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The map label for this gene is yfiY [H]

Identifier: 150397690

GI number: 150397690

Start: 2575326

End: 2576276

Strand: Direct

Name: yfiY [H]

Synonym: Smed_2491

Alternate gene names: 150397690

Gene position: 2575326-2576276 (Clockwise)

Preceding gene: 150397689

Following gene: 150397691

Centisome position: 68.1

GC content: 56.47

Gene sequence:

>951_bases
ATGAACGAAAATCTCATATTTGGATTCTTCATGCGCTTTGTTGTGTTGGCTTTACTAGGCATCATGCTGTCTTCTCCTGT
TTTCGCTCAATGTGACGGTAGGCTTATCGCCGAAGGGGCGTTCCTCCACCCACCGCTGTGCGCTCCCAAGGAGCCGAAAC
GGATCATATCTCTAGTCCCGACGTTCAGTCTTGGCATAGCGTTGGAGCTTGGCCTACCTGTCGTTGGCGCTCCGCTTTTC
GGGATGAGCGATGAGGATCTGAAGTCGAAAGCTGAGGCGGCGGGTGTGGTGGATCTCGGTTCTTTCTTGGAGCCCAGCAT
CGAGAAGATCGTCGCGCTTCAGCCCGATCTCATCATCGGCAGCAGCTTCCTGGGCGAGGAAGCATACCAGATGGCATCGC
GATTGGCTCCCACCGCGCTGCTCACCGGAGCGAACTGGAAAGAATTCTATCGGACGCTGGCAAATATCGCTGGCGTCGAC
GATGGAATTGAAGGTCGCTTCGCTGAATACGAGAAGCGGGTTGCGGCAGTTCGCGAGCGGGTTCCCAAGGATTTGCGAGT
ATCGGTGGTCCGCATCACGCCCTGGGAATTCCAGGTCTTTCTCGATGAACCCAATGCATGGGCGCCGTTCGACGTGCTGC
GCAAGGCGGGTGTCAGGCGTACAGATTTCGAGACATCCGAAACGAATGTCGGAACGAAACGCATCGATTTCGAGGGACTG
GCCGGCCTGGATGGGGATATCCTGCTCTACATCGTCGGCGGTTCCAACGATTCAGCCACCAGCGGCAGGCATGAGGCCGT
GCTGACGAACCCATTGTGGAAAATGCTGCCGGCCGTCAAAGCGGGCCGCGTTTATCGAGTAGACGCAGGCACCTGGATAG
AGTTCAACGGTCTGGCGTCGGCCAATAAGATTCTCGATGACATCGAGACTTACATCATCCCGAGGCCGTGA

Upstream 100 bases:

>100_bases
ACATAAGCGGAGCCGGCGTCGCGCGTGGCTATCTGAACCGCCCGAAAGGCAGCAAGCCTTTCTCGGCATTGCGCAGATCG
GCCCGCCAAGCTATTTTAAC

Downstream 100 bases:

>100_bases
AATGGTGGTCATAGAGTCGGCGCGGCGCAACACCCCCGCGACGCGGATCTTTGCTTTTGGCCTGTTTGCGCTGCTGCTCG
CTCTGCTGAGCCTGTGGTCA

Product: periplasmic binding protein

Products: ADP; phosphate; iron dicitrate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 316; Mature: 316

Protein sequence:

>316_residues
MNENLIFGFFMRFVVLALLGIMLSSPVFAQCDGRLIAEGAFLHPPLCAPKEPKRIISLVPTFSLGIALELGLPVVGAPLF
GMSDEDLKSKAEAAGVVDLGSFLEPSIEKIVALQPDLIIGSSFLGEEAYQMASRLAPTALLTGANWKEFYRTLANIAGVD
DGIEGRFAEYEKRVAAVRERVPKDLRVSVVRITPWEFQVFLDEPNAWAPFDVLRKAGVRRTDFETSETNVGTKRIDFEGL
AGLDGDILLYIVGGSNDSATSGRHEAVLTNPLWKMLPAVKAGRVYRVDAGTWIEFNGLASANKILDDIETYIIPRP

Sequences:

>Translated_316_residues
MNENLIFGFFMRFVVLALLGIMLSSPVFAQCDGRLIAEGAFLHPPLCAPKEPKRIISLVPTFSLGIALELGLPVVGAPLF
GMSDEDLKSKAEAAGVVDLGSFLEPSIEKIVALQPDLIIGSSFLGEEAYQMASRLAPTALLTGANWKEFYRTLANIAGVD
DGIEGRFAEYEKRVAAVRERVPKDLRVSVVRITPWEFQVFLDEPNAWAPFDVLRKAGVRRTDFETSETNVGTKRIDFEGL
AGLDGDILLYIVGGSNDSATSGRHEAVLTNPLWKMLPAVKAGRVYRVDAGTWIEFNGLASANKILDDIETYIIPRP
>Mature_316_residues
MNENLIFGFFMRFVVLALLGIMLSSPVFAQCDGRLIAEGAFLHPPLCAPKEPKRIISLVPTFSLGIALELGLPVVGAPLF
GMSDEDLKSKAEAAGVVDLGSFLEPSIEKIVALQPDLIIGSSFLGEEAYQMASRLAPTALLTGANWKEFYRTLANIAGVD
DGIEGRFAEYEKRVAAVRERVPKDLRVSVVRITPWEFQVFLDEPNAWAPFDVLRKAGVRRTDFETSETNVGTKRIDFEGL
AGLDGDILLYIVGGSNDSATSGRHEAVLTNPLWKMLPAVKAGRVYRVDAGTWIEFNGLASANKILDDIETYIIPRP

Specific function: Part of the ABC transporter complex yfiYZ/yfhA/yusV involved in import of the iron-hydroxamate siderophores schizokinen, arthrobactin and corprogen. Binds the siderophores and delivers them to the surface of yfiZ/yfhA (Probable) [H]

COG id: COG0614

COG function: function code P; ABC-type Fe3+-hydroxamate transport system, periplasmic component

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable). Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Fe/B12 periplasmic-binding domain [H]

Homologues:

Organism=Escherichia coli, GI87082411, Length=276, Percent_Identity=26.4492753623188, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1786807, Length=285, Percent_Identity=25.6140350877193, Blast_Score=65, Evalue=6e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002491 [H]

Pfam domain/function: PF01497 Peripla_BP_2 [H]

EC number: NA

Molecular weight: Translated: 34557; Mature: 34557

Theoretical pI: Translated: 4.71; Mature: 4.71

Prosite motif: PS50983 FE_B12_PBP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNENLIFGFFMRFVVLALLGIMLSSPVFAQCDGRLIAEGAFLHPPLCAPKEPKRIISLVP
CCCCEEHHHHHHHHHHHHHHHHHCCCCEECCCCEEEECCCCCCCCCCCCCCHHHHHHHCC
TFSLGIALELGLPVVGAPLFGMSDEDLKSKAEAAGVVDLGSFLEPSIEKIVALQPDLIIG
HHHHCEEEEECCCEECCCCCCCCCHHHHHHHHHCCHHHHHHHHCHHHHHHHHCCCCEEEC
SSFLGEEAYQMASRLAPTALLTGANWKEFYRTLANIAGVDDGIEGRFAEYEKRVAAVRER
CHHHHHHHHHHHHHCCCHHEEECCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHH
VPKDLRVSVVRITPWEFQVFLDEPNAWAPFDVLRKAGVRRTDFETSETNVGTKRIDFEGL
CCCCCEEEEEEECCEEEEEEEECCCCCCHHHHHHHCCCCCCCCCCCCCCCCCEEECCCCC
AGLDGDILLYIVGGSNDSATSGRHEAVLTNPLWKMLPAVKAGRVYRVDAGTWIEFNGLAS
CCCCCCEEEEEEECCCCCCCCCCCCEEEECHHHHHHHHHHCCCEEEECCCCEEEECCCCH
ANKILDDIETYIIPRP
HHHHHHHHHHHCCCCC
>Mature Secondary Structure
MNENLIFGFFMRFVVLALLGIMLSSPVFAQCDGRLIAEGAFLHPPLCAPKEPKRIISLVP
CCCCEEHHHHHHHHHHHHHHHHHCCCCEECCCCEEEECCCCCCCCCCCCCCHHHHHHHCC
TFSLGIALELGLPVVGAPLFGMSDEDLKSKAEAAGVVDLGSFLEPSIEKIVALQPDLIIG
HHHHCEEEEECCCEECCCCCCCCCHHHHHHHHHCCHHHHHHHHCHHHHHHHHCCCCEEEC
SSFLGEEAYQMASRLAPTALLTGANWKEFYRTLANIAGVDDGIEGRFAEYEKRVAAVRER
CHHHHHHHHHHHHHCCCHHEEECCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHH
VPKDLRVSVVRITPWEFQVFLDEPNAWAPFDVLRKAGVRRTDFETSETNVGTKRIDFEGL
CCCCCEEEEEEECCEEEEEEEECCCCCCHHHHHHHCCCCCCCCCCCCCCCCCEEECCCCC
AGLDGDILLYIVGGSNDSATSGRHEAVLTNPLWKMLPAVKAGRVYRVDAGTWIEFNGLAS
CCCCCCEEEEEEECCCCCCCCCCCCEEEECHHHHHHHHHHCCCEEEECCCCEEEECCCCH
ANKILDDIETYIIPRP
HHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; iron dicitrate [Periplasm]; H2O [C]

Specific reaction: ATP + iron dicitrate [Periplasm] + H2O = ADP + phosphate + iron dicitrate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8946165; 9384377 [H]