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The map label for this gene is bkdA1 [H]

Identifier: 150398024

GI number: 150398024

Start: 2950501

End: 2951733

Strand: Direct

Name: bkdA1 [H]

Synonym: Smed_2826

Alternate gene names: 150398024

Gene position: 2950501-2951733 (Clockwise)

Preceding gene: 150398023

Following gene: 150398025

Centisome position: 78.02

GC content: 63.75

Gene sequence:

>1233_bases
ATGGATGAATTCAGCCGACTGAGCCTGCATGTCCCCGAACCGGCCGTCCGGCCGGGCGATCAGCCCGACTTTTCCAATGT
GAAGATTCCGAAGGCGGGCTCGGTGCCGCGGACGGAAGTCGACGTGGACCCCGAAGAGATCCGCGACCTCGCCTATTCGA
TCATCCGGGTGCTTAATCGCGAGGGCGAGGCGGTCGGACCCTGGGCAGGCCTTCTTTCGGACGAGGAACTACTGACCGGC
CTCAGGCATATGATGCTGCTGAGGGCCTTCGACGCGCGTATGCTCATGGCGCAGCGCCAGGGCAAGACGTCCTTCTACAT
GCAGCATCTCGGCGAAGAGGCGGTGAGCTGTGCATTCCGCAAGGCGCTCCGCAAGGGCGACATGAATTTTCCGACCTACC
GTCAGGCGGGGCTTCTGATCGCCGACGACTACCCGATGGTCGAGATGATGAACCAGATCTTCTCGAACGAGCTCGATCCC
TGCCATGGGCGGCAGTTGCCGGTCATGTACACCTCCAAGGCGCACGGCTTCTTCACGATATCCGGCAACCTCGCCACGCA
ATATGTCCAGGCGGTCGGCTGGGCGATGGCGTCCGCCATCAAGAACGATACCCGGATCGCCGCCGGCTGGATCGGCGACG
GCTCGACGGCCGAGTCGGACTTCCATTCCGCGCTGGTCTTCGCCTCCACCTACAAGGCGCCCGTCATTCTCAACATCGTC
AACAATCAATGGGCTATCTCGACCTTCCAGGGTATCGCCCGAGGGGGCTCCGGCACATTTGCGGCCCGGGGTCTGGGTTT
CGGCATTCCCGCCTTGAGGGTCGACGGCAACGACTATCTCGCGGTCTATGCGGTGGCGCGCTGGGCGGCGGAGCGGGCGC
GCCTGAATCTCGGTCCGACGCTGATCGAATATGTGACCTATCGCGTCGGCGCCCATTCGACCTCCGACGATCCGAGCGCC
TATCGTCCGAAAACGGAATCGGAGGCCTGGCCGCTCGGCGACCCGGTTCTGCGCCTCAAAAAGCACCTGGTTCTGCGCGG
CGTCTGGTCCGAGGAACGGCATGCGCAGGCGGAGGCGGAAATCATGGACGAAGTCATCCAGGCACAGAAGGAAGCCGAGC
GCCACGGAACGCTCCATGCCGGCGGCAGGCCGTCGGTGCGGGACATCTTCGAAGGCGTCTATGCCGAGATGCCGCCCCAT
ATCCGCCGCCAGCGGCAGAAAGCGGGGTACTGA

Upstream 100 bases:

>100_bases
GGAGGAACCCGGGCCAGGGAAACTGCCGGGCGGCATGGCACCGTTCTCCCGGGGCAGCGAGTTGCGCAAGGCGCAATCCG
GAAGGGAGGCCGGAAGCCGC

Downstream 100 bases:

>100_bases
CATGGCCAGAATGACGATGATCGAAGCGGTGCGCAGCGCCATGGACGTGTCGATGGCGCGCGACGAGGACGTCGTCGTCT
TCGGCGAGGACGTCGGCTAT

Product: 3-methyl-2-oxobutanoate dehydrogenase

Products: NA

Alternate protein names: Branched-chain alpha-keto acid dehydrogenase E1 component alpha chain; BCKDH E1-alpha [H]

Number of amino acids: Translated: 410; Mature: 410

Protein sequence:

>410_residues
MDEFSRLSLHVPEPAVRPGDQPDFSNVKIPKAGSVPRTEVDVDPEEIRDLAYSIIRVLNREGEAVGPWAGLLSDEELLTG
LRHMMLLRAFDARMLMAQRQGKTSFYMQHLGEEAVSCAFRKALRKGDMNFPTYRQAGLLIADDYPMVEMMNQIFSNELDP
CHGRQLPVMYTSKAHGFFTISGNLATQYVQAVGWAMASAIKNDTRIAAGWIGDGSTAESDFHSALVFASTYKAPVILNIV
NNQWAISTFQGIARGGSGTFAARGLGFGIPALRVDGNDYLAVYAVARWAAERARLNLGPTLIEYVTYRVGAHSTSDDPSA
YRPKTESEAWPLGDPVLRLKKHLVLRGVWSEERHAQAEAEIMDEVIQAQKEAERHGTLHAGGRPSVRDIFEGVYAEMPPH
IRRQRQKAGY

Sequences:

>Translated_410_residues
MDEFSRLSLHVPEPAVRPGDQPDFSNVKIPKAGSVPRTEVDVDPEEIRDLAYSIIRVLNREGEAVGPWAGLLSDEELLTG
LRHMMLLRAFDARMLMAQRQGKTSFYMQHLGEEAVSCAFRKALRKGDMNFPTYRQAGLLIADDYPMVEMMNQIFSNELDP
CHGRQLPVMYTSKAHGFFTISGNLATQYVQAVGWAMASAIKNDTRIAAGWIGDGSTAESDFHSALVFASTYKAPVILNIV
NNQWAISTFQGIARGGSGTFAARGLGFGIPALRVDGNDYLAVYAVARWAAERARLNLGPTLIEYVTYRVGAHSTSDDPSA
YRPKTESEAWPLGDPVLRLKKHLVLRGVWSEERHAQAEAEIMDEVIQAQKEAERHGTLHAGGRPSVRDIFEGVYAEMPPH
IRRQRQKAGY
>Mature_410_residues
MDEFSRLSLHVPEPAVRPGDQPDFSNVKIPKAGSVPRTEVDVDPEEIRDLAYSIIRVLNREGEAVGPWAGLLSDEELLTG
LRHMMLLRAFDARMLMAQRQGKTSFYMQHLGEEAVSCAFRKALRKGDMNFPTYRQAGLLIADDYPMVEMMNQIFSNELDP
CHGRQLPVMYTSKAHGFFTISGNLATQYVQAVGWAMASAIKNDTRIAAGWIGDGSTAESDFHSALVFASTYKAPVILNIV
NNQWAISTFQGIARGGSGTFAARGLGFGIPALRVDGNDYLAVYAVARWAAERARLNLGPTLIEYVTYRVGAHSTSDDPSA
YRPKTESEAWPLGDPVLRLKKHLVLRGVWSEERHAQAEAEIMDEVIQAQKEAERHGTLHAGGRPSVRDIFEGVYAEMPPH
IRRQRQKAGY

Specific function: The branched-chain alpha-keto dehydrogenase complex catalyzes the overall conversion of alpha-keto acids to acyl-CoA and CO(2). It contains multiple copies of three enzymatic components:branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltran

COG id: COG1071

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dehydrogenase (E1) component, eukaryotic type, alpha subunit

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the BCKDHA family [H]

Homologues:

Organism=Homo sapiens, GI11386135, Length=355, Percent_Identity=40.2816901408451, Blast_Score=237, Evalue=1e-62,
Organism=Homo sapiens, GI258645172, Length=355, Percent_Identity=40, Blast_Score=235, Evalue=5e-62,
Organism=Homo sapiens, GI291084742, Length=337, Percent_Identity=25.8160237388724, Blast_Score=94, Evalue=3e-19,
Organism=Homo sapiens, GI4505685, Length=337, Percent_Identity=25.8160237388724, Blast_Score=93, Evalue=3e-19,
Organism=Homo sapiens, GI291084744, Length=227, Percent_Identity=29.0748898678414, Blast_Score=91, Evalue=3e-18,
Organism=Homo sapiens, GI4885543, Length=312, Percent_Identity=26.9230769230769, Blast_Score=88, Evalue=1e-17,
Organism=Homo sapiens, GI291084757, Length=184, Percent_Identity=30.9782608695652, Blast_Score=78, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI86563357, Length=360, Percent_Identity=39.7222222222222, Blast_Score=235, Evalue=4e-62,
Organism=Caenorhabditis elegans, GI86563355, Length=360, Percent_Identity=39.7222222222222, Blast_Score=235, Evalue=4e-62,
Organism=Caenorhabditis elegans, GI17536047, Length=310, Percent_Identity=25.8064516129032, Blast_Score=97, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI32564172, Length=310, Percent_Identity=25.8064516129032, Blast_Score=97, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6321026, Length=334, Percent_Identity=26.0479041916168, Blast_Score=97, Evalue=7e-21,
Organism=Drosophila melanogaster, GI21355903, Length=392, Percent_Identity=35.969387755102, Blast_Score=216, Evalue=2e-56,
Organism=Drosophila melanogaster, GI24639748, Length=305, Percent_Identity=26.2295081967213, Blast_Score=90, Evalue=4e-18,
Organism=Drosophila melanogaster, GI24639744, Length=301, Percent_Identity=23.5880398671096, Blast_Score=68, Evalue=1e-11,
Organism=Drosophila melanogaster, GI28571106, Length=301, Percent_Identity=23.5880398671096, Blast_Score=68, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24639740, Length=301, Percent_Identity=23.5880398671096, Blast_Score=68, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24639746, Length=301, Percent_Identity=23.5880398671096, Blast_Score=68, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001017
- InterPro:   IPR022593 [H]

Pfam domain/function: PF00676 E1_dh; PF12573 OxoDH_E1alpha_N [H]

EC number: =1.2.4.4 [H]

Molecular weight: Translated: 45504; Mature: 45504

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDEFSRLSLHVPEPAVRPGDQPDFSNVKIPKAGSVPRTEVDVDPEEIRDLAYSIIRVLNR
CCCCCCCEEECCCCCCCCCCCCCCCCEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCC
EGEAVGPWAGLLSDEELLTGLRHMMLLRAFDARMLMAQRQGKTSFYMQHLGEEAVSCAFR
CCCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
KALRKGDMNFPTYRQAGLLIADDYPMVEMMNQIFSNELDPCHGRQLPVMYTSKAHGFFTI
HHHHHCCCCCCCHHHCCEEEECCCCHHHHHHHHHCCCCCCCCCCCCCEEEECCCCEEEEE
SGNLATQYVQAVGWAMASAIKNDTRIAAGWIGDGSTAESDFHSALVFASTYKAPVILNIV
ECCHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHCCCCCCEEEEEE
NNQWAISTFQGIARGGSGTFAARGLGFGIPALRVDGNDYLAVYAVARWAAERARLNLGPT
CCCCHHHHHHHHHCCCCCCEEECCCCCCCCEEEECCCCEEHHHHHHHHHHHHHHCCCCHH
LIEYVTYRVGAHSTSDDPSAYRPKTESEAWPLGDPVLRLKKHLVLRGVWSEERHAQAEAE
HHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHH
IMDEVIQAQKEAERHGTLHAGGRPSVRDIFEGVYAEMPPHIRRQRQKAGY
HHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHCCC
>Mature Secondary Structure
MDEFSRLSLHVPEPAVRPGDQPDFSNVKIPKAGSVPRTEVDVDPEEIRDLAYSIIRVLNR
CCCCCCCEEECCCCCCCCCCCCCCCCEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCC
EGEAVGPWAGLLSDEELLTGLRHMMLLRAFDARMLMAQRQGKTSFYMQHLGEEAVSCAFR
CCCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
KALRKGDMNFPTYRQAGLLIADDYPMVEMMNQIFSNELDPCHGRQLPVMYTSKAHGFFTI
HHHHHCCCCCCCHHHCCEEEECCCCHHHHHHHHHCCCCCCCCCCCCCEEEECCCCEEEEE
SGNLATQYVQAVGWAMASAIKNDTRIAAGWIGDGSTAESDFHSALVFASTYKAPVILNIV
ECCHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHCCCCCCEEEEEE
NNQWAISTFQGIARGGSGTFAARGLGFGIPALRVDGNDYLAVYAVARWAAERARLNLGPT
CCCCHHHHHHHHHCCCCCCEEECCCCCCCCEEEECCCCEEHHHHHHHHHHHHHHCCCCHH
LIEYVTYRVGAHSTSDDPSAYRPKTESEAWPLGDPVLRLKKHLVLRGVWSEERHAQAEAE
HHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHH
IMDEVIQAQKEAERHGTLHAGGRPSVRDIFEGVYAEMPPHIRRQRQKAGY
HHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]