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The map label for this gene is glgX [H]

Identifier: 150397942

GI number: 150397942

Start: 2861322

End: 2863286

Strand: Direct

Name: glgX [H]

Synonym: Smed_2744

Alternate gene names: 150397942

Gene position: 2861322-2863286 (Clockwise)

Preceding gene: 150397941

Following gene: 150397943

Centisome position: 75.66

GC content: 63.82

Gene sequence:

>1965_bases
ATGCCGACGACCGGCTTTCCACCACTCGGCGTGACACGGGCAGCCGACGGAACACGCTTTACGGTCTGGTCGCATAATGC
AGCCCGCATCGATCTCTGCCTGTTCGACGGAGCCGGGATGGCAGAGCTCAGCCGCCTGCCGATGCTGCGCGATGGCGACG
TCCACAGCATCATTCTGCCCGACATAGCGCCCGGCACCCGCTACGGCCTGCGCGCCGACGGCGTGTACTCCCCGGATCAT
GGGCTTTGGTTCGATCCGTCGAAGCTTCTGGTCGACCCTTACGCGGTCGAGCTCGACGGGCCTTTTCGTCACGATCCGCG
GCTGACGGCCTTCGGCGAAGACACGGCCGACCTCGTCCCGAAAGCAGTCGTCACGGAAGCGAAGACGGTAAAACCCGGGC
CTCCACTCTTTTCATCGGGCGCGCTGATCTACGAAGTTGCGGTCAAGCCGTTTACGATCCTTCACCCGGGCGTACCGGAA
AAGAAACGCGGCACTGTGGCGGCTCTGGCCGAACCCGCCATCATCGAACATCTCACGCGCCTCGGGGTTTCCGCGGTCGA
ACTCATGCCGGTGGTTGCCTGGATCGACGAGCGGCATCTGCCTCCGCTCGGCCTGCGCAACGGTTGGGGCTACAATCCGA
TTGCGCCGATGGCTCTCGATCCGCGCCTGGTGCCGGGCGGCATCAGGGAACTGCGCGAAACCGTAGATGCCCTGCATCAG
GCCGGCATCGGCGTCATCCTTGATCTCGTCTTCAACCATTCCGGCGAGAGCGATCGTTTCGGCGCCACGCTTTCGATGCG
CGGACTGGACAACCTGACCTATTATCGCCATGCCACCGGCCGGCCGGGCGAACTCATCAACGACACGGGCTGCGGCAACA
CGATCGCCTGCGACCACCCGGTCGTCCAGCGACTGATCGTCGACAGTTTGCGGCATTTCGTACTTGCCGCCGGCATTGAC
GGTTTCCGCTTCGACCTCGCCTCGATTCTCGGCCGCGACATGGGTGGCTTCCGCCGCGATGCCGCGCTTTTCTCGGCGAT
GGCAGCCGACCCGGTCCTTTGCGACCGCGTGTTCATCGCCGAGCCCTGGGACACCGGGCCCGGCGGCTACCAGCTCGGCA
ATTTCCCGCCTCCCTTTCTGGAATGGAACGATCGGGCACGCGACGACATCCGCCGCTACTGGCGCGGCGATCGTCACACG
GTCGGTGCGTTTGCGACAGCGCTCGCCGGCTCCTCCGCCAGCTTTTCTCGATGGGGCGAGACGGACACCCGCAGCCTCAA
CTTCATCGCCGCCCACGATGGCTTCACGTTGATGGATCTCGTCTCCTACGCCCGCAAGCACAATGAGGCGAACGGCGAGG
GTAATCGGGATGGCCACGACGAGAACTTCTCGTGGAACAACGGTGCCGAAGGTCCGGTAACGGATCCGGAGATCGCCTCT
TTCCGGCAGCGCGATGTCATGGCGCTCCTCAGCACTCTCTTCGCCTCGCGCGGTGCAATCATGATGAGAGCCGGCGATGA
AGGCGGCCACAGTCAGGGCGGCAACAACAACGCCTATGCGCAGGACAATGCCGTGACCTGGCTCGACTGGAGCAAACCGG
ACGGCACACTCGTCGAGCACTCTGCACGGCTTTCCGCCATGCGCCGGCGTTTCGGCGTCTTTGCCGAGACCGCCTTCTTC
ACCGGCATCGACGATGTCGCCTGGCTATGTCTCGATGGAGAGCCCCTAACCGTCGAGCAATGGGAGCACCCCGCCACCGA
CAATCTCGTCATGATGCTTGCGACCGAAGATCGCCACCAGAAAAAAGCCACCCGGCTTGCCGTGGTCGTCAACCGCAGCC
ACGCGCCACACCCGATCCGGCTTCCGGCGAACCTCAAGAACGAATGGCGCGACGCTCTCTCGGATGGACCGGCACCGGCA
AGCGTACCAGCCCGTTCGGTCACCTTTCTCGTCGAGTTTTTTTAA

Upstream 100 bases:

>100_bases
GCCGCCCTCTGTGCGCCCTTTCCCCCTCCGCCCTGCCGGGCAGAGGGGGATCAACACCGGCACTGACTTGAGCTGCAATA
TCATGAGGGGGCCCGCACTC

Downstream 100 bases:

>100_bases
CGACTTACGACCACTTGCTAGACCTTGCCCGGCCGGCCTATAGATCGGCAGGGGAGGCTATTTAGGACAGCCATATGCCG
CAGGATATTTCACTTTCCGA

Product: glycogen debranching protein GlgX

Products: NA

Alternate protein names: Glycogen operon protein GlgX [H]

Number of amino acids: Translated: 654; Mature: 653

Protein sequence:

>654_residues
MPTTGFPPLGVTRAADGTRFTVWSHNAARIDLCLFDGAGMAELSRLPMLRDGDVHSIILPDIAPGTRYGLRADGVYSPDH
GLWFDPSKLLVDPYAVELDGPFRHDPRLTAFGEDTADLVPKAVVTEAKTVKPGPPLFSSGALIYEVAVKPFTILHPGVPE
KKRGTVAALAEPAIIEHLTRLGVSAVELMPVVAWIDERHLPPLGLRNGWGYNPIAPMALDPRLVPGGIRELRETVDALHQ
AGIGVILDLVFNHSGESDRFGATLSMRGLDNLTYYRHATGRPGELINDTGCGNTIACDHPVVQRLIVDSLRHFVLAAGID
GFRFDLASILGRDMGGFRRDAALFSAMAADPVLCDRVFIAEPWDTGPGGYQLGNFPPPFLEWNDRARDDIRRYWRGDRHT
VGAFATALAGSSASFSRWGETDTRSLNFIAAHDGFTLMDLVSYARKHNEANGEGNRDGHDENFSWNNGAEGPVTDPEIAS
FRQRDVMALLSTLFASRGAIMMRAGDEGGHSQGGNNNAYAQDNAVTWLDWSKPDGTLVEHSARLSAMRRRFGVFAETAFF
TGIDDVAWLCLDGEPLTVEQWEHPATDNLVMMLATEDRHQKKATRLAVVVNRSHAPHPIRLPANLKNEWRDALSDGPAPA
SVPARSVTFLVEFF

Sequences:

>Translated_654_residues
MPTTGFPPLGVTRAADGTRFTVWSHNAARIDLCLFDGAGMAELSRLPMLRDGDVHSIILPDIAPGTRYGLRADGVYSPDH
GLWFDPSKLLVDPYAVELDGPFRHDPRLTAFGEDTADLVPKAVVTEAKTVKPGPPLFSSGALIYEVAVKPFTILHPGVPE
KKRGTVAALAEPAIIEHLTRLGVSAVELMPVVAWIDERHLPPLGLRNGWGYNPIAPMALDPRLVPGGIRELRETVDALHQ
AGIGVILDLVFNHSGESDRFGATLSMRGLDNLTYYRHATGRPGELINDTGCGNTIACDHPVVQRLIVDSLRHFVLAAGID
GFRFDLASILGRDMGGFRRDAALFSAMAADPVLCDRVFIAEPWDTGPGGYQLGNFPPPFLEWNDRARDDIRRYWRGDRHT
VGAFATALAGSSASFSRWGETDTRSLNFIAAHDGFTLMDLVSYARKHNEANGEGNRDGHDENFSWNNGAEGPVTDPEIAS
FRQRDVMALLSTLFASRGAIMMRAGDEGGHSQGGNNNAYAQDNAVTWLDWSKPDGTLVEHSARLSAMRRRFGVFAETAFF
TGIDDVAWLCLDGEPLTVEQWEHPATDNLVMMLATEDRHQKKATRLAVVVNRSHAPHPIRLPANLKNEWRDALSDGPAPA
SVPARSVTFLVEFF
>Mature_653_residues
PTTGFPPLGVTRAADGTRFTVWSHNAARIDLCLFDGAGMAELSRLPMLRDGDVHSIILPDIAPGTRYGLRADGVYSPDHG
LWFDPSKLLVDPYAVELDGPFRHDPRLTAFGEDTADLVPKAVVTEAKTVKPGPPLFSSGALIYEVAVKPFTILHPGVPEK
KRGTVAALAEPAIIEHLTRLGVSAVELMPVVAWIDERHLPPLGLRNGWGYNPIAPMALDPRLVPGGIRELRETVDALHQA
GIGVILDLVFNHSGESDRFGATLSMRGLDNLTYYRHATGRPGELINDTGCGNTIACDHPVVQRLIVDSLRHFVLAAGIDG
FRFDLASILGRDMGGFRRDAALFSAMAADPVLCDRVFIAEPWDTGPGGYQLGNFPPPFLEWNDRARDDIRRYWRGDRHTV
GAFATALAGSSASFSRWGETDTRSLNFIAAHDGFTLMDLVSYARKHNEANGEGNRDGHDENFSWNNGAEGPVTDPEIASF
RQRDVMALLSTLFASRGAIMMRAGDEGGHSQGGNNNAYAQDNAVTWLDWSKPDGTLVEHSARLSAMRRRFGVFAETAFFT
GIDDVAWLCLDGEPLTVEQWEHPATDNLVMMLATEDRHQKKATRLAVVVNRSHAPHPIRLPANLKNEWRDALSDGPAPAS
VPARSVTFLVEFF

Specific function: Hydrolyzes the alpha-1,6-glucosidic linkages in glycogen which has first been partially depolymerized by phosphorylase. Shows only very little activity with native glycogen [H]

COG id: COG1523

COG function: function code G; Type II secretory pathway, pullulanase PulA and related glycosidases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family [H]

Homologues:

Organism=Escherichia coli, GI2367229, Length=635, Percent_Identity=42.3622047244094, Blast_Score=467, Evalue=1e-132,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013780
- InterPro:   IPR006047
- InterPro:   IPR004193
- InterPro:   IPR017853
- InterPro:   IPR013781
- InterPro:   IPR022844
- InterPro:   IPR011837
- InterPro:   IPR013783
- InterPro:   IPR014756 [H]

Pfam domain/function: PF00128 Alpha-amylase; PF02922 CBM_48 [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 71528; Mature: 71397

Theoretical pI: Translated: 5.76; Mature: 5.76

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPTTGFPPLGVTRAADGTRFTVWSHNAARIDLCLFDGAGMAELSRLPMLRDGDVHSIILP
CCCCCCCCCCCEECCCCCEEEEEECCCEEEEEEEECCCCHHHHHHCCCCCCCCCCEEECC
DIAPGTRYGLRADGVYSPDHGLWFDPSKLLVDPYAVELDGPFRHDPRLTAFGEDTADLVP
CCCCCCCCCEEECCCCCCCCCCEECCHHHEECCEEEEECCCCCCCCCEEECCCCHHHHCC
KAVVTEAKTVKPGPPLFSSGALIYEVAVKPFTILHPGVPEKKRGTVAALAEPAIIEHLTR
HHHHHCCCCCCCCCCCCCCCCEEEEEECCCEEEECCCCCCCCCCCCHHHCCCHHHHHHHH
LGVSAVELMPVVAWIDERHLPPLGLRNGWGYNPIAPMALDPRLVPGGIRELRETVDALHQ
HCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
AGIGVILDLVFNHSGESDRFGATLSMRGLDNLTYYRHATGRPGELINDTGCGNTIACDHP
CCHHHHHHHHHCCCCCCCCCCCEEEECCCCCCEEEEECCCCCCCCCCCCCCCCCEECCHH
VVQRLIVDSLRHFVLAAGIDGFRFDLASILGRDMGGFRRDAALFSAMAADPVLCDRVFIA
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCHHCCEEEEE
EPWDTGPGGYQLGNFPPPFLEWNDRARDDIRRYWRGDRHTVGAFATALAGSSASFSRWGE
CCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHCCCCCCHHHCCC
TDTRSLNFIAAHDGFTLMDLVSYARKHNEANGEGNRDGHDENFSWNNGAEGPVTDPEIAS
CCCCCEEEEEECCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHH
FRQRDVMALLSTLFASRGAIMMRAGDEGGHSQGGNNNAYAQDNAVTWLDWSKPDGTLVEH
HHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCEECCCEEEEEECCCCCCCHHHH
SARLSAMRRRFGVFAETAFFTGIDDVAWLCLDGEPLTVEQWEHPATDNLVMMLATEDRHQ
HHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCHHCCCCCCCCCEEEEEECCCHHH
KKATRLAVVVNRSHAPHPIRLPANLKNEWRDALSDGPAPASVPARSVTFLVEFF
HHHEEEEEEEECCCCCCCEECCCCCHHHHHHHHCCCCCCCCCCCCEEEEEEEEC
>Mature Secondary Structure 
PTTGFPPLGVTRAADGTRFTVWSHNAARIDLCLFDGAGMAELSRLPMLRDGDVHSIILP
CCCCCCCCCCEECCCCCEEEEEECCCEEEEEEEECCCCHHHHHHCCCCCCCCCCEEECC
DIAPGTRYGLRADGVYSPDHGLWFDPSKLLVDPYAVELDGPFRHDPRLTAFGEDTADLVP
CCCCCCCCCEEECCCCCCCCCCEECCHHHEECCEEEEECCCCCCCCCEEECCCCHHHHCC
KAVVTEAKTVKPGPPLFSSGALIYEVAVKPFTILHPGVPEKKRGTVAALAEPAIIEHLTR
HHHHHCCCCCCCCCCCCCCCCEEEEEECCCEEEECCCCCCCCCCCCHHHCCCHHHHHHHH
LGVSAVELMPVVAWIDERHLPPLGLRNGWGYNPIAPMALDPRLVPGGIRELRETVDALHQ
HCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
AGIGVILDLVFNHSGESDRFGATLSMRGLDNLTYYRHATGRPGELINDTGCGNTIACDHP
CCHHHHHHHHHCCCCCCCCCCCEEEECCCCCCEEEEECCCCCCCCCCCCCCCCCEECCHH
VVQRLIVDSLRHFVLAAGIDGFRFDLASILGRDMGGFRRDAALFSAMAADPVLCDRVFIA
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCHHCCEEEEE
EPWDTGPGGYQLGNFPPPFLEWNDRARDDIRRYWRGDRHTVGAFATALAGSSASFSRWGE
CCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHCCCCCCHHHCCC
TDTRSLNFIAAHDGFTLMDLVSYARKHNEANGEGNRDGHDENFSWNNGAEGPVTDPEIAS
CCCCCEEEEEECCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHH
FRQRDVMALLSTLFASRGAIMMRAGDEGGHSQGGNNNAYAQDNAVTWLDWSKPDGTLVEH
HHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCEECCCEEEEEECCCCCCCHHHH
SARLSAMRRRFGVFAETAFFTGIDDVAWLCLDGEPLTVEQWEHPATDNLVMMLATEDRHQ
HHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCHHCCCCCCCCCEEEEEECCCHHH
KKATRLAVVVNRSHAPHPIRLPANLKNEWRDALSDGPAPASVPARSVTFLVEFF
HHHEEEEEEEECCCCCCCEECCCCCHHHHHHHHCCCCCCCCCCCCEEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Glycosylases; Glycosidases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA