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The map label for this gene is rihB [H]

Identifier: 150398000

GI number: 150398000

Start: 2924595

End: 2925548

Strand: Direct

Name: rihB [H]

Synonym: Smed_2802

Alternate gene names: 150398000

Gene position: 2924595-2925548 (Clockwise)

Preceding gene: 150397998

Following gene: 150398001

Centisome position: 77.33

GC content: 63.42

Gene sequence:

>954_bases
ATGCACAAAGTTATCTTCGATACGGATCCGGGCGTCGACGACGCGATGGCCCTTCTCTTTCTGCACCGTCACCCGGCAAT
CGACCTCGTCGGCATCACCACCGTCTTCGGCAATGCGTCGATCGAAACGACGACACGCAACGCGCTGTTCCTGAAGCAGG
CATGGAATATCCCCGCGCCGGTCGCCAAGGGGAGCGGCGAGACGCTTGATCCCGACCGGCCGCATGTCGGATGGCCGACA
GGCATTCACGGTGACGACGGACTCGGCAATATCGGCGTGCCGGCGGAAATAGACCTGCCGCTCGATCCGCGATGCGCCCA
TCGCTTCATCATCGACACCGTACGCGCCAACCCGGGCGACGTGACGCTCGTCGCCGTCGGGCGGATGACGAACCTGGCGC
GCGCGCTGCGCGCGGACCCGGAGATCGCCAGGCTCGTCAGGTCGGTCGTGATCATGGGCGGTGCTTTCGATGTCACGGGC
AACATCACTCCTGCCGCCGAAGCCAATATCCATGGCGACCCGGAAGCGGCCGATGCAGTGATGACGGCCCCGTGGCCGGT
TGCCGTCATCGGTCTGGACGTGACGACGAAGACGGTGATGAGCCGCGCAATGCTCTCCGACATCGCCGAGCGTGGCGGCA
GGCCTGCGAGACTTCTCTCCGATATCTCGCAGTTCTACATCGACTTCTATGAGCATCACGTGGATGACGGCATGATCGTC
CATGACAGCTGCGCCTGTGTCTATGTCGTCGCGCCCGAGCTCTTTCAGACCCGCGGCGGCGCGATCCGGGTCATTTGCGG
CGGCATAGCCGACGGCCAGACCCTTCAAAAACCGGATGGACGACTTTTTCCGCCGAACGCCTGGGACGACTTCCCAAGCC
AGCAGACCTGCGTCGGCATCGATGCCGATGCAGTGTTGAAGCTCATCGGCGACACGCTTGCGAACGGCAGCTGA

Upstream 100 bases:

>100_bases
GCACGCTGCGCCGGCGATTGACTTTCGCGCGCGGCTTGTCCATTGCGCCAAGAACGAAATCGCTCTCGGCGGCAGCCGCC
AGAACCAGGGGAGAGGCGAT

Downstream 100 bases:

>100_bases
GGAACTGCGTTCGCGGCAATTCAACGGCCCGTCGATGGAACCGGTGACCGGCACGATACCCCCGCTCCCTTACCCGATGG
CCAAATCGCCACGGCGCAGC

Product: inosine/uridine-preferring nucleoside hydrolase

Products: NA

Alternate protein names: Cytidine/uridine-specific hydrolase [H]

Number of amino acids: Translated: 317; Mature: 317

Protein sequence:

>317_residues
MHKVIFDTDPGVDDAMALLFLHRHPAIDLVGITTVFGNASIETTTRNALFLKQAWNIPAPVAKGSGETLDPDRPHVGWPT
GIHGDDGLGNIGVPAEIDLPLDPRCAHRFIIDTVRANPGDVTLVAVGRMTNLARALRADPEIARLVRSVVIMGGAFDVTG
NITPAAEANIHGDPEAADAVMTAPWPVAVIGLDVTTKTVMSRAMLSDIAERGGRPARLLSDISQFYIDFYEHHVDDGMIV
HDSCACVYVVAPELFQTRGGAIRVICGGIADGQTLQKPDGRLFPPNAWDDFPSQQTCVGIDADAVLKLIGDTLANGS

Sequences:

>Translated_317_residues
MHKVIFDTDPGVDDAMALLFLHRHPAIDLVGITTVFGNASIETTTRNALFLKQAWNIPAPVAKGSGETLDPDRPHVGWPT
GIHGDDGLGNIGVPAEIDLPLDPRCAHRFIIDTVRANPGDVTLVAVGRMTNLARALRADPEIARLVRSVVIMGGAFDVTG
NITPAAEANIHGDPEAADAVMTAPWPVAVIGLDVTTKTVMSRAMLSDIAERGGRPARLLSDISQFYIDFYEHHVDDGMIV
HDSCACVYVVAPELFQTRGGAIRVICGGIADGQTLQKPDGRLFPPNAWDDFPSQQTCVGIDADAVLKLIGDTLANGS
>Mature_317_residues
MHKVIFDTDPGVDDAMALLFLHRHPAIDLVGITTVFGNASIETTTRNALFLKQAWNIPAPVAKGSGETLDPDRPHVGWPT
GIHGDDGLGNIGVPAEIDLPLDPRCAHRFIIDTVRANPGDVTLVAVGRMTNLARALRADPEIARLVRSVVIMGGAFDVTG
NITPAAEANIHGDPEAADAVMTAPWPVAVIGLDVTTKTVMSRAMLSDIAERGGRPARLLSDISQFYIDFYEHHVDDGMIV
HDSCACVYVVAPELFQTRGGAIRVICGGIADGQTLQKPDGRLFPPNAWDDFPSQQTCVGIDADAVLKLIGDTLANGS

Specific function: Hydrolyzes cytidine or uridine to ribose and cytosine or uracil, respectively. Has a clear preference for cytidine over uridine. Strictly specific for ribonucleosides [H]

COG id: COG1957

COG function: function code F; Inosine-uridine nucleoside N-ribohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IUNH family. RihB subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788486, Length=288, Percent_Identity=37.5, Blast_Score=162, Evalue=2e-41,
Organism=Escherichia coli, GI1786213, Length=318, Percent_Identity=33.6477987421384, Blast_Score=129, Evalue=3e-31,
Organism=Escherichia coli, GI1786871, Length=261, Percent_Identity=33.7164750957854, Blast_Score=121, Evalue=6e-29,
Organism=Caenorhabditis elegans, GI32565474, Length=248, Percent_Identity=30.241935483871, Blast_Score=83, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17565698, Length=190, Percent_Identity=31.5789473684211, Blast_Score=73, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6320608, Length=256, Percent_Identity=31.640625, Blast_Score=98, Evalue=2e-21,
Organism=Drosophila melanogaster, GI24641839, Length=200, Percent_Identity=33.5, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24641837, Length=186, Percent_Identity=34.4086021505376, Blast_Score=67, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015910
- InterPro:   IPR023186
- InterPro:   IPR001910
- InterPro:   IPR022977 [H]

Pfam domain/function: PF01156 IU_nuc_hydro [H]

EC number: =3.2.2.8 [H]

Molecular weight: Translated: 33765; Mature: 33765

Theoretical pI: Translated: 4.70; Mature: 4.70

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHKVIFDTDPGVDDAMALLFLHRHPAIDLVGITTVFGNASIETTTRNALFLKQAWNIPAP
CCEEEECCCCCHHHHHHHHHHHCCCCEEEEEEEEEECCCEEEECCCCEEEEEHHCCCCCC
VAKGSGETLDPDRPHVGWPTGIHGDDGLGNIGVPAEIDLPLDPRCAHRFIIDTVRANPGD
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEECCCCCCHHHHHHHHHHCCCCCCC
VTLVAVGRMTNLARALRADPEIARLVRSVVIMGGAFDVTGNITPAAEANIHGDPEAADAV
EEEEEECHHHHHHHHHHCCHHHHHHHHHHHHCCCCEEECCCCCCCCCCCCCCCCHHHCEE
MTAPWPVAVIGLDVTTKTVMSRAMLSDIAERGGRPARLLSDISQFYIDFYEHHVDDGMIV
EECCCCEEEEEECHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCEEE
HDSCACVYVVAPELFQTRGGAIRVICGGIADGQTLQKPDGRLFPPNAWDDFPSQQTCVGI
ECCCCEEEEECHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEC
DADAVLKLIGDTLANGS
CHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MHKVIFDTDPGVDDAMALLFLHRHPAIDLVGITTVFGNASIETTTRNALFLKQAWNIPAP
CCEEEECCCCCHHHHHHHHHHHCCCCEEEEEEEEEECCCEEEECCCCEEEEEHHCCCCCC
VAKGSGETLDPDRPHVGWPTGIHGDDGLGNIGVPAEIDLPLDPRCAHRFIIDTVRANPGD
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEECCCCCCHHHHHHHHHHCCCCCCC
VTLVAVGRMTNLARALRADPEIARLVRSVVIMGGAFDVTGNITPAAEANIHGDPEAADAV
EEEEEECHHHHHHHHHHCCHHHHHHHHHHHHCCCCEEECCCCCCCCCCCCCCCCHHHCEE
MTAPWPVAVIGLDVTTKTVMSRAMLSDIAERGGRPARLLSDISQFYIDFYEHHVDDGMIV
EECCCCEEEEEECHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCEEE
HDSCACVYVVAPELFQTRGGAIRVICGGIADGQTLQKPDGRLFPPNAWDDFPSQQTCVGI
ECCCCEEEEECHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEC
DADAVLKLIGDTLANGS
CHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA