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The map label for this gene is puuB [H]

Identifier: 150397615

GI number: 150397615

Start: 2492517

End: 2493827

Strand: Reverse

Name: puuB [H]

Synonym: Smed_2415

Alternate gene names: 150397615

Gene position: 2493827-2492517 (Counterclockwise)

Preceding gene: 150397616

Following gene: 150397614

Centisome position: 65.94

GC content: 62.32

Gene sequence:

>1311_bases
ATGGCAGAGAAGCAGACATCCTATGCAGGGAACGGCAGCTATCCGACGAGCTATTACGCCGCCTCGCGCAATATCATCCG
CAACCCGGTGCGTCTGGAAGGCCGGATCGAGACCGACATCTGCATCGTCGGAGCGGGCTATTCGGGCCTGTCCACCGCCA
TCCATCTCGCCGAAAAGGGCTACAAGGTCACCGTGGTGGAGGGGGCGCAGGTGGGCTGGGGCGCGTCCGGACGAAATGGC
GGTCAAATCGTCAACGGCCTCAATGCGGGTTTGTCGACGATCGAGCGCCGCTACGGCGAGGATGCCGCCCGCTTCATCGG
CGGTCTGGTGCAGGAGGGCGGGCGGATCATCCGCCGGCTTGTCGAGCGGTATCAGATCGATTGCGATCTGAAGCCCGGCA
ACATCTATGCCGCCTATACCGGAGCGCATATGAAGGAGCTGGAGGCCAAACAGGCGCTCTGGCGCAAATATGGGATGGAC
GATCATCAGCTTCTCGACCGTGAAGCGCTGCGCAGGTTCGTGGTTTCGGATGCCTATTGCGGCGGCATGCTCGACATGAC
CGGCGGCCACATGCATCCGCTCAATCTAGTGCTCGGAGAGGCACGCGCTTTCGAGAGCCTCGGCGGTGTGATCTACGAAA
TGTCGCCGGTGACCCGCGTCGAACACGAGGCGGCGCGGCCGGTCGTGCACACGAAAGGCGGTGAGGTTTCCGCCCGCATC
GTCGTGCTTTGCGGCAACGCTTATCTCGGCGATGCAGTGCCGAAGCTCGCCACCCGCGTCATGCCCGTTTCGACCCAGAT
GATCACCACCGAGCAGCTCGGTGAGGAACGCGCCCATTCGCTGATTCCGAGCGACATGTGCGTGGAGGATGTCCGCTACA
TCCTCGACTATTTCCGGCTGTCTGCCGACAAGCGGATGATTTTCGGCGGCGGCACCGTCTATGGCGGCACAGACCCGGCG
GACGTCGTCGCAAAACTCAGGCCCAACCTCGAAAAGGTCTTTCCGCGCCTGAAGGGCGTGAAGATCGACTATGCCTGGAG
CGGCAATTTCGCGCTCTCCTTCACGCGCGTGCCGCAAATGGGCCGGATCGGCAGCAACACCTATTTCGCCCATGGCTATA
GCGGTCACGGCGTGACAGGCTCCCACCTGTTCGGCCGCATCCTTGCCGAAGCGATAAACGGCGACGCCTCACGCTTCGAT
GCCTTCGAGAAAATGCCGTGGTACCCGTTCCCCGGCGGGCGCATGTTACGGGCGCAATATTCGACGCTCGGGTCGTGGTG
GTACATGTTCAAGGACGCAGTCGGCTGGTAG

Upstream 100 bases:

>100_bases
TGCTTCGCTCCATGCTGATCGCCTGCAAGCGCCAGGAGATCGCCGGCTTCGCCGAGCAGGTCACGGACTACGAGTTTAGC
GCCTATCTGGAAATCGTCTA

Downstream 100 bases:

>100_bases
CTGGTTCGCTAAGCGCGAGCCGCCGGTCTTTTCACGGCAAGTTTTCAGTGCGAAACCGCACTATTCCGCACTTCATGGCG
CCTGGTTAAACGAATTTTTC

Product: FAD dependent oxidoreductase

Products: NA

Alternate protein names: Gamma-Glu-Put oxidase; Gamma-glutamylputrescine oxidase [H]

Number of amino acids: Translated: 436; Mature: 435

Protein sequence:

>436_residues
MAEKQTSYAGNGSYPTSYYAASRNIIRNPVRLEGRIETDICIVGAGYSGLSTAIHLAEKGYKVTVVEGAQVGWGASGRNG
GQIVNGLNAGLSTIERRYGEDAARFIGGLVQEGGRIIRRLVERYQIDCDLKPGNIYAAYTGAHMKELEAKQALWRKYGMD
DHQLLDREALRRFVVSDAYCGGMLDMTGGHMHPLNLVLGEARAFESLGGVIYEMSPVTRVEHEAARPVVHTKGGEVSARI
VVLCGNAYLGDAVPKLATRVMPVSTQMITTEQLGEERAHSLIPSDMCVEDVRYILDYFRLSADKRMIFGGGTVYGGTDPA
DVVAKLRPNLEKVFPRLKGVKIDYAWSGNFALSFTRVPQMGRIGSNTYFAHGYSGHGVTGSHLFGRILAEAINGDASRFD
AFEKMPWYPFPGGRMLRAQYSTLGSWWYMFKDAVGW

Sequences:

>Translated_436_residues
MAEKQTSYAGNGSYPTSYYAASRNIIRNPVRLEGRIETDICIVGAGYSGLSTAIHLAEKGYKVTVVEGAQVGWGASGRNG
GQIVNGLNAGLSTIERRYGEDAARFIGGLVQEGGRIIRRLVERYQIDCDLKPGNIYAAYTGAHMKELEAKQALWRKYGMD
DHQLLDREALRRFVVSDAYCGGMLDMTGGHMHPLNLVLGEARAFESLGGVIYEMSPVTRVEHEAARPVVHTKGGEVSARI
VVLCGNAYLGDAVPKLATRVMPVSTQMITTEQLGEERAHSLIPSDMCVEDVRYILDYFRLSADKRMIFGGGTVYGGTDPA
DVVAKLRPNLEKVFPRLKGVKIDYAWSGNFALSFTRVPQMGRIGSNTYFAHGYSGHGVTGSHLFGRILAEAINGDASRFD
AFEKMPWYPFPGGRMLRAQYSTLGSWWYMFKDAVGW
>Mature_435_residues
AEKQTSYAGNGSYPTSYYAASRNIIRNPVRLEGRIETDICIVGAGYSGLSTAIHLAEKGYKVTVVEGAQVGWGASGRNGG
QIVNGLNAGLSTIERRYGEDAARFIGGLVQEGGRIIRRLVERYQIDCDLKPGNIYAAYTGAHMKELEAKQALWRKYGMDD
HQLLDREALRRFVVSDAYCGGMLDMTGGHMHPLNLVLGEARAFESLGGVIYEMSPVTRVEHEAARPVVHTKGGEVSARIV
VLCGNAYLGDAVPKLATRVMPVSTQMITTEQLGEERAHSLIPSDMCVEDVRYILDYFRLSADKRMIFGGGTVYGGTDPAD
VVAKLRPNLEKVFPRLKGVKIDYAWSGNFALSFTRVPQMGRIGSNTYFAHGYSGHGVTGSHLFGRILAEAINGDASRFDA
FEKMPWYPFPGGRMLRAQYSTLGSWWYMFKDAVGW

Specific function: Involved in the breakdown of putrescine via the oxidation of L-glutamylputrescine [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1787559, Length=423, Percent_Identity=51.7730496453901, Blast_Score=466, Evalue=1e-132,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006076 [H]

Pfam domain/function: PF01266 DAO [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 47953; Mature: 47822

Theoretical pI: Translated: 8.60; Mature: 8.60

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEKQTSYAGNGSYPTSYYAASRNIIRNPVRLEGRIETDICIVGAGYSGLSTAIHLAEKG
CCCCCCCCCCCCCCCCHHHHHHHHHHCCCEEECCEECCCEEEEECCCHHHHHHHHHHHCC
YKVTVVEGAQVGWGASGRNGGQIVNGLNAGLSTIERRYGEDAARFIGGLVQEGGRIIRRL
EEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHH
VERYQIDCDLKPGNIYAAYTGAHMKELEAKQALWRKYGMDDHQLLDREALRRFVVSDAYC
HHHHCCCEECCCCCEEEEECCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCC
GGMLDMTGGHMHPLNLVLGEARAFESLGGVIYEMSPVTRVEHEAARPVVHTKGGEVSARI
CCCEECCCCCCCHHHHHHHHHHHHHHHCCEEEECCCCHHHHHHHCCCEEECCCCCEEEEE
VVLCGNAYLGDAVPKLATRVMPVSTQMITTEQLGEERAHSLIPSDMCVEDVRYILDYFRL
EEEECCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
SADKRMIFGGGTVYGGTDPADVVAKLRPNLEKVFPRLKGVKIDYAWSGNFALSFTRVPQM
CCCCEEEEECCEECCCCCHHHHHHHHCCCHHHHHHHHCCCEEEEEECCCEEEEEECCCCC
GRIGSNTYFAHGYSGHGVTGSHLFGRILAEAINGDASRFDAFEKMPWYPFPGGRMLRAQY
CCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCCEEHHHH
STLGSWWYMFKDAVGW
HHHHHHHHHHHHHCCC
>Mature Secondary Structure 
AEKQTSYAGNGSYPTSYYAASRNIIRNPVRLEGRIETDICIVGAGYSGLSTAIHLAEKG
CCCCCCCCCCCCCCCHHHHHHHHHHCCCEEECCEECCCEEEEECCCHHHHHHHHHHHCC
YKVTVVEGAQVGWGASGRNGGQIVNGLNAGLSTIERRYGEDAARFIGGLVQEGGRIIRRL
EEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHH
VERYQIDCDLKPGNIYAAYTGAHMKELEAKQALWRKYGMDDHQLLDREALRRFVVSDAYC
HHHHCCCEECCCCCEEEEECCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCC
GGMLDMTGGHMHPLNLVLGEARAFESLGGVIYEMSPVTRVEHEAARPVVHTKGGEVSARI
CCCEECCCCCCCHHHHHHHHHHHHHHHCCEEEECCCCHHHHHHHCCCEEECCCCCEEEEE
VVLCGNAYLGDAVPKLATRVMPVSTQMITTEQLGEERAHSLIPSDMCVEDVRYILDYFRL
EEEECCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
SADKRMIFGGGTVYGGTDPADVVAKLRPNLEKVFPRLKGVKIDYAWSGNFALSFTRVPQM
CCCCEEEEECCEECCCCCHHHHHHHHCCCHHHHHHHHCCCEEEEEECCCEEEEEECCCCC
GRIGSNTYFAHGYSGHGVTGSHLFGRILAEAINGDASRFDAFEKMPWYPFPGGRMLRAQY
CCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCCEEHHHH
STLGSWWYMFKDAVGW
HHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503; 1840553; 7984428 [H]