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The map label for this gene is yhcK [H]

Identifier: 150397885

GI number: 150397885

Start: 2790576

End: 2791526

Strand: Reverse

Name: yhcK [H]

Synonym: Smed_2687

Alternate gene names: 150397885

Gene position: 2791526-2790576 (Counterclockwise)

Preceding gene: 150397890

Following gene: 150397881

Centisome position: 73.81

GC content: 61.2

Gene sequence:

>951_bases
GTGACTGGTGATCACCTCAAGGCCCTGCTGGGCCTGCAAGCCGCAACACCGGTGTTGATCGCGCTTTATGATCCGCAGGA
TCGGTTGCGCTACGCCAATGAGGCCTTTCGGTCCGCCTACCATGTCGGGAAGGACGAGGCGCCGACCTGGGCGGAAATCA
TGCGTCGCAACCATGCCGCGGGCAGGGGTACCGTGCTCGAGACCGACGACCTCGAAGGCTGGATCGCCGCAACGCTCCAC
AGGCGCGGCAAGGTGCCCTTTCGCACGGTCGAGACCGACCTGCATGACGGTCGCTGGCTTCTGATGACGGAGACCGTGGA
TCAGAAGGGCTGGATGCTCTGCATCGCTTCCGGCATCACCGATGTACGCGCAGGGGAGCGCAAGCTTCGCCAGGACCGCG
ACTTTGCCCTGAGAGCCTCACAGGTGGACGATCTCACCAGTCTACCCAATCGGCGTTACACAATGGAGAGACTCAAGGAG
TTCATCCAGAACTGCGCCGAAAACCCGCACATGTGGGGATGCGTGGCGATCATCGATATCGACTATTTCAAGGCGATCAA
TGATCGCTTCGGCCATTACCGGGGCGATGAAGTGCTGCTCGACTTTGCCCGCCAGATGCAGGAATTCGTCCGGCGCTCCG
ACTGCTTCGGCCGCATCGGCGGCGAAGAGTTCATGCTGATCCTGCCCGACACCACCGTCAGCGAAGCCAATCTCATCCTC
GACCGGCTGCTGGACAAGATCCGGAGCGCGAGGCCGCTGTCGAACATACCGGAGTTCTTCTACACCTGTTCGGTCGGGCT
TGCTGAATACCGCGAGGGCGACAGTGTCGGCGACATCTATTCGCGCGCGGACGCGGCGCTTTATATCGCCAAGCGCGAAG
GTCGCGATCGCGTAAGTCGCTTTACGCTGCCTGGATCAGGCGATGAGTTCAGCCCGGATCAGACCCCTTAA

Upstream 100 bases:

>100_bases
TCGTTTCGCACGGCAGCTTGCCACAAGCATTACGTGCTATAGCTTCAACTGAGCTGGCCGCTTCGCAGAGCGACCTTTAT
GTTGGTGGAGCAGACCGACC

Downstream 100 bases:

>100_bases
GGAATTGCCGACATAGAGGCGGCCGCCGGCGAGGTCGGAGGGCTCCAGCCGCTCAACCCTTGCTCGCCGCCGGCAGATGA
GCTCCGTGCGCAAAACGCCG

Product: diguanylate cyclase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 316; Mature: 315

Protein sequence:

>316_residues
MTGDHLKALLGLQAATPVLIALYDPQDRLRYANEAFRSAYHVGKDEAPTWAEIMRRNHAAGRGTVLETDDLEGWIAATLH
RRGKVPFRTVETDLHDGRWLLMTETVDQKGWMLCIASGITDVRAGERKLRQDRDFALRASQVDDLTSLPNRRYTMERLKE
FIQNCAENPHMWGCVAIIDIDYFKAINDRFGHYRGDEVLLDFARQMQEFVRRSDCFGRIGGEEFMLILPDTTVSEANLIL
DRLLDKIRSARPLSNIPEFFYTCSVGLAEYREGDSVGDIYSRADAALYIAKREGRDRVSRFTLPGSGDEFSPDQTP

Sequences:

>Translated_316_residues
MTGDHLKALLGLQAATPVLIALYDPQDRLRYANEAFRSAYHVGKDEAPTWAEIMRRNHAAGRGTVLETDDLEGWIAATLH
RRGKVPFRTVETDLHDGRWLLMTETVDQKGWMLCIASGITDVRAGERKLRQDRDFALRASQVDDLTSLPNRRYTMERLKE
FIQNCAENPHMWGCVAIIDIDYFKAINDRFGHYRGDEVLLDFARQMQEFVRRSDCFGRIGGEEFMLILPDTTVSEANLIL
DRLLDKIRSARPLSNIPEFFYTCSVGLAEYREGDSVGDIYSRADAALYIAKREGRDRVSRFTLPGSGDEFSPDQTP
>Mature_315_residues
TGDHLKALLGLQAATPVLIALYDPQDRLRYANEAFRSAYHVGKDEAPTWAEIMRRNHAAGRGTVLETDDLEGWIAATLHR
RGKVPFRTVETDLHDGRWLLMTETVDQKGWMLCIASGITDVRAGERKLRQDRDFALRASQVDDLTSLPNRRYTMERLKEF
IQNCAENPHMWGCVAIIDIDYFKAINDRFGHYRGDEVLLDFARQMQEFVRRSDCFGRIGGEEFMLILPDTTVSEANLILD
RLLDKIRSARPLSNIPEFFYTCSVGLAEYREGDSVGDIYSRADAALYIAKREGRDRVSRFTLPGSGDEFSPDQTP

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GGDEF domain [H]

Homologues:

Organism=Escherichia coli, GI87081881, Length=191, Percent_Identity=35.6020942408377, Blast_Score=100, Evalue=9e-23,
Organism=Escherichia coli, GI87082007, Length=159, Percent_Identity=37.1069182389937, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1786584, Length=159, Percent_Identity=37.1069182389937, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI1788381, Length=195, Percent_Identity=33.3333333333333, Blast_Score=89, Evalue=4e-19,
Organism=Escherichia coli, GI1787541, Length=193, Percent_Identity=31.0880829015544, Blast_Score=88, Evalue=6e-19,
Organism=Escherichia coli, GI1787262, Length=174, Percent_Identity=33.3333333333333, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI145693134, Length=158, Percent_Identity=34.8101265822785, Blast_Score=82, Evalue=5e-17,
Organism=Escherichia coli, GI87081977, Length=169, Percent_Identity=28.9940828402367, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI1787802, Length=173, Percent_Identity=32.3699421965318, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI87081974, Length=160, Percent_Identity=30, Blast_Score=76, Evalue=4e-15,
Organism=Escherichia coli, GI1788085, Length=189, Percent_Identity=28.042328042328, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI1787816, Length=158, Percent_Identity=30.379746835443, Blast_Score=68, Evalue=9e-13,
Organism=Escherichia coli, GI1788956, Length=165, Percent_Identity=30.3030303030303, Blast_Score=65, Evalue=7e-12,
Organism=Escherichia coli, GI1787056, Length=160, Percent_Identity=32.5, Blast_Score=64, Evalue=9e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160
- InterPro:   IPR011620 [H]

Pfam domain/function: PF07694 5TM-5TMR_LYT; PF00990 GGDEF [H]

EC number: NA

Molecular weight: Translated: 36015; Mature: 35884

Theoretical pI: Translated: 5.48; Mature: 5.48

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTGDHLKALLGLQAATPVLIALYDPQDRLRYANEAFRSAYHVGKDEAPTWAEIMRRNHAA
CCHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCC
GRGTVLETDDLEGWIAATLHRRGKVPFRTVETDLHDGRWLLMTETVDQKGWMLCIASGIT
CCCCEEECCCCCHHHHHHHHHCCCCCCEEECCCCCCCEEEEEEECCCCCCCEEEEECCCC
DVRAGERKLRQDRDFALRASQVDDLTSLPNRRYTMERLKEFIQNCAENPHMWGCVAIIDI
HHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCEEEEEEEEH
DYFKAINDRFGHYRGDEVLLDFARQMQEFVRRSDCFGRIGGEEFMLILPDTTVSEANLIL
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHH
DRLLDKIRSARPLSNIPEFFYTCSVGLAEYREGDSVGDIYSRADAALYIAKREGRDRVSR
HHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCEEEEEECCCHHHHHE
FTLPGSGDEFSPDQTP
ECCCCCCCCCCCCCCC
>Mature Secondary Structure 
TGDHLKALLGLQAATPVLIALYDPQDRLRYANEAFRSAYHVGKDEAPTWAEIMRRNHAA
CHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCC
GRGTVLETDDLEGWIAATLHRRGKVPFRTVETDLHDGRWLLMTETVDQKGWMLCIASGIT
CCCCEEECCCCCHHHHHHHHHCCCCCCEEECCCCCCCEEEEEEECCCCCCCEEEEECCCC
DVRAGERKLRQDRDFALRASQVDDLTSLPNRRYTMERLKEFIQNCAENPHMWGCVAIIDI
HHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCEEEEEEEEH
DYFKAINDRFGHYRGDEVLLDFARQMQEFVRRSDCFGRIGGEEFMLILPDTTVSEANLIL
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHH
DRLLDKIRSARPLSNIPEFFYTCSVGLAEYREGDSVGDIYSRADAALYIAKREGRDRVSR
HHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCEEEEEECCCHHHHHE
FTLPGSGDEFSPDQTP
ECCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8969498; 9384377 [H]