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The map label for this gene is tolB [H]

Identifier: 150397825

GI number: 150397825

Start: 2727533

End: 2728843

Strand: Reverse

Name: tolB [H]

Synonym: Smed_2627

Alternate gene names: 150397825

Gene position: 2728843-2727533 (Counterclockwise)

Preceding gene: 150397826

Following gene: 150397824

Centisome position: 72.16

GC content: 61.78

Gene sequence:

>1311_bases
ATGGAAATGCTGAGACGCAATTTTTTCCGCCTTCTGATGGTGCTGGTCGCAGGCTGCGGGCTCATTGCCTCGCCGGCAAA
GGCGCTCGTCGAGATCGACATCAACAAAGGTAACGTCGAGCCGCTGCCGATCGCGATCACGGATTTCGTGCAGGGCGAGC
TTGCGCAGAAGATATCCGACGTCATTGCCGCCGATCTGAAGCGCTCCGGGCTTTTCGCTCCCATCAACAAGGGCGCGTTC
ATCGAGAAGGTCTCCAATCCCGATGCCACTCCGCGCTTCGAGGACTGGAAGGTCATCAACGCGCAGGCGCTCGTCATTGG
TCGCGTCACAAAAGAAGGCGACGGCAGGCTGAAGGCGGAGTTCCGCCTCTGGGATACCTTCGCCGGAACGCAGATGCTGG
GTCAGCAGTTCTACACCCAGCCGGAAAACTGGCGCCGGGTCGCCCACATCATCGCCGATGCGATCTATGAAAGGATCACG
GGCGAGAAGGGCTATTTTGACACGCGCATCGTCTATGTCGCCGAAAGTGGTCCGAAAAATGCGCGCCAGCGCCAGCTGGC
CATCATGGACCAGGACGGGGCCAATTCCCGCGCGCTCACCAATTCCAATGACATCGTGTTGACGCCGCGCTTCTCGCCGA
ACCGCCAGGAAATCACCTATATGTCGTTCGAGAACCAGCAGCCACGGGTCTATCTGCTGCAGCTGGAAACGGGGCAGCGC
GAGGTGGTCGGCAACTTCCCGGGCATGACCTTCGCTCCACGCTTTTCGCCGGACGGCCAGCGGGTGATCATGAGCCTGCA
GCAGGAAGGCAACGCCAATATCTATACGATGGACCTGCGCTCGCGCACGACGACGCGGCTCACCAACACCGCGGCGATCG
ACACCTCGCCGTCCTATTCGCCGGACGGAAGCCGGGTCGTTTTCGAAAGTGATCGCGGCGGCAGGCAGCAGCTCTATGTC
ATGGGTGCCGATGGCTCGGGCCAGACGCGCATCTCCTTCGGCGACGGTTCCTATTCGACGCCGGTCTGGTCCCCGCGCGG
CGATCTCATCGCCTTCACCAAGCAGTCGGGTGGGAAGTTCTCGATCGGTGTCATGAAACCGGACGGCTCGGGTGAGCGTA
TCCTCACGACAGGCTTCCATAATGAAGGTCCCACCTGGGCGCCGAACGGCCGCGTGCTGATGTTCTTCCGCCAGAACGCC
GGCGCAGGCGGCCCACAGCTCTATTCGATCGACCTGACGGGCTATAACGAGCAGCTTGTCCCGACCCAGGGCTTCGCCTC
GGACCCGGCCTGGTCGCCGCTCATGGAGTAG

Upstream 100 bases:

>100_bases
GGTCGTCGTCAACTTTGACCCGAGCTCGATGCTCTAGACAAGGCGAGCGGGATGCGGCGCGGTCCCATCCTGCTCGGGAA
ACGAAAGGCTGAAAGGCTTC

Downstream 100 bases:

>100_bases
GCCGGCGCTGCAGCGCCGTGCGTCTTATCAGAGGCACAAAGGTCGCTGTAGCACTTTGATCTGCTGCAATTTGTGTCCTT
CAATCGGATCTGATCAAAGG

Product: translocation protein TolB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 436; Mature: 436

Protein sequence:

>436_residues
MEMLRRNFFRLLMVLVAGCGLIASPAKALVEIDINKGNVEPLPIAITDFVQGELAQKISDVIAADLKRSGLFAPINKGAF
IEKVSNPDATPRFEDWKVINAQALVIGRVTKEGDGRLKAEFRLWDTFAGTQMLGQQFYTQPENWRRVAHIIADAIYERIT
GEKGYFDTRIVYVAESGPKNARQRQLAIMDQDGANSRALTNSNDIVLTPRFSPNRQEITYMSFENQQPRVYLLQLETGQR
EVVGNFPGMTFAPRFSPDGQRVIMSLQQEGNANIYTMDLRSRTTTRLTNTAAIDTSPSYSPDGSRVVFESDRGGRQQLYV
MGADGSGQTRISFGDGSYSTPVWSPRGDLIAFTKQSGGKFSIGVMKPDGSGERILTTGFHNEGPTWAPNGRVLMFFRQNA
GAGGPQLYSIDLTGYNEQLVPTQGFASDPAWSPLME

Sequences:

>Translated_436_residues
MEMLRRNFFRLLMVLVAGCGLIASPAKALVEIDINKGNVEPLPIAITDFVQGELAQKISDVIAADLKRSGLFAPINKGAF
IEKVSNPDATPRFEDWKVINAQALVIGRVTKEGDGRLKAEFRLWDTFAGTQMLGQQFYTQPENWRRVAHIIADAIYERIT
GEKGYFDTRIVYVAESGPKNARQRQLAIMDQDGANSRALTNSNDIVLTPRFSPNRQEITYMSFENQQPRVYLLQLETGQR
EVVGNFPGMTFAPRFSPDGQRVIMSLQQEGNANIYTMDLRSRTTTRLTNTAAIDTSPSYSPDGSRVVFESDRGGRQQLYV
MGADGSGQTRISFGDGSYSTPVWSPRGDLIAFTKQSGGKFSIGVMKPDGSGERILTTGFHNEGPTWAPNGRVLMFFRQNA
GAGGPQLYSIDLTGYNEQLVPTQGFASDPAWSPLME
>Mature_436_residues
MEMLRRNFFRLLMVLVAGCGLIASPAKALVEIDINKGNVEPLPIAITDFVQGELAQKISDVIAADLKRSGLFAPINKGAF
IEKVSNPDATPRFEDWKVINAQALVIGRVTKEGDGRLKAEFRLWDTFAGTQMLGQQFYTQPENWRRVAHIIADAIYERIT
GEKGYFDTRIVYVAESGPKNARQRQLAIMDQDGANSRALTNSNDIVLTPRFSPNRQEITYMSFENQQPRVYLLQLETGQR
EVVGNFPGMTFAPRFSPDGQRVIMSLQQEGNANIYTMDLRSRTTTRLTNTAAIDTSPSYSPDGSRVVFESDRGGRQQLYV
MGADGSGQTRISFGDGSYSTPVWSPRGDLIAFTKQSGGKFSIGVMKPDGSGERILTTGFHNEGPTWAPNGRVLMFFRQNA
GAGGPQLYSIDLTGYNEQLVPTQGFASDPAWSPLME

Specific function: Involved in the tonB-independent uptake of proteins [H]

COG id: COG0823

COG function: function code U; Periplasmic component of the Tol biopolymer transport system

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tolB family [H]

Homologues:

Organism=Escherichia coli, GI1786961, Length=423, Percent_Identity=30.2600472813239, Blast_Score=184, Evalue=8e-48,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011659
- InterPro:   IPR014167
- InterPro:   IPR007195
- InterPro:   IPR015943 [H]

Pfam domain/function: PF07676 PD40; PF04052 TolB_N [H]

EC number: NA

Molecular weight: Translated: 48236; Mature: 48236

Theoretical pI: Translated: 7.50; Mature: 7.50

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEMLRRNFFRLLMVLVAGCGLIASPAKALVEIDINKGNVEPLPIAITDFVQGELAQKISD
CCHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCCCCEEEEEEHHHHHHHHHHHHHH
VIAADLKRSGLFAPINKGAFIEKVSNPDATPRFEDWKVINAQALVIGRVTKEGDGRLKAE
HHHHHHHHCCCEEECCCCCEEEECCCCCCCCCCCCEEEEEEEEEEEEEEEECCCCEEEEE
FRLWDTFAGTQMLGQQFYTQPENWRRVAHIIADAIYERITGEKGYFDTRIVYVAESGPKN
EEEEEHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEECCCCCC
ARQRQLAIMDQDGANSRALTNSNDIVLTPRFSPNRQEITYMSFENQQPRVYLLQLETGQR
CCCEEEEEEECCCCCCCEEECCCCEEEECCCCCCCCEEEEEEECCCCCEEEEEEECCCCE
EVVGNFPGMTFAPRFSPDGQRVIMSLQQEGNANIYTMDLRSRTTTRLTNTAAIDTSPSYS
EEEECCCCCEECCCCCCCHHHHHHHHHHCCCCCEEEEECCCCCEEEEECEEEECCCCCCC
PDGSRVVFESDRGGRQQLYVMGADGSGQTRISFGDGSYSTPVWSPRGDLIAFTKQSGGKF
CCCCEEEEECCCCCCEEEEEEECCCCCCEEEEECCCCCCCCCCCCCCCEEEEEECCCCEE
SIGVMKPDGSGERILTTGFHNEGPTWAPNGRVLMFFRQNAGAGGPQLYSIDLTGYNEQLV
EEEEECCCCCCCEEEEECCCCCCCCCCCCCCEEEEEECCCCCCCCEEEEEEECCCCCCCC
PTQGFASDPAWSPLME
CCCCCCCCCCCCCCCC
>Mature Secondary Structure
MEMLRRNFFRLLMVLVAGCGLIASPAKALVEIDINKGNVEPLPIAITDFVQGELAQKISD
CCHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCCCCEEEEEEHHHHHHHHHHHHHH
VIAADLKRSGLFAPINKGAFIEKVSNPDATPRFEDWKVINAQALVIGRVTKEGDGRLKAE
HHHHHHHHCCCEEECCCCCEEEECCCCCCCCCCCCEEEEEEEEEEEEEEEECCCCEEEEE
FRLWDTFAGTQMLGQQFYTQPENWRRVAHIIADAIYERITGEKGYFDTRIVYVAESGPKN
EEEEEHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEECCCCCC
ARQRQLAIMDQDGANSRALTNSNDIVLTPRFSPNRQEITYMSFENQQPRVYLLQLETGQR
CCCEEEEEEECCCCCCCEEECCCCEEEECCCCCCCCEEEEEEECCCCCEEEEEEECCCCE
EVVGNFPGMTFAPRFSPDGQRVIMSLQQEGNANIYTMDLRSRTTTRLTNTAAIDTSPSYS
EEEECCCCCEECCCCCCCHHHHHHHHHHCCCCCEEEEECCCCCEEEEECEEEECCCCCCC
PDGSRVVFESDRGGRQQLYVMGADGSGQTRISFGDGSYSTPVWSPRGDLIAFTKQSGGKF
CCCCEEEEECCCCCCEEEEEEECCCCCCEEEEECCCCCCCCCCCCCCCEEEEEECCCCEE
SIGVMKPDGSGERILTTGFHNEGPTWAPNGRVLMFFRQNAGAGGPQLYSIDLTGYNEQLV
EEEEECCCCCCCEEEEECCCCCCCCCCCCCCEEEEEECCCCCCCCEEEEEEECCCCCCCC
PTQGFASDPAWSPLME
CCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11481430 [H]