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The map label for this gene is puuA [H]

Identifier: 150397616

GI number: 150397616

Start: 2493827

End: 2495194

Strand: Reverse

Name: puuA [H]

Synonym: Smed_2416

Alternate gene names: 150397616

Gene position: 2495194-2493827 (Counterclockwise)

Preceding gene: 150397631

Following gene: 150397615

Centisome position: 65.98

GC content: 64.84

Gene sequence:

>1368_bases
ATGTCATTTGATGTGGACGACACGGAAGCCTTTCAGGCCTGGATCGATCAGAACGGCCCGATAGAGAGTATTCAGGCGGT
CATTTGCGACCTGAACGGGATCATGCGCGGCAAGCGCGTGCCGGTGGAGCAGGCCGCGAAGGTGCTCGGCGGCGGTATCC
GCATGCCGCTTTCGATCGTCGGCGTCGACGTTTGGGGCGAGGACATCATCGGCAGCGAACAGGTCTTTGCGACCGGCGAT
CGCGACGGCATCTGCGGTGTGACCGGGCGAGGGGCCCTGCCGGTCAACTGGACGTCGCGGCCGAGCGCGCTGGTTCCGCT
ATGGCTCTTCGTAGAGGACGGCAGACCCTTTCTCGCCGACCCGCGGCAGGCCCTCGCGGCAATCGTGCGCGAATATCGCG
AACTGGGCCTTCGCCCGGTGGTGGCGACGGAGCTTGAATTTTACCTCATAGATCCGGAGCCGGACAGCGCCGTCCCGCCG
ATCTCGCCTTATACCGGCAAGCGGCTCGATTCCGACGCTATCCTTTCGATCGACGAGCTCGACGACTTCGGCGAGTTCTT
CTCCGACGTCTACAGGGAGTGCGCGCGGCAGAACGTTCCGGCGGATGCGGCGATCGCGGAGAACGGCATCGGCCAGTTCG
AGATCAATCTGCTGCACAGCGACGATCCCCTGAAGGCGGCGGACGATGCGATCTTCTTCAAGCGTATCGTCAAGGGCGTC
GCCCGCAAGCACGGTCTCGCCGCCACCTTCATGGCGAAGCCCTACGGCACCCGCTCCGGCAACGGCATGCACATTCATTT
CAGCCTGCTCGACGAGGAGGGCAACAACGTCTTCGATGATGGCAGCGACGAGGGGGCGGCCGTGCTCAAACATGCGGTGG
CCGGCCTCCTGCGCGGAATGGCCGAGACGACGCTGATGTTCGCCCCGCATTTCAATTCCTACCGGCGGCTGCGGCCCGAC
ACGCATGCGCCGACCTCGATTTCCTGGGGCTACGAGAACCGCACCTCGGCAATCCGCATCCCGGGGGGCAATCCGGCGGC
GCGGCGGATCGAACACCGCGTCGCCGGTGCGGACGCCAACCCCTATCTCGTCATTGCCGCTATCCTTGGAGCGGCGCTAG
TCGGCATCCGCAACAAGTGGAAACCGCCGGTGCCGGTCGAGGGGCGTGCCTATGCGACCGAGCGGCTGCCCAAGATACCT
GCCGACTGGGGGCAGGCGGTCGACGCCTTCGAGGCCGGCCCGATCGCCGCCGAGATATTCGATCCCGTGCTTCGCTCCAT
GCTGATCGCCTGCAAGCGCCAGGAGATCGCCGGCTTCGCCGAGCAGGTCACGGACTACGAGTTTAGCGCCTATCTGGAAA
TCGTCTAA

Upstream 100 bases:

>100_bases
GTCGTTGGATCCAGCCGATCCTGTCGGCTCGCTTCAGCTTGACTCTAAATCATCAAAGATGTCAATTTCTGATAAATGTG
ATCACAAAAAGAAATTCGAT

Downstream 100 bases:

>100_bases
TGGCAGAGAAGCAGACATCCTATGCAGGGAACGGCAGCTATCCGACGAGCTATTACGCCGCCTCGCGCAATATCATCCGC
AACCCGGTGCGTCTGGAAGG

Product: glutamine synthetase catalytic subunit

Products: NA

Alternate protein names: Gamma-Glu-Put synthetase; Glutamate--putrescine ligase [H]

Number of amino acids: Translated: 455; Mature: 454

Protein sequence:

>455_residues
MSFDVDDTEAFQAWIDQNGPIESIQAVICDLNGIMRGKRVPVEQAAKVLGGGIRMPLSIVGVDVWGEDIIGSEQVFATGD
RDGICGVTGRGALPVNWTSRPSALVPLWLFVEDGRPFLADPRQALAAIVREYRELGLRPVVATELEFYLIDPEPDSAVPP
ISPYTGKRLDSDAILSIDELDDFGEFFSDVYRECARQNVPADAAIAENGIGQFEINLLHSDDPLKAADDAIFFKRIVKGV
ARKHGLAATFMAKPYGTRSGNGMHIHFSLLDEEGNNVFDDGSDEGAAVLKHAVAGLLRGMAETTLMFAPHFNSYRRLRPD
THAPTSISWGYENRTSAIRIPGGNPAARRIEHRVAGADANPYLVIAAILGAALVGIRNKWKPPVPVEGRAYATERLPKIP
ADWGQAVDAFEAGPIAAEIFDPVLRSMLIACKRQEIAGFAEQVTDYEFSAYLEIV

Sequences:

>Translated_455_residues
MSFDVDDTEAFQAWIDQNGPIESIQAVICDLNGIMRGKRVPVEQAAKVLGGGIRMPLSIVGVDVWGEDIIGSEQVFATGD
RDGICGVTGRGALPVNWTSRPSALVPLWLFVEDGRPFLADPRQALAAIVREYRELGLRPVVATELEFYLIDPEPDSAVPP
ISPYTGKRLDSDAILSIDELDDFGEFFSDVYRECARQNVPADAAIAENGIGQFEINLLHSDDPLKAADDAIFFKRIVKGV
ARKHGLAATFMAKPYGTRSGNGMHIHFSLLDEEGNNVFDDGSDEGAAVLKHAVAGLLRGMAETTLMFAPHFNSYRRLRPD
THAPTSISWGYENRTSAIRIPGGNPAARRIEHRVAGADANPYLVIAAILGAALVGIRNKWKPPVPVEGRAYATERLPKIP
ADWGQAVDAFEAGPIAAEIFDPVLRSMLIACKRQEIAGFAEQVTDYEFSAYLEIV
>Mature_454_residues
SFDVDDTEAFQAWIDQNGPIESIQAVICDLNGIMRGKRVPVEQAAKVLGGGIRMPLSIVGVDVWGEDIIGSEQVFATGDR
DGICGVTGRGALPVNWTSRPSALVPLWLFVEDGRPFLADPRQALAAIVREYRELGLRPVVATELEFYLIDPEPDSAVPPI
SPYTGKRLDSDAILSIDELDDFGEFFSDVYRECARQNVPADAAIAENGIGQFEINLLHSDDPLKAADDAIFFKRIVKGVA
RKHGLAATFMAKPYGTRSGNGMHIHFSLLDEEGNNVFDDGSDEGAAVLKHAVAGLLRGMAETTLMFAPHFNSYRRLRPDT
HAPTSISWGYENRTSAIRIPGGNPAARRIEHRVAGADANPYLVIAAILGAALVGIRNKWKPPVPVEGRAYATERLPKIPA
DWGQAVDAFEAGPIAAEIFDPVLRSMLIACKRQEIAGFAEQVTDYEFSAYLEIV

Specific function: Involved in the breakdown of putrescine via the biosynthesis of gamma-L-glutamylputrescine [H]

COG id: COG0174

COG function: function code E; Glutamine synthetase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamine synthetase family [H]

Homologues:

Organism=Homo sapiens, GI221307485, Length=418, Percent_Identity=23.9234449760766, Blast_Score=79, Evalue=7e-15,
Organism=Escherichia coli, GI87081870, Length=456, Percent_Identity=37.5, Blast_Score=296, Evalue=2e-81,
Organism=Escherichia coli, GI1790301, Length=196, Percent_Identity=34.6938775510204, Blast_Score=107, Evalue=1e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008147
- InterPro:   IPR014746
- InterPro:   IPR008146 [H]

Pfam domain/function: PF00120 Gln-synt_C [H]

EC number: =6.3.1.11 [H]

Molecular weight: Translated: 49556; Mature: 49424

Theoretical pI: Translated: 4.64; Mature: 4.64

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSFDVDDTEAFQAWIDQNGPIESIQAVICDLNGIMRGKRVPVEQAAKVLGGGIRMPLSIV
CCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCEEEE
GVDVWGEDIIGSEQVFATGDRDGICGVTGRGALPVNWTSRPSALVPLWLFVEDGRPFLAD
EEEECCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEEECCCCCCCC
PRQALAAIVREYRELGLRPVVATELEFYLIDPEPDSAVPPISPYTGKRLDSDAILSIDEL
HHHHHHHHHHHHHHCCCCCEEEECEEEEEECCCCCCCCCCCCCCCCCCCCCCCEEEHHHH
DDFGEFFSDVYRECARQNVPADAAIAENGIGQFEINLLHSDDPLKAADDAIFFKRIVKGV
HHHHHHHHHHHHHHHHCCCCCCHHHHHCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHH
ARKHGLAATFMAKPYGTRSGNGMHIHFSLLDEEGNNVFDDGSDEGAAVLKHAVAGLLRGM
HHHCCCEEEEEECCCCCCCCCEEEEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHH
AETTLMFAPHFNSYRRLRPDTHAPTSISWGYENRTSAIRIPGGNPAARRIEHRVAGADAN
HHHHEEECCCCCCHHHCCCCCCCCCEEECCCCCCCCEEECCCCCHHHHHHHHHHCCCCCC
PYLVIAAILGAALVGIRNKWKPPVPVEGRAYATERLPKIPADWGQAVDAFEAGPIAAEIF
CHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHCCCCCCHHHHHHHHHCCCCHHHHHH
DPVLRSMLIACKRQEIAGFAEQVTDYEFSAYLEIV
HHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEC
>Mature Secondary Structure 
SFDVDDTEAFQAWIDQNGPIESIQAVICDLNGIMRGKRVPVEQAAKVLGGGIRMPLSIV
CCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCEEEE
GVDVWGEDIIGSEQVFATGDRDGICGVTGRGALPVNWTSRPSALVPLWLFVEDGRPFLAD
EEEECCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEEECCCCCCCC
PRQALAAIVREYRELGLRPVVATELEFYLIDPEPDSAVPPISPYTGKRLDSDAILSIDEL
HHHHHHHHHHHHHHCCCCCEEEECEEEEEECCCCCCCCCCCCCCCCCCCCCCCEEEHHHH
DDFGEFFSDVYRECARQNVPADAAIAENGIGQFEINLLHSDDPLKAADDAIFFKRIVKGV
HHHHHHHHHHHHHHHHCCCCCCHHHHHCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHH
ARKHGLAATFMAKPYGTRSGNGMHIHFSLLDEEGNNVFDDGSDEGAAVLKHAVAGLLRGM
HHHCCCEEEEEECCCCCCCCCEEEEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHH
AETTLMFAPHFNSYRRLRPDTHAPTSISWGYENRTSAIRIPGGNPAARRIEHRVAGADAN
HHHHEEECCCCCCHHHCCCCCCCCCEEECCCCCCCCEEECCCCCHHHHHHHHHHCCCCCC
PYLVIAAILGAALVGIRNKWKPPVPVEGRAYATERLPKIPADWGQAVDAFEAGPIAAEIF
CHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHCCCCCCHHHHHHHHHCCCCHHHHHH
DPVLRSMLIACKRQEIAGFAEQVTDYEFSAYLEIV
HHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]