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The map label for this gene is purR [H]

Identifier: 150397516

GI number: 150397516

Start: 2395596

End: 2396600

Strand: Reverse

Name: purR [H]

Synonym: Smed_2316

Alternate gene names: 150397516

Gene position: 2396600-2395596 (Counterclockwise)

Preceding gene: 150397519

Following gene: 150397515

Centisome position: 63.37

GC content: 62.39

Gene sequence:

>1005_bases
ATGAGCACAATCGCAAAAGTGGCAGCAAGGGCTGGCGTGTCGCCGACGACGGTTTCTCACGTGATCAATCATAGCGATCG
CGTGTCGAAACCCCTGCGCGAGCGCGTCGAGGCGGCGATCAAGGATCTCGGTTACAAACCCAATCCGCAGGCGCAGAGCC
TGCGGACGGGGCGCACCAATATCGTCGCAATGCTGATCCCCGACATCCGCAACCCGTTTTATCCGGAGCTCGTCAAGGCA
GCGCAGTCGGAGCTTGAAGTTGCCGGTCTGGACATGCTGATCTTCAACACCGACGTTCCGGGCGGTCAGTCCCAGGATCA
TGGGCGCGAATACCTGCGCCAGATCGGCAACAAGCGCGTCGATGGTCTCATCGTCGGCGACTTTGCCCTGCACGGCATGC
ATGACGCGCTGATGCGCGTCGAGACGCCGACCGTCTTCATTGGCAACCTGCCGAATCACGCCGTGGATTGCGTCCGGATC
GACGATTTCGGCGGCGGCTACCAGATGGGTGCTTACCTGGCCGGGAAGGGCCATCGGCGCATTGCCCATGTAACCGGTCC
GTCATTCTTCGCCGAAGCGATGTCGCGGGCGGATGGCTTCGAACAGGGATTGGCCGACCATGGTGCAACGCCCGATCCCG
GTTTGCGCTTCGAGGGCAGCTATCTTGCGCCTTCCGGCAAGGCAGCCGCCGAATGGCTGCTTGCCGGACACGGCGGTGAT
CTGCCGTCGGCAGTCTTCTTCGGCAACTATCTGATGGCAGCTGGCGCTTTGGCCGAGTTCTACGACCGCGGCATTCGCAT
ACCCGACGATTTCAAGGTCGCAGTATTCGGCGATCAGCCGCAGCTCGAATATGTCCGGCCGCGGCTGACCCGCGTCGGCA
ACGCGCCGGCCGAACTGGCGCGCCGCGCAACTCGCATGCTGCTCGAACGGATCAACGGAACCTATGAGGGAGAGCCGAGA
AGCGAGGTCGTTTCATGCGTGCTGCGGGAGTTCGAGACAGCGTGA

Upstream 100 bases:

>100_bases
CGTCGCTCCCCTCATGCTTGAATGCCACGCCGGTTGAAAGAGTCACGACGGTAAAAAAAATCGATTTGCAAATCGATTTT
TTTGCGTAATGTTGGCGTCC

Downstream 100 bases:

>100_bases
CATCGCTCAAGTGAGGCGGCCCTGAGGAGAGGGCCTGAACAAGTGTGAGAGGGAGGTACCAATGAAGAATAGATCCAGCC
TTGTTGCGGCATCCGGCAAG

Product: alanine racemase

Products: NA

Alternate protein names: Pur regulon repressor; Purine nucleotide synthesis repressor [H]

Number of amino acids: Translated: 334; Mature: 333

Protein sequence:

>334_residues
MSTIAKVAARAGVSPTTVSHVINHSDRVSKPLRERVEAAIKDLGYKPNPQAQSLRTGRTNIVAMLIPDIRNPFYPELVKA
AQSELEVAGLDMLIFNTDVPGGQSQDHGREYLRQIGNKRVDGLIVGDFALHGMHDALMRVETPTVFIGNLPNHAVDCVRI
DDFGGGYQMGAYLAGKGHRRIAHVTGPSFFAEAMSRADGFEQGLADHGATPDPGLRFEGSYLAPSGKAAAEWLLAGHGGD
LPSAVFFGNYLMAAGALAEFYDRGIRIPDDFKVAVFGDQPQLEYVRPRLTRVGNAPAELARRATRMLLERINGTYEGEPR
SEVVSCVLREFETA

Sequences:

>Translated_334_residues
MSTIAKVAARAGVSPTTVSHVINHSDRVSKPLRERVEAAIKDLGYKPNPQAQSLRTGRTNIVAMLIPDIRNPFYPELVKA
AQSELEVAGLDMLIFNTDVPGGQSQDHGREYLRQIGNKRVDGLIVGDFALHGMHDALMRVETPTVFIGNLPNHAVDCVRI
DDFGGGYQMGAYLAGKGHRRIAHVTGPSFFAEAMSRADGFEQGLADHGATPDPGLRFEGSYLAPSGKAAAEWLLAGHGGD
LPSAVFFGNYLMAAGALAEFYDRGIRIPDDFKVAVFGDQPQLEYVRPRLTRVGNAPAELARRATRMLLERINGTYEGEPR
SEVVSCVLREFETA
>Mature_333_residues
STIAKVAARAGVSPTTVSHVINHSDRVSKPLRERVEAAIKDLGYKPNPQAQSLRTGRTNIVAMLIPDIRNPFYPELVKAA
QSELEVAGLDMLIFNTDVPGGQSQDHGREYLRQIGNKRVDGLIVGDFALHGMHDALMRVETPTVFIGNLPNHAVDCVRID
DFGGGYQMGAYLAGKGHRRIAHVTGPSFFAEAMSRADGFEQGLADHGATPDPGLRFEGSYLAPSGKAAAEWLLAGHGGDL
PSAVFFGNYLMAAGALAEFYDRGIRIPDDFKVAVFGDQPQLEYVRPRLTRVGNAPAELARRATRMLLERINGTYEGEPRS
EVVSCVLREFETA

Specific function: Is the main repressor of the genes involved in the de novo synthesis of purine nucleotides, regulating purB, purC, purEK, purF, purHD, purL, purMN and guaBA expression. PurR is allosterically activated to bind its cognate DNA by binding the purine corepre

COG id: COG1609

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790194, Length=315, Percent_Identity=33.6507936507937, Blast_Score=144, Evalue=9e-36,
Organism=Escherichia coli, GI1787948, Length=325, Percent_Identity=31.0769230769231, Blast_Score=136, Evalue=1e-33,
Organism=Escherichia coli, GI1790369, Length=332, Percent_Identity=27.4096385542169, Blast_Score=135, Evalue=3e-33,
Organism=Escherichia coli, GI1788474, Length=325, Percent_Identity=32.3076923076923, Blast_Score=127, Evalue=1e-30,
Organism=Escherichia coli, GI1789202, Length=294, Percent_Identity=31.9727891156463, Blast_Score=123, Evalue=2e-29,
Organism=Escherichia coli, GI1789068, Length=318, Percent_Identity=26.4150943396226, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1787580, Length=321, Percent_Identity=26.4797507788162, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI48994940, Length=317, Percent_Identity=24.9211356466877, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1787906, Length=264, Percent_Identity=31.4393939393939, Blast_Score=92, Evalue=6e-20,
Organism=Escherichia coli, GI1786540, Length=295, Percent_Identity=26.7796610169492, Blast_Score=82, Evalue=7e-17,
Organism=Escherichia coli, GI1786268, Length=328, Percent_Identity=24.390243902439, Blast_Score=78, Evalue=7e-16,
Organism=Escherichia coli, GI1790689, Length=330, Percent_Identity=22.1212121212121, Blast_Score=68, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 36300; Mature: 36168

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTIAKVAARAGVSPTTVSHVINHSDRVSKPLRERVEAAIKDLGYKPNPQAQSLRTGRTN
CCHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHCCCCC
IVAMLIPDIRNPFYPELVKAAQSELEVAGLDMLIFNTDVPGGQSQDHGREYLRQIGNKRV
EEEEEECCCCCCCCHHHHHHHHHHHHHHCCEEEEEECCCCCCCCHHHHHHHHHHHCCCCC
DGLIVGDFALHGMHDALMRVETPTVFIGNLPNHAVDCVRIDDFGGGYQMGAYLAGKGHRR
CEEEEEHHHHHHHHHHHHHCCCCEEEECCCCCCCEEEEEEECCCCCEEECEEEECCCCCE
IAHVTGPSFFAEAMSRADGFEQGLADHGATPDPGLRFEGSYLAPSGKAAAEWLLAGHGGD
EEEECCHHHHHHHHHHHCHHHHHHHHCCCCCCCCCEECCCEECCCCHHHHHHHHCCCCCC
LPSAVFFGNYLMAAGALAEFYDRGIRIPDDFKVAVFGDQPQLEYVRPRLTRVGNAPAELA
CCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCHHHHHHHHHHCCCCHHHHH
RRATRMLLERINGTYEGEPRSEVVSCVLREFETA
HHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure 
STIAKVAARAGVSPTTVSHVINHSDRVSKPLRERVEAAIKDLGYKPNPQAQSLRTGRTN
CHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHCCCCC
IVAMLIPDIRNPFYPELVKAAQSELEVAGLDMLIFNTDVPGGQSQDHGREYLRQIGNKRV
EEEEEECCCCCCCCHHHHHHHHHHHHHHCCEEEEEECCCCCCCCHHHHHHHHHHHCCCCC
DGLIVGDFALHGMHDALMRVETPTVFIGNLPNHAVDCVRIDDFGGGYQMGAYLAGKGHRR
CEEEEEHHHHHHHHHHHHHCCCCEEEECCCCCCCEEEEEEECCCCCEEECEEEECCCCCE
IAHVTGPSFFAEAMSRADGFEQGLADHGATPDPGLRFEGSYLAPSGKAAAEWLLAGHGGD
EEEECCHHHHHHHHHHHCHHHHHHHHCCCCCCCCCEECCCEECCCCHHHHHHHHCCCCCC
LPSAVFFGNYLMAAGALAEFYDRGIRIPDDFKVAVFGDQPQLEYVRPRLTRVGNAPAELA
CCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCHHHHHHHHHHCCCCHHHHH
RRATRMLLERINGTYEGEPRSEVVSCVLREFETA
HHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA