2e-57,
Organism=Saccharomyces cerevisiae, GI6319698, Length=326, Percent_Identity=39.2638036809816, Blast_Score=224, Evalue=1e-59,
Organism=Drosophila melanogaster, GI160714832, Length=339, Percent_Identity=48.0825958702065, Blast_Score=312, Evalue=2e-85,
Organism=Drosophila melanogaster, GI160714828, Length=339, Percent_Identity=48.0825958702065, Blast_Score=312, Evalue=2e-85,
Organism=Drosophila melanogaster, GI21358145, Length=318, Percent_Identity=39.937106918239, Blast_Score=223, Evalue=1e-58,
Organism=Drosophila melanogaster, GI24650940, Length=318, Percent_Identity=39.937106918239, Blast_Score=223, Evalue=1e-58,
Organism=Drosophila melanogaster, GI24650943, Length=90, Percent_Identity=38.8888888888889, Blast_Score=78, Evalue=9e-15,
Organism=Drosophila melanogaster, GI24650945, Length=90, Percent_Identity=38.8888888888889, Blast_Score=78, Evalue=9e-15,
Organism=Drosophila melanogaster, GI160714826, Length=66, Percent_Identity=48.4848484848485, Blast_Score=74, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009014
- InterPro:   IPR015941
- InterPro:   IPR005475
- InterPro:   IPR005476 [H]

Pfam domain/function: PF02779 Transket_pyr; PF02780 Transketolase_C [H]

EC number: =1.2.4.4 [H]

Molecular weight: Translated: 36704; Mature: 36573

Theoretical pI: Translated: 5.08; Mature: 5.08

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARMTMIEAVRSAMDVSMARDEDVVVFGEDVGYFGGVFRCTQGLQAKYGKTRCFDAPISE
CCHHHHHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHHHHCCCCCEEECCCCCC
SGIVGTAIGMAAYGLKPCVEIQFADYMYPAYDQLTQEAARIRYRSNGDFTCPIVVRMPTG
CCCCHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHEEECCCCCEEEEEEEEECCC
GGIFGGQTHSQSPEALFTHVCGLKVVVPSTPYDAKGLLISAIEDPDPVMFLEPKRLYNGP
CCCCCCCCCCCCHHHHHHHHCCCEEEECCCCCCCCCEEEEECCCCCCEEEECCHHHHCCC
FDGHHERPVTAWSRHELGEVPDGHYTIPIGKAEIRRKGSGVTVIAYGTMVHVALAATEET
CCCCCCCCCCCCCHHCCCCCCCCCEEEEECHHHHHHCCCCEEEEEECCEEEEEEEECCCC
GIDAEVIDLRSLLPLDLETIVQSVSKTGRCVVVHEATLTSGFGAELVALVQEHCFYHLEA
CCCHHHHHHHHHCCCCHHHHHHHHHCCCCEEEEEECEECCCCHHHHHHHHHHHHEEEECC
PVVRVTGWDTPYPHAQEWDYFPGPARVGRALAEAMEG
CEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure 
ARMTMIEAVRSAMDVSMARDEDVVVFGEDVGYFGGVFRCTQGLQAKYGKTRCFDAPISE
CHHHHHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHHHHCCCCCEEECCCCCC
SGIVGTAIGMAAYGLKPCVEIQFADYMYPAYDQLTQEAARIRYRSNGDFTCPIVVRMPTG
CCCCHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHEEECCCCCEEEEEEEEECCC
GGIFGGQTHSQSPEALFTHVCGLKVVVPSTPYDAKGLLISAIEDPDPVMFLEPKRLYNGP
CCCCCCCCCCCCHHHHHHHHCCCEEEECCCCCCCCCEEEEECCCCCCEEEECCHHHHCCC
FDGHHERPVTAWSRHELGEVPDGHYTIPIGKAEIRRKGSGVTVIAYGTMVHVALAATEET
CCCCCCCCCCCCCHHCCCCCCCCCEEEEECHHHHHHCCCCEEEEEECCEEEEEEEECCCC
GIDAEVIDLRSLLPLDLETIVQSVSKTGRCVVVHEATLTSGFGAELVALVQEHCFYHLEA
CCCHHHHHHHHHCCCCHHHHHHHHHCCCCEEEEEECEECCCCHHHHHHHHHHHHEEEECC
PVVRVTGWDTPYPHAQEWDYFPGPARVGRALAEAMEG
CEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]