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The map label for this gene is nodN [H]

Identifier: 150397943

GI number: 150397943

Start: 2863361

End: 2863849

Strand: Direct

Name: nodN [H]

Synonym: Smed_2745

Alternate gene names: 150397943

Gene position: 2863361-2863849 (Clockwise)

Preceding gene: 150397942

Following gene: 150397944

Centisome position: 75.71

GC content: 58.49

Gene sequence:

>489_bases
ATGCCGCAGGATATTTCACTTTCCGACATCAAGGAGCTGATCGGCAAGGAGCTCGGCGTCTCCGACTGGATTACCGTGAC
GCAAAAAACGATCGACCGGTTCGCCGAGGCGACGGGTGACTTCCAGTACATTCACACCGATCCGGTGCGCGCTGCCGCGG
AAACGCCTTTCGGTGGTACGATCGCACATGGCTTCCTGTCGCTGTCGCTGCTTTCGGCGATGAACTACAATTGTCTGCCT
AAGATCCGCGAGCAGACGATGGGCATCAACTACGGCTTCGACAAGGTGCGCTTCATGGCGCCAGTCAAGAGCGGTGCGCG
CGTACGCGGCCGCTTTACGATGGCGGACGCACGTTTCCGAGGGGCCGGAATGCTGATGATTACCTACGATGTTACCGTCG
AAATCGAAGGCGAAAGGAAACCGGCGCTGACCGCGACCTGGCAGACGATCATCCAGTTCGATCCGAAAGACCGGCCAGCA
GATGTCTGA

Upstream 100 bases:

>100_bases
TCACCTTTCTCGTCGAGTTTTTTTAACGACTTACGACCACTTGCTAGACCTTGCCCGGCCGGCCTATAGATCGGCAGGGG
AGGCTATTTAGGACAGCCAT

Downstream 100 bases:

>100_bases
CGCGACGCCAGCCGGGACGCGCGTTCGCGATGCTTTCCGAGATCAGGCAAGATCCTGCGACGAACTGGGATCGCCATTTA
CCGCCCGGCTCTGCCGTCTC

Product: dehydratase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 162; Mature: 161

Protein sequence:

>162_residues
MPQDISLSDIKELIGKELGVSDWITVTQKTIDRFAEATGDFQYIHTDPVRAAAETPFGGTIAHGFLSLSLLSAMNYNCLP
KIREQTMGINYGFDKVRFMAPVKSGARVRGRFTMADARFRGAGMLMITYDVTVEIEGERKPALTATWQTIIQFDPKDRPA
DV

Sequences:

>Translated_162_residues
MPQDISLSDIKELIGKELGVSDWITVTQKTIDRFAEATGDFQYIHTDPVRAAAETPFGGTIAHGFLSLSLLSAMNYNCLP
KIREQTMGINYGFDKVRFMAPVKSGARVRGRFTMADARFRGAGMLMITYDVTVEIEGERKPALTATWQTIIQFDPKDRPA
DV
>Mature_161_residues
PQDISLSDIKELIGKELGVSDWITVTQKTIDRFAEATGDFQYIHTDPVRAAAETPFGGTIAHGFLSLSLLSAMNYNCLPK
IREQTMGINYGFDKVRFMAPVKSGARVRGRFTMADARFRGAGMLMITYDVTVEIEGERKPALTATWQTIIQFDPKDRPAD
V

Specific function: Involved in the production of the root hair deformation (HAD) factor specifically on medicago [H]

COG id: COG2030

COG function: function code I; Acyl dehydratase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: To the R.meliloti counterpart [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002539 [H]

Pfam domain/function: PF01575 MaoC_dehydratas [H]

EC number: NA

Molecular weight: Translated: 18037; Mature: 17906

Theoretical pI: Translated: 6.53; Mature: 6.53

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPQDISLSDIKELIGKELGVSDWITVTQKTIDRFAEATGDFQYIHTDPVRAAAETPFGGT
CCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCEEEEECCCCCHHCCCCCCHH
IAHGFLSLSLLSAMNYNCLPKIREQTMGINYGFDKVRFMAPVKSGARVRGRFTMADARFR
HHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCCCCEEEEECCCCCCEEEEEEEEECCEEC
GAGMLMITYDVTVEIEGERKPALTATWQTIIQFDPKDRPADV
CCCEEEEEEEEEEEECCCCCCCHHHEEEEHEEECCCCCCCCC
>Mature Secondary Structure 
PQDISLSDIKELIGKELGVSDWITVTQKTIDRFAEATGDFQYIHTDPVRAAAETPFGGT
CCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCEEEEECCCCCHHCCCCCCHH
IAHGFLSLSLLSAMNYNCLPKIREQTMGINYGFDKVRFMAPVKSGARVRGRFTMADARFR
HHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCCCCEEEEECCCCCCEEEEEEEEECCEEC
GAGMLMITYDVTVEIEGERKPALTATWQTIIQFDPKDRPADV
CCCEEEEEEEEEEEECCCCCCCHHHEEEEHEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3132583 [H]