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The map label for this gene is cca [H]

Identifier: 150397472

GI number: 150397472

Start: 2347670

End: 2348929

Strand: Reverse

Name: cca [H]

Synonym: Smed_2272

Alternate gene names: 150397472

Gene position: 2348929-2347670 (Counterclockwise)

Preceding gene: 150397473

Following gene: 150397470

Centisome position: 62.11

GC content: 64.05

Gene sequence:

>1260_bases
ATGACTTCCATTGCCGCCGAGCCCTGGTTCCAGGCTCCTCAGCTTCGCCGCATATTCGACCTGTTGAATGCCGATGGCGG
CGAGGTTCGGATCGTCGGAGGGGCAATCCGCAACAGTCTGATGGGGTTGCCGGCCGGTGACGTCGACCTGGCAACCACTT
GGCATCCCGAGGATGTGATCCAGCGGGCGAGGGGCGCCGGTATCAAATCGGTGCCGACGGGGATCGACCATGGCACGGTG
ACACTCGTCATCGAAGGTATGCCCTATGAGGTGACGACATTGCGCCGCGATGTCGCAACGGACGGGCGTCGCGCCGAAGT
GGCCTTCGGGGCCGACTGGAAGGCGGATGCGCAACGCCGCGACTTCACCATCAATGCTCTTTATGGAAATGCGGAAGGCG
AGGTCCTAGACCACGTTGGCGGCCTCGATGACATCGCAACGCGCACGGTGCGTTTCATCGGCAGCGCGGCCGAGCGGGTC
GCCGAGGATTATCTCCGCATCCTGCGCTTCTTTCGCTTCTTCGCGCATTACGGCAGCGGCCGTCCCGATGCGGAGGGCCT
GAAGGCCTGTGCGCAGGCGCGCACGAAACTGGCGACGCTTTCCGCCGAACGCGTCTGGACGGAGACGAAGAAACTGCTTT
CGGCCGTGGATCCGGGCCGTGCGCTTCTATGGATGCGGCAGGCAGGCGTGCTGGGCGAGGTTCTGCCGGAGACGGAGAAA
TGGGGGATCGACGCGATCCCGGCGTTGATTTCAGCGGAGAAGAGTTTCTCCTGGCATCCGGACCATCTCCTGAGGCTTGC
TGCAATGGTCCCGCCCGACGTCGACCGGCTTGAGGCCATGGCGAAACGTCTGCGGTTGTCGAAAGCCGAGAGCGCTTACC
TCGACCGTTTCGCCAAGGCGCCGGCAATTGCGCCGGCACTTGCGGATGCGGCGCTCGATCGCCAACTCTATCGCCATGGC
TCCGAGGGCATCATCGTCCGGCTGAAGCTGGCGCTTGCTGCTGCGCGCCGGAAGGCCGAGACCGATCCCGCAATGCTAGC
CGAGAGCGCTTCCTTTCAGCGGCTCCTTGCGCGGGCGGACAAATGGCAGCGGCCCGTATTTCCGTTGAGCGGGGCCGATG
TGCTCGAAGCGGGGATACCCGCCGGTCCGAGAGTGGGAGAAGTGCTGGCCGAACTCGAAAATTTGTGGATCGAGCGCAAC
TTCAGCGTCGAACGGGCCAAACTCGTCGCCCGACTTGAGACGTTGGCGCGGCCAGGCTGA

Upstream 100 bases:

>100_bases
AGGGCGCGGAGCGCCATTTCTACCGTATGCCCTATGGCGAAAGAAACATCTGGGGGATCACCGCAGGGATCGTCCGCATG
CTCTATGAAAGGCTCTATGC

Downstream 100 bases:

>100_bases
CACGAGGTGCCGAGCCGGCCGCGATCCACCGGTCAGGCGACCTTGGTTTCGTCGACGATGCGAGCCTTGATGTTGTCGAC
CATGTTTTCGCGAATGATCG

Product: polynucleotide adenylyltransferase region

Products: NA

Alternate protein names: tRNA CCA-pyrophosphorylase; tRNA adenylyl-/cytidylyl- transferase; tRNA nucleotidyltransferase; tRNA-NT [H]

Number of amino acids: Translated: 419; Mature: 418

Protein sequence:

>419_residues
MTSIAAEPWFQAPQLRRIFDLLNADGGEVRIVGGAIRNSLMGLPAGDVDLATTWHPEDVIQRARGAGIKSVPTGIDHGTV
TLVIEGMPYEVTTLRRDVATDGRRAEVAFGADWKADAQRRDFTINALYGNAEGEVLDHVGGLDDIATRTVRFIGSAAERV
AEDYLRILRFFRFFAHYGSGRPDAEGLKACAQARTKLATLSAERVWTETKKLLSAVDPGRALLWMRQAGVLGEVLPETEK
WGIDAIPALISAEKSFSWHPDHLLRLAAMVPPDVDRLEAMAKRLRLSKAESAYLDRFAKAPAIAPALADAALDRQLYRHG
SEGIIVRLKLALAAARRKAETDPAMLAESASFQRLLARADKWQRPVFPLSGADVLEAGIPAGPRVGEVLAELENLWIERN
FSVERAKLVARLETLARPG

Sequences:

>Translated_419_residues
MTSIAAEPWFQAPQLRRIFDLLNADGGEVRIVGGAIRNSLMGLPAGDVDLATTWHPEDVIQRARGAGIKSVPTGIDHGTV
TLVIEGMPYEVTTLRRDVATDGRRAEVAFGADWKADAQRRDFTINALYGNAEGEVLDHVGGLDDIATRTVRFIGSAAERV
AEDYLRILRFFRFFAHYGSGRPDAEGLKACAQARTKLATLSAERVWTETKKLLSAVDPGRALLWMRQAGVLGEVLPETEK
WGIDAIPALISAEKSFSWHPDHLLRLAAMVPPDVDRLEAMAKRLRLSKAESAYLDRFAKAPAIAPALADAALDRQLYRHG
SEGIIVRLKLALAAARRKAETDPAMLAESASFQRLLARADKWQRPVFPLSGADVLEAGIPAGPRVGEVLAELENLWIERN
FSVERAKLVARLETLARPG
>Mature_418_residues
TSIAAEPWFQAPQLRRIFDLLNADGGEVRIVGGAIRNSLMGLPAGDVDLATTWHPEDVIQRARGAGIKSVPTGIDHGTVT
LVIEGMPYEVTTLRRDVATDGRRAEVAFGADWKADAQRRDFTINALYGNAEGEVLDHVGGLDDIATRTVRFIGSAAERVA
EDYLRILRFFRFFAHYGSGRPDAEGLKACAQARTKLATLSAERVWTETKKLLSAVDPGRALLWMRQAGVLGEVLPETEKW
GIDAIPALISAEKSFSWHPDHLLRLAAMVPPDVDRLEAMAKRLRLSKAESAYLDRFAKAPAIAPALADAALDRQLYRHGS
EGIIVRLKLALAAARRKAETDPAMLAESASFQRLLARADKWQRPVFPLSGADVLEAGIPAGPRVGEVLAELENLWIERNF
SVERAKLVARLETLARPG

Specific function: Catalyzes the addition and repair of the essential 3'- terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing

COG id: COG0617

COG function: function code J; tRNA nucleotidyltransferase/poly(A) polymerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family. Bacterial CCA-adding enzyme type 3 subfamily [H]

Homologues:

Organism=Homo sapiens, GI155030240, Length=416, Percent_Identity=31.0096153846154, Blast_Score=200, Evalue=2e-51,
Organism=Escherichia coli, GI1789436, Length=263, Percent_Identity=30.7984790874525, Blast_Score=89, Evalue=6e-19,
Organism=Escherichia coli, GI87081691, Length=205, Percent_Identity=34.1463414634146, Blast_Score=84, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI71995920, Length=410, Percent_Identity=32.4390243902439, Blast_Score=197, Evalue=6e-51,
Organism=Drosophila melanogaster, GI281360112, Length=412, Percent_Identity=33.0097087378641, Blast_Score=189, Evalue=2e-48,
Organism=Drosophila melanogaster, GI21357337, Length=412, Percent_Identity=33.0097087378641, Blast_Score=189, Evalue=2e-48,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023068
- InterPro:   IPR002646 [H]

Pfam domain/function: PF01743 PolyA_pol [H]

EC number: =2.7.7.21; =2.7.7.25 [H]

Molecular weight: Translated: 45876; Mature: 45745

Theoretical pI: Translated: 7.79; Mature: 7.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSIAAEPWFQAPQLRRIFDLLNADGGEVRIVGGAIRNSLMGLPAGDVDLATTWHPEDVI
CCCCCCCCCCCCHHHHHHHHHHCCCCCEEEEECHHHHHHHCCCCCCCCCCEECCCHHHHH
QRARGAGIKSVPTGIDHGTVTLVIEGMPYEVTTLRRDVATDGRRAEVAFGADWKADAQRR
HHHHCCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHCCCCEEEEEECCCCCCCCCCC
DFTINALYGNAEGEVLDHVGGLDDIATRTVRFIGSAAERVAEDYLRILRFFRFFAHYGSG
CEEEEEEECCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
RPDAEGLKACAQARTKLATLSAERVWTETKKLLSAVDPGRALLWMRQAGVLGEVLPETEK
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCHHHHHCCCHHH
WGIDAIPALISAEKSFSWHPDHLLRLAAMVPPDVDRLEAMAKRLRLSKAESAYLDRFAKA
CCCHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC
PAIAPALADAALDRQLYRHGSEGIIVRLKLALAAARRKAETDPAMLAESASFQRLLARAD
CCHHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
KWQRPVFPLSGADVLEAGIPAGPRVGEVLAELENLWIERNFSVERAKLVARLETLARPG
HHCCCCCCCCCCHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
TSIAAEPWFQAPQLRRIFDLLNADGGEVRIVGGAIRNSLMGLPAGDVDLATTWHPEDVI
CCCCCCCCCCCHHHHHHHHHHCCCCCEEEEECHHHHHHHCCCCCCCCCCEECCCHHHHH
QRARGAGIKSVPTGIDHGTVTLVIEGMPYEVTTLRRDVATDGRRAEVAFGADWKADAQRR
HHHHCCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHCCCCEEEEEECCCCCCCCCCC
DFTINALYGNAEGEVLDHVGGLDDIATRTVRFIGSAAERVAEDYLRILRFFRFFAHYGSG
CEEEEEEECCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
RPDAEGLKACAQARTKLATLSAERVWTETKKLLSAVDPGRALLWMRQAGVLGEVLPETEK
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCHHHHHCCCHHH
WGIDAIPALISAEKSFSWHPDHLLRLAAMVPPDVDRLEAMAKRLRLSKAESAYLDRFAKA
CCCHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC
PAIAPALADAALDRQLYRHGSEGIIVRLKLALAAARRKAETDPAMLAESASFQRLLARAD
CCHHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
KWQRPVFPLSGADVLEAGIPAGPRVGEVLAELENLWIERNFSVERAKLVARLETLARPG
HHCCCCCCCCCCHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12663927 [H]